Use in clean rooms Servicing Before using your microscope for the first The Leica M series can be used in clean rooms Repairs may only be carried out by Leica time, please read the "Safety concept" booklet without any problems.
Safety Concept The individual modules of the Leica M stereo You can combine individual system articles microscopy series include an interactive with articles from external suppliers (e.g. cold CD-ROM with all relevant user manuals in 20 light sources, etc.). Please read the user manual other languages.
(1) Numbers in brackets within the descrip- tant information that, if not observed, tions relate to the figures and the items can cause the following: within those figures. Hazards to personnel Functional disturbances or damaged instruments Leica M Series User Manual...
Refer to "Safety Concept" booklet tion of Leica stereo microscopes of the M series. Place of use Ensure that: Intended use Refer to "Safety Concept" booklet The M series stereo microscopes and Refer to "Safety Concept"...
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Use the original packaging for shipping or Thorough training of the personnel, giving transporting the individual modules of the consideration to ergonomic and organiza- Leica M stereo microscopy series and the tional aspects. accessory components. The ergonomic design and construction of...
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– Safety – Part one: general Technical safety requirements To connect the Leica M Series microscopes to The device must be disposed of in power, use a power cable that meets the power accordance with 2012/19/EU WEEE Radio interference suppression in compli- cable specifications in your country.
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The microscope must be connected to a grounded outlet. Always position the microscope so that you can disconnect it from the power supply at any time. The power cable is provided as the power disconnect device. Leica M Series User Manual...
Leica LED5000 RL: Use On We Go Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes (Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A) Assembly Leica LED5000 NVI: Installing Optional Accessories Base and Focusing Column...
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Leica MATS TPX Microscope Carrier Specifications The Microscope Carrier AX Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A The Objective Nosepiece Transmitted-light Base Leica TL BFDF Objectives and Optical Accessories Motorized Focus Drives With "Zoom" Carrier...
It contains all the information you need regarding operation, safety and maintenance. Simply observing a few guidelines will ensure The M series embodies all the qualities you associate with the name Leica that even after years of intensive use, your stereo microscope will continue Microsystems: excellent objectives, high-quality engineering, and reli- to work as smoothly and reliably as on the very first day.
– a sphere becomes an ellipse, a flat of movement and discover details in stereo surface curves toward the observer. microscopy that you could never see before. * Leica M205 C with 1.0× planapochromat and 10× eyepieces Leica M Series User Manual...
The Electronics: Comfort, Convenience and Safety for Your Experiments Never before have electronics been used as Reliability for your experiments diaphragm and more to the Leica LAS X soft- extensively in a Leica series as in the new Exact reproducibility of results is an increas- ware.
The optics carriers, eyepieces, bases that cannot be met with standard parts, contact and more can be combined in any way you your Leica consultant. We have a solution for choose, allowing you to create the microscope every problem.
Maximum Compatibility Leica engineers were careful to ensure that the Tubes new M series – like its predecessors – remains The interface between the optics carrier and compatible with existing series. This means the tube has remained the same, so exist- that objectives, bases, tubes and so on can be ing tubes fit the new M series.
On We Go If your new Leica microscope has already been If, on the other hand, you are assembling assembled and commissioned by your Leica the microscope yourself, continue with the consultant, click here to skip through the instal- "Assembly" chapter, which begins on page 17.
1. Securely install the column adapter on the 2. Securely screw the focusing column to the column using the four included screws. base using the six included screws. Tools used Hex socket screwdriver, 3 mm Leica M Series User Manual Assembly...
1. Securely install the column adapter on the 2. Securely screw the focusing column to the column using the four included screws. base using the six included screws. Tools used Hex socket screwdriver, 3 mm Leica M Series User Manual Assembly...
1. Place the focusing column on the side. drive. directly on the base; no extension plate is required. Tools used Hex socket screwdriver, 3 mm 2. Insert the four screws provided into the outer holes of the base. Leica M Series User Manual Assembly...
Tools used positioning screw meshes with the guide position. No tools required. groove. Preparations 1. Unscrew the positioning screw and remove the protective cover. Leica M Series User Manual Assembly...
You can extend the overall magnification range 2. Remove the plastic tube guard. using available 10×, 16×, 25× and 40× wide- field eyepieces for persons wearing glasses. 4. Securely tighten the clamping screws. Leica M Series User Manual Assembly...
1. Remove the protective cap on the optics which is very heavy. Remove all specimens from on page 27. carrier by turning it. the stage plate first. 2. Screw the objective clockwise into the optics carrier. Leica M Series User Manual Assembly...
3. Unscrew the three Phillips screws on the objective mount of the optics carrier and remove the intermediate ring. You can now adjust the parfocality (see instruc- tions on the next page). Leica M Series User Manual Assembly...
