Leica M125 C User Manual
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Leica M125 C User Manual

M series
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Summary of Contents for Leica M125 C

  • Page 1 Leica M125 C Leica M165 C Leica M205 C Leica M205 A User Manual...

  • Page 2: General Instructions

    Use in clean rooms Servicing Before using your microscope for the first The Leica M series can be used in clean rooms Repairs may only be carried out by Leica time, please read the "Safety concept" booklet without any problems.

  • Page 3: Safety Concept

    Safety Concept The individual modules of the Leica M stereo You can combine individual system articles microscopy series include an interactive with articles from external suppliers (e.g. cold CD-ROM with all relevant user manuals in 20 light sources, etc.). Please read the user manual other languages.

  • Page 4: Symbols Used

    Figures Hazards to personnel (1) Numbers in brackets within the descrip- Functional disturbances or damaged tions relate to the figures and the items instruments within those figures. Leica M Series User Manual...

  • Page 5: Safety Instructions

    Refer to "Safety Concept" booklet tion of Leica stereo microscopes of the M series. Place of use Ensure that: Intended use Refer to "Safety Concept" booklet The M series stereo microscopes and Refer to "Safety Concept"...
  • Page 6 The ergonomic design and construction of EC Declaration of Conformity the Leica M stereo microscopy series are Refer to "Safety Concept" booklet Transport intended to reduce the exertion of the user Use the original packaging for shipping or to a minimum.
  • Page 7 UV filters are installed in the observation Do not select a white, strongly reflective beam paths to protect the eyes. background for the specimen. The stray-light protection on the lamp housing prevents irradiation of the hands. Leica M Series User Manual...
  • Page 8 (follow the manufacturer's specifications and the minute meter on the supply unit). To minimize the risk of explosion, replace a discolored mercury lamp promptly. Leica Microsystems assumes no liability for damage caused by exploding, incor- rectly installed or improperly used mercury lamps.
  • Page 9 Leica LED5000 RL: Installing Optional Accessories Leica LED5000 RL: Use Assembly Leica LED5000 NVI: Assembly on High-Performance Stereo Base and Focusing Column Microscopes (Leica M125 C / Leica M165 C / Leica M205 C) Optics Carrier Leica LED5000 NVI: Installing Optional Accessories Tube Eyepieces...
  • Page 10 Transmitted-light Base Leica TL BFDF: Controls Eyepieces Transmitted-light Base Leica TL BFDF: Operation Magnification Factors of the Eyepieces Leica TL RC / Leica TL RCI: Controls Health Notes Leica TL RCI: The Deflection Mirror Dioptric Correction Leica TL RCI: Color Intensity and Temperature...
  • Page 11 Contents (Continued) Dimensional Drawings Leica M125 C / Leica M165 C Leica M205 C Leica LED5000 MCI Leica LED5000 RL Leica LED5000 NVI Specifications for the Bases Transmitted-light Base Leica TL ST Transmitted-light Base Leica TL BFDF Leica TL RC / Leica TL RCI...

  • Page 12: The Leica M Series

    The Leica M Series Leica M Series User Manual The Leica M Series...

  • Page 13: Congratulations

    It contains all the information you need regarding operation, safety and maintenance. Simply observing a few guidelines will ensure The M series embodies all the qualities you associate with the name Leica that even after years of intensive use, your stereo microscope will continue Microsystems: excellent objectives, high-quality engineering, and reli- to work as smoothly and reliably as on the very first day.

  • Page 14: A Step Towards Infinity

    – a sphere becomes an ellipse, a flat of movement and discover details in stereo surface curves toward the observer. microscopy that you could never see before. * Leica M205 C with 1.0× planapochromat and 10× eyepieces Leica M Series User Manual The Leica M Series...

  • Page 15: The Electronics: Comfort, Convenience And Safety For Your Experiments

    Never before have electronics been used as Reliability for your experiments diaphragm and more to the Leica LAS software. extensively in a Leica series as in the new Exact reproducibility of results is an increas- Thus you always know the conditions under M series.

