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Hach DR 2800 Procedures Manual

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Catalog Number DOC022.53.00725

DR 2800 Spectrophotometer

PROCEDURES MANUAL

June 2007 Edition 2

Important Note:

All procedures are available on the DR 2700 with the following exceptions:

Chlorine Dioxide Method 8345, Direct Reading Method, MR

COD Method 8000, Reactor Digestion Method, ULR (0.7 to 40.0)

TNTplus procedures

© Hach Company, 2007. All rights reserved. Printed in Germany.

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Summary of Contents for Hach DR 2800

Page 1 All procedures are available on the DR 2700 with the following exceptions: Chlorine Dioxide Method 8345, Direct Reading Method, MR COD Method 8000, Reactor Digestion Method, ULR (0.7 to 40.0) TNTplus procedures © Hach Company, 2007. All rights reserved. Printed in Germany.
Page 2 Visit us at www.hach.com...
Page 3 Table of Contents Denotes USEPA accepted or approved for water or wastewater analysis. Method Precision Data ............................1 Working with Chemical Procedures ........................9 Alachlor Method 10202 Immunoassay Method Aluminum Method 8012 Aluminon Method Powder Pillows (0.008 to 0.800 mg/L) Aluminum Method 8326 Eriochrome Cyanine R Method Powder Pillows (0.002 to 0.250 mg/L Al...
Page 4 Table of Contents Denotes USEPA accepted or approved for water or wastewater analysis. Chlorine Dioxide Method 8065 Chlorophenol Red Method LR (0.01 to 1.00 mg/L) Chlorine Dioxide Amaranth Method (20 to 500 µg/L) Chlorine Dioxide Method 8345 Direct Reading Method MR (1–50 mg/L) Chlorine Dioxide ®...
Page 5 Table of Contents Denotes USEPA accepted or approved for water or wastewater analysis. Copper Method 8143 Porphyrin Method Powder Pillows LR (1 to 210 µg/L) Copper ® Method 8506 and Method 8026 Bicinchoninate Method Powder Pillows or AccuVac Ampuls (0.04 to 5.00 mg/L) Cyanide –...
Page 6 Table of Contents Denotes USEPA accepted or approved for water or wastewater analysis. Method 8033 Dithizone Method Powder Pillows (3 to 300 µg/L) Lead Method 10216 PAR Method TNTplus™ 850 (0.1 to 2.0 mg/L Pb) Manganese Method 8149 1-(2-Pyridylazo)-2-Naphthol PAN Method Powder Pillows LR (0.006 to 0.700 mg/L) Manganese Method 8034 Periodate Oxidation Method Powder Pillows...
Page 7 Table of Contents Denotes USEPA accepted or approved for water or wastewater analysis. Method 8155 Salicylate Method Powder Pillows (0.01 to 0.50 mg/L NH –N) Nitrogen, Ammonia Method 8038 Nessler Method (0.02 to 2.50 mg/L NH –N) Nitrogen, Ammonia ™ Method 10031 Salicylate Method Test ‘N Tube Vials HR (0.4 to 50.0 mg/L NH...
Page 8 Table of Contents Denotes USEPA accepted or approved for water or wastewater analysis. ® Method 8316 Indigo Carmine Method AccuVac Ampuls LR (6 to 800 µg/L O Oxygen Demand, Chemical Method 8000 Reactor Digestion Method TNTplus™ LR (TNT821, 3–150 COD); HR (TNT822, 20–1500mg/L COD) Oxygen Demand, Chemical Method 8000 Reactor Digestion Method...
Page 9 Table of Contents Denotes USEPA accepted or approved for water or wastewater analysis. (Orthophosphate) 3– Method 8114 Molybdovanadate Method Test ‘N Tube™ Vials HR (1.0 to 100.0 mg/L PO Phosphorus, Reactive 3– Method 10055 Ascorbic Acid Rapid Liquid Method Pour-Thru Cell LR (19 to 3000 µg/L PO Phosphorus, Reactive (Orthophosphate) ®...
Page 10 Table of Contents Denotes USEPA accepted or approved for water or wastewater analysis. Method 8120 Colorimetric Method Powder Pillows (0.005 to 0.700 mg/L) Sulfate ® Method 8051 SulfaVer 4 Method Powder Pillows or AccuVac Ampuls (2 to 70 mg/L) Sulfide Method 8131 Methylene Blue Method (5 to 800 µg/L) Sulfite...
Page 11 Method Precision Data Expected Precision for DR 2800 Methods 95% Confidence Interval Method Stored PARAMETER Method Concentration Range Lower Target Upper Number Program Limit Conc. Limit 8012 Aluminon 0.008–0.800 mg/L Al 0.385 0.393 0.402 Eriochrome 8326 0.002–0.250 mg/L Al 0.092 0.096...
Page 12 Method Precision Data Expected Precision for DR 2800 Methods (continued) 95% Confidence Interval Method Stored PARAMETER Method Concentration Range Lower Target Upper Number Program Limit Conc. Limit 8023 Diphenylcarbohydra 0.010–0.700 mg/L Cr 0.497 0.500 0.503 zide 8023 Diphenylcarbohydra 0.010–0.700 mg/L Cr 0.497...
Page 13 Method Precision Data Expected Precision for DR 2800 Methods (continued) 95% Confidence Interval Method Stored PARAMETER Method Concentration Range Lower Target Upper Number Program Limit Conc. Limit 8031 0.07–7.00 mg/L I 4.43 4.46 4.50 Iodine 8031 DPD, AccuVac 0.07–7.00 mg/L I 4.17...
Page 14 Method Precision Data Expected Precision for DR 2800 Methods (continued) 95% Confidence Interval Method Stored PARAMETER Method Concentration Range Lower Target Upper Number Program Limit Conc. Limit – 8039 0.3–30.0 mg/L NO –N 14.7 15.4 Cadmium Reduction HR, AccuVac –...
Page 15 Method Precision Data Expected Precision for DR 2800 Methods (continued) 95% Confidence Interval Method Stored PARAMETER Method Concentration Range Lower Target Upper Number Program Limit Conc. Limit 8000 200–15,000 mg/L COD Reactor Digestion 8000 3–150 mg/L COD Reactor Digestion 10067 Manganese III 20–1000 mg/L COD...
Page 16 Method Precision Data Expected Precision for DR 2800 Methods (continued) 95% Confidence Interval Method Stored PARAMETER Method Concentration Range Lower Target Upper Number Program Limit Conc. Limit 3– 8114 Molybdovanadate 0.3–45.0 mg/L PO 30.42 30.80 31.18 3– 8178 Amino Acid 0.23–30.00 mg/L PO...
Page 17 Method Precision Data Expected Precision for DR 2800 Methods (continued) 95% Confidence Interval Method Stored PARAMETER Method Concentration Range Lower Target Upper Number Program Limit Conc. Limit 5–750 mg/L Suspended Solids 8006 Photometric Susp. Solids Tannin & Lignin 8193 Tyrosine 0.1–9.0 mg/L tannin...
Page 18 Visit us at www.hach.com...
Page 19 Working with Chemical Procedures This document explains the layout and location of information in a typical written chemical procedure. Layout may vary slightly between procedures, depending on the level of detail and the steps required for a particular test. Procedure Method Name Approval of Acceptance of...
Page 20 Working with Chemical Procedures Levels of common sample substances or conditions that will cause inaccurate results Sulfide (5 to 800 μg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Strong reducing substances (sulfite, thiosulfate and Interferes by reducing the blue color or preventing its development.
Page 21 Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2005. All rights reserved. Printed in Germany. Updated June 05 Edition 1 Working with Chemical Procedures Page 11 ProceduresExplained.fm...
Page 22 Visit us at www.hach.com...
Page 23: Immunoassay Method
Alachlor Immunoassay Method Method 10202 Scope and Application: For water This test is semi-quantitative. Results are expressed as greater or less than the threshold value used. Test Preparation This method analyzes for Alachlor in water. Sample calibrators and reagents are added to cuvettes coated with Alachlor-specific antibodies.
Page 24 Alachlor Immunoassay for Water λ 1. Press 2. Insert Adapter A. 3. Label an Antibody 4. Insert the cuvettes into Cuvette for each calibrator the rack snugly. SINGLE WAVELENGTH and each sample to be λ Press and the OPTIONS tested. button.
Page 25 Alachlor 9. After 10 minutes mix 10. At the end of the 11. Wash each cuvette the contents of the rack for 20-minute period, discard forcefully and thoroughly 30 seconds using the the contents of all the four times with deionized technique described cuvettes into an water.
Page 26 Alachlor Measuring the Color 16. Label and fill a Zeroing 17. Insert the filled 18. Press 19. Insert the first ZERO Cuvette with deionized Zeroing Cuvette into the calibrator into the cell The display will show: water. Wipe the outside of cell holder—arrow pointing holder.
Page 27 Alachlor Loading the Rack—The cuvette rack is designed so that it may be inverted with the cuvettes in place. Identify each cuvette with a sample or calibrator number and insert all the cuvettes in the rack before beginning the procedure. Fit the cuvettes snugly into the rack, but do not force them or they may be difficult to remove and their contents may spill.
Page 28 Alachlor Sensitivity The Alachlor immunoassay test cannot differentiate between certain herbicides and metabolites, but it detects their presence to differing degrees. Table 2 shows the required concentration for selected chemicals. Table 2 Required Concentration for Selected Chemicals Concentration to give a positive Concentration to give a positive Compound response of 0.1 ppb Alachlor...
Page 29: Summary Of Method
Alachlor Summary of Method Immunoassay tests use antigen/antibody reactions to test for specific organic compounds in water and soil. Alachlor-specific antibodies, attached to the walls of plastic cuvettes, selectively bind and remove Alachlor from complex sample matrices. A prepared sample and a reagent containing enzyme-conjugate molecules (analyte molecules attached to molecules of an enzyme) are added to the Antibody Cuvettes.
Page 30 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 31: Powder Pillows
Aluminum Aluminon Method Method 8012 (0.008 to 0.800 mg/L) Powder Pillows Scope and Application: For water and wastewater Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: Digestion is required for determining total aluminum. Clean all glassware with 6.0 N HCl and deionized water before use to remove contaminants from the glass.