5. Toggle to the 2× objective. 6. Select the strongest magnification and Preparation refocus until the specimen appears abso- Open the iris diaphragm. lutely sharp. Set the dioptric correction of the eyepieces to "0". Leica M Series User Manual Assembly...
Before the AX carrier is installed, it may first switch in position. have to be prepared for the optics carrier to be used (Leica M125 C, M165 C, and M205 C / M205 A). 4. Important: push the switch all the way into the mount while reinserting the screws.
1. Move the slide of the AX carrier into mid position. 3. Screw the objective to the AX carrier. 5. Install the optics carrier on the AX carrier. Wrong Right Leica M Series User Manual Assembly...
Transmitted-light Base Leica TL BFDF: Before First Use Removing the transport anchors • Before you can use the transmitted- light base for the first time, it is abso- lutely necessary to remove the two transport Anchor of mirror anchors as follows.
Transmitted-light Base Leica TL BFDF Standard delivery The selected stage is mounted on the base Standard stage The base is delivered with the adapter plate before commissioning. You can switch between 1. Take the glass plate from the rectangular installed. The selected stage (XY stage or the stages at any time with just a few hand gap in the standard stage.
Leica IsoPro Manual XY Stage: Assembly IsoPro XY stage Before the IsoPro XY stage is mounted to the Left or right operation Before the IsoPro XY stage is mounted to the base, the axis containing the control buttons is If the controls are to be mounted on the...
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Leica IsoPro Manual XY Stage: Assembly (Continued) 1. Take the glass plate from the XY stage and Control assembly XY stage assembly turn it around. 1. Take the glass plate from the XY stage and 1. Place the XY stage on the base.
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Leica IsoPro Manual XY Stage: Assembly (Continued) 3. Screw the XY stage evenly onto the three Focusing drive and column threaded holes. 1. Unscrew the extension plate from the base using the Allen key provided. 4. Push the XY stage all the way back towards the column.
Cables: Connections The new Leica M series features extensive The terminals The connection to the PC and to other instru- encoding with which various microscope data ments is made using the terminals on the rear and settings can be read out, transferred to the side of the column: PC and reproduced later.
Tip: Estimate the length of the cable ends you 2. Remove the cover of the cable duct. will need before screwing on the cover. For thick cables, it is difficult to change the length retroactively. Leica M Series User Manual Assembly...
Cables: Diagram Alternative installation LED5000 SLI Please use the Leica Application Suite X to configure the functions of the system. LED5000 RL LED5000 MCI PC USB Power/USB 115/230 V Footswitch(es) Handwheel SmartTouch Leica M Series User Manual Assembly...
3. Plug the other end of the cable into one of the three "CTL2" sockets on the column. Constraints The Leica LED5000 MCI cannot be used together with the objective nosepiece. Leica M Series User Manual Assembly...
For such purposes, the Leica LED5000 MCI is directly fastened to the column. Installation on the column 1. Pull the retaining stirrup out of the Leica LED5000 MCI. Leica M Series User Manual Assembly...
50 mm and 80 mm. Constraints The Leica LED5000 RL can be used only in 2. Place the Leica LED5000 RL against the Continued on next page. conjunction with the planapochromat 1× and objective from below, push it up as far as it planapochromat 0.63×...
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For optimum accessibility of the speci- column without integrated electronics, men, the ring illuminator should be the Leica LED5000 RL must be supplied with installed with the cable facing backwards. power via an external power supply unit (not However, it is also possible to turn the ring included in the delivery package).
Leica LED5000 RL: Installing Optional Accessories Installing optional accessories You can screw the optional polarization set into place firmly using the metal plate provided. The optional diffuser is attached and screwed in below the ring illuminator. The optional polarization set (polarizer and analyzer) is attached and screwed in below the ring illuminator.
Leica LED5000 RL: Use The light of the Leica LED5000 RL can Using the front keypad Using the side keypad be very bright. Therefore, always switch Use the keys to toggle between on the illuminator before you look through the the illuminator segments.
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Leica LED5000 RL: Use (Continued) If you press for approx. 2 seconds, the segments rotate automatically until you press one of these keys again. If you change the segment using the key, the automatic changeover is kept. Pressing briefly stops the changeover.
Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes (Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A) Assembly of the Leica LED5000 NVI 2. Place the Leica LED5000 NVI on the focus- Press the Leica LED5000 NVI backwards to...
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Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes (Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A) (Continued) 5. Set the optics carrier on the adapter of the 6. Install the remaining microscope compo- 4.
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Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes (Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A) (Continued) 8. Screw objective Leica Lists of recommended objectives that can be LED5000 NVI from below. used with the Leica LED5000 NVI without addi-...
