Fisher Scientific MARATHON 3000 Operation Manuals
Fisher Scientific MARATHON 3000 Operation Manuals

Fisher Scientific MARATHON 3000 Operation Manuals

General purpose centrifuge

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Fisher Scientific MARATHON 3000 Operation Manuals

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Summary of Contents for Fisher Scientific MARATHON 3000

  • Page 1 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...
  • Page 2 2000 Park Lane Pittsburgh, PA 15275 Tel. (800) 766-7000 Fax (800) 926-1166 MARATHON 3000(R) General Purpose Centrifuge Operation Manual Model 3000 3L Ventilated Centrifuge Cat. No. 04-977-3000 -- 120 VAC, 60 Hz Cat. No. 04-977-3001 -- 220, 230, 240 VAC, 50/60 Hz Model 3000R 3L Refrigerated Centrifuge Cat.
  • Page 3: Table Of Contents

    Table of Contents Section 1 - Introduction Page 1.1 General Product Description....................4 1.2 About This Manual ......................5 1.3 Warnings, Cautions and Notes....................6 Section 2 - Installation 2.1 Receiving the Unit ......................7 2.2 Site Preparation........................7 2.3 Power Configuration......................9 2.4 Moving the Unit.........................10 2.5 The Front Panel (Keys and Displays) ................11 Section 3 - Operation 3.1 Rotor and Accessories .......................15...
  • Page 4 Copyright ©, 1998 Fisher Scientific Printed in the USA MARATHON Series Operation Manual Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 5: General Product Description

    Marathon series units are low-speed, general purpose centrifuges, used in medical, industrial, and scientific applications. The Marathon 3000 Series is available in two models: ventilated (3000) and refrigerated (3000R). Sections of this manual that apply to the refrigerated version will be designated 3000R or refrigerated only.
  • Page 6: About This Manual

    The Marathon Series features a maintenance-free, brushless motor, and an easy-to-use front panel, which provides three versatile timing modes: automatic timed run, short spin (momentary), and hold (continuous mode). Acceleration and brake rates may be controlled, to optimize runs: rapid for fast separations or slow for delicate samples.
  • Page 7: Warnings, Cautions And Notes

    Warnings, Cautions, and Notes The terms warning, caution, and note have specific meanings in this manual. • A Warning advises against certain actions or situations that could result in personal injury. • A Caution advises against actions or situations that could damage equipment, produce inaccurate data, or invalidate a procedure.
  • Page 8: Section 2 - Installation

    2 Installation Receiving the Unit Fisher Scientific ships the centrifuge in a carton that protects it from shipping hazards. Follow the unpacking instructions on the carton. Be sure to complete and return the postage-paid warranty card. Site Preparation The unit normally resides on a bench top. The 3000 (ventilated model) can be placed in a cold room (no colder than 4°C), for processing temperature-sensitive samples.
  • Page 9 The following table lists the physical dimensions for the 3000 and 3000R: 3000R 3000 Sample Loading Height 14.7” (37.3 cm) 14.7” (37.3 cm) 16.5” (41.9 cm) 16.5” (41.9 cm) Cover Closed Height 41” (104.1 cm) 41” (104.1 cm) Cover Open Height Width 30”...
  • Page 10: Power Configuration

    Power Configuration The Marathon Series uses AC power in different configurations, appropriate for use throughout the world. Please check the catalog number of the model that you have purchased, to ensure that the machine you have is the proper power configuration. For best results, the refrigerated centrifuge, 3000R, should be used on a dedicated line.
  • Page 11: Moving The Unit

    Circuit Breaker The system provides an automatic circuit breaker, for emergency situations, such as power surges, that could damage the unit. If the circuit breaker trips: 1. Unplug the unit. 2. Press the white button, on the left side of the unit. 3.
  • Page 12: The Front Panel (Keys And Displays)

    The Front Panel The Front Control Panel (3000R Model Shown) The control panel contains numeric displays for RPM/RCF (Speed/Force), Time, program, and Temperature (Refrigerated only). These displays have two states or modes: Actual (bright display) and Set (dim display). In Actual mode (bright display), they indicate current run conditions, such as: •...
  • Page 13 SPEED Speed Key: Pressing the SPEED key switches the display from Actual to Set mode. Select the desired speed using the numeric keypad, and then press ENTER. A selection must begin before 5 seconds elapse or the display will revert to the Actual reading.
  • Page 14 TEMP Temperature key: Pressing the TEMP key switches the (3000R only) display from Actual to Set mode. Select the desired temperature using the numeric keypad, and then press ENTER. A selection must begin before 5 seconds elapse or the display will revert to the Actual reading. Temperature display: The number in the display represents temperature in degrees Celsius, from −...
  • Page 15 Acceleration and Braking Gentle acceleration and braking can be selected, when centrifuging delicate samples. The gentle settings avoid mixing of density gradients or breakup of pellets. ACCEL Acceleration key: The Acceleration key controls rotor acceleration up to 400 RPM. Press this key to toggle between fast and slow acceleration.
  • Page 16: Section 3 - Operation