  • Page 16: The Modular Design: Everything Is Relative

    The optics carriers, eyepieces, bases that cannot be met with standard parts, contact and more can be combined in any way you your Leica consultant. We have a solution for choose, allowing you to create the microscope every problem.

  • Page 17: Maximum Compatibility

    Maximum Compatibility Leica engineers were careful to ensure that the Tubes new M series – like its predecessors – remains The interface between the optics carrier and compatible with existing series. This means the tube has remained the same, so exist- that objectives, bases, tubes and so on can be ing tubes fit the new M series.
  • Page 18 On We Go If your new Leica microscope has already been If, on the other hand, you are assembling the assembled and commissioned by your Leica microscope yourself, continue with the "Assem- consultant, click here to skip through the instal- bly"...

  • Page 19: Assembly

    Assembly Leica M Series User Manual Assembly...

  • Page 20: Base And Focusing Column

    1. Securely install the column adapter on the 2. Securely screw the focusing column to the column using the four included screws. base using the six included screws. Tools used Hex socket screwdriver, 3 mm Leica M Series User Manual Assembly...

  • Page 21: Optics Carrier

    2. Press the optics carrier backwards to the column so that the screw fit into the thread focusing column and screw it in place using provided and the lug fits into the groove. your other hand. Leica M Series User Manual Assembly...

  • Page 22: Tube

    Tools used positioning screw meshes with the guide position. No tools required. groove. Preparations 1. Unscrew the positioning screw and remove the protective cover. Leica M Series User Manual Assembly...

  • Page 23: Eyepieces

    fit tightly and accurately. You can extend the overall magnification range 2. Remove the plastic tube guard. using available 10×, 16×, 25× and 40× wide- field eyepieces for persons wearing glasses. 4. Securely tighten the clamping screws. Leica M Series User Manual Assembly...

  • Page 24: Objective

    1. Remove the protective cap on the optics which is very heavy. Remove all specimens from on page 27. carrier by turning it. the stage plate first. 2. Screw the objective clockwise into the optics carrier. Leica M Series User Manual Assembly...

  • Page 25: Objective Nosepiece – Assembly

    3. Unscrew the three Phillips screws on the objective mount of the optics carrier and remove the intermediate ring. You can now adjust the parfocality (see instruc- tions on the next page). Leica M Series User Manual Assembly...

  • Page 26: Objective Nosepiece – Adjusting Parfocality

    5. Toggle to the 2× objective. 6. Select the strongest magnification and Preparation refocus until the specimen appears abso- Open the iris diaphragm. lutely sharp. Set the dioptric correction of the eyepieces to "0". Leica M Series User Manual Assembly...

  • Page 27: Ax Carrier – Preparation

    Before the AX carrier is installed, it may first switch in position. have to be prepared for the optics carrier to be used (Leica M125 C, M165 C, and M205 C). 4. Important: push the switch all the way into 1. Check the switch position.

  • Page 28: Ax Carrier – Assembly

    1. Move the slide of the AX carrier into mid position. 3. Screw the objective to the AX carrier. 5. Install the optics carrier on the AX carrier. Wrong Right Leica M Series User Manual Assembly...

  • Page 29: Transmitted-Light Base Leica Tl St

    Transmitted-light Base Leica TL ST Unpacking the base Focusing drive and column 2. Attach your focusing drive column to the The base is delivered with the adapter plate 1. Unscrew the extension plate from the base bottom using the 4 Allen screws.

  • Page 30: Transmitted-Light Base Leica Tl Bfdf: Before First Use

    Transmitted-light Base Leica TL BFDF: Before First Use Removing the transport anchors Before you can use the transmitted- light base for the first time, it is abso- lutely necessary to remove the two transport Anchor of mirror anchors as follows.

  • Page 31: Transmitted-Light Base Leica Tl Bfdf

    Transmitted-light Base Leica TL BFDF Standard delivery The selected stage is mounted on the base Standard stage The base is delivered with the adapter plate before commissioning. You can switch between 1. Take the glass plate from the rectangular installed. The selected stage (IsoPro cross-stage the stages at any time with just a few hand gap in the standard stage.

