Related Manuals for Fisher Scientific Finnpipette
Summary of Contents for Fisher Scientific Finnpipette
- Page 1 Fisherbrand ® Finnpipette ® INSTRUCTIONS FOR USE 2000 PARK LANE Pittsburgh PA 15275 800-766-7000...
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Page 2: Table Of Contents
CONTENTS PRODUCT DESCRIPTION PIPETTE OPERATION PIPETTING TECHNIQUES 4, 5, 6 CALIBRATION 7, 8, 9 TROUBLE SHOOTING MAINTENANCE SPARE PARTS LIST FIGURE 1 PARTS DIAGRAM 14, 15 CONVERSION TABLE... -
Page 3: Product Description
PRODUCT DESCRIPTION The Fisherbrand Finnpipette Digital, Multichannel, Manual Pipetter operates on the air ® displacement principle and uses disposable polypropylene pipette tips. Five models cover a volume range from 0.5µl to 300µl. CONSTRUCTION The tough polyamide and nylon pipetter handle stands up to volatile and aggressive liquids. -
Page 4: Pipette Operation
PIPETTER OPERATION SETTING THE DELIVERY VOLUME To set the delivery volume, turn the thumb knob counterclockwise to increase volume and clockwise to decrease volume. Make sure the thumb knob clicks into place for the desired volume and the digits are completely visible in the display window. Note: Do not set volumes outside the pipetter’s specified volume range.Forcing the thumb knob to turn outside the range may jam the mechanism and eventually damage the pipetter. - Page 5 PIPETTING TECHNIQUES, Contd. FORWARD TECHNIQUE A = Ready position Fill a clean reagent reservoir with the liquid to be dispensed. B = First stop C = Second stop 1. Depress the push button to the first stop. 2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1cm and slowly 1 2 3 4 release the push button.
- Page 6 REPETITIVE TECHNIQUE The repetitive technique offers a rapid and simple procedure for repeated delivery of the same volume. 1 2 3 4 Fill a clean reagent reservoir with the liquid to be dispensed. 1. Depress the push button all the way to the second stop.
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Page 7: Calibration
CHECKING THE CALIBRATION The pipetter is checked with the maximum (nominal) volume and with the minimum volume (or 10% of maximum vol-ume, whichever is higher.) For example, Finnpipette 0.5–10µl is tested at 10µl and 1µl. A new tip is first pre-wetted three to five times and a series of ten pipettings is done with each volume. -
Page 8: Calibration 7
Procedure: 1. Do 10 pipettings with the min. volume. 2. Do 10 pipettings with the max. volume. 3. Calculate the accuracy (A) and precision (cv) of both series (See formulas below.) 4. Compare the results to the limits in Table 1 on the following page. If the results are within the limits given, then the calibration of the pipetter is correct. -
Page 9: Calibration 7
Accuracy (systematic error) Accuracy is the difference between the dispensed volume and the selected volume of a pipette. A = V - V A = accuracy V = mean volume = nominal volume Accuracy can be expressed as a relative value: A% = 100% x A / V Precision (random error) Precision refers to the repeatability of the pipettings. -
Page 10: Trouble Shooting
Finnpipette is designed to allow easy in-lab service. If you ® would prefer to have Fisher Scientific service your pipetter, make sure that it has been decontaminated before shipping. Please note that the postal authorities in your country may prohibit or restrict the shipment of contaminated material by mail. -
Page 11: Maintenance
Finnpipette Digital Multichannel is not in use, make sure it ® is stored in an upright posi-tion. We recommend a Finnpipette stand for this purpose. LONG-TERM MAINTENANCE If the pipetter is used daily, it should be checked every three months. The servicing procedure starts with the disassembly of the pipetter. - Page 12 ASSEMBLING MULTICHANNEL PIPETTERS* 5–50 µl and 50–300 µl: Take one piston. Slide cover ring (32) (larger hole), spring (33), support ring (35), (o–ring (37) bigger 5-50µl) and o–ring (36) (smaller) onto the piston. Grease the o–ring with the lubricant provided in the pipette package. Slide all the parts into the tip cone and close the snap joint of the cover ring.
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Page 13: Spare Parts List
SPARE PARTS See figure 1 for spare parts and reference numbers Module Shelf hanger 8-ch 0,5-50 µl 2207180 2206740 1. 2900580 8-ch 5-50 µl 2206750 2. 10593720 8-ch 50-300 µl 2206760 3. 10594540 12-ch 0,5-10 µl 2207190 4. 1131820 12-ch 5-50 µl 2206770 12-ch... -
Page 16: Conversion Table
1.0049 1.0050 1.0050 1.0051 1.0052 29.5 1.0050 1.0051 1.0051 1.0052 1.0052 1.0053 1.0052 1.0052 1.0053 1.0053 1.0054 1.0055 For customer service, call 1-800-766-7000. To fax an order, use 1-800-926-1166. To order online: www.fishersci.com ©2000 Fisher Scientific Litho in U.S.A. BN...