    3 Operation Rotor and Accessories A balanced load is essential for all centrifuges. An unbalanced load produces vibration, and can damage the unit. A 2 gram load imbalance, at a speed of 4600 RPM, imparts force equivalent to 9.1 kg (20 pounds) at rest. Always ensure that the rotor is loaded symmetrically, with a full complement of accessories, and a full (or paired) set of tubes.
  • Page 17 Tubes loaded into swinging bucket rotors must be symmetric, around the axis of rotation. Verify this by rotating the entire rotor 180°, by hand. The loads should be in the same apparent positions (not mirror images). In addition, the loads within each bucket must, also, be symmetric around the bucket’s pivot axis.
  • Page 18 Vibration All centrifuges have critical speeds, at which vibration occurs. As the speed increases, beyond the critical speed, vibration will cease. This inherent condition, also, occurs during deceleration. An imbalanced load intensifies these critical vibrations. Do not continuously operate this centrifuge at observed critical speeds.
  • Page 19: Starting And Stopping A Run

    3. Lift the rotor off the shaft. Starting and Stopping a Run Read Section 2.5, for a general description of the front panel. The settings displayed on the front panel always govern the operation of the unit. The display above the Program key shows the unit’s operating mode.
  • Page 20: Stored Programs

    The program will remain in memory until further changes are made. Because the Marathon 3000(R) has a fully automatic rotor recognition system, the very first rotor that is spun using a particular program will be identified and have its identity automatically stored in the program.
  • Page 21 together with its default radius (if not explicitly entered to the program). To make changes temporary, press START without pressing the SAVE key. The program display will flash, to indicate that the instrument is not currently operating from program mode. The original program will remain unchanged as long as the SAVE key is not pressed.
  • Page 22: Rotor Recognition System

    Rotor Recognition System The Marathon 3000(R) is equipped with a fully automatic rotor recognition system that internally detects and identifies the rotor installed. Since the Marathon 3000(R) was designed to spin a variety of rotors, its software database contains the maximum settable values for speed, RCF, and radius for each.
  • Page 23: 3000R Refrigeration

    Refrigeration (3000R only) Refrigerated units refrigerate the rotor chamber whenever the cover is closed and the unit is switched on. Refrigeration is applied, as necessary, to cool the rotor chamber to the currently displayed temperature setting. If you use the key pad, and momentarily display a cold temperature (stepping through stored programs, for example), refrigeration will not be activated.
  • Page 24: Diagnostic Messages And Error Codes

    Diagnostic Messages and Error Codes The beeper sounds in these situations • Two times on power up. • Three times at the end of a spin. • Five times when a warning occurs Diagnostics Diagnostic messages appear in place of the speed display, in the following cases: Message Description...
  • Page 25 Drive Fault The drive fault error can occur due to one of the following conditions: • the motor experiences an abnormally high current or voltage condition • the drive PCB operating temperatures exceed 70 • a brake circuit fault has been detected •...
  • Page 26 Error Codes An error code typically means that the internal microprocessor has detected impermissible readings, or a failure, elsewhere, in the unit. Error messages appear in the speed display. When an error code is displayed, unplug and reconnect the unit to power. If the error code reappears, factory-authorized maintenance may be required.
  • Page 27: Section 4 - Applications

    4 Applications Introduction This section describes the use of specific rotors and accessories. More detailed information is shipped with the rotor or accessory itself. This section contains five reference sections: • Speed and Force Tables • Derating Table for Dense Samples •...
  • Page 28 Use of any tube above its rated RCF can cause tube cracking. To avoid this, compare the G forces, specified in the Speed and Force Tables, with the ratings for the tubes that you are using. If the tubes are not rated for the force that the centrifuge will apply, look up their reduced G force, and enter it on the control panel.
  • Page 29: Speed And Force Tables