Leica LED5000 NVI: Installing Optional Accessories Polarization set 2. The analyzer is installed between the tube The Leica LED5000 NVI can be equipped with and optics carrier. To do so, remove the tube an optional polarization set. Using the polariza- from the optics carrier.
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Leica LED5000 NVI: Installing Optional Accessories (Continued) Filter inserts 1. To disassemble the filter holder, push the The Leica LED5000 NVI is supplied with a filter holder approx. 1 cm to the side and filter holder as a standard feature. Commer- remove the holder downwards out of the cially available filters of size 1/2“...
The Fastest Route to Success Your Leica stereo microscope has been delivered in completely assembled condition by your Leica partner, and naturally you want to get right to work. Therefore, your next step should be to study the Quick Start Guide, which outlines the most important steps at a glance.
Not to worry – after a little while, it will become automatic. Reference value The distance between eye and eyepiece measures approx. 22 mm for 10/23B wide-field eyepieces for persons wearing glasses. Leica M Series User Manual Quick Start Guide...
Each eyepiece offers a certain magnification grated dioptric correction, turn the value to factor that has a determinative effect on the the "0" mark. total magnification. Furthermore, all Leica eyepieces can be equipped with practical grati- cules that enable measuring and quantifying of specimens.
The resolution of the coarse/fine adjust- The focusing drive can be operated either focusing. ment is 1 µm. left- or right-handed. • The coarse/fine adjustment carries a load of up to 15 kg. Leica M Series User Manual Quick Start Guide...
1. Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing. Leica M Series User Manual Quick Start Guide...
The name indicates the zoom range 1. Look into the eyepieces. covered: 2. Focus on the specimen. Leica M125 C = 12.5:1 Leica M165 C = 16.5:1 3. Rotate the magnification changer until the Leica M205 C = 20.5:1 desired magnification is configured.
2. Push the bottom button upwards to enable the position of the magnification changer and enabled, photographs, measurement results the ratchet steps. the eyepiece and objective combination used. etc. can be reproduced more accurately. Leica M Series User Manual Quick Start Guide...
Parfocality: More Comfort and Convenience for Your Work All Leica stereo microscopes are parfocally Parfocality matched, meaning that you can view a focused 1. Enlarge the view to the maximum level. specimen from the lowest to the highest magnification without having to refocus. There 2.
The and the depth of field increases. brighter, but the depth of field decreases. "depth of field" is the area of a specimen that is brought into sharp focus. Leica M Series User Manual Quick Start Guide...
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Eyepieces Leica M Series User Manual Eyepieces...
The risk can be kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be ordered separately. Please contact your Leica partner. Separate eyecups are an effective way of preventing infections. Leica M Series User Manual Eyepieces...
Dioptric Correction All Leica eyepieces are also available with built- Using the dioptric correction Note that when using dioptric correction, in dioptric correction, allowing the microscope 1. Set the dioptric correction of both the advantage of parfocality is lost – thus to be used without glasses even by those with eyepieces to the mid position ("0"...
Dioptric Correction and Parfocality Leica stereo microscopes are parfocally Adjusting 7. Look into the eyepieces. matched. The prerequisite for this is the correct 1. Set the dioptric correction for both setting of the diopters and the parfocality. The eyepieces to "0".
Use with the AX carrier If possible, measure with the micro- scope carrier AX in vertical position. The measurements are more accurate without the convergence angle in the stereoscopic image. Leica M Series User Manual Eyepieces...
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Photography & Video Leica M Series User Manual Photography & Video...
Leica Application Suite X tation has become an invaluable part of their However, if you require absolute control The "Leica Application Suite X", or "LAS X" for work. Research results can be presented in an over the camera and need the capability for short, is, as it were, the digital extension of the attractive manner;...
T2 connection. However, these third- turn, the C-mount adapter for the respective field of vision and concentrates on the center of party cameras are not integrated into the Leica trinocular tube is connected to this interface. the field of vision.
50 % of the light is diverted to the video/ photo beam path. Assembly Fasten the "trinocular tube 50 %" to the optics carrier instead of the binocular observation tube (refer to page 22). Leica M Series User Manual Photography & Video...
Fasten the "trinocular tube 100 %" to the optics You can now photograph the specimen. carrier instead of the binocular observation tube (refer to page 22). Leica M Series User Manual Photography & Video...
Assembly Before mounting the ErgoTube, please remove the transport lock screw under the tube. Other- wise the photo slider cannot be used. Leica M Series User Manual Photography & Video...
Compatibility The planachromatic and planapochromatic objectives fit into the thread in the microscope carrier AX for the Leica M125 C, Leica M165 C, Leica M205 C and Leica M205 A. 2. Push the microscope into the home posi- tion (mid position) to obtain a spatial view and tighten the clamping screw.
