Leica M User Manual
  1. Manuals
  2. Brands
  3. Leica Manuals
  4. Microscope
  5. M
  6. User manual

Leica M User Manual

M stereomicroscopes
Hide thumbs Also See for M:
Table of Contents

Advertisement

Quick Links

See also: Instruction Manual
Leica M Stereo-
microscopes
User Manual

Advertisement

Table of Contents
loading

Related Manuals for Leica M

Summary of Contents for Leica M

  • Page 1 Leica M Stereo- microscopes User Manual...

  • Page 2: User Manual

    Should you have any questions, please consult your local Leica representative. You will find the address of the closest local representative as well as valuable informa- tion about products and services from Leica Microsystems on our homepage at www.leica-microsystems.com...

  • Page 3: Table Of Contents

    Binocular tubes, optical accessories ..... . 35 Objective nosepiece (MZ16 and MZ16 A) ....36 Leica M Stereomicroscopes User Manual...
  • Page 4 Dimensions ..........67 Leica M Stereomicroscopes User Manual...

  • Page 5: Safety Concept

    – There are two connection sockets for ∅ 4 mm grounding cables in the base of the swinging-arm stand. The Leica M series can be used in clean rooms without any problems and Use in Clean Rooms they can be cleaned as described on page 60. Please observe the following rules: –...

  • Page 6: Safety Regulations

    (frequently changing activities). – detailed introduction of the personnel while considering ergonomic and organizational work-related aspects. The ergonomic optics concept and the design of the Leica M series aim at limiting the strain on the user to the lowest possi- ble level.
  • Page 7 Opening the instrument Only authorized Leica personnel are permitted to repair electri- cal equipment. Before opening up the equipment, pull out the power cable. If you touch the live interior of the equipment, you may receive an electric shock.

  • Page 8: Symbols

    Integration in The following must be observed if Leica products are build into third-party products third-party products: The manufacturer of the complete system or the person putting it on the market is responsible for adhering to applicable safety regulations, laws and guidelines.

  • Page 9: Controls And Functions

    11 Built-in double-iris diaphragm Holds the binocular tube (or accessory) (MZ16/MZ16 A only) to the optics carrier. • The special functions of the Leica MZ16 A are 6 Interchangeable objective shown in the user manual M2-116-0. Achromatic, planachromatic or planapochromatic...
  • Page 10 Leica MZ7 Leica MZ9 Leica design by Ernest Igl/Christophe Apothéloz Leica MZ12 Leica MZ16 Leica M Stereomicroscopes – Overview...

  • Page 11: Use

    • Engage the ratchets whenever magnifications need to be exactly reproducible. For MZ6, MZ9 , MZ12 MZ16: Press the switch. For MZ7 : Turn the knurled ring counterclockwise. For setting ratchets at the MZ16 A, see the user manual M2-116-0. Leica M Stereomicroscopes – Operation...
  • Page 12 Magnification with When the MZ12 /MZ16 planachromatic and planapochromatic MZ12 /MZ16 objectives are used on the Leica MS5, MZ6, MZ7 and MZ9 planachromatic and the magnification is increased by the factor 1.25× . This factor planapochromatic is taken into account in the tables.

  • Page 13: Ergonomics

    If you have problems with the viewing configuration on your stereomicroscope, ask your Leica consultant for the most ergonomic Leica solutions. Ergo objective With the achromatic Ergo objective 0.4×– 0.63× for the Leica MS5, MZ6, MZ7 and MZ9 it is possible to focus in the region of 90mm (63.5 mm –...

  • Page 14: Interpupillary Distance

    Motor focus Frequently repeated fine-motor tasks, such as focusing by hand, can lead to muscle fatigue and hand problems. Using the Leica motor focusing system, every equipment can be moved up and down without applying force. Interpupillary distance, eye contact...

  • Page 15: Eye Contact, Eyecups

    • The focusing drive can be operated from either the right or the left side. • When focusing on a new object, always start with the lowest magnification, because the larger field of view makes the object easier to find. Afterwards, select the desired magnifi- cation. Leica M Stereomicroscopes – Operation...

  • Page 16: Focusing

    Using the rotary knob, bring the object into focus. Coarse/fine drive The coarse/fine drive supports up to 15 kg. – Resolution with 5 kg load: 1 µm – Resolution with 10 kg load: 2 µm Focus approximately with the larger knob. Fine-focus with the smaller (concentric) knob.