  • Page 32: Leica Tl Rc / Leica Tl Rci

    Leica TL RC / Leica TL RCI The base is delivered with the adapter plate Standard stage installed. The selected stage (IsoPro cross-stage 1. Take the glass plate from the rectangular or standard stage 10 450 562), and the focusing gap in the standard stage.

  • Page 33: Leica Isopro Manual Cross-Stage: Assembly

    Leica IsoPro Manual Cross-stage: Assembly IsoPro Cross-stage Before the IsoPro cross-stage is mounted to the Left or right operation Before the IsoPro cross-stage is mounted to the base, the axis containing the control buttons is If the controls are to be mounted on the left-...
  • Page 34 Leica IsoPro Manual Cross-stage: Assembly (Continued) 1. Take the glass plate from the cross-stage Control assembly Cross-stage assembly and turn it around. 1. Take the glass plate from the cross-stage 1. Place the cross-stage on the base. and turn it around.
  • Page 35 Leica IsoPro Manual Cross-stage: Assembly (Continued) 3. Screw the cross-stage evenly onto the three Focusing drive and column threaded holes. 1. Unscrew the extension plate from the base using the Allen key provided. 4. Push the cross-stage all the way back towards the column.

  • Page 36: Leica Isopro Motorized Cross-Stage: Assembly

    Leica IsoPro Motorized Cross-stage: Assembly Basics 1. Unpack the cross-stage from the transport 2. Secure the motorized cross-stage to the The transmitted light bases of the TL series packaging and position it on the transmit- base using 3 M4 screws.
  • Page 37 Leica IsoPro Motorized Cross-stage: Assembly (Continued) 3. Remove the two M3 screws and the holder 4. Remove the two M4 screws and the sleeve 5. Remove the four shock-absorbing cartons from the cross-stage. from the cross-stage. from the cross-stage.  ...
  • Page 38 Leica IsoPro Motorized Cross-stage: Assembly (Continued) The bases Cross-stage and base The TL BFDF, TL RC and RCI transmitted light Never move the sledge of the motor- bases can be equipped with three different ized cross-stage manually in the X...
  • Page 39 2. Plug the CTL2 plug of the Leica PSC control- ler into another CTL2 interface. 4. Connect the PC and X-Y stage DCI module (with a suitable USB cable).

  • Page 40: Cables: Terminals

    Cables: Terminals The new Leica M series features extensive The terminals The connection to the PC and to other instru- encoding with which various microscope data ments is made using the terminals on the rear and settings can be read out, transferred to the side of the column: PC and reproduced later.

  • Page 41: Cables: Cable Duct

    Tip: Estimate the length of the cable ends you 2. Remove the cover of the cable duct. will need before screwing on the cover. For thick cables, it is difficult to change the length retroactively. Leica M Series User Manual Assembly...

  • Page 42: Cables: Diagram

    Cables: Diagram Alternative installation LED5000 SLI Please use the Leica Application Suite to configure the functions of the system. LED5000 RL LED5000 MCI DCI Controlbox for IsoPro motorized stage TL RCI Power/USB 115/230 V PC USB Footswitch(es) Handwheel Smart Move...

  • Page 43: Leica Led5000 Mci

    3. Plug the other end of the cable into one of the three "CTL2" sockets on the column. Constraints The Leica LED5000 MCI cannot be used together with the objective nosepiece. Leica M Series User Manual Assembly...

  • Page 44: Leica Led5000 Mci: Alternative Assembly

    For such purposes, the Leica LED5000 MCI is directly fastened to the column. Installation on the column 1. Pull the retaining stirrup out of the Leica LED5000 MCI. Leica M Series User Manual Assembly...

  • Page 45: Leica Led5000 Rl: Assembly

    50 mm and 80 mm. Constraints The Leica LED5000 RL can be used only in 2. Place the Leica LED5000 RL against the Continued on next page. conjunction with the planapochromat 1× and objective from below, push it up as far as it planapochromat 0.63×...
  • Page 46 For optimum accessibility of the speci- column without integrated electronics, men, the ring illuminator should be the Leica LED5000 RL must be supplied with installed with the cable facing backwards. power via an external power supply unit (not However, it is also possible to turn the ring included in the delivery package).

