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Summary of Contents for Fisher Scientific Finnpipette II
- Page 1 Digital Fisherbrand ® Variable & Finnpipette II Fixed Volume INSTRUCTIONS FOR USE 2000 PARK LANE Pittsburgh PA 15275 800-766-7000...
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Page 3: Table Of Contents
CONTENTS PRODUCT DESCRIPTION PIPETTE OPERATION PIPETTING TECHNIQUES CALIBRATION MAINTENANCE TROUBLE SHOOTING PACKAGE SPARE PARTS 12-13... -
Page 4: Product Description
PRODUCT DESCRIPTION The Fisherbrand Finnpipette II is a manual digital pipette. It operates on the air displacement ® principle (i.e. an air interface) and uses detachable, disposable tips. The adjusted delivery volume is displayed digitally on a readout window on the handle. -
Page 5: Pipette Operation
DIGITAL DISPLAY The adjusted delivery volume is clearly indicated in the large digital display on the handle. RAW MATERIALS The Fisherbrand Finnpipette II is made of mechanically durable and chemically resistant ® materials. PIPETTE OPERATION SETTING THE DELIVERY VOLUME 1. Set the delivery volume using the push button on the top of the pipette.To increase the delivery volume, turn the push button counterclockwise. - Page 6 Before you begin your actual pipetting work, fill and empty the tip 2-3 times with the solution that you will be pipetting. Hold the pipette in an upright position while aspirating liquid. The grippy should rest on your index finger. Make sure that the tips, pipette and solution are at the same temperature. FORWARD TECHNIQUE Fill a clean reagent reservoir with the liquid to be dispensed. 1. Depress the push button to the first stop. 2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm and slowly release the push button.
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Page 7: Calibration
PIPETTING OF HETEROGENEOUS SAMPLES (deproteinization in blood glucose determination, for example) Use steps 1 and 2 of the forward technique to fill the tip with blood. Wipe the tip carefully with a dry clean tissue. 1. Immerse the tip into the reagent and depress the push button to the first stop, making sure the tip is well below the surface. 2. Release the push button slowly to the ready position. This will fill the tip. Keep the tip in the solution. 3. Depress the push button to the first stop and release slowly. Keep repeating this procedure until the interior wall of the tip is clear. 4. - Page 8 TABLE 1: Maximum permissible errors according ISO8655 Range Volume Accuracy Precision Volume Accuracy Precision µl µl s.d.µl µl µl s.d.*µl cv% 0,2-2µl ±0.080 ±4 0.040 ±0.04 ±4.0 0.04 ±0.080 ±40 0.020 20.0 ±0.05 ±2.5 0.04 0,5-10µl ±0.120 ±1.2 0.080 ±0.07 ±1.4 0.07 ±0.120...
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Page 9: Maintenance
Standard deviation can be expressed as a relative value (CV) CV = 100% x S / V MAINTENANCE When the Fisherbrand Finnpipette II is not in use, make sure it is stored in an upright ® position. We recommend a Finnpipette stand for this purpose. - Page 10 9. Reassemble the pipette components. All 0.2-50µl / 1-40µl: First, slide the spring 14, spring support 15 and tube 16 back on the piston. Compress the spring with fingers by pressing piston and spring support 15 agains each other. 5-50µl / 11-40µl: Slide bigger O-ring 17, smaller O-ring 18, (2-20µl bigger O-ring 18a, smaller O-ring 18b), spring support 19 (sharp edges against spring) and small spring 20 on the piston.
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Page 11: Trouble Shooting
High viscosity liquids may question with certain require recalibration liquids PACKAGE The Fisherbrand Finnpipette II is shipped in a specially designed package containing the ® following items: 1. The Finnpipette 5. Instruction manual 2. Service tool 6. Calibration certificate 3. Maintenance pliers 7. Shelf hanger 4. -
Page 14: Spare Parts
SPARE PARTS Figure 15 lists spare parts and reorder numbers 0,5-10µl / 3-10µl Fixed 5-50µl / 11-40µl Fixed 5. 10592510 1. 10593720 10. 10593410 10592500 2. 2900580 11. 10593630 5. 10593960 Fix 20µl 10593990 Fix 5µl 3. 10593050 12. 10593100 5. - Page 15 Fisherbrand ® Finnpipette II is designed to allow easy in-lab service. If you would prefer to have us or your local representative service your pipette, please make sure that the pipette has been decontaminated before you send it to us.
- Page 16 For customer service, call 1-800-766-7000. To fax an order, use 1-800-926-1166. To order online: www.fishersci.com ©2002 Fisher Scientific Litho in U.S.A. BN...
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