  • Page 17: Microscope Carrier: Stereoscopic And Axial Observation

    • The optics carrier cannot be turned sideways in the micro- scope carrier AX. Stereoscopic A three-dimensional image is required for examining plastic observation objects and for working. Shift the stereomicroscope to the central position. Leica M Stereomicroscopes – Operation...
  • Page 18 Otherwise the field of view is underex- posed (vignetting). – In the position for axial observation through the right or left beam path, turn the aperture clockwise until it stops. Leica M Stereomicroscopes – Operation...

  • Page 19: Diopter Settings

    Adjusting diopter settings and parfocality Tips for your work Leica stereomicroscopes are parfocally matched. This means that, once you have focused on a feature in the manner de- scribed, taking account of diopter settings, you can zoom the magnification up and down without refocusing. You only need to refocus if you want to look at higher or lower planes in the object.
  • Page 20 9. Engage the highest magnification parfocality position. 10. Refocus if necessary. 11. Slowly move the magnification changer from the lowest magnification to the highest. • The image sharpness must remain constant (parfocal). Otherwise, repeat the procedure. Leica M Stereomicroscopes – Operation...

  • Page 21: Assembly

    2b Side-faced column with drive housing, 300 mm or 500 mm Microscope carrier Optics carrier Interchangeable objective Binocular tube Wide-field eyepiece for spectacle wearers, with eyecups Various ErgoModules™ or accessories for video, photography, drawing, fluorescence, coaxial incident light etc. Leica M Stereomicroscopes – Assembly...

  • Page 22: Stand

    Primary voltage: 115 /-230 V, 50/60 Hz Secondary voltage: 7 V 28 VA Fuses, primary For 115 V: 315 m AT / for 230 V: 160 m AT Switch positions Position I: Transmitted light 6 V/20 W Position II: External incident lamp 20 W...
  • Page 23 Fit the enclosed two 315 mAt fuses in the fuse holder instead of the two 160 mAT fuses (see the section "Changing fuses" on the right). Connect the power cable to the socket of the base and to the power source. Leica M Stereomicroscopes – Assembly...
  • Page 24 Inclined transmitted light which traverses the object obliquely transmitted light will produce effects advantageous for observing small translu- cent objects such as foraminifera and fish eggs. Move the lever slowly towards the column of the stand until the desired effect is obtained. Leica M Stereomicroscopes – Assembly...

  • Page 25: Transmitted-Light Stand, Bright And Dark Field

    In this case, the light beams penetrate the object at a flat angle. Fine detail structures and contours contrast brilliantly and brightly from dark background. Slide the handle out to the right: dark field. Leica M Stereomicroscopes – Assembly...

  • Page 26: Transmitted-Light Stand Hl

    (TVC, active ∅ 9 mm). Insert light guide into the base at the rear. Connect light guide to the light source. Accessory condenser For stereomicroscopes with high apertures such as Leica ➔ Basis MZ12 , MZ16 and MZ16 A with objective 1.6×, the resolution can be increased by inserting an accessory condenser.

  • Page 27: Swinging-Arm Stand Esd

    Retighten the clamping screw. Optics carrier ➜ Fit the optics carrier in the microscope carrier microscope carrier. Turn the optics carrier sideways in the microscope carrier as required. Retighten the clamping screw. Leica M Stereomicroscopes – Assembly...
  • Page 28 Reposition the safety ring (see page 28). Sideways movement Release the clamping screw. Move the instrument sideways on the cross-member. Retighten the clamping screw. Altering the overhang Release the clamping screw. Displace the horizontal arm. Retighten the clamping screw. Leica M Stereomicroscopes – Assembly...

  • Page 29: Large Swinging-Arm And Table-Clamp Stands

    Slide the drive housing with side-faced carrier rod onto the horizontal arm. Retighten the clamping lever. The clamping levers can be positioned as required. Pull out the clamping lever along its axis and turn it. Leica M Stereomicroscopes – Assembly...
  • Page 30 Pull out or push in the horizontal arm. Retighten the clamping screw. During the sideways movement, observe the limit stops (see page 30). Altering the overhang Release the clamping screw. Displace the horizontal arm. Retighten the clamping screw. Leica M Stereomicroscopes – Assembly...