  • Page 47: Leica Led5000 Rl: Installing Optional Accessories

    Leica LED5000 RL: Installing Optional Accessories Installing optional accessories You can screw the optional polarization set into place firmly using the metal plate provided. The optional diffuser is attached and screwed in below the ring illuminator. The optional polarization set (polarizer and analyzer) is attached and screwed in below the ring illuminator.

  • Page 48: Leica Led5000 Rl: Use

    Leica LED5000 RL: Use   The light of the Leica LED5000 RL can Using the front keypad Using the side keypad be very bright. Therefore, always switch Use the keys to toggle between on the illuminator before you look through the the illuminator segments.
  • Page 49 Leica LED5000 RL: Use (Continued) If you press for approx. 2 seconds, the segments rotate automatically until you press one of these keys again. If you change the segment using the key, the automatic changeover is kept. Pressing briefly stops the changeover.

  • Page 50: Leica Led5000 Nvi: Assembly On High-Performance Stereo Microscopes (Leica M125 C / Leica M165 C / Leica M205 C)

    Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes (Leica M125 C / Leica M165 C / Leica M205 C) Assembly of the Leica LED5000 NVI 2. Place the Leica LED5000 NVI on the focus- Press the Leica LED5000 NVI backwards to (10 450 659) ing column so that the screw fits into the...
  • Page 51 Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes (Leica M125 C / Leica M165 C / Leica M205 C) (Continued) 4. Unscrew the three Phillips screws on the 5. Set the optics carrier on the adapter of the 6. Install the remaining microscope compo-...
  • Page 52 Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes (Leica M125 C / Leica M165 C / Leica M205 C) (Continued) 8. Screw objective Leica Lists of recommended objectives that can be LED5000 NVI from below. used with the Leica LED5000 NVI without addi-...

  • Page 53: Leica Led5000 Nvi: Installing Optional Accessories

    Leica LED5000 NVI: Installing Optional Accessories Polarization set 2. The analyzer is installed between the tube The Leica LED5000 NVI can be equipped with and optics carrier. To do so, remove the tube an optional polarization set. Using the polariza- from the optics carrier.
  • Page 54 Leica LED5000 NVI: Installing Optional Accessories (Continued) Filter inserts 1. To disassemble the filter holder, push the The Leica LED5000 NVI is supplied with a filter holder approx. 1 cm to the side and filter holder as a standard feature. Commer- remove the holder downwards out of the cially available filters of size 1/2“...

  • Page 55: Quick Start Guide

    Quick Start Guide Leica M Series User Manual Quick Start Guide...

  • Page 56: The Fastest Route To Success

    The Fastest Route to Success Your Leica stereo microscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. Therefore, your next step should be to study the Quick Start Guide, which outlines the most important steps at a glance.

  • Page 57: Overview Of An M Series Microscope

    (or accessories) 5. Interchangeable objective 6. Adjustable eyepiece tubes 7. Eyepieces for spectacle wearers with dioptric correction and eyecups 8. Fastening screws for the eyepieces 9 . Trinocular tube 10. Built-in iris diaphragm Leica M Series User Manual Quick Start Guide...

  • Page 58: The Correct Interpupillary Distance

    Not to worry – after a little while, it will become automatic. Reference value The distance between eye and eyepiece measures approx. 22 mm for 10/23B wide-field eyepieces for persons wearing glasses. Leica M Series User Manual Quick Start Guide...

  • Page 59: Using The Eyepieces

    2. If an eyepiece is equipped with the inte- great. Each eyepiece offers a certain magnification factor that has a determinative effect on the total magnification. Furthermore, all Leica eyepieces can be equipped with practical grati- cules that enable measuring and quantifying of specimens.

  • Page 60: Focusing

    The coarse/fine adjustment carries a The focusing drive can be operated either focusing. load of up to 15 kg. left- or right-handed. The resolution of the coarse/fine adjust- ment is 1 μm. Leica M Series User Manual Quick Start Guide...