    Speed and Force Tables Rotor 04-976-008 4-Place Swinging Bucket, 4x750 ml Complete with 4 buckets and 1 mounted rotor recognition disk, but without Sealed tube adapters Tube Maximum Adapter* No. x Vol. (ml) Description RPM / RCF Radius Quantity x Cat. No. 4 x 750ml unsealed 05-112-76 bottle 3400...
  • Page 30 Rotor 04-976-007 6-Place Swinging Bucket, 6x250ml Complete with 4 buckets and mounted rotor recognition disk, but without Sealed tube adapters Tube Maximum Sealed Adapter* No. x Vol. (ml) Description RPM / RCF Radius Quantity x Cat. No. 6 x 250ml sealed 05-432A, 05-432B bottles 3600 3030...
  • Page 31 Pear-Shaped Oil Tube Rotor 04-976-013 4-Place Swinging Bucket, 4x100ml Complete with 4 removable buckets with cushions and 1 mounted rotor recognition disk. Tube Maximum No. x Vol. (ml) Description RPM / RCF Radius 4 x 100ml 05-620, 05-622 pear-shaped tubes 2500 1350 19.2...
  • Page 32: Derating Tables

    Derating Tables Dense Samples The Speed and Force Tables list the maximum speed for each rotor/adapter combination for the Marathon Series. These speeds are specified for samples whose specific gravity is not greater than 1.2, for swinging bucket rotors. For denser samples, the maximum specified speed is reduced (derated) by a factor from the table below: Derating Factor for: Specific Gravity...
  • Page 33 Specific gravities greater than 3.0. This table is based on the formula: You can use the same formula to compute derating factors for specific gravities greater than 3.0. • S is the maximum specific gravity allowed before derating (1.2). • S is the actual specific gravity of the sample.
  • Page 34: Chemical Resistance Tables

    Chemical Resistance Table The Marathon Series is made of materials that are designed to resist attack from most laboratory chemicals. A variety of materials, including aluminum and polypropylene, comprise the rotors and accessories. The Chemical Resistance table shows the suitability of each material, with different classes of reagents.
  • Page 35: Decontamination Table

    Decontamination Table Compatible Processes For Decontamination Sterilization Methods Plastic Metal Other PA PC PE PP PU NL DN CN NN PS TI SS AL MB MG RR BN VN PF PT Mechanical Autoclave* Ethylene Oxide Gas Dry Heat (2Hrs. @ 160°C) Chemical Ethanol 40% Formalin...
  • Page 36: Rcf Nomograph

    RCF Nomograph MARATHON Series Operation Manual Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 37: Section 5 - Maintenance

    5 Maintenance Introduction This chapter explains how to keep your unit in good operating order. It includes instructions for cleaning, decontaminating, and storing. This chapter, also, covers the cover interlock bypass. See the end of the chapter, for information on service and warrantees.
  • Page 38 Scrub the rotor’s tube cavities with a stiff test tube brush that has end bristles and a non-metallic tip. Dry each part, after cleaning, with a clean, absorbent towel. If glass breakage occurs, remove all broken pieces immediately. If breakage recurs, replace all adapters and cushions.
  • Page 39 Storage Store parts on a soft surface, to avoid damage. Rotors and other parts should be clean and dry. Store them open to the air, not in a plastic bag, so that any residual moisture evaporates. Face the parts upward, to avoid moisture retention in the cavities.
  • Page 40: Cover Interlock Bypass

    Cover Interlock Bypass The cover will remain locked, if power fails. If you need to remove samples from the unit, before power is restored, use the cover interlock bypass, after the rotor has come to a stop. To bypass the cover interlock: 1.
  • Page 41: Condition Of Returned Equipment

    You must prepay transportation to the service depot. Warranty Fisher Scientific wants you to be satisfied with the quality of your Marathon Series centrifuge. We warranty the centrifuge for one year, and rotors for seven years. We will...
  • Page 42: Section 6 - Specifications

    6 Specifications Maximum Speed: 3,600 RPM Maximum G Force: 3,000 xg Maximum Rotating Radius: 21.0 cm Maximum Capacity: 3 liter (4 x 750 ml) Sound Level: 3000 Max. 57 dbA (RMS) 3000R Max. 62 dbA (RMS) w/ compressor on Operator Controls Chamber Temperature: 3000R only -9°...
  • Page 43 Refrigerant: non-CFC 3000R only R-22 (7.5 oz. Lo-V Models) R-22 (6.5 oz. Hi-V Models) Operating pressures: 150 psi (low side) at 4° C (3000R only) 300 psi (high side) Max sample temperature rise: 3000 only 7° C above ambient Power Requirements and Output Description Electrical Requirements 6459…...
  • Page 44 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...

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