Page 32 Aluminum (0.008 to 0.800 mg/L) 5. Press 6. Invert repeatedly for 7. Blank Preparation: 8. Add one Bleaching 3 TIMER>OK one minute to dissolve the Pour 10 mL of the mixture Reagent Powder Pillow to powder. Undissolved into a square sample cell. the blank.
Page 33 Aluminum (0.008 to 0.800 mg/L) Interferences Interfering Substance Interference Levels and Treatments Greater than 300 mg/L as CaCO . Samples with greater than 300 mg/L acidity as CaCO must be treated as follows: Add one drop of m-Nitrophenol Indicator Solution to the sample taken in step 3.
Page 34: Accuracy Check
Aluminum (0.008 to 0.800 mg/L) Figure 1 Fluoride Interference Graph mg/L Al (Reading from instrument) True Aluminum Concentration Sample Collection, Storage, and Preservation Collect samples in a clean glass or plastic containers. Preserve the sample by adjusting the pH to 2 or less with nitric acid (about 1.5 mL per liter). Preserved samples can be stored up to six months at room temperature.
Page 35 Aluminum (0.008 to 0.800 mg/L) Standard Solution Method 1. Prepare a 0.4-mg/L aluminum standard solution as follows: Pipet 1.00 mL of Aluminum Standard Solution, 100-mg/L as Al , into a 250-mL volumetric flask. 2. Dilute to the mark with deionized water. Prepare this solution daily. Perform the aluminum procedure as described above.
Page 36 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 37: Stored Programs
Aluminum Eriochrome Cyanine R Method Method 8326 Powder Pillows (0.002 to 0.250 mg/L Al Scope and Application: For water Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: Clean all glassware with 6.0 N HCl and deionized water before use to remove contaminants from the glass. Check the sample temperature.
Page 38 Aluminum (0.002 to 0.250 mg/L Al 5. Press 6. After the timer expires, 7. Insert the stopper. 8. Blank Preparation: TIMER>OK add one Hexamethylene- Invert several times to Put one drop of ECR A 30-second reaction tetramine Buffer Reagent dissolve powder. Masking Reagent Solution period will begin.
Page 39 Aluminum (0.002 to 0.250 mg/L Al Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Acidity Greater than 62 mg/L as CaCO Alkalinity Greater than 750 mg/L as CaCO Greater than 1000 mg/L as CaCO – Greater than 1000 mg/L as CaCO 0.2 mg/L (error is –5% of reading) 2 mg/L (error is –5% of reading)
Page 40 Aluminum (0.002 to 0.250 mg/L Al e. Use a combination hot plate/stirrer to boil and stir the sample for at least 30 minutes. Add deionized water as needed to maintain a sample volume of 20-40 mL. Do not boil dry. Cool the solution to near room temperature.
Page 41 Aluminum (0.002 to 0.250 mg/L Al Sample Collection, Storage, and Preservation Collect samples in clean glass or plastic containers. Preserve samples by adjusting the pH to 2 or less with concentrated nitric acid* (about 1.5 mL per liter). Preserved samples can be stored up to six months at room temperature.
Page 42 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 43 Aluminum Chromazurol S Method Method 10215 TNTplus™ 848 (0.02 to 0.50 mg/L Al) Scope and Application: For drinking water, surface water, swimming pool water, wastewater, and process analysis. Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read the Safety Advice and Expiration Date on the package.
Page 44 Aluminum (0.02 to 0.50 mg/L Al) TNTplus Method 10215 1. Pipet 2.0 mL of 2. Pipet 3.0 mL of 3. Add one level spoonful 4. Cap and invert the vial Solution A into the vial. sample into the vial. of Reagent B to the vial. 2–3 times until no more streaks can be seen in the solution.
Page 45 Aluminum (0.02 to 0.50 mg/L Al) Sample Blanks If the Sample Blank value falls within the allowable range, this value will be used to correct the result automatically. The instrument will subtract the Sample Blank from the uncorrected result. Samples without color or turbidity do not require sample blanks. Interferences The ions listed in Table 1...
Page 46 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 47 Arsenic Silver Diethyldithiocarbamate Method Method 8013 (0 to 0.200 mg/L As) Scope and Application: For water, wastewater, and seawater; distillation is required; USEPA accepted for reporting for drinking and wastewater analysis (digestion required) Adapted from Standard Methods for the Examination of Water and Wastewater. Procedure is equivalent to USEPA Method 206.4 for wastewater and Standard Method 3500-As for drinking water analysis.
Page 48: User Programs
Arsenic (0 to 0.200 mg/L As) Silver Diethyldithiocarbamate Method 8013 Arsenic 1. Perform the User 2. To run the test, press 3. Select the test. 4. Prepare the Distillation Programming procedure Apparatus for arsenic USER PROGRAMS on page 5. Make note of recovery.
Page 49 Arsenic (0 to 0.200 mg/L As) 9. Using a graduated 10. Use a serological 11. Use a serological 12. Press TIMER>OK cylinder, add 25 mL of pipet to add 1 mL of pipet to add 3 mL of A 15-minute reaction Hydrochloric Acid, ACS, to Stannous Chloride Potassium Iodide Solution...
Page 50: Reagent Preparation
Arsenic (0 to 0.200 mg/L As) 21. Wipe the blank and 22. Press . The 23. Prepared Sample: 24. Wipe the prepared ZERO insert it into the cell holder display will show the Pour the reacted arsenic sample and insert it into with the fill line facing right.
Page 51: Program Options
Arsenic (0 to 0.200 mg/L As) Calibration Standard Preparation Perform a new calibration for each lot of arsenic absorber solution. 1. Prepare a 10.0-mg/L arsenic working standard by pipetting 10.0 mL of Arsenic Standard Solution, 1000 mg/L As into a 1000-mL volumetric flask. 2.
Page 52 Arsenic (0 to 0.200 mg/L As) 9. Press the arrow once to move to the next concentration. Insert the prepared DOWN sample in the cell holder. Press to accept the absorbance value. Repeat steps for READ each standard. 10. Press .
Page 53 Arsenic (0 to 0.200 mg/L As) Required Apparatus (continued) Description Quantity/Test Unit Cat. No. Flask, volumetric, Class A, 500-mL each 14574-49 Pipet Filler, safety bulb each 14651-00 Pipet, serological, 5-mL each 532-37 Pipet, volumetric, Class A, 1.00-mL each 14515-35 Pipet, volumetric, Class A, 2.00-mL each 14515-36 Pipet, volumetric, Class A, 4.00-mL...
Page 54 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 55: Immunoassay Method
Atrazine Immunoassay Method Method 10050 Scope and Application: For water This test is semi-quantitative. Results are expressed as greater or less than the threshold value used. Test Preparation This method analyzes for Atrazine in water. Sample calibrators and reagents are added to cuvettes coated with Atrazine-specific antibodies.
Page 56 Atrazine Immunoassay for Water 1. Press 2. Insert Adapter A. 3. Label an Antibody 4. Insert the cuvettes into Cuvette for each calibrator the rack snugly. SINGLE WAVELENGTH and each sample to be λ Press and the OPTIONS tested. button. Enter 450 nm and press 5.
Page 57 Atrazine 9. After 10 minutes mix 10. At the end of the 11. Wash each cuvette the contents of the rack for 20-minute period, discard forcefully and thoroughly 30 seconds (Using the the contents of all the four times with deionized 1-cm MicroCuvette Rack cuvettes into an water.
Page 58 Atrazine Measuring the Color 16. Label and fill a Zeroing 17. Insert the filled 18. Press 19. Insert the first ZERO Cuvette with deionized Zeroing Cuvette into the calibrator into the cell The display will show: water. Wipe the outside of cell holder—arrow pointing holder.
Page 59 Atrazine Loading the Rack—The cuvette rack is designed so that it may be inverted with the cuvettes in place. Identify each cuvette with a sample or calibrator number and insert all the cuvettes in the rack before beginning the procedure. Fit the cuvettes snugly into the rack, but do not force them or they may be difficult to remove and their contents may spill.
Page 60 Atrazine Sensitivity The Atrazine immunoassay test cannot differentiate between certain triazines and metabolites, but it detects their presence to differing degrees. Table 2 shows the required concentration for selected chemicals. Table 3 shows compounds not detectalbe at 10,000 ppb. Table 2 Required Concentrations for Selected Chemicals Compound Concentration to give a positive result at 3 ppb (in ppb) Ametryne...
Page 61 Atrazine Summary of Method Immunoassay tests use antigen/antibody reactions to test for specific organic compounds in water and soil. Atrazine-specific antibodies, attached to the walls of plastic cuvettes, selectively bind and remove Atrazine from complex sample matrices. A prepared sample and a reagent containing enzyme-conjugate molecules (analyte molecules attached to molecules of an enzyme) are added to the Antibody Cuvettes.
Page 62 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 63: Powder Pillows
Barium Turbidimetric Method Method 8014 (2 to 100 mg/L) Powder Pillows Scope and Application: For water, wastewater, oil-field water, and seawater Adapted from Snell and Snell, Colorimetric Methods of Analysis, Vol. II, 769 (1959). Test Preparation Before starting the test: Perform a standard curve adjustment or a new calibration for each new lot of reagent.
Page 64: Sample Collection, Preservation, And Storage
Barium (2 to 100 mg/L) 5. Press 6. Blank Preparation: 7. When the timer 8. Wipe and the TIMER>OK Fill another square sample expires, wipe the blank prepared sample and A five-minute reaction cell with 10 mL of sample. and insert the blank into insert the prepared period will begin.
Page 65: Accuracy Check
Barium (2 to 100 mg/L) To adjust the calibration curve using the reading obtained with the 90.0-mg/L standard solution: 1. Touch on the current program menu. Touch on the OPTIONS>MORE STANDARD ADJUST Options menu. 2. Touch . Touch to accept the displayed concentration. If an alternate ADJUST concentration is used, touch the number in the box to enter the actual concentration, then touch...
Page 66 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 67: Stored Programs
Benzotriazole/Tolyltriazole UV Photolysis Method Method 8079 Powder Pillows (Benzotriazole 1.0 to 16.0 mg/L, Tolyltriazole 1.0 to 20.0 mg/L) Scope and Application: For cooling or boiler water Adapted from Harp, D., Proceedings 45th International Water Conference, 299 (October 22–24, 1984) Test Preparation Before starting the test: Avoid fingerprints on the quartz surface of the lamp.