  • Page 61: Adjusting The Resistance Of The Focus Drive

    1. Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focus- ing. Leica M Series User Manual Quick Start Guide...

  • Page 62: Changing The Magnification (Zoom)

    The name indicates the zoom range 1. Look into the eyepieces. covered: 2. Focus on the specimen. Leica M125 C = 12.5:1 Leica M165 C = 16.5:1 3. Rotate the magnification changer until the Leica M205 C = 20.5:1 desired magnification is configured.

  • Page 63: Ratchet Steps And Magnification Levels

    Leica M Series User Manual Quick Start Guide...

  • Page 64: Parfocality: More Comfort And Convenience For Your Work

    Parfocality: More Comfort and Convenience for Your Work All Leica stereo microscopes are parfocally Parfocality matched, meaning that you can view a focused 1. Enlarge the view to the maximum level. specimen from the lowest to the highest magnification without having to refocus. There 2.

  • Page 65: Iris Diaphragm

    field. The and the depth of field increases. brighter, but the depth of field decreases. "depth of field" is the area of a specimen that is brought into sharp focus. Leica M Series User Manual Quick Start Guide...

  • Page 66: Eyepieces

    Eyepieces Leica M Series User Manual Eyepieces...

  • Page 67: Magnification Factors Of The Eyepieces

    Order number 10× ± 5 diopter settings 10 450 630 16× ± 5 diopter settings 10 450 631 25× ± 5 diopter settings 10 450 632 40× ± 5 diopter settings 10 450 633 Leica M Series User Manual Eyepieces...

  • Page 68: Health Notes

    The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separately. Please contact your Leica partner. Separate eyecups are an effective way of preventing infections. Leica M Series User Manual Eyepieces...

  • Page 69: Dioptric Correction

    Dioptric Correction   All Leica eyepieces are also available with built- Using the dioptric correction Note that when using dioptric correction, in dioptric correction, allowing the microscope 1. Set the dioptric correction of both the advantage of parfocality is lost – thus to be used without glasses even by those with eyepieces to the mid position ("0"...

  • Page 70: Dioptric Correction And Parfocality

    Dioptric Correction and Parfocality Leica stereo microscopes are parfocally Adjusting 7. Look into the eyepieces. matched. The prerequisite for this is the correct 1. Set the dioptric correction for both setting of the diopters and the parfocality. The eyepieces to "0".

  • Page 71: Graticules

    Use with the AX carrier   If possible, measure with the micro- scope carrier AX in vertical position. The measurements are more accurate without the convergence angle in the stereoscopic image. Leica M Series User Manual Eyepieces...
  • Page 72 Photography & Video Leica M Series User Manual Photography & Video...

  • Page 73: Photography & Video

    Leica cameras are capturing images, it lets you control the micro- with the motorized IsoPro cross-stage, even exactly right for you. Together with the Leica scope, illuminator, stages, cameras and more. images of large specimens can be captured...

  • Page 74: Photo Tubes And C-Mounts

    In addition to Leica cameras with the standard- image on the image sensor of the camera. ized C-mount interfaces, you can connect third- The Leica digital cameras are detailed in a sepa- party cameras to the microscope using a T2 rate user manual along with instructions for Usually, the ideal is for the digital camera to bayonet adapter.
  • Page 75 50 % of the light is diverted to the video/ photo beam path. Assembly Fasten the "trinocular tube 50 %" to the optics carrier instead of the binocular observation tube (refer to page 2 ). Leica M Series User Manual Photography & Video...
  • Page 76 Fasten the "trinocular tube 100 %" to the optics You can now photograph the specimen. carrier instead of the binocular observation tube (refer to page 22). Leica M Series User Manual Photography & Video...

  • Page 77: Microscope Carrier

    Microscope Carrier Leica M Series User Manual Microscope Carrier...

