Collecting A Sample Spectrum - Agilent Technologies 4200 FlexScan Operation Manual

Analyzing Samples

Collecting a sample spectrum

To collect a sample spectrum using the ATR probe:
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Place a small amount of material to be measured against the
diamond ATR crystal. The crystal is the clear, circular shaped
material held in place by the surrounding metal disk at the end of
the probe.
For liquid samples, simply immerse the tip of the sample
interface in the sample. Small samples can be measured by
placing a drop of sample on the center of the diamond interface.
Solid samples require establishing good contact between the
diamond sampling interface and the sample. Press the diamond
firmly into the sample. Tilt the instrument to ensure that the
diamond surface is flat to the sample.
In the case of a volatile sample, larger amounts of sample can be
applied without concern of leakage or damage to the instrument.
However, using the smallest amount of sample possible will ease
in the cleaning process.
In all cases, please be sure that the sample covers the entire
surface area of the diamond crystal.
If the sample is a powder or solid, the optional sample press may
be needed to ensure good contact with the sample.
Use the real-time data viewer in the software to ensure good
contact between the sample and the diamond, and then choose to
collect the data using the 4200 FlexScan FTIR software.
Click the NEXT button on the software screen to proceed with
the analysis.
For detailed software instructions for measuring a sample, refer
to the MicroLab Software Operation Manual.
After completing the sample measurement, immediately clean the
sample from the accessory using the instructions provided above.
It is important to make sure the sample mounting window is free
of any residue from the previous sample.
Agilent 4200 FlexScan FTIR Operation Manual