Page 68 Benzotriazole/Tolyltriazole (Benzotriazole 1.0 to 16.0 mg/L, Tolyltriazole 1.0 to 20.0 mg/L) 5. Put on UV safety 6. Insert the ultraviolet 7. Press 8. When the timer TIMER>OK goggles. lamp into the mixing bottle. expires, turn the lamp off. A 5-minute reaction period Remove the lamp from the Turn on the UV lamp.
Page 69 Benzotriazole/Tolyltriazole (Benzotriazole 1.0 to 16.0 mg/L, Tolyltriazole 1.0 to 20.0 mg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Acrylates (as methyl acrylate) Greater than 50 mg/L Alum Greater than 400 mg/L Borate (as sodium Greater than 4000 mg/L.
Page 70 Benzotriazole/Tolyltriazole (Benzotriazole 1.0 to 16.0 mg/L, Tolyltriazole 1.0 to 20.0 mg/L) 5. Prepare three sample spikes. Fill three mixing cylinders with 25 mL of sample. Use the TenSette Pipet to add 0.1 mL, 0.2 mL, and 0.3 mL of standard, respectively, to each sample and mix thoroughly.
Page 71 Benzotriazole/Tolyltriazole (Benzotriazole 1.0 to 16.0 mg/L, Tolyltriazole 1.0 to 20.0 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Triazole Reagent Powder Pillows 100/pkg 21412-99 Required Apparatus Description Quantity/Test Unit Cat. No. UV Safety Goggles each 21134-00 Sample Cell, 1-in.
Page 72 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 73 Boron Azomethine-H Method Method 10061 Powder Pillows LR (0.02 to 1.50 mg/L as B) Scope and Application: For testing low levels of boron (boric acid or borates) in drinking water, cooling water, industrial process waters, or wastewaters Adapted from ISO Method 9390 Harp, D.L., Analytica Chimica Acta, 346(1997), pp. 373–379. Test Preparation Before starting the test: For best results, match two cells using the...
Page 74 Boron LR (0.02 to 1.50 mg/L as B) 5. Add ten drops of 6. Open one pillow 7. Cap the prepared 8. Press TIMER>OK ™ EDTA Solution, 1 M, to BoroTrace 2 Reagent sample and shake to A ten-minute reaction each cell.
Page 75 Boron LR (0.02 to 1.50 mg/L as B) Cell Matching Procedure 1. Rinse and fill two cells with deionized water. 2. Wipe each cell with a soft cloth or tissue to remove liquid or fingerprints. 3. Using one of the cells, set the instrument absorbance at 410 nm to zero. 4.
Page 76 Boron LR (0.02 to 1.50 mg/L as B) Table 2 lists suggested treatments for interferences: Table 2 Interfering Substances and Suggested Treatments Interfering Substance Interference Levels and Treatments Adjust sample pH to between 5–7 using 1.0 N Sulfuric Acid Solution Alkalinity >500 mg/L (+ or –) Continue with step...
Page 77 Boron LR (0.02 to 1.50 mg/L as B) Table 3 Sample Temperature Multipliers (continued) Sample Temp. Sample Temp. Multiplier Multiplier °C °F °C °F 0.81 1.08 0.84 1.10 0.87 1.12 0.91 1.15 Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify the chemical form.
Page 78 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 79 Boron Carmine Method Method 8015 Powder Pillows (0.2 to 14.0 mg/L) Scope and Application: For water and wastewater Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: All labware must be completely dry. Excess water will cause low results. ®...
Page 80 Boron (0.2 to 14.0 mg/L) Powder Pillows Method 8015 1. Press 2. Select the test. 3. Using a 100-mL 4. Add the contents of graduated cylinder, one BoroVer 3 Reagent STORED PROGRAMS measure 75 mL of Powder Pillow to the flask. concentrated sulfuric acid.
Page 81: Reagent Preparation
Boron (0.2 to 14.0 mg/L) 9. When the timer 10. Wipe the blank and 11. Press 12. Wipe the prepared ZERO expires, pour at least insert the blank into the sample and insert the The display will show: 10 mL from each flask into cell holder with the fill line prepared sample into the separate square sample...
Page 82 Boron (0.2 to 14.0 mg/L) 5. Prepare three sample spikes. Fill three mixing cylinders* with 25 mL of sample. Use the ® TenSette Pipet to add 0.1 mL, 0.2 mL, and 0.3 mL of standard, respectively, to each sample and mix thoroughly. 6.
Page 83 Boron (0.2 to 14.0 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. ® BoroVer 3 Boron Reagent Powder Pillows 100/pkg 14170-99 Sulfuric Acid, ACS, concentrated 75 mL 2.5 L 979-09 Water, deionized 2.0 mL 272-56 Required Apparatus Description Quantity/Test Unit...
Page 84 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 85 Bromine DPD Method Method 8016 ® (0.05 to 4.50 mg/L) Powder Pillows or AccuVac Ampuls Scope and Application: For testing bromine residuals (including hypobromite, hypobromous acid and bromamines) used as disinfectants in process waters, treated water, estuary water, and seawater Adapted from Standard Methods for the Examination of Water and Wastewater Test Preparation Before starting the test:...
Page 86 Bromine (0.05 to 4.50 mg/L) Powder Pillows Method 8016 1. Press 2. Select the test. 3. Fill a square sample 4. Prepared Sample: cell with 10 mL of sample. Add the contents of one STORED PROGRAMS DPD Total Chlorine Powder Pillow to the sample cell.
Page 87 Bromine (0.05 to 4.50 mg/L) ® AccuVac Ampul Method 8016 1. Select the test. 2. Insert Adapter C. 3. Blank Preparation: 4. Prepared Sample: Fill a round 1-inch sample Collect at least 40 mL of cell with 10 mL of sample. sample in a 50-mL beaker.
Page 88 Bromine (0.05 to 4.50 mg/L) Interferences Table 1Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Greater than 150 mg/L CaCO . May not develop full color or color may fade instantly. Neutralize Acidity to pH 6–7 with 1 N Sodium Hydroxide .
Page 89 Bromine (0.05 to 4.50 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. DPD Total Chlorine Reagent Powder Pillows 100/pkg 21056-69 ® DPD Total Chlorine Reagent AccuVac Ampuls 25/pkg 25030-25 Required Apparatus (Powder Pillows) Description Quantity/Test Unit Cat.
Page 90 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 91 Cadmium Dithizone Method Method 8017 Powder Pillows (0 to 80.0 µg/L) Scope and Application: For water and wastewater; digestion is required to determine total cadmium. Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 92 Cadmium (0 to 80.0 µg/L) Powder Pillows Method 8017 DANGER Cyanide is a deadly poison. Use a fume hood. Maintain cyanide solutions at pH 11 or greater to prevent formation of cyanide gas. 1. Press Select the test. 3. Fill a 250-mL 4.
Page 93 Cadmium (0 to 80.0 µg/L) 9. Press 10. Press 11. Prepared Sample: 12. Blank Preparation: TIMER>OK TIMER>OK and allow the funnel to Insert a cotton plug Fill a dry 25-mL sample Close the stopcock and stand undisturbed until the the size of a pea into the cell with chloroform.
Page 94: Sample Collection, Preservation, And Storage
Cadmium (0 to 80.0 µg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Highly buffered samples or May exceed the buffering capacity of the reagents and require sample pretreatment. Extreme sample pH Bismuth Greater than 80 mg/L. See treatment below. Copper Greater than 2 mg/L.
Page 95: Accuracy Check
Cadmium (0 to 80.0 µg/L) Dithiver Solution Preparation and Storage Store DithiVer Powder Pillows away from light and heat. A convenient way to prepare this solution is to add the contents of 16 DithiVer Metals Reagent Powder Pillows to a 500-mL bottle of chloroform and invert several times until well mixed (carrier powder may not dissolve completely).
Page 96 Cadmium (0 to 80.0 µg/L) Pollution Prevention and Waste Management Both chloroform (D022) and cyanide (D003) solutions are regulated as hazardous wastes by the Federal RCRA. Do not pour these solutions down the drain. Chloroform solutions and the cotton plug used in the delivery tube of the separatory funnel should be collected for disposal with laboratory solvent waste.
Page 97 Cadmium (0 to 80.0 µg/L) Recommended Apparatus Description Unit Cat. No. Ampule Breaker Kit each 21968-00 Cylinder, graduated, 5-mL each 508-37 Filter Discs, glass, 47 mm 100/pkg 2530-00 Filter Holder, glass, for 47-mm filter each 2340-00 Flask, Erlenmeyer, 500-mL each 505-49 Flask, filtering, 500-mL each...
Page 98 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 99 Cadmium Cadion Method Method 10217 TNTplus™ 852 (0.02 to 0.30 mg/L Cd) Scope and Application: For wastewater and process control. Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on package.
Page 100 Cadmium (0.02 to 0.30 mg/L Cd) TNTplus Method 10217 1. Pipet 10 mL of sample Pipet 1 mL of 3. Cap the reaction tube 4. Pipet 0.4 mL of into the 20-mm reaction Solution A into the 20-mm and invert 2–3 times. Solution B into a sample tube.
Page 101 Cadmium (0.02 to 0.30 mg/L Cd) Reagent Blanks A reagent blank can be measured, and the value subtracted from the results of each test performed using the same reagent lot number. Use deionized water in place of sample and run the procedure as described.
Page 102 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 103: Chloramine (Mono)
Chloramine (Mono) Indophenol Method HR (0.1 to 10.0 mg/L Cl Method 10172 Scope and Application: Chlorinated wastewater. Patent Pending Test Procedure Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample.
Page 104 Chloramine (Mono) HR (0.1 to 10.0 mg/L Cl 5. Remove the vial from 6. Press 7. After the timer expires, 8. Press TIMER>OK READ the holder. Using a re-insert the vial into the A 5-minute reaction period Results are in mg/L Cl micro-funnel, add the cell holder.