  • Page 78: The Microscope Carrier Ax

    Compatibility The planachromatic and planapochromatic objectives fit into the thread in the microscope carrier AX for the Leica M125 C, Leica 165 C and the Leica M205 C. 2. Push the microscope into the home posi- tion (mid position) to obtain a spatial view and tighten the clamping screw.
  • Page 79 Coaxial light and the Leica M205 C When using coaxial incident light together with 1. Unscrew the clamping screw. the Leica M205 C, unscrew the clamping screw and press the optics carrier towards the right 2. Push the microscope towards the left as (caution: the movement is only 2 mm).

  • Page 80: The Objective Nosepiece

      Older Leica objectives can continue to be used, but without parfocality during the objective change. Leica M Series User Manual Microscope Carrier...

  • Page 81: Objectives And Optical Accessories

    Objectives and Optical Accessories Leica M Series User Manual Objectives and Optical Accessories...

  • Page 82: The Different Types Of Objectives

    With planapochromatic objectives, the finest structures are visible with high contrast. The sophisticated apochromatic correction allows these objectives to attain the highest color brilliance and fidelity. Leica M Series User Manual Objectives and Optical Accessories...

  • Page 83: Leica M Series User Manual

    Bases Leica M Series User Manual Bases...

  • Page 84: Transmitted-Light Base Leica Tl St: Controls

    Transmitted-light Base Leica TL ST: Controls 1. Adapter plate for easy assembly of focusing Extension plate of the transmitted-light base TL ST Rear side of the transmitted-light base TL ST drives 1. Screws for changing the halogen lamp 2. Removable glass plate 2.

  • Page 85: Transmitted-Light Base Leica Tl St: Operation

    Transmitted-light Base Leica TL ST: Operation Light intensity control Transmitted-light control Bright field The left control adjusts the intensity of the The transmitted-light base TL BFDF has a slider Bright field is suitable for examining translu- 12 V/20 W halogen illumination.

  • Page 86: Transmitted-Light Base Leica Tl St: Lamp Replacement

    Transmitted-light Base Leica TL ST: Lamp Replacement Changing the halogen lamp Changing bulbs Precautionary measures   Before you change the lamp, it is abso- 1. Unscrew the two screws on the cooling unit When inserting the lamp, ensure that lutely necessary to unplug the power...

  • Page 87: Transmitted-Light Base Leica Tl Bfdf: Controls

    Transmitted-light Base Leica TL BFDF: Controls Adapter at the focusing drive 1. Adapter plate for easy assembly of focusing Extension plate of the transmitted-light base TL BFDF drives 2. Standard stage 10 450 562 3. Button to toggle between bright field and dark field...

  • Page 88: Transmitted-Light Base Leica Tl Bfdf: Operation

    Transmitted-light Base Leica TL BFDF: Operation Light intensity control Bright field Dark field Please observe the user manual – in Bright field is suitable for examining translu- In dark-field illumination, a ring illuminator is particular, all safety regulations – from cent objects featuring contrasting structures.

  • Page 89: Leica Tl Rc / Leica Tl Rci: Controls

    Leica TL RC / Leica TL RCI: Controls 3 4 5 Vertical column – transmitted-light base adapter plate 1. Power switch 1. Heat sink of the integrated halogen illumina- 2. Power connection socket tion (only TL RCI) 3. USB socket, type B 2.

  • Page 90: Leica Tl Rci: The Deflection Mirror

    Leica TL RCI: The Deflection Mirror Tilted mirror Functions of the rotary knob The built-in mirror features one flat and one The rotary knob (1) fulfills the following tasks: concave side and can be rotated and moved. The concave side has been specially designed Turning the deflection mirror from the flat...

  • Page 91: Leica Tl Rci: Color Intensity And Temperature

    The transmitted-light base TL RCI has two elec- Using a USB mouse (only TL RCI) tronic potentiometers that control the color The Leica USB mouse controls the CCIC and intensity (1) and color temperature (2). dimming function of the TL RCI base. Connect...

  • Page 92: Leica Tl Rc / Leica Tl Rci: Operation

    Leica TL RC / Leica TL RCI: Operation Setting the relief contrast Depending on the flap position, a part of Increased contrast without relief is attained The two switches on the left side of the trans- the opening of the built-in Fresnel lenses are if both diaphragms are set to 45°.

