Quality Control For The User - wtw photolab S12 Operating Instructions Manual

photoLab ® Series
5 Analytical Quality Assurance (AQA)
Chargenzertifikat
Lot Certificate / Certificado del lote
®
Spectroquant CSB-Küvettentest
® ®
Spectroquant COD Cell Test / Spectroquant Test en cubetas DQO
Art.Nr. / Cat.No. / Art. Nro. 1.14560.0001
Messbereich
4,0 - 40,0 mg/l CSB/COD/DQO
Measuring Range / Intervalo de medida
Charge-Nr. / Lot no. / Lote nro. HC119527
Verwendbarkeit
31.10.2012
Expiry date / Fecha de caducidad
Standard / Standard / Patrón Potassium hydrogen phthalate 1.02400
Photometer / Photometer / Fotómetro Referenz / Reference / Referencia
Wellenlänge / Wavelength / Longitud de onda 340 nm
Küvette / Cell / Cubeta 16 mm rund / round / redonda
Prüfer / Tester / Verificador Fr. Brandner
Datum / Date / Fecha 20.09.2011
Datei / File / Fichero 1145600001_HC119527_EN
Kalibrierfunktion / Calibration Function / Función de calibración
DIN 38402 A51 / ISO 8466-1
Steigung / Slope / Pendiente +/- Tolerance / Tolerancia
Ordinatenabschnitt / Ordinate segment / Intersecto en ordenadas
Reag.blindwert / Reagent blank / Valor en blanco del react +/- Tolerance / Tolerancia
Vertrauensbereich (95% Wahrscheinlichkeit)
Confidential interval (P=95%) / Intervalo de confianza (95 % de probabilidad)
Verfahrensstandardabweichung
Standard Deviation of the Method / Desviación estándar del procedimiento
Verfahrensvariationskoeffizient
Variation Coefficient of the Method / Coeficiente de variación del procedimiento
40,0
35,0
30,0
25,0
20,0
15,0
10,0
5,0
0,0
0,0 5,0 10,0 15,0 20,0 25,0 30,0
Sollwert / Target value / Valor nominal (mg/l)
Qualitätskontrolle
Quality control / Control de calidad

5.2 Quality Control for the User

Checking the
working equip-
ment
Pipette
Checking the
handling
operations
Influence of the sample
16
The certificate for the test kit, available for each lot produced, docu-
ments the quality of the reagents contained in the test kit.
Calibration function:
n = 10
Sollwert
Messergebnis
Target value
Result / Resultado
Valor nominal
The calculated function must agree, within specified tolerances, with the
(Standard / Patrón)
(Standard / Patrón)
mg/l
mg/l
CSB/COD/DQO
CSB/COD/DQO
4,0 4,7
function electronically stored in the photometer.
8,0 8,7
12,0 11,8
16,0 16,4
20,0 19,9
24,0 24,1
Confidence interval:
28,0 28,4
32,0 32,4
36,0 36,3
40,0 40,2
Maximum deviation from the desired value over the entire mea suring
Sollwert Chargenwert
Target value Lot value
Valor nominal Valor del lote
1,00 ± 0,03 0,99
range; every measurement value can be affected by this deviation; this
0,4
1,410 ± 0,020 A 1,403 A
± 1,0 mg/l ± 0,8 mg/l
parameter is a measure for the accuracy.
± 0,3 mg/l
± 2,5 % ± 1,4%
Standard deviation for the procedure:
Measurement for the dispersion of the measurement values over the
entire measuring range, ex pressed in ± mg/l.
Coefficient of variation for the procedure:
Measurement for the dispersion of the measurement values over the
35,0 40,0
entire measuring range, ex pressed in %. The smaller the standard devia-
Merck KGaA
tion/ coefficient of variation for the procedure, the more pronounced the
Laborleiter / Head of Lab.
Jefe de laboratorio
linearity of the calibration curve.
A complete check comprises the entire system, i. e. the working equipment
and the mode of operation. The photometer offers an optimum degree of
support in this re gard, in the form of the different quality mode. The instru-
ment, or the whole system (includ ing reagents and all accessories) will be
checked, depending on which quality mode selected. All of checking oper-
ations can thus be supported by the pho tometer and the check values
accordingly docu mented as per GLP (Good Laboratory Practice) recom-
mendations (see Function description, "Analytical Quality Assurance").
The following diagram provides an overview regarding internal quality-
assurance aspects:
Test kit Photometer
Carefully pipette 3.0 ml
Suspend the bottom Heat the reaction cell in
of the sample into a
sediment in the cell by the thermoreactor at
reaction cell, close tight-
swirling. 148 °C for 2 hours.
ly with the screw cap,
and mix vigorously.
Caution, the cell
becomes very hot!
Test for recovery
Thermoreactor
Remove the reaction Swirl the cell after
cell from the thermo- 10 minutes.
reactor and place in a
test-tube rack to cool.
= Test for the
overall system
Release 01/2012