Page 105: Sampling And Storage
Chloramine (Mono) HR (0.1 to 10.0 mg/L Cl Table 1 Non-interfering Substances (continued) Substance Maximum Level Tested Silica 100 mg/L SiO Sulfate 2600 mg/L 2– Sulfite 50 mg/L SO Tyrosine 1 mg/L N Urea 10 mg/L N Zinc 5 mg/L Table 2 describes suggested treatments for interfering substances.
Page 106 Chloramine (Mono) HR (0.1 to 10.0 mg/L Cl 7. Open an ampule and use a glass Mohr pipet to add the calculated amount of Chlorine Solution slowly to the ammonia standard, while mixing at medium speed on a stir-plate. 8. Allow the monochloramine solution to mix for 1 minute after all Chlorine Solution is added. 9.
Page 107 Chloramine (Mono) HR (0.1 to 10.0 mg/L Cl Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. HR Monochloramine Test ‘N Tubes, 50 tests, includes: — — 28051-45 (50) HR Monochloramine Diluent Vials 50/pkg — Funnel, micro each 25843-35 Monochlor F Reagent Pillows 50/pkg...
Page 108 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 109 Chloramine (Mono) Indophenol Method Method 10171 Powder Pillows LR (0.04 to 4.50 mg/L Cl Scope and Application: Chloraminated drinking water and chlorinated wastewater U.S. Patent 6,315,950 Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample.
Page 110 Chloramine (Mono) LR (0.04 to 4.50 mg/L Cl 5. Press 6. Remove the cell and 7. Press 8. After the timer expires, ZERO TIMER>OK add the contents of one insert the vial into the cell The display will show: A 5-minute reaction period Monochlor-F pillow to the holder with the 1-cm (flat) will begin.
Page 111 Chloramine (Mono) LR (0.04 to 4.50 mg/L Cl Table 1 Non-interfering Substances (continued) Substance Maximum Level Tested 2– Sulfite 50 mg/L SO Tyrosine 1 mg/L N Urea 10 mg/L N Zinc 5 mg/L Table 2 Interfering Substances and Suggested Treatments Interfering Substance and Effects Interference Level Recommended Treatment...
Page 112 Chloramine (Mono) LR (0.04 to 4.50 mg/L Cl Accuracy Check To check test accuracy, prepare the following 4.5-mg/L (as Cl ) monochloramine standard immediately before use. 1. Add the contents of one Buffer Powder Pillow, pH 8.3 to about 50-mL of organic-free water in a clean 100-mL Class A volumetric flask.
Page 113 Chloramine (Mono) LR (0.04 to 4.50 mg/L Cl Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Monochlor F Reagent Pillows 50/pkg 28022-46 Required Apparatus Description Quantity/Test Unit Cat. No. Adapter, for Multi-path and Pour-Thru cells each LZV585 Sample Cell, multi-path 6/pkg 59405-06...
Page 114 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 115 Chloramine (Mono); Nitrogen, Free Ammonia Indophenol Method Method 10200 Powder Pillows (0.01–0.50 mg/L NH –N; 0.04–4.50 mg/L Cl Scope and Application: For determining Free Ammonia and Monochloramine simultaneously in finished chloraminated water U.S. Patent 6,315,950 Test Preparation Before starting the test: For more accurate chloramine results, determine a reagent blank value for each new lot of reagent, using deionized water in place of the sample.
Page 116 Chloramine (Mono); Nitrogen, Free Ammonia (0.01–0.50 mg/L NH –N; 0.04–4.50 mg/L Cl 5. Press 6. Remove the cell and 7. Cap and shake the 8. Add one drop of Free ZERO add the contents of one cell about 20 seconds to Ammonia Reagent The display will show: pillow Monochlor-F to the...
Page 117 Chloramine (Mono); Nitrogen, Free Ammonia (0.01–0.50 mg/L NH –N; 0.04–4.50 mg/L Cl 13. Press 14. Select the Free 15. With the 16. Add the contents of READ Ammonia test. monochloramine sample one pillow Monochlor F to Results are in mg/L still in the cell holder, press the cell for the Free Monochloramine (as Cl...
Page 118: Color Development Time
Chloramine (Mono); Nitrogen, Free Ammonia (0.01–0.50 mg/L NH –N; 0.04–4.50 mg/L Cl The following do not interfere in free ammonia determination at or below the stated concentration: Table 1 Non-interfering Substances Substance Maximum Level Tested Aluminum 0.2 mg/L Chloride 1200 mg/L Cl Copper 1 mg/L Cu Iron...
Page 119 Chloramine (Mono); Nitrogen, Free Ammonia (0.01–0.50 mg/L NH –N; 0.04–4.50 mg/L Cl Table 2 Color Development Based on Sample Temperature (continued) Sample Temperature Development Time (minutes) °C °F greater than 25 greater than 77 Sampling and Storage Collect samples in clean glass bottles. Most reliable results are obtained when samples are analyzed as soon as possible after collection.
Page 120 Chloramine (Mono); Nitrogen, Free Ammonia (0.01–0.50 mg/L NH –N; 0.04–4.50 mg/L Cl 9. Quantitatively transfer the monochloramine solution to a clean 100-mL Class A volumetric flask. Dilute to the mark with organic-free water, cap, and mix thoroughly. This is a nominal 4.5-mg/L (as Cl ) monochloramine standard.
Page 121 Chloramine (Mono); Nitrogen, Free Ammonia (0.01–0.50 mg/L NH –N; 0.04–4.50 mg/L Cl Standard Solution Method 1. Prepare a 0.20 mg/L ammonia nitrogen standard by diluting 2.00 mL of the Ammonia Nitrogen Standard Solution, 10 mg/L, to 100 mL with dilution water. Or, using the TenSette Pipet, prepare a 0.20 mg/L ammonia nitrogen standard by diluting 0.4 mL of a Ammonia Nitrogen Voluette Standard Solution, 50 mg/L as NH –N, to 100 mL with dilution water.
Page 122 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 123 Chloride Mercuric Thiocyanate Method Method 8113 – (0.1 to 25.0 mg/L Cl Scope and Application: For water and wastewater Test Preparation Before starting the test: Filter turbid samples with moderately rapid filter paper and a funnel before analysis. Both the sample and the blank will contain mercury (D009) at a concentration regulated as a hazardous waste by the Federal RCRA.
Page 124 – Chloride (0.1 to 25.0 mg/L Cl 5. Pipet 0.8 mL of Swirl to mix. 7. Pipet 0.4 mL of Ferric 8. Swirl to mix. An Mercuric Thiocyanate Ion Solution into each orange color will develop if Solution into each sample sample cell.
Page 125: Accuracy Check
– Chloride (0.1 to 25.0 mg/L Cl Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Press . Press . A summary of the standard OPTIONS>MORE STANDARD ADDITIONS additions procedure will appear.
Page 126 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 127: Chlorine Dioxide
Chlorine Dioxide Direct Reading Method Method 8138 HR (5 to 1000 mg/L) Scope and Application: For water and wastewater Test Preparation Before starting the test: Chlorine dioxide is unstable and volatile. Analyze samples immediately. Collect the following items: Quantity Water, deionized 10 mL Sample cell, 1-inch square glass, 10-mL Note: Reorder information for consumables and replacement items is on page 2.
Page 128 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 129 Chlorine Dioxide Chlorophenol Red Method Method 8065 LR (0.01 to 1.00 mg/L) Scope and Application: For water and wastewater Adapted from Harp, Klein, and Schoonover, Jour. Amer. Water Works Assn., 73 387–388 (1981). Test Preparation Before starting the test: Chlorine dioxide is unstable and volatile. Analyze samples immediately. For most accurate results, analyze each portion of sample at the same temperature.
Page 130 Chlorine Dioxide LR (0.01 to 1.00 mg/L) 5. Blank Preparation: 6. Use a volumetric pipet 7. Invert several times to 8. Use a volumetric pipet Add the contents of one to add exactly 1.00 mL of mix. and pipet filler to add Dechlorinating Reagent Chlorine Dioxide Reagent 1.0 mL of Chlorine Dioxide...
Page 131 Chlorine Dioxide LR (0.01 to 1.00 mg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments May require 2.0 mL each of Chlorine Dioxide Reagent 1 and Chlorine Dioxide Reagent 3 Highly acidic or alkaline water instead of 1.0 mL –...
Page 132 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 133: Stored Programs
Chlorine Dioxide Amaranth Method (20 to 500 µg/L) Scope and Application: For water, drinking water This method is under license of Elf Atofina. Reagent sets for this method are only available in Europe. Test Preparation Before starting the test: Chlorine dioxide is unstable and volatile. Analyze samples immediately. See Sample Collection, Storage, and Preservation on page For most accurate results, analyze each portion of sample at the same temperature.
Page 134 Chlorine Dioxide (20 to 500 µg/L) 5. Pour 10 mL from the 6. Prepared Sample: 7. Fill the second 8. Press TIMER>OK volumetric flask into a Add 1.0 mL of Chlorine volumetric flask to the 1-minute reaction period 10 mL sample cell. Dioxide Reagent A into a mark with the sample.
Page 135 Chlorine Dioxide (20 to 500 µg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments – Greater than 2.0 mg/L – Greater than 2.0 mg/L – Greater than 2.0 mg/L 2– Greater than 0.2 mg/L Greater than 0.5 mg/L Hardness Greater than 1000 mg/L Ozone...
Page 136 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 137 Chlorine Dioxide DPD Method Method 10126 ® Powder Pillows and AccuVac Ampuls (0.04 to 5.00 mg/L) Scope and Application: For water and wastewater. USEPA accepted for reporting for drinking water analysis. Adapted from Standard Methods for the Examination of Water and Wastewater. Procedure is equivalent to Standard Methods, 18 ed., 4500 ClO Test Preparation Before starting the test:...
Page 138 Chlorine Dioxide (0.04 to 5.00 mg/L) Powder Pillows Method 10126 1. Press 2. Select the test. 3. Blank Preparation: 4. Prepared Sample: Fill a square sample cell Fill a second square STORED PROGRAMS with 10 mL of sample and sample cell with 10 mL of stopper.
Page 139: Accuvac ® Ampuls
Chlorine Dioxide (0.04 to 5.00 mg/L) HRS MIN SEC HRS MIN SEC 9. Wait 30 seconds for 10. Within one minute of 11. Press READ any undissolved powder to adding the DPD reagent, Results are in mg/L ClO settle. wipe the sample cell and insert it into the cell holder Immediately proceed to with the fill line facing right.