  • Page 31: Optics Carrier, Optical Accessories

    /MZ16 if a spacer ring is fitted (10 446 172). • When the MZ12 /MZ16 planachromatic and planapo- chromatic objectives are used on the Leica MS5, MZ6, MZ7 and MZ9 , the magnification is increased by the factor 1.25×. 10 447 103 MZ16 A...
  • Page 32 CA Compatible if used with intermediate ring (order separately) Intermediate ring is recommended M Magnification increased by factor 1.25× or higher Remove intermediate ring (10 446 393); already included with MZ9 (C) Large fields of view at low magnifications are not fully illuminated.

  • Page 33: Microscope Carrier

    Use the screw to secure the optics carrier in the position required. Only for MZ7 Apply the adhesive Remove the screws. scale of Remove the rotary knobs. magnifications Apply the adhesive scales. Replace the rotary knobs. Leica M Stereomicroscopes – Assembly...

  • Page 34: Binocular Tubes, Optical Accessories

    1× planachromatic objective. When the spacer ring has been removed by applying the tool provided, the larger-diameter planachromatic and planapo- chromatic objectives for the MZ12 /MZ16 can be used. Leica M Stereomicroscopes – Assembly...

  • Page 35: Objective Nosepiece (Mz16 And Mz16 A)

    Preparation Remove the securing strap from the objective nosepiece: Remove both screws (at the bottom). Leica MZ16 and MZ16 A are delivered with a threaded adapter for objectives. Remove the adapter to use the objective nosepiece. Invert the optics carrier.
  • Page 36 2× planapochromatic as well as the 1× planapochromatic objective without refocusing. Otherwise turn the objective at the ring nut until the object is focused. Fasten the ring nut with both screws. Do not overtighten both screws. Leica M Stereomicroscopes – Assembly...

  • Page 37: Illuminators

    Illuminators Transformers Follow the safety instructions given on page 7. Leica transformers may only be opened by authorized service personnel, and the power cable must be disconnected before- hand. The transformers are not to be used outdoors. Regulating transformer The regulating transformer 0–7 V/40 VA is used to operate the 6 V/10 W and 6 V/20 W lamps.
  • Page 38 Set the voltage selector on the rear side to 115 V or 230 V. Attach the power cable to the power supply. Connect the lamp. Adjust the brightness with the three- step switch (4/5/6 V). Leica M Stereomicroscopes – Illuminations...

  • Page 39: Incident Lamp 6 V/10 W

    Attach the lamp to the connector on lampholder arm the lampholder arm. Fit the filter holder, complete with KG1 heat-absorbing filter, to one of the pegs. • A total of 4 filter holders with ∅ 50 mm filters can be attached. Leica M Stereomicroscopes – Illuminations...
  • Page 40 Turn the socket to the separation position. Pull the socket forcibly out of the housing. Hold the new bulb in a cloth and push it into position. Replace the socket in the housing. The socket clicks into position. Leica M Stereomicroscopes – Illuminations...

  • Page 41: Incident Lamp 6 V/20 W

    • A total of 4 filter holders with ∅ 32 mm filters can Diffusing and be attached. other filters A heat-absorbing filter is built in. Push the filter holder, complete with diffusing filter, onto one of the pegs, and swing it in. Leica M Stereomicroscopes – Illuminations...
  • Page 42 Align the light spot with the object. Retighten the clamping screw. Adjust the size of the light spot by moving the condenser lens forward or back. If necessary, swing in a filter (page 42). Leica M Stereomicroscopes – Illuminations...
  • Page 43 Retighten the clamping screw. Using the slotted screw, bring the two filament images into focus. Displace the filament images so that they are opposite to one another, but in contact. Swing back the diffusing filter. Leica M Stereomicroscopes – Illuminations...

  • Page 44: Cold Light Sources

    The antistatic Leica L2 cold-light source is powerful, small, compact, inexpensive and suitable for simple observation tasks with the Leica MS5 and MZ6 stereo microscopes. In addition to one or two-arm light guides, corresponding accessories are al- so available for the coaxial, vertical and transmitted-light illumi- nation methods.

