Collecting A Sample Spectrum - Agilent Technologies 4200 FlexScan Operation Manual

Analyzing Samples
To collect a background spectrum:
1
2
3

Collecting a sample spectrum

To collect a sample spectrum:
1
It is recommended to use the provided strap to secure the 4200 FlexScan FTIR to
NOTE
the user and avoid damage due to dropping the system. To install the strap, hook
both ends (2 spring clips) of strap to the rod on the lower back panel of 4200
FlexScan (just above the handle). Place the strap around the shoulder or neck.
2
26
From the software, choose a method and click Start to begin the
measurement.
The software will collect a background automatically if the
method selected was set up as recommended in the paragraph
above. The software may prompt you to install the reference cap.
Install the appropriate gold reflectance reference cap tightly
against the tip of the sample probe. The reference cap has a black
nylon strap with Velcro that can be used for convenience and
security. To install, slide the nylon strap into the loop located on
the front of the 4200 FlexScan FTIR near the sample interface.
Overlap the strap around the loop and secure using the Velcro at
the end of the strap.
For detailed software instructions for collecting a background,
refer to the MicroLab Software Operation Manual.
Once the background is finished collecting, the software will
instruct you to put the probe in contact with the sample.
The software will now display a graph showing the intensity
(absorbance) of the sample measurement. This screen is used to
ensure that the sample has been correctly placed at the sampling
interface. For 4200 FlexScan FTIR applications using reflectance,
the value should be between 0.01 and 2 AU. Use the real-time
data viewer in the software to ensure good contact between the
sample and the probe, and then choose to collect the data using
the 4200 FlexScan FTIR software.
Agilent 4200 FlexScan FTIR Operation Manual