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Leica M205 A 1. Unscrew the clamping screw. When using coaxial incident light together with the Leica M205 C / Leica M205 A, unscrew the 2. Push the microscope towards the left as clamping screw and press the optics carrier...
With planapochromatic objectives, the finest structures are visible with high contrast. The sophisticated apochromatic correction allows these objectives to attain the highest color brilliance and fidelity. Leica M Series User Manual Objectives and Optical Accessories...
Transmitted-light Base Leica TL BFDF: Controls Button to toggle between bright field/dark field Extension plate of the transmitted-light base TL BFDF Connector for cold light sources (light conductor active f = 10 mm, end tube f = 13 mm) Leica M Series User Manual...
Turn the control as far as it will go towards the brightness. "DF" ("dark field"). Transmitted-light control The Leica TL BFDF transmitted-light base has a control that switches the light from "bright field" to "dark field". Fingertip with bright field illumination...
Leica IsoPro (Non-motorized): Controls Operating the XY stage IsoPro 1. To move the stage in X direction, rotate the outer knob. 2. To move the stage in Y direction, rotate the inner control ring. Leica M Series User Manual Bases...
Leica LED5000 MCI Contact with the base • The Leica LED5000 MCI (for "Multi Contrast If the optics carriers are accidentally Illumination") is a universal high-output illu- lowered too far, contact between the minator. Three groups of 3 LEDs each can be base and MCI may result.
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The bottom row primarily provides contrast. The Leica LED5000 MCI can also be controlled by the LAS X (Leica Application Suite X) software. Maximum brightness Maximum contrast...
Use the "+" and "–" keys to adjust the and off completely or in various combinations. brightness in 10 increments. Touch either The Leica LED5000 RL has been designed for It is controlled using either the integrated or via of the two buttons to adjust the intensity in use with the 1.0×...
Leica Hand Wheel and Foot Switch Up to five foot switches can be configured with a microscope system. They can be programmed in LAS X to control focus, illumination, and indi- vidual memory positions. The hand wheel is designed to control the motor focus.
Leica SmartTouch with a few hand movements and never lose manual or the Leica Application Suite X help sight of all your important optical parameters. file. The most important control functions on the...
Leica M125 C / Leica M165 C Leica M125 C / Leica M165 C with incident light base and new focusing column (dimensions in mm) Leica M Series User Manual Dimensional Drawings...
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Leica M125 C / Leica M165 C (Continued) 496.5 Leica M125 C / Leica M165 C with transmitted-light base TL3000 Ergo and manual XY stage (dimensions in mm) 496.5 Y = +-75 mm Leica M Series User Manual Dimensional Drawings...
Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A Leica M125 C Leica M165 C Leica M205 C / Leica M205 A Optical data Zoom 12.5 : 1 manual 16.5 : 1 manual 20.5 : 1 manual/motorized...
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Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A (Continued) Leica M125 C Leica M165 C Leica M205 C / Leica M205 A Optics carrier 100 % apochromatic optical system CMO (Common Main Objective) lead-free Ω/mm...
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Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A (Continued) Leica M125 C Leica M165 C Leica M205 C / Leica M205 A Accessories Standard objective (parfocal 1× planapochromatic in the objective nosepiece) Additional objectives (parfocal in the 2×...
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Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A (Continued) Modular System Stands, illuminators Transmitted-light base TL4000 BFDF: Bright and circular dark field TL3000 Ergo: Bright field, dark field, Rottermann Contrast TL5000 Ergo: Bright field, two-sided dark field, Rottermann Contrast...
Coated optics for increasing the color temperature Matching of high num. aperture Yes** Remote control options Yes*** AntiShock Pads Dimensions (W×H×D) 340×390×90 mm *With cold light source Leica CLS150 LS **Plan mirror ***With external light source Leica M Series User Manual Specifications...
Calculation example: field of view diameter in the specimen MTOT VIS = MO × ME × z × q × r N FOV ∅ OF: M O × z ×q ×r 1 × 25 × 4 × 1.5 × 1.25 = 187.5× Leica M Series User Manual Appendix...
(Switzerland) AG, CH-9435 Heerbrugg. away from the instruments. decades, your Leica microscope will continue to work as well as on the very first day. Plugs, optical systems and mechanical parts E-mail contact:...
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Keep accessories in a dust-free place when Remove dust with a pneumatic rubber bulb not in use. or with a soft brush. Clean objectives and eyepieces with special optic cleaning cloths and with pure alcohol. Leica M Series User Manual Appendix...
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CONNECT WITH US! Leica Microsystems (Schweiz) AG · Max-Schmidheiny-Strasse 201 · 9435 Heerbrugg, Switzerland T +41 71 726 34 34 · F +41 71 726 34 44 www.leica-microsystems.com...
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