  • Page 45: Fiber-Optic Light Guide

    Cold light source Leica CLS series The Leica CLS series is a high-performance line for high light intensity within the smallest possible space and flicker-free white light with the lowest possible thermal effect on the ob- jects. The comprehensive line of accessories enables unlimited use.

  • Page 46: Supply (Line) Lamp 25W

    Using the clamping screw, secure the quarter-wave plate to the objective mount. Using the knurled ring, turn the quarter-wave plate until the required effect is obtained. Please observe the notes on the use of the aperture in the AX microscope carrier (p. 19). Leica M Stereomicroscopes – Illuminations...

  • Page 47: Near-Vertical Illuminator

    • Shadowing by tools is eliminated. • This illuminator can only be used with the achromatic objec- tives having focal lengths of 100, 150, 175 and 200 mm. • Spacer rings are required for the Leica MZ7 , MZ9 , MZ12 to secure the near-vertical illuminator to the optics carrier (page 32).
  • Page 48 To switch on the cold-light illuminator, refer to the user manual provided with it. No light? Turn the rotary knob. • A movable prism directs the light from the left or right outlet onto the object. Leica M Stereomicroscopes – Illuminations...

  • Page 49: Accessories

    Set the lever to OPEN: The double-iris diaphragm (aperture diaphragm) is open. The depth of field increases as the lever is moved from OPEN to 1. Remember, however, that – brightness decreases, so exposure times become longer – resolution deteriorates. Leica M Stereomicroscopes – Accessories...

  • Page 50: Drawing Tube, Discussion Tube

    Discussion tube At the discussion tube two observers see the same erect, laterally-correct, three-dimensional image. The assembly and use of the discussion tube are described in the separate user manual M2-263-0. Leica M Stereomicroscopes – Accessories...

  • Page 51: Video/Phototubes

    10 445 924 50% 10 446 229 100% Leica MZ16, MZ16 A HD50 10 446 197 10 445 931 10 445 925 Leica MS5, MZ6, MZ7 , MZ9 MZ12 , MZ FLIII 10 445 927 Leica M Stereomicroscopes – Accessories...
  • Page 52 • Video/phototubes are designed to support modular photomicrography systems, and Leica digital, video, film or SLR cameras. • The modular photomicrographic systems and the Leica digi- tal cameras are detailed in a separate user manual, along with instructions for their assembly and use.
  • Page 53 Fit the 1× video/photo objective. Example: With this equipment you can, for example, attach a digital or video camera. Fit the video objective (2) on a Leica DC digital camera (3) with the C-mount connection. Connect the video objective with the mounted Leica DC digital camera on the right output of the video/phototube.
  • Page 54 Example: With this equipment you can, for example, attach a digital or video camera. Fit the video objective (2) on a Leica DC digital camera (3) with the C-mount connection. Connect the video objective with the mounted Leica DC digital camera on the right output of the video/phototube.

  • Page 55: Attachment For Vertical And Oblique Observation

    • The graticules for length measurements and numbering are fitted in mounts and can be inserted into the eyepieces. • A special measuring graticule is available for the Leica MZ16 A (see the user manual M2-116). • A high-precision object micrometer with 50-mm scale, 0.1 mm and 0.01 mm graduation, allows for calibration...

  • Page 56: Stages, Polarization

    ∅ 120 mm port. Place the cup stage on it. Thermo stages The Leica MATS thermocontrol system is a heating system for microscopes and stereomicroscopes. Leica MATS allows the observation of temperature-sensitive specimens and living cells in biology, medicine and pharmaceutics under exact tempera- ture conditions.

  • Page 57: Tips And Hints: What To Do If

    Color photographs Remedy: have a yellow cast. • Use artificial-light film. • Turn the regulating knob of the transformer clockwise to the position of maximum voltage (bright field transmitted-light stand, page 24; transformer, page 38). Leica M Stereomicroscopes – Notes...

  • Page 58: Care And Maintenance

    Our instruments are known for that! If, contrary to expectations, your stereomi- croscope develops a fault, please call in a specialist – contact your local Leica agent or Leica Microsystems (Switzerland) Ltd, CH-9435 Heerbrugg. Protect your •...
  • Page 59 You can clean polymers harmlessly • With warm soapy water, wiping down afterwards with distilled water. • With ethanol (industrial alcohol) and isopropanol. When cleaning with ethanol or isopropanol, adhere to the appropriate safety regulations. Leica M Stereomicroscopes – Notes...

