Steps; Cleaning The Sample Probe - Agilent Technologies 4200 FlexScan Operation Manual

Analyzing Samples
The single reflection ATR is most suitable for higher absorbing
samples such as rubber, polymers, paints and fibers. Powder and
solid samples are best measured on the single reflection ATR as well
due to the optional sample press device that can apply high pressure
to powder and solid samples to ensure good contact with the
diamond sampling surface. The single reflection ATR is also a good
choice when the amount of available sample is limited. The single
reflection diamond is 2 mm in diameter with a 200 μm active area
and provides an approximate 2 micron depth of penetration for IR
energy at 1700 cm
slightly above the probe's metal mounting plate.
Although diamond is a very hard material, the ATR window is relatively thin and
CAUTION
can crack under extreme pressure conditions. Make sure the sample makes
contact with the entire surface area of the diamond and not just a point within
the diamond. Avoid the use of the sample press on samples that may be sharp or
pointed.

Steps

The steps involved in performing an ATR analysis are:
1
2
3

Cleaning the sample probe

Before using the 4200 FlexScan FTIR ATR system on a new sample, it
is important as a first step to ensure the sampling surface is clean.
Kimwipes or a suitable substitute (such as cotton swabs) should be
used to clean the sensor and sampling area. Clean the sample
interface with a suitable solvent such as acetone, methanol, ethanol
or isopropyl alcohol. Visually inspect the probe tip after cleaning to
make sure there is no film, haze or other residue present.
28
-1
. The single reflection ATR element protrudes
Clean the ATR sample mounting window.
Collect a background spectrum.
Collect a sample spectrum.
Agilent 4200 FlexScan FTIR Operation Manual