Fisher Scientific MARATHON 21000 Operation Manual

Ventilated centrifuge

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2000 Park Lane Pittsburgh, PA 15275

Tel. (800) 766-7000 Fax (800) 926-1166

MARATHON 21000(R)

Multi Purpose Centrifuge

Operation Manual

Model 21000 1L Ventilated Centrifuge

Cat. No.

Model 21000R 1L Refrigerated Centrifuge

Cat. No.

Part No. 04-977-21000M Rev. 4

04-977-21000 -- 120 VAC, 60 Hz

04-977-210001 -- 220, 230, 240 VAC, 50/60 Hz

04-977-21000R -- 120 VAC, 60 Hz

04-977-21001R -- 220, 230, 240 VAC, 50 Hz

04-977-21002R -- 230 VAC, 60 Hz

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Page 1 2000 Park Lane Pittsburgh, PA 15275 Tel. (800) 766-7000 Fax (800) 926-1166 MARATHON 21000(R) Multi Purpose Centrifuge Operation Manual Model 21000 1L Ventilated Centrifuge Cat. No. 04-977-21000 -- 120 VAC, 60 Hz Cat. No. 04-977-210001 -- 220, 230, 240 VAC, 50/60 Hz Model 21000R 1L Refrigerated Centrifuge Cat.
Page 2: Table Of Contents
Table of Contents 1 INTRODUCTION 1.1 G ..................4 ENERAL RODUCT ESCRIPTION 1.2 A ......................5 BOUT ANUAL 1.3 W ..................6 ARNINGS AUTIONS OTES 2 INSTALLATION 2.1 R ......................7 ECEIVING THE 2.2 S ......................... 7 REPARATION 2.3 P ......................
Page 4: Introduction
Marathon series units are high-speed, multi purpose centrifuges, used in medical, industrial, and scientific applications. The Marathon 21000 Series is available in two models: ventilated (21000) and refrigerated (21000R). Sections of this manual that apply to the refrigerated version will be designated 21000R or refrigerated only.
Page 5: About This Manual
The Marathon Series features a maintenance-free, brushless motor, and an easy-to-use front panel, which provides three versatile timing modes: automatic timed run, short spin (momentary), and hold (continuous mode). Acceleration and brake rates may be controlled, to optimize runs: rapid for fast separations or slow for delicate samples.
Page 6: Warnings, Cautions, And Notes
Warnings, Cautions, and Notes The terms warning, caution, and note have specific meanings in this manual. A Warning advises against certain actions or situations that could result in personal injury. A Caution advises against actions or situations that could damage equipment, produce inaccurate data, or invalidate a procedure.
Page 7: Installation
2 Installation Receiving the Unit Fisher Scientific ships the centrifuge in a carton that protects it from shipping hazards. Follow the unpacking instructions on the carton. Be sure to complete and return the postage-paid warranty card. Site Preparation The unit normally resides on a bench top. The 21000 (ventilated model) can be placed in a cold room (no colder than 4 C), for processing temperature-sensitive samples.
Page 8 The following table lists the physical dimensions for the 21000 and 21000R: 21000R 21000 13.7 (34.8 cm) 13.7 (34.8 cm) Sample Loading Height Cover Closed Height 15.5 (39.4 cm) 15.5 (39.4 cm) Cover Open Height 38.5 (97.8 cm) 38.5 (97.8 cm) 28 (71.1 cm) 20.3 (51.4 cm) Width...
Page 9: Power Configuration
Power Configuration The Marathon Series uses AC power in different configurations, appropriate for use throughout the world. Please check the catalog number of the model that you have purchased, to ensure that the machine you have is the proper power configuration. For best results, the refrigerated centrifuge, 21000R, should be used on a dedicated line.
Page 10: Moving The Unit
Circuit Breaker The system provides an automatic circuit breaker, for emergency situations, such as power surges, that could damage the unit. If the circuit breaker trips: 1. Unplug the unit. 2. Press the white button, on the left side of the unit. 3.
Page 11: The Front Panel
The Front Panel The Front Control Panel (21000R Model Shown) The control panel contains numeric displays for RPM/RCF (Speed/Force), Time, program, and Temperature (Refrigerated only). These displays have two states or modes: Actual (bright display) and Set (dim display). In Actual mode (bright display), they indicate current run conditions, such as: rotor speed or force elapsed time of, or time remaining in, the run...
Page 12 from Actual to Set mode. Select the desired speed using the numeric keypad, and then press ENTER. A selection must begin before 5 seconds elapse or the display will revert to the Actual reading. Speed/Force key and display: The number in the speed/force display represents the rotor speed in RPM or force in RCF.
Page 13 temperature using the numeric keypad, and then press ENTER. A selection must begin before 5 seconds elapse or the display will revert to the Actual reading. Temperature display: The number in the display represents temperature in degrees Celsius, from through 40 C.