Page 140 Chlorine Dioxide (0.04 to 5.00 mg/L) 5. Prepared Sample: 6. Fill a DPD Free 7. Quickly invert the 8. Within one minute of Fill a 50-mL beaker with Chlorine Reagent Ampul several times to adding the sample, wipe 40 mL of sample. AccuVac Ampul with mix.
Page 141 Chlorine Dioxide (0.04 to 5.00 mg/L) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Various metals may interfere by combining with the glycine needed to remove the chlorine interference. Metal interference is limited except when chlorine is present. In the presence of 0.6 mg/L Cl , both copper (>10 mg/L) and nickel (>50 mg/L) interfere.
Page 142 Chlorine Dioxide (0.04 to 5.00 mg/L) Accuracy Check Because chlorine dioxide is difficult and hazardous to produce, check the DPD and glycine reagents by using chlorine standards. Proceed as follows: 1. Prepare a 1-mg/L free chlorine standard using Method 1 or 2, below: Method 1 a.
Page 143 Chlorine Dioxide (0.04 to 5.00 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Chlorine Dioxide DPD/Glycine Reagent Set (100 tests), includes: 27709-00 (1) DPD Free Chlorine Reagent Powder Pillows, 10-mL 100/pkg 21055-69 (1) Glycine Reagent 4 drops 29 mL 27621-33 ®...
Page 144 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 145: Chlorine, Free
Chlorine, Free DPD Method Method 8021 ® Powder Pillows or AccuVac Ampuls (0.02 to 2.00 mg/L) Scope and Application: For testing free chlorine (hypochlorous acid and hypochlorite ion) in water, treated waters, estuary, and seawater. USEPA accepted for reporting for drinking water analyses. Adapted from Standard Methods for the Examination of Water and Wastewater.
Page 146 Chlorine, Free (0.02 to 2.00 mg/L) Powder Pillows Method 8021 1. Press 2. Select the test. 3. Blank Preparation: 4. Wipe the blank and Fill a square sample cell insert it into the cell holder STORED PROGRAMS with 10 mL of sample. with the fill line facing right.
Page 147 Chlorine, Free (0.02 to 2.00 mg/L) ® AccuVac Ampuls Method 8021 10 mL 1. Select the test. 2. Insert Adapter C. 3. Blank Preparation: 4. Wipe the blank and Fill a round sample cell insert it into the cell holder. with 10-mL of sample.
Page 148 Chlorine, Free (0.02 to 2.00 mg/L) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Adjust sample pH to 6–7. Add 3 drops Potassium Iodide (30-g/L) to a 10-mL sample. Manganese, Oxidized Mix and wait one minute. , Mn ) or Add 3 drops Sodium Arsenite...
Page 149 Chlorine, Free (0.02 to 2.00 mg/L) 3. A summary of the Standard Additions procedure will appear. Press to accept the values for standard concentration, sample volume, and spike volumes as shown. Press to change these values. After the values are accepted, the unspiked sample reading EDIT will appear in the top row.
Page 150 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 151 Chlorine, Free DPD Method Method 10102 Test ‘N Tube™ Vials (0.09 to 5.00 mg/L) Scope and Application: For testing higher levels of free chlorine (hypochlorous acid and hypochlorite ion) in drinking water, cooling water, and industrial process water. Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 152 Chlorine, Free (0.09 to 5.00 mg/L) 5. Remove the cap from 6. Prepared Sample: 7. Immediately wipe the 8. Press READ a Free Chlorine DPD Test Cap and invert at least 10 sample and insert it in the Results are in mg/L Cl ‘N Tube.
Page 153 Chlorine, Free (0.09 to 5.00 mg/L) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Peroxides May interfere Extreme sample pH or Adjust to pH 6–7 using acid (Sulfuric Acid , 1.000 N) or base (Sodium Hydroxide , 1.00 N).
Page 154 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 155 Chlorine, Free DPD Rapid Liquid Method Method 10059 Pour-Thru™ Cell (0.02 to 2.00 mg/L) Scope and Application: For treated water. Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: Analyze samples immediately. Do not preserve for later analysis. Refer to the instrument User Manual for Pour-Thru cell and module assembly and installation.
Page 156: Reagent Preparation
Chlorine, Free (0.02 to 2.00 mg/L) 5. Add 1.0 mL of Free 6. Add 1.0 mL of 7. Carefully fill the mixing 8. Fill the funnel of the Chlorine Buffer Solution to prepared Free Chlorine cylinder to the 80-mL mark Pour-Thru Cell with the a clean, dry 100-mL glass Indicator Solution to the...
Page 157: Sampling And Storage
Chlorine, Free (0.02 to 2.00 mg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Greater than 400 mg/L CaCO . May not develop full color or color may fade instantly. Alkalinity Neutralize to pH 6–7 with 1 N Sulfuric Acid .
Page 158: Method Performance
Chlorine, Free (0.02 to 2.00 mg/L) Cleaning the Pour-Thru Cell The Pour-Thru Cell may accumulate a buildup of colored reaction products, especially if the reacted solutions are allowed to remain in the cell for long periods after measurement. Remove the buildup by rinsing the cell with 5.25 N Sulfuric Acid* followed by several rinsings with deionized water.
Page 159 Chlorine, Free (0.02 to 2.00 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Free Chlorine Reagent Set, includes: — — 25569-00 DPD Indicator Powder varies 24 g 22972-55 Free Chlorine Indicator Solution 1 mL 473 mL 23140-11 Free Chlorine Buffer Solution 1 mL...
Page 160 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 161 Chlorine, Free DPD Method Method 10069 Powder Pillows HR (0.1 to 10.0 mg/L as Cl Scope and Application: For testing higher levels of free chlorine (hypochlorous acid and hypochlorite ion) in drinking water, cooling water, and industrial process waters Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: Analyze samples immediately.
Page 162 Chlorine, Free HR (0.1 to 10.0 mg/L as Cl 5. Press 6. Remove the cell and 7. Cap and shake the 8. Insert the prepared ZERO add the contents of one cell about 20 seconds to sample into the cell holder The display will show: DPD Free Chlorine dissolve reagent.
Page 163 Chlorine, Free HR (0.1 to 10.0 mg/L as Cl Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments For conventional free chlorine disinfection (beyond the “breakpoint”), typical monochloramine concentrations are very low. If monochloramine is present in the sample, its interference in the free chlorine test is dependent on sample temperature, relative concentration of monochloramine to free chlorine, and the time required to perform the analysis.
Page 164: Standard Additions
Chlorine, Free HR (0.1 to 10.0 mg/L as Cl Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Press . Press . A keypad will appear. Enter the OPTIONS>MORE STANDARD ADDITIONS average chlorine concentration shown on the certificate enclosed with the chlorine...
Page 165 Chlorine, Free HR (0.1 to 10.0 mg/L as Cl Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. DPD Free Chlorine Reagent Powder Pillows for 25-mL samples 100/pkg 14070-99 Required Apparatus Description Quantity/Test Unit Cat. No. Adapter, for Multi-path and Pour-Thru cells each LZV585 Sample cell, multi-path...
Page 166 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 167: Chlorine, Total
Chlorine, Total DPD Method Method 8167 ® Powder Pillows or AccuVac Ampuls (0.02 to 2.00 mg/L) Scope and Application: For testing residual chlorine and chloramines in water, wastewater, estuary water, and seawater; USEPA-accepted for reporting for drinking and wastewater analyses. Adapted from Standard Methods for the Examination of Water and Wastewater Procedure is equivalent to USEPA method 330.5 and Standard Method 4500-Cl G for drinking water and wastewater analyses.
Page 168: Stored Programs
Chlorine, Total (0.02 to 2.00 mg/L) Powder Pillows Method 8167 1. Press 2. Select the test. 3. Fill a square sample 4. Prepared Sample: cell with 10 mL of sample. Add the contents of one STORED PROGRAMS DPD Total Chlorine Powder Pillow to the sample cell.
Page 169 Chlorine, Total (0.02 to 2.00 mg/L) ® AccuVac Ampul Method 8167 10 mL 1. Select the test. 2. Insert Adapter C. 3. Blank Preparation: 4. Prepared Sample: Fill a round sample cell Fill a DPD Total Chlorine ® with 10-mL of sample. Reagent AccuVac Ampul with sample.
Page 170 Chlorine, Total (0.02 to 2.00 mg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Greater than 150 mg/L CaCO . May not develop full color or color may fade instantly. Neutralize to Acidity pH 6 –7 with 1 N sodium hydroxide .
Page 171 Chlorine, Total (0.02 to 2.00 mg/L) Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Press . Press . A keypad will appear. Enter the OPTIONS>MORE STANDARD ADDITIONS average chlorine concentration shown on the certificate enclosed with the Chlorine ®...
Page 172 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 173 Chlorine, Total DPD Method Method 10070 Powder Pillows HR (0.1 to 10.0 mg/L as Cl Scope and Application: For testing higher levels of total chlorine (free and combined) in drinking water, cooling water, and industrial process waters USEPA accepted for reporting drinking water analyses. Procedure is equivalent to USEPA Method 330.5 for Wastewater, and Standard Method 4500-Cl-G for Drinking Water Test Preparation Before starting the test:...
Page 174 Chlorine, Total HR (0.1 to 10.0 mg/L as Cl 5. Press 6. Remove the cell and 7. Press 8. Insert the prepared ZERO TIMER>OK add the contents of one sample into the cell holder The display will show: A 3-minute reaction period DPD Total Chlorine with the 1-cm (flat) path in 0.0 mg/L Cl...
Page 175 Chlorine, Total HR (0.1 to 10.0 mg/L as Cl Sampling and Storage Analyze samples for chlorine immediately after collection. Free and combined chlorine are strong oxidizing agents and react rapidly with various compounds. Many factors such as sunlight, pH, temperature and sample composition will influence decomposition of chlorine in water.