  • Page 93: Leica Tl Rci: Methods In Transmitted Light

    Leica TL RCI: Methods in Transmitted Light Vertical bright-field illumination Inclined transmitted light Single-sided dark field Suitable for stained amplitude specimens with Suitable for semitransparent, opaque speci- Suitable for fixed specimens and fine structures. sufficient contrast. mens such as foraminifera and fish eggs. Move the deflection mirror until the desired data are...

  • Page 94: Leica Tl Rci: Relief Images

    Leica TL RCI: Relief Images Starting position Positive relief contrast Negative relief contrast Suitable for semitransparent and transparent Suitable for semitransparent and transparent 1. Push the deflection mirror all the way back specimens. The phase structures look like hills. specimens. These settings result in a negative towards the column.
  • Page 95 Leica TL RCI: Relief Images (Continued) Dynamic relief contrast Constraints Suitable for semitransparent and transparent The relief methods provide good results from specimens. mid-zoom to high magnifications and with 1×, 1.6× and 2× magnifications. In the lower zoom By tilting the deflection mirror slightly, you can half and with weaker specimens, the object move the gaps over the entire field of view and...

  • Page 96: Using Filters

    3. Insert the desired filter. customer request, the filters are also available as one-off items. 1. Switch off the light source or click (TL RCI) the button for the shutter. 4. Switch the light source back on. Leica M Series User Manual Bases...

  • Page 97: Leica Isopro (Non-Motorized): Controls

    Leica IsoPro (Non-motorized): Controls Operating the cross-stage IsoPro 1. To move the stage in X direction, rotate the outer knob. 2. To move the stage in Y direction, rotate the inner control ring. Leica M Series User Manual Bases...

  • Page 98: Leica Isopro (Motorized): Controls

    Leica IsoPro (Motorized): Controls IsoPro X-Y stage DCI module Leica PSC controller 1. Sub-D interface for Leica SmartMove 1. Quick control/memory function 2. 3 CTL2 interfaces 2. Fine control in X direction 3. USB interface (type B) 3. Fine control in Y direction 1.

  • Page 99: Accessories

    Accessories Leica M Series User Manual Accessories...

  • Page 100: Leica Hand Wheel And Foot Switch

    Leica Hand Wheel and Foot Switch Up to five foot switches can be configured with a microscope system. They can be programmed in LAS or LAS X to control focus, illumination, and individual memory positions. The hand wheel is designed to control the motor focus.

  • Page 101: Leica Smarttouch

    The most important control functions on the settings, please refer to the Leica SmartTouch control unit can be adjusted to your specific manual or the Leica Application Suite help file. needs with freely programmable rotary knobs and function buttons. Controls The functions of the individual knobs and screen elements can be configured both...

  • Page 102: Leica Psc Controller

    file. Connection Connect the Leica PSC controller and the Leica IsoPro motorized cross-stage. Fine control of the cross-stage The motorized Leica IsoPro cross-stage offers an accuracy of up to 0.25μm. To move to a position with maximum accuracy, use the left and right knobs on the joystick.

  • Page 103: Dimensional Drawings

    Dimensional Drawings Leica M Series User Manual Dimensional Drawings...

  • Page 104: Leica M125 C / Leica M165 C

    Leica M125 C / Leica M165 C Leica M125 C / Leica M165 C with incident light base and new focusing column (dimensions in mm) Leica M Series User Manual Dimensional Drawings...
  • Page 105 Leica M125 C / Leica M165 C (Continued) Leica M125 C / Leica M165 C with transmitted-light base TL ST and manual focus (dimensions in mm) Leica M Series User Manual Dimensional Drawings...
  • Page 106 Leica M125 C / Leica M165 C (Continued) Leica M125 C / Leica M165 C with transmitted-light stand TL RC and manual focus (dimensions in mm) Leica M Series User Manual Dimensional Drawings...
  • Page 107 Leica M125 C / Leica M165 C (Continued) Leica M125 C / Leica M165 C with transmitted-light stand TL RC, manual cross-stage Leica IsoPro and manual focus (dimensions in mm) max. 370 max. 370 X = +-75 mm Leica M Series User Manual...