Page 14 Acceleration and Braking Gentle acceleration and braking can be selected, when centrifuging delicate samples. The gentle settings avoid mixing of density gradients or breakup of pellets. ACCEL Acceleration key: The Acceleration key controls rotor acceleration up to 800 RPM. Press this key to toggle between fast and slow acceleration.
Page 15: Operation
3 Operation Rotor and Accessories A balanced load is essential for all centrifuges. An unbalanced load produces vibration, and can damage the unit. A 2 gram load imbalance, at a speed of 4600 RPM, imparts force equivalent to 9.1 kg (20 pounds) at rest. Always ensure that the rotor is loaded symmetrically, with a full complement of accessories, and a full (or paired) set of tubes.
Page 16 Tubes loaded into swinging bucket rotors must be symmetric, around the axis of rotation. Verify this by rotating the entire rotor 180°, by hand. The loads should be in the same apparent positions (not mirror images). In addition, the loads within each bucket must, also, be symmetric around the bucket s pivot axis.
Page 17 Vibration All centrifuges have critical speeds, at which vibration occurs. As the speed increases, beyond the critical speed, vibration will cease. This inherent condition, also, occurs during deceleration. An imbalanced load intensifies these critical vibrations. Do not continuously operate this centrifuge at observed critical speeds.
Page 18: Starting And Stopping A Run
Rotor Removal To remove the rotor: 1. Remove the cover (fixed angle only). 2. Using the wrench provided, loosen the rotor locking nut. Remove the wrench. 3. Remove the locking nut. 4. Lift the rotor off the shaft. Starting and Stopping a Run Read Section 2.5, for a general description of the front panel.
Page 19: Stored Programs
The program will remain in memory until further changes are made. Because the Marathon 21000(R) has a fully automatic rotor recognition system, the very first rotor that is spun using a particular program will be identified and have its identity automatically stored in the program.
Page 20 other than the one whose identity is stored in the program, a ch hd (check head) message will appear. If the user wishes to clear the rotor and its radius from the memory banks of the program, he or she need only press the SAVE button.
Page 21: Rotor Recognition System
Rotor Recognition System The Marathon 21000(R) is equipped with a fully automatic rotor recognition system that internally detects and identifies the rotor installed. Since the Marathon 21000(R) was designed to spin a variety of rotors, its software database contains the maximum settable values for speed, RCF, and radius for each.
Page 22: Refrigeration (21000R Only)
Refrigeration (21000R only) Refrigerated units refrigerate the rotor chamber whenever the cover is closed and the unit is switched on. Refrigeration is applied, as necessary, to cool the rotor chamber to the currently displayed temperature setting. If you use the key pad, and momentarily display a cold temperature (stepping through stored programs, for example), refrigeration will not be activated.
Page 23: Diagnostic Messages And Error Codes
Diagnostic Messages and Error Codes The beeper sounds in these situations Two times on power up. Three times at the end of a spin. Five times when a warning occurs Diagnostics Diagnostic messages appear in place of the speed display, in the following cases: Message Description...
Page 24 Error Codes An error code typically means that the internal microprocessor has detected impermissible readings, or a failure, elsewhere, in the unit. Error messages appear in the speed display. When an error code is displayed, unplug and reconnect the unit to power. If the error code reappears, factory-authorized maintenance may be required.
Page 25: Applications
4 Applications Introduction This section describes the use of specific rotors and accessories. More detailed information is shipped with the rotor or accessory itself. This section contains five reference sections: Speed and Force Tables Derating Table for Dense Samples Chemical Resistance Table Decontamination Table Nomograph Caution: Do not exceed maximum rated speed for each...
Page 26 Use of any tube above its rated RCF can cause tube cracking. To avoid this, compare the G forces, specified in the Speed and Force Tables, with the ratings for the tubes that you are using. If the tubes are not rated for the force that the centrifuge will apply, look up their reduced G force, and enter it on the control panel.
Page 27: Speed And Force Tables