Page 176 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 177 Chlorine, Total DPD Method Method 10101 Test ‘N Tube™ Vials (0.09 to 5.00 mg/L) Scope and Application: For testing higher levels of total (free plus combined) chlorine in drinking water, treated wastewater, cooling water, or industrial process water Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 178 Chlorine, Total (0.09 to 5.00 mg/L) 5. Press 6. Prepared Sample: 7. Cap and slowly invert 8. Press ZERO TIMER>OK Remove the cap from a at least 10 times to The display will show: A two-minute reaction Total Chlorine DPD Test ‘N dissolve the powder.
Page 179 Chlorine, Total (0.09 to 5.00 mg/L) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatment Adjust sample pH to 6–7. Add 3 drops Potassium Iodide (30-g/L) to a 25-mL sample. Manganese, oxidized Mix and wait 1 minute. , Mn Add 3 drops Sodium Arsenite (5-g/L) and mix.
Page 180 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 181 Chlorine, Total DPD Rapid Liquid Method Method 10060 Pour-Thru Cell (0.02 to 2.00 mg/L) Scope and Application: For treated water. Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: Analyze samples immediately. Do not preserve for later analysis. Refer to the instrument User Manual for Pour-Thru cell and module assembly and installation.
Page 182 Chlorine, Total (0.02 to 2.00 mg/L) 5. Add 1.0 mL of Total 6. Add 1.0 mL of 7. Carefully fill the mixing 8. Press TIMER>OK Chlorine Buffer Solution to prepared Total Chlorine cylinder to the 80-mL mark A two-minute reaction a clean, dry 100-mL glass Indicator Solution to the with sample.
Page 183: Reagent Preparation
Chlorine, Total (0.02 to 2.00 mg/L) Reagent Preparation The Total Chlorine Indicator Solution must be prepared before use. Using a powder funnel, add the contents of one 24 g bottle of DPD Powder* to one 473-mL bottle of Total Chlorine Indicator Solution*.
Page 184 Chlorine, Total (0.02 to 2.00 mg/L) Treating Analysis Labware Glassware used in this test must be chlorine demand-free. Fill the 100-mL mixing cylinder and sample container with a dilute solution of chlorine bleach prepared by adding 1 mL of commercial bleach to 1 liter of water. Soak in this solution at least one hour. After soaking, rinse thoroughly with deionized water and allow to dry before use.
Page 185 Chlorine, Total (0.02 to 2.00 mg/L) Summary of Method Chlorine can be present in water as free available chlorine and as combined available chlorine. Both forms can exist in the same water and can be determined together as the total available chlorine.
Page 186 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 187: Dpd Method
Chlorine, Total DPD Method Method 8370 Pour-Thru™ Cell ULR (2 to 500 µg/L as Cl Scope and Application: For detecting trace levels of chlorine and chloramines in clean waters relatively free of color and turbidity; USEPA accepted for reporting for drinking water analysis U.S.
Page 188 Chlorine, Total ULR (2 to 500 µg/L as Cl2) Pour-Thru Cell Method 8370 1. Select the test. 2. Insert Adapter B. 3. Pour at least 50 mL of 4. When the flow stops, Install the Pour-Thru Cell sample into the Pour-Thru press TIMER>OK with the 1-inch (round)
Page 189 Chlorine, Total ULR (2 to 500 µg/L as Cl2) 9. Using a TenSette 10. Prepared Sample: 11. Press 12. Introduce the contents TIMER>OK Pipet and a clean tip, Avoiding extra agitation, of the graduated mixing A three-minute reaction transfer 1.0 mL of indicator carefully fill the cylinder to cylinder into the Pour-Thru time will begin.
Page 190 Chlorine, Total ULR (2 to 500 µg/L as Cl2) Determining the Reagent Blank Value Reagent Blank: 1. Select the test. 2. Make sure that the 3. Insert Adapter B. 4. Collect about 100 mL reagent blank setting is off. Install the Pour-Thru of deionized or tap water module and cell with the in a clean, 250-mL beaker.
Page 191 Chlorine, Total ULR (2 to 500 µg/L as Cl2) 9. Fill the cylinder to the 10. Press 11. During the reaction 12. When the flow stops, TIMER>OK 50-mL mark with period, flush the Pour-Thru press ZERO A three-minute reaction dechlorinated water from Cell with the remainder of time will begin.
Page 192 Chlorine, Total ULR (2 to 500 µg/L as Cl2) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Adjust sample pH to 6 –7 with 1.000 N Sulfuric Acid Add 9 drops Potassium Iodide (30 g/L) to an 80-mL sample.
Page 193 Chlorine, Total ULR (2 to 500 µg/L as Cl2) Cleaning the Pour-Thru Cell The Pour-Thru Cell may accumulate a buildup of colored reaction products, especially if the reacted solutions are allowed to remain in the cell for long periods after measurement. Remove the buildup by rinsing the cell with 5.25 N Sulfuric Acid* followed by several rinsings with deionized water.
Page 194 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 195 Chlorine, Total DPD Method Method 10014 Pour-Thru Cell and OriFlo™ Filtration ULR (2 to 500 µg/L as Cl Scope and Application: For detecting trace levels of chlorine and chloramines in clean waters relatively free of color and turbidity; USEPA accepted for reporting for wastewater analysis Adapted from Standard Methods for the Examination of Water and Wastewater.
Page 196 Chlorine, Total ULR (2 to 500 µg/L as Cl Pour-Thru Cell Method 8370 1. Select the test. 2. Insert Adapter B. 3. Unscrew the cap from 4. Install a new, 3-micron Install the Pour-Thru Cell the OriFlo™ plunger filter into the cap well. Wet with the 1-inch (round) assembly.
Page 197 Chlorine, Total ULR (2 to 500 µg/L as Cl 9. Prepared Sample: 10. Press 11. Push the valve button 12. Insert the plunger into TIMER>OK ™ Avoiding extra agitation, on the OriFlo barrel the barrel and slowly push A three-minute reaction carefully fill the cylinder to assembly to the “closed”...
Page 198 Chlorine, Total ULR (2 to 500 µg/L as Cl 17. When the timer 18. Insert the plunger into 19. Pour the filtered, 20. Flush the Pour-Thru expires, pour the contents the barrel and slowly push reacted sample from the Cell with at least 50-mL of of the mixing cylinder into the plunger down with plunger reservoir into the...
Page 199 Chlorine, Total ULR (2 to 500 µg/L as Cl GENERAL TIMER 5. Using a TenSette 6. Press 7. After the timer expires, 8. Break open one Pipet, add 1.0 mL of break open one ampule of ampule of DPD Indicator OPTIONS>MORE>TIMER>...
Page 200 Chlorine, Total ULR (2 to 500 µg/L as Cl 13. When the timer 14. Use this value to 15. Flush the Pour-Thru expires, introduce the correct the sample result Cell with at least 50-mL of contents of the cylinder obtained in this procedure. deionized water into the Pour-Thru Cell.
Page 201 Chlorine, Total ULR (2 to 500 µg/L as Cl Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Ozone Interferes at all levels. Peroxides May interfere Extreme sample pH or highly Adjust to pH 6 –7 buffered samples Optional Reagents and Apparatus on page Samples treated with sodium arsenite for interferences will be hazardous waste as regulated by the Federal RCRA for arsenic (D004).
Page 202: Standard Additions
Chlorine, Total ULR (2 to 500 µg/L as Cl Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Press . Press . A keypad will appear. Enter the OPTIONS>MORE STANDARD ADDITIONS average chlorine concentration shown on the certificate enclosed with the chlorine...
Page 203 Chlorine, Total ULR (2 to 500 µg/L as Cl Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. ULR Chlorine Reagent Set (approximately 20 tests), includes: — — 25630-00 ULR Chlorine Buffer Solution, 1.5-mL ampules 1 mL 20/pkg 24931-20 DPD Indicator Solution for ULR Chlorine, 1.5-mL ampules 1 mL...
Page 204 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 205: Chromium, Hexavalent
Chromium, Hexavalent 1,5-Diphenylcarbohydrazide Method Method 8023 ® (0.010 to 0.700 mg/L Cr Powder Pillows or AccuVac Ampuls Scope and Application: For water and wastewater; USEPA accepted for reporting for wastewater analysis Adapted from Standard Methods for the Examination of Water and Wastewater. Procedure is equivalent to USGS method 1-1230-85 for wastewater.
Page 206: Powder Pillows
Chromium, Hexavalent (0.010 to 0.700 mg/L Cr Powder Pillows Method 8023 1. Press Select the test. 3. Fill a square sample 4. Prepared Sample: cell with 10 mL of sample. Add the contents of one STORED PROGRAMS ® ChromaVer 3 Reagent Powder Pillow to the sample cell.
Page 207 Chromium, Hexavalent (0.010 to 0.700 mg/L Cr ® AccuVac Ampul Method 8023 10 mL 1. Select the test. 2. Insert Adapter C. 3. Blank Preparation: 4. Prepared Sample: Fill a round sample cell Fill a ChromaVer 3 ® with 10-mL of sample. Reagent AccuVac Ampul with sample from the...
Page 208: Sample Collection, Preservation, And Storage
Chromium, Hexavalent (0.010 to 0.700 mg/L Cr Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Iron May interfere above 1 mg/L Mercurous & Mercuric Ions Interfere slightly Highly buffered samples or extreme sample pH may exceed the buffering capacity of the reagents and require sample pretreatment.
Page 209 Chromium, Hexavalent (0.010 to 0.700 mg/L Cr Standard Solution Method Prepare a 0.50-mg/L Cr standard solution daily, as follows: 1. Using a 5.00 mL pipet transfer 5.00 mL of Hexavalent Chromium Standard Solution, 50 mg/L, into a Class A 500-mL volumetric flask. 2.
Page 210 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 211: Chromium, Total
Chromium, Total Alkaline Hypobromite Oxidation Method Method 8024 Powder Pillows (0.01 to 0.70 mg/L) Scope and Application: For water and wastewater Adapted from Standard Methods for the Examination of Water and Wastewater This procedure is equivalent to Standard Method 3500-CRD for wastewater. Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 212 Chromium, Total (0.01 to 0.70 mg/L) 5. Insert the prepared 6. Press 7. When the timer 8. Remove cap and add TIMER>OK sample into a boiling water expires, remove the the contents of one A five-minute reaction bath. prepared sample. Using Chromium 2 Reagent period will begin.