  • Page 108: Leica M205 C

    Leica M205 C Leica M205 C with transmitted-light stand TL ST and manual focusing drive (dimensions in mm) Leica M Series User Manual Dimensional Drawings...
  • Page 109 Leica M205 C (Continued) Leica M205 C with transmitted-light stand TL RCI and manual focusing drive (dimensions in mm) Leica M Series User Manual Dimensional Drawings...
  • Page 110 Leica M205 C (Continued) Dimensions of Leica M205 C with transmitted-light stand TL RCI, manual cross-stage Leica IsoPro and manual focus (dimensions in mm) max. 410 X = +-75 mm Leica M Series User Manual Dimensional Drawings...

  • Page 111: Leica Led5000 Mci

    Leica LED5000 MCI Leica LED5000 MCI (dimensions in mm) 275.3 Leica M Series User Manual Dimensional Drawings...

  • Page 112: Leica Led5000 Rl

    Leica LED5000 RL Leica LED5000 RL (dimensions in mm) 58 mm Leica M Series User Manual Dimensional Drawings...

  • Page 113: Leica Led5000 Nvi

    Leica LED5000 NVI Leica LED5000 NVI for high-performance stereo microscopes (dimensions in mm) Leica M Series User Manual Dimensional Drawings...
  • Page 114 Specifications for the Bases Leica M Series User Manual Specifications for the Bases...

  • Page 115: Transmitted-Light Base Leica Tl St

    Transmitted-light Base Leica TL ST Light source Halogen lamp 12 V/20 W Quick illuminant change Illuminated area 50 mm Power supply unit Input voltage 100–240 V~, frequency 50–60 Hz energy consumption 30 W max., ambient temperature 10–40 °C Connections Power plug, power switch Weight 7.4 kg...

  • Page 116: Transmitted-Light Base Leica Tl Bfdf

    Matching of high num. aperture Yes** Remote control options Yes*** AntiShock Pads Dimensions (W×H×D) 340×390×90 mm *With cold light source Leica CLS150 LS **Concave mirror *** With external light source Leica M Series User Manual Specifications for the Bases...

  • Page 117: Leica Tl Rc / Leica Tl Rci

    Yes*** Remote control options Yes**** AntiShock Pads Dimensions of base (W×H×D) 340×390×95 mm 340×440×95 mm * Single-sided **With cold light source Leica CLS150 LS ***Concave mirror **** With external light source Leica M Series User Manual Specifications for the Bases...

  • Page 118: Leica Isopro Motorized Xy Stage

    Leica IsoPro Motorized XY Stage Compatibility Leica incident light base and TL bases (TL BFDF, TL RC, TL RCI) Stage size (L × W × H) 335.5 mm × 370 mm × 41.5 mm Travel 152 mm × 102 mm (6" × 4")

  • Page 119: Appendix

    Appendix Leica M Series User Manual Appendix...

  • Page 120: Calculating The Total Magnification And Field Of View Diameter

    Calculation example: field of view diameter in the specimen MTOT VIS = MO × ME × z × q × r N FOV M O × z ×q ×r 1 × 25 × 4 × 1.5 × 1.25 = 187.5× Leica M Series User Manual Appendix...

  • Page 121: Care, Maintenance, Contact Persons

    (Switzerland) AG, CH-9435 Heerbrugg. away from the instruments. decades, your Leica microscope will continue to work as well as on the very first day. Plugs, optical systems and mechanical parts E-mail contact: must not be disassembled or replaced, Warranty benefits...
  • Page 122 Keep accessories in a dust-free place when Remove dust with a pneumatic rubber bulb not in use. or with a soft brush. Clean objectives and eyepieces with special optic cleaning cloths and with pure alcohol. Leica M Series User Manual Appendix...
  • Page 123 CONNECT WITH US! Leica Microsystems (Schweiz) AG · Max-Schmidheiny-Strasse 201 · 9435 Heerbrugg, Switzerland T +41 71 726 34 34 · F +41 71 726 34 44 www.leica-microsystems.com...

This manual is also suitable for:

M165 cM205 cM205 a

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