Nanolive 3D Cell Explorer-fluo User Manual page 45

10. Software STEVE
Acquiring fluorescence images
TIP
To be able to acquire good fluorescence images, follow our guidelines hereafter.
1. At the time the dialogue appears you are able to adjust the fluorescence channel parameters, in order to acquire the best image possible
(Figure 30).
Figure 30 Dialogue window fluorescence parameters
Live-image of the selected channel
Tab for setting-up each of the selected channels
Tab for activating the white-light mode and perform bright-field of the sample
Exposure setting of the camera in ms
Gain setting of the camera in percentage: it is recommended not to go beyond 40% to acquire a
high-quality image.
Intensity setting of the LED source in percentage: it is recommended to operate at about 10%
for the sample screening to limit the sample photobleaching
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