Page 213 Chromium, Total (0.01 to 0.70 mg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Highly buffered samples or May exceed the buffering capacity of the reagents and require sample pretreatment. extreme sample pH May inhibit complete oxidation of trivalent chromium. If high levels of organic material are Organic material present, digestion may be required.
Page 214 Chromium, Total (0.01 to 0.70 mg/L) Standard Solution Method Prepare a 0.50-mg/L trivalent chromium standard as follows: 1. Dilute 5.00 mL of Trivalent Chromium Standard Solution, 50-mg/L as Cr , to 500 mL with deionized water. Prepare this solution daily. 2.
Page 215 Chromium, Total (0.01 to 0.70 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Total Chromium Reagent Set (100 tests), includes: — — 22425-00 Acid Reagent Powder Pillows 100/pkg 2126-99 ® ChromaVer 3 Chromium Reagent Powder Pillows 100/pkg 12066-99 Chromium 1 Reagent Powder Pillows...
Page 216 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 217 Chromium, Total and Hexavalent Method 10218 (Chromium, Hexavalent) 1,5-Diphenylcarbohydrazide Method Method 10219 (Chromium, Total) TNTplus™ 854 (0.03 to 1.00 mg/L Cr) Scope and Application: For wastewater and process analysis Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Recommended sample pH is 3–9.
Page 218 Chromium, Total and Hexavalent (0.03 to 1.00 mg/L Cr) TNTplus Chromium, Total Method 10219 1. Turn on the DRB200 2. Carefully remove the 3. Pipet 2.0 mL of 4. Flip the DosiCap Zip Reactor. Preheat to protective foil lid from the sample into the vial.
Page 219 Chromium, Total and Hexavalent (0.03 to 1.00 mg/L Cr) HRS MIN SEC HRS MIN SEC 9. Invert the vial 2-3 10. After inverting the 11. After the timer expires, 12. Thoroughly clean the times to mix. tube, allow the vial to sit invert the vial again 2–3 outside of the vial.
Page 220 Chromium, Total and Hexavalent (0.03 to 1.00 mg/L Cr) 5. After the timer expires, 6. Thoroughly clean the 7. Insert the prepared invert the vial again 2–3 outside of the vial. vial into the cell holder. times. The instrument reads the barcode, then selects and performs the correct test.
Page 221 Chromium, Total and Hexavalent (0.03 to 1.00 mg/L Cr) Interferences The ions listed in Table 1 have been individually checked up to the given concentrations and do not cause interference. Cumulative effects and the influence of other ions have not been determined.
Page 222 Chromium, Total and Hexavalent (0.03 to 1.00 mg/L Cr) Standard Solution Method (Hexavalent Chromium) To check the accuracy of the hexavalent chromium method, prepare a 0.50-mg/L hexavalent chromium standard as follows: 1. Dilute 5.00 mL of Hexavalent Chromium Standard Solution, 10 mg/L as Cr , to 100 mL with deionized water.
Page 223 Chromium, Total and Hexavalent (0.03 to 1.00 mg/L Cr) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Chromium, Total and Hexavalent TNTplus, TNT854 Reagent Set 25/pkg TNT854 Required Apparatus Description Quantity/Test Unit Cat. No. DRB200 Reactor, 115 V, 13x17 mm + 2x20 mm (mono block) each DRB200-01 DRB200 Reactor, 230 V, 9x13mm + 2x20 mm (mono block)
Page 224 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 225 Cobalt 1-(2-Pyridylazo)-2-Naphthol (PAN) Method Method 8078 Powder Pillows (0.01 to 2.00 mg/L) Scope and Application: For water and wastewater; digestion is required for determining total recoverable cobalt; if EDTA is present, use the vigorous digestion. Adapted from Watanbe, H., Talanta, 21 295 (1974) Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 226 Cobalt (0.01 to 2.00 mg/L) 5. Add the contents of 6. Use the plastic 7. Stopper the cells. 8. Press TIMER>OK one Phthalate-Phosphate dropper provided to add Invert several times to mix. A three-minute reaction Reagent Powder Pillow to 0.5 mL of 0.3% PAN period will begin.
Page 227 Cobalt (0.01 to 2.00 mg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments 32 mg/L 1000 mg/L as CaCO 20 mg/L – 8000 mg/L 20 mg/L 40 mg/L 15 mg/L – 20 mg/L Interferes directly and must not be present 10 mg/L 500 mg/L 400 mg/L...
Page 228 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 229: Color, True And Apparent
Color, True and Apparent Platinum-Cobalt Standard Method Method 8025 (15 to 500 units) Scope and Application: For water, wastewater, and seawater; equivalent to NCASI method 253 for pulp and paper effluent using 465 nm (requires pH adjustment) Adapted from Standard Methods for the Examination of Water and Wastewater and NCASI, Technical Bulletin No. 253, Dec. 1971 Adapted from Wat.
Page 230 Color, True and Apparent (15 to 500 units) 4. Rinse the filter by 5. Pour another 50 mL of 6. Blank Preparation: 7. Pour about 50 mL of pouring about 50 mL of deionized water through Fill a square sample cell sample through the filter.
Page 231 Color, True and Apparent (15 to 500 units) Sample Collection, Storage, and Preservation Collect samples in clean plastic or glass bottles. Most reliable results are obtained when samples are analyzed as soon as possible after collection. If prompt analysis is impossible, fill bottles completely and cap tightly.
Page 232 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 233 Copper Porphyrin Method Method 8143 Powder Pillows LR (1 to 210 µg/L) Scope and Application: For water, wastewater, and sea water Adapted from Ishii and Koh, Bunseki Kagaku, 28 (473), 1979 Test Preparation Before starting the test: Digestion is required for determining total copper. For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 234 Copper LR (1 to 210 µg/L) 5. Add the contents of 6. Swirl to dissolve. 7. Add the contents of 8. Swirl to dissolve. one Porphyrin 1 Reagent one Porphyrin 2 Reagent If copper is present, the Powder Pillow to each Powder Pillow to each sample will momentarily sample cell.
Page 235 Copper LR (1 to 210 µg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Aluminum, Al 60 mg/L Cadmium, Cd 10 mg/L Calcium, Ca 1500 mg/L Chelating agents Interfere at all levels unless either the Digesdahl or vigorous digestion is performed Chloride, Cl –...
Page 236 Copper LR (1 to 210 µg/L) 4. Press to accept the default values for standard concentration, sample volume, and spike volumes. Press to change these values. After values are accepted, the EDIT unspiked sample reading will appear in the top row. See the user manual for more information.
Page 237 Copper LR (1 to 210 µg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Copper Reagent Set (100 tests), includes: — — 26033-00 (1) Copper Masking Reagent Powder Pillows 100/pkg 26034-49 (2) Porphyin 1 Reagent Powder Pillows 100/pkg 26035-49 (2) Porphyrin 2 Reagent Powder Pillows...
Page 238 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 239 Copper Bicinchoninate Method Method 8506 and Method 8026 Powder Pillows or AccuVac Ampuls (0.04 to 5.00 mg/L) ® Scope and Application: For water, wastewater and seawater ; Method 8506 USEPA approved for reporting wastewater analysis (digestion required) Adapted from Nakano, S., Yakugaku Zasshi, 82 486-491 (1962) [Chemical Abstracts, 58 3390e (1963)] Pretreatment required;...
Page 240: Stored Programs
Copper (0.04 to 5.00 mg/L) Powder Pillows Method 8506 1. Press 2. Select the test. 3. Prepared Sample: Fill 4. Add the contents of ® a square sample cell with one CuVer 1 Copper STORED PROGRAMS 10 mL of sample. Reagent Powder Pillow to the sample cell (the prepared sample).
Page 241 Copper (0.04 to 5.00 mg/L) ® AccuVac Ampul Method 8026 10 mL 1. Select the test. 2. Insert Adapter C. 3. Blank Preparation: 4. Prepared Sample: Fill a round sample cell Collect at least 40 mL of with 10-mL of sample. sample in a 50-mL beaker.
Page 242 Copper (0.04 to 5.00 mg/L) Table 1 Interfering Substances and Suggested Treatments for Powder Pillows Interfering Substance Interference Levels and Treatments If the sample is extremely acidic (pH 2 or less) a precipitate may form. Add 8 N Potassium Acidity Hydroxide Standard Solution drop-wise until sample pH is above 4.
Page 243 Copper (0.04 to 5.00 mg/L) Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Press . Press . A summary of the standard OPTIONS>MORE STANDARD ADDITIONS additions procedure will appear. 3.
Page 244 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 245 Cyanide Pyridine-Pyrazalone Method Method 8027 – Powder Pillows (0.002 to 0.240 mg/L CN Scope and Application: For water, wastewater, and seawater Adapted from Epstein, Joseph, Anal. Chem. 19(4), 272 (1947) Test Preparation Before starting the test: Use a water bath to maintain the optimum temperature for the reaction in this test (25 °C). Samples at less than 23 °C require longer reaction times, and samples at greater than 25 °C yield low results.
Page 246 – Cyanide (0.002 to 0.240 mg/L CN 5. Shake the sample cell 6. Leave the sample cell 7. Add the contents of 8. Shake the sample for for 30 seconds. undisturbed for an one CyaniVer 4 Cyanide 10 seconds. Immediately additional 30 seconds.
Page 247: Pollution Prevention And Waste Management
– Cyanide (0.002 to 0.240 mg/L CN Pollution Prevention and Waste Management Special Considerations for Cyanide Containing Materials Samples analyzed by this procedure may contain cyanide, which is regulated as reactive (D003) waste by the federal RCRA. It is imperative these materials be handled safely to prevent the release of hydrogen cyanide gas (an extremely toxic material with the smell of almonds).
Page 248 – Cyanide (0.002 to 0.240 mg/L CN Table 1 Interfering Substances and Levels (continued) Interfering Interference Levels and Treatments Substance Adjust a 25-mL portion of the alkaline sample to pH 7–9 with 2.5 N Hydrochloric Acid Standard Solution Count the number of drops added. Add four drops of Potassium Iodide Solution (Cat.
Page 249: Acid Distillation
– Cyanide (0.002 to 0.240 mg/L CN 4. If step 3 suggests the presence of oxidizing agents, add two level, 1-g measuring spoonfuls of Ascorbic Acid* per liter of sample. 5. Withdraw a 25-mL portion of sample treated with ascorbic acid and repeat steps 1 to 3. If the sample turns blue, repeat steps 4 and 5.