  • Page 60: Of View Diameter

    Factor 1.25× Magnification down binocular tube: 1 × 25 × 4 × 1.5 × 1.25 = 187.5 × M TOT VIS = M O × M E × z × q × r Diameter of field of view: N FOV ∅...

  • Page 61: Optical Data For Ms5, Mz6

    Optical data of Leica MS5, MZ6 1× Plan 1× Planapo* 1.6× Planapo* 0.63× Planapo* 0.5× Plan* 0.32× Achromat 0.5× Achromat 1.5× Achromat Objectives 1× Achromat 2× Achromat 0.8× Achromat 0.63× Achromat 0.8× Plan* Working distance (mm) 81 Plan 55 Planapo...
  • Page 62 25.5 16.5 14.4 31.8 20.6 11.5 39.8 25.7 32.9 63.7 41.2 0.63 14.9 10.4 20.4 11.8 13.2 18.2 25.5 16.5 14.5 1.25 31.8 20.6 11.7 40×/6B 40.8 26.3 32.9 63.7 41.2 81.5 52.7 101.9 65.8 Leica M Stereomicroscopes User Manual...

  • Page 63: Mz9

    95.5 40×/6B 119.4 62.5 31.3 149.3 102.4 238.8 62.5 298.5 358.2 * Position 6 for MZ9 only ** When using the planachromatic and planapochromatic objectives MZ12 , the magnification is increased by the factor 1.25×. Leica M Stereomicroscopes User Manual...
  • Page 64 32.9 63.7 41.2 79.6 51.4 95.5 61.7 0.63 14.9 10.4 20.4 11.8 13.2 18.2 25.5 16.5 14.5 1.25 31.8 20.6 11.7 40.8 26.3 40×/6B 32.9 63.7 41.2 81.5 52.7 101.9 65.8 127.4 82.3 152.9 98.8 Leica M Stereomicroscopes User Manual...

  • Page 65: Mz16, Mz16 A

    13.4 22.7 10.5 45.4 56.8 20.5 11.7 25.6 51.2 25.6 1.25 51.2 102.4 51.2 40×/6B 81.9 102.4 204.8 102.4 161.3 201.6 403.2 204.8 11.5 289.8 * Zoom positions 0.71 and 11.5 only for MZ16/MZ16 A Leica M Stereomicroscopes User Manual...

  • Page 66: Dimensions

    Dimensions of Leica MS5 with incident-light stand with transmitted-light stand 1.0x Achromat 1× Achromat 1× Achromat Measurements in mm Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency. Leica M Stereomicroscopes User Manual...
  • Page 67 Dimensions of Leica MZ6 with incident-light stand with transmitted-light stand 1.0x Achromat 1× Achromat 1× Achromat Measurements in mm Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency. Leica M Stereomicroscopes User Manual...
  • Page 68 Dimensions of Leica MZ7 with incident-light stand with transmitted-light stand 1× Plan 1.0x Plan 1.0x Plan 1× Plan Measurements in mm Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency. Leica M Stereomicroscopes User Manual...
  • Page 69 Dimensions of Leica MZ9 with incident-light stand with transmitted-light stand 1× Plan 1.0x Plan 1.0x Plan 1× Plan Measurements in mm Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency. Leica M Stereomicroscopes User Manual...
  • Page 70 Dimensions of Leica MZ12 with incident-light stand with transmitted-light stand 1× Plan 1.0x Plan 1.0x Plan 1× Plan Measurements in mm MZ12 MZ12 Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency. Leica M Stereomicroscopes User Manual...
  • Page 71 Dimensions of Leica MZ16 with incident-light stand with transmitted-light stand 1x Planapo 1x Planapo Measurements in mm MZ16 MZ16 Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency. Leica M Stereomicroscopes User Manual...
  • Page 72 Dimensions of Leica MZ16 A with incident-light stand with transmitted-light stand Measurements in mm Additional dimensional diagrams involving Leica ErgoModule™ are available from your Leica agency. Leica M Stereomicroscopes User Manual...

This manual is also suitable for:

Ms5Mz6Mz75Mz95Mz125Mz16 ... Show all

Table of Contents