Speed and Force Tables Rotor 04-976-006 4-Place Swinging Bucket, 4x250ml Complete with 4 buckets and rotor recognition ring, but without Sealed Tube Carriers. Tube Maximum Sealed Tube Carrier No. x Vol. (ml) Description RPM / RCF Radius Quantity x Cat. No. 4 x 250 ml sealed IEC 2502, 2504 4500...
Page 28 Rotor 04-974-009 Microplate Rotor Complete with 2 carriers and rotor recognition ring. See Section 4.3 Derating Tables, for information on derating factors for use with samples over 320 grams. Tube Maximum No. x Vol. (ml) Description RPM / RCF Radius 2 or 4 x standard 86 x 128 mm 3650...
Page 29 Rotor 04-976-011 6-Place Fixed Angle, 6x85ml Complete with wind resistant lid and rotor recognition ring, but without tube adapters. Tube Maximum Tube Adapter No. x Vol. (ml) Description RPM / RCF Radius Quantity x Cat. No. 6 x 85 mL 1 pk/10 05-529D 13700 21200...
Page 30: Derating Tables
Derating Tables Dense Samples The Speed and Force Tables list the maximum speed for each rotor/adapter combination for the Marathon Series. These speeds are specified for samples whose specific gravity is not greater than 1.2, for swinging bucket rotors. For denser samples, the maximum specified speed is reduced (derated) by a factor from the table below: Derating Factor for: Derating...
Page 31 Microplate The Microplate rotor is designed for centrifugation of multi- Sample Weight well microplates. The weight of the loaded microplates must be equally distributed between the rotor s two carriers. The maximum rated speed for the rotor, when each of the carriers is loaded with 320 grams (total load of 640 grams), is 3650 rpm.
Page 32: Chemical Resistance Table
Chemical Resistance Table The Marathon Series is made of materials that are designed to resist attack from most laboratory chemicals. A variety of materials, including aluminum and polypropylene, comprise the rotors and accessories. The Chemical Resistance table shows the suitability of each material, with different classes of reagents.
Page 33: Decontamination Table
Decontamination Table Compatible Processes For Decontamination Sterilization Methods Plastic Metal Other PA PC PE PP PU NL DN CN NN PS TI SS AL MB MG RR BN VN PF PT Mechanical Autoclave* Ethylene Oxide Gas Dry Heat (2Hrs. @ 160°C) Chemical Ethanol 40% Formalin...
Page 34: Rcf Nomograph
RCF Nomograph MARATHON Series Operation Manual...
Page 35: Maintenance
5 Maintenance Introduction This chapter explains how to keep your unit in good operating order. It includes instructions for cleaning, decontaminating, and storing. This chapter, also, covers the cover interlock bypass. See the end of the chapter, for information on service and warrantees.
Page 36 Scrub the rotor s tube cavities with a stiff test tube brush that has end bristles and a non-metallic tip. Dry each part, after cleaning, with a clean, absorbent towel. If glass breakage occurs, remove all broken pieces immediately. If breakage recurs, replace all adapters and cushions.
Page 37 Storage Store parts on a soft surface, to avoid damage. Rotors and other parts should be clean and dry. Store them open to the air, not in a plastic bag, so that any residual moisture evaporates. Face the parts upward, to avoid moisture retention in the cavities.
Page 38: Cover Interlock Bypass
Cover Interlock Bypass The cover will remain locked, if power fails. If you need to remove samples from the unit, before power is restored, use the cover interlock bypass, after the rotor has come to a stop. To bypass the cover interlock: 1.
Page 39: Condition Of Returned Equipment
You must prepay transportation to the service depot. Warranty Fisher Scientific wants you to be satisfied with the quality of your Marathon Series centrifuge. We warranty the centrifuge for one year, and rotors for seven years. We will...
Page 40: Specifications
6 Specifications Maximum Speed: 15,000 RPM Maximum G Force: 21,200 xg Maximum Rotating Radius: 17.6 cm Maximum Capacity: 1 liter (4 x 250 ml) Sound Level: 21000 Max. 66 dbA (RMS) 21000R Max. 68 dbA (RMS) w/ compressor on Operator Controls Chamber Temperature: 21000R only -9°...
Page 41 Refrigerant: non-CFC 21000R only R-404A (7.9 oz Lo-V Models) R-404A (6.9 oz Hi-V Models) Operating pressures: 20 psi (low side) at 4° C (21000R only) 250 psi (high side) Max sample temperature rise: 21000 only 7° C above ambient* *except for 6 x 85 mL Fixed Angle Rotor Power Requirements and Output Description Electrical Requirements...

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Marathon 21000r 04-977-21000 04-977-210001 04-977-21000r 04-977-21001r 04-977-21002r

Fisher Scientific MARATHON 21000 Operation Manual

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