Collecting A Background Spectrum - Agilent Technologies 4200 FlexScan Operation Manual

Do not break the spectrometer seal and attempt to clean interior surfaces.
CAUTION
Breaking the seal will void the warranty.

Collecting a background spectrum

To ensure accuracy of the measurement, it is recommended that the
system is configured in the software to collect a background
spectrum before every sample is analyzed. This provides a baseline
profile of the system conditions with no sample loaded on the
instrument. By collecting a background automatically before each
sample measurement, negative effects of changes in the environment
can be avoided.
To collect a background spectrum:
1
2
To ensure an accurate background spectrum is collected, perform a visual
CAUTION
inspection of the diamond ATR sample mounting surface, looking for any haze or
film present on the diamond from measuring the previous sample. If a film is
observed, repeat the cleaning procedure above until the diamond surface is clear
of any residue.
Agilent 4200 FlexScan FTIR Operation Manual
From the software, choose a method and click Start to begin the
measurement.
The software will collect a background automatically if the
method selected was set up as recommended in the paragraph
above.
The diamond should not be in contact with the sample or any
substance other than the air in the atmosphere during the
background measurement.
For detailed software instructions for collecting a background,
refer to the MicroLab Software Operation Manual.
Analyzing Samples
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