Page 250 – Cyanide (0.002 to 0.240 mg/L CN If cyanide is not present, the amount of thiocyanate can be determined. The sample is not – distilled and the final reading is multiplied by 2.2. The result is mg/L SCN The distillate can be tested and treated for sulfide after the last step of the distillation procedure by using the following lead acetate treatment procedure.
Page 251 – Cyanide (0.002 to 0.240 mg/L CN 13. After 15 minutes, remove the rubber stopper on the 500-mL vacuum flask to break the vacuum and turn off the water to the aspirator. Turn off the water to the condenser. 14. Remove the gas bubbler/cylinder assembly from the distillation apparatus. Separate the gas bubbler from the cylinder and pour the contents of the cylinder into a 250-mL, Class A volumetric flask.
Page 252 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 253: Cyanuric Acid
Cyanuric Acid Turbidimetric Method Method 8139 Powder Pillows (5 to 50 mg/L) Scope and Application: For water Test Preparation Before starting the test: Filter highly turbid samples with filter paper and a funnel. Clean sample cells with soap, water, and a brush soon after each test to avoid a build-up of film on the sample cell. Collect the following items: Quantity Bottle, mixing, square, glass...
Page 254 Cyanuric Acid (5 to 50 mg/L) 5. Press 6. Blank Preparation: 7. When the timer 8. Within seven minutes TIMER>OK Fill a square sample cell expires, fill a second after the timer expires, A 3-minute reaction period with 10 mL of sample and square sample cell with place the prepared sample will begin.
Page 255 Cyanuric Acid (5 to 50 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Cyanuric Acid 2 Reagent Powder Pillow 50/pkg 2460-66 Required Apparatus Description Quantity/Test Unit Cat. No. Bottle, mixing, square, with 25 mL mark each 17042-00 Sample Cell, 1-inch square, 10-mL matched pair 2/pkg...
Page 256 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 257 Fluoride SPADNS Method Method 8029 ® – Reagent Solution or AccuVac Ampuls (0.02 to 2.00 mg/L F Scope and Application: For water, wastewater and seawater; USEPA accepted for reporting for drinking and wastewater analyses (distillation required; see Distillation on page 4) Adapted from Standard Methods for the Examination of Water and Wastewater, 4500-F B &...
Page 258: Stored Programs
– Fluoride (0.02 to 2.00 mg/L F Using SPADNS Reagent Method 8029 1. Press 2. Select the test. 3. Prepared Sample: 4. Blank Preparation: Pipet 10.0 mL of sample Pipet 10.0 mL of deionized STORED PROGRAMS into a dry square sample water into a second dry cell.
Page 259 – Fluoride (0.02 to 2.00 mg/L F ® AccuVac Ampul Method 8029 1. Select the test. 2. Insert Adapter C. 3. Prepared Sample: 4. Blank Preparation: Collect at least 40 mL of Pour at least 40 mL of sample in a 50-mL beaker. deionized water into a second beaker.
Page 260 – Fluoride (0.02 to 2.00 mg/L F Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments At 5000 mg/L it causes a −0.1 mg/L F – Alkalinity (as CaCO error. At 0.1 mg/L it causes a −0.1 mg/L F –...
Page 261 – Fluoride (0.02 to 2.00 mg/L F Sample Collection, Storage, and Preservation Samples may be stored in glass or plastic bottles for at least seven days when cooled to 4 °C (39 °F) or lower. Warm samples to room temperature before analysis. Accuracy Check Standard Solution Method A variety of standard solutions covering the entire range of the test is available.
Page 262 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 263 Formaldehyde MBTH Method Method 8110 Powder Pillows (3 to 500 µg/L) Scope and Application: For water Adapted from Matthews, T.G. and Howell, T.C., Journal of the Air Pollution Control Association, 31 (11) 1181-1184 (1981). Test Preparation Before starting the test: Analyze samples immediately.
Page 264 Formaldehyde (3 to 500 µg/L) Powder Pillows Method 8110 1. Press 2. Select the test. 3. Prepared Sample: 4. Blank Preparation: Accurately measure 25 mL Accurately measure 25 mL STORED PROGRAMS of sample in a 50-mL of formaldehyde-free mixing cylinder. water in a second 50-mL mixing cylinder.
Page 265 Formaldehyde (3 to 500 µg/L) 9. Stopper the cylinder 10. Add 2.5 mL of 11. Add 2.5 mL of 12. Just before the timer and shake vigorously for Developing Solution for Developing Solution shows 2:00, pour at least 20 seconds. Low Range Formaldehyde for Low Range 10 mL of the blank into the...
Page 266: Standard Additions
Formaldehyde (3 to 500 µg/L) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Cyclohexylamine Greater than 250 mg/L Ethanolamine Greater than 33 mg/L Ethylenediamine Greater than 1.5 mg/L Glucose Greater than 1000 mg/L Glycine Greater than 1000 mg/L Iron (Fe Greater than 12 mg/L Lead...
Page 267 Formaldehyde (3 to 500 µg/L) 8. After completing the sequence, press to view the best-fit line through the standard GRAPH additions data points, accounting for the matrix interferences. Press to view IDEAL LINE the relationship between the sample spikes and the “Ideal Line” of 100% recovery Standard Solution Method Prepare a 320-µg/L Formaldehyde Standard Solution by pipetting 1.0 mL of the 8000-µg/L solution into a 50-mL mixing cylinder.
Page 268 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 269 Hardness Calcium and Magnesium; Calmagite Colorimetric Method Method 8030 (0.05 to 4.00 mg/L Ca and Mg as CaCO Scope and Application: For water, wastewater, and seawater Test Preparation Before starting the test: For the most accurate magnesium test results, keep the sample temperature between 21–29 °C (70–84 °F). The test will detect any calcium or magnesium contamination in the mixing cylinder, measuring droppers, or sample cells.
Page 270 Hardness (0.05 to 4.00 mg/L Ca and Mg as CaCO 5. Stopper the cylinder 6. Add 1.0 mL of Alkali 7. Stopper the cylinder 8. Pour 10 mL of the and invert it several times. Solution for Calcium and and invert it several times. solution into each of three Magnesium Test using a square sample cells.
Page 271 Hardness (0.05 to 4.00 mg/L Ca and Mg as CaCO 13. Do not remove the cell 14. Press 15. Press 16. Calcium Sample: EXIT ZERO from the instrument. Insert the third cell into Select the test. The display will show: Record or store the the cell holder with the fill magnesium results before...
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Page 273 Hardness Calcium and Magnesium; Chlorophosphonazo Colorimetric Method Method 8374 (8 to 1000 µg/L Ca and Mg as CaCO Scope and Application: For water, wastewater, and seawater Test Preparation Before starting the test: For the most accurate magnesium test results, keep the sample temperature between 21–29 °C (70–84 °F). The test will detect any calcium or magnesium contamination in the mixing cylinder, measuring droppers, or sample cells.
Page 274 Hardness (8 to 1000 µg/L Ca and Mg as CaCO 5. Add the contents of 6. Cap the cell and swirl 7. Insert the cell into the 8. Press ZERO one Chlorophosphonazo to mix. cell holder. The display will show: Solution Pillow to the 0 µg/L CaCO sample cell.
Page 275 Hardness (8 to 1000 µg/L Ca and Mg as CaCO Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Silicon Positive interference above 1000 µg/L Sodium Negative interference above 79,000 µg/L Sample Collection, Storage, and Preservation Do not use glass containers.
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Page 277: Hardness, Total
Hardness, Total Calcium and Magnesium; Chlorophosphonazo Rapid Liquid Method Method 8374 Pour-Thru Cell ULR (4 to 1000 µg/L Ca & Mg as CaCO Scope and Application: For boiler, cooling, and ultra pure water Test Preparation Before starting the test: Pre-clean the Pour-Thru Cell and all labware as specified in Treating Analysis Labware on page Protect the Pour-Thru Cell from contamination when not in use by inverting a small beaker over the top of the glass funnel.
Page 278 Hardness, Total ULR (4 to 1000 µg/L Ca & Mg as CaCO Pour-Thru Cell Method 8374 1. Select the test. 2. Insert Adapter B. 3. Fill a clean, 125-mL 4. Rinse a clean, 50-mL Install the Pour-Thru Cell plastic Erlenmeyer flask to plastic graduated cylinder with the 1-inch round path overflowing with sample.
Page 279 Hardness, Total ULR (4 to 1000 µg/L Ca & Mg as CaCO 9. After the flow stops, 10. Add one drop of CDTA 11. Pour the remaining 12. After the flow stops, press Reagent for Ultra Low sample into the Pour-Thru press .
Page 280: Sampling And Storage
Hardness, Total ULR (4 to 1000 µg/L Ca & Mg as CaCO Interferences Interference studies were conducted at various hardness levels between 0 and 500 µg/L as CaCO . Various cations and anions were evaluated at levels in the range appropriate to ultra pure water applications.
Page 281 Hardness, Total ULR (4 to 1000 µg/L Ca & Mg as CaCO Accuracy Check Standard Additions Method (Sample Spike) 1. Leave the unspiked sample in the sample cell compartment. Press OPTIONS>MORE Press . A summary of the standard additions procedure STANDARD ADDITIONS will appear.
Page 282 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 283 Hydrazine p-Dimethylaminobenzaldehyde Method Method 8141 ® Reagent Solution or AccuVac Ampuls (4 to 600 µg/L) Scope and Application: For boiler water/feedwater Adapted from ASTM Manual of Industrial Water, D1385-78, 376 (1979) Test Preparation Before starting the test: Samples cannot be preserved and must be analyzed immediately Sample temperature should be 21 ±...
Page 284 Hydrazine (4 to 600 µg/L) 5. Add 0.5 mL of 6. Press 7. Insert the blank into 8. Insert the prepared TIMER>OK HydraVer 2 Hydrazine the cell holder with the fill sample into the cell holder. A 12-minute reaction Reagent to each sample line facing right.
Page 285 Hydrazine (4 to 600 µg/L) 5. Blank Preparation: 6. Insert the blank into 7. Press 8. Insert the prepared ZERO Pour at least 40-mL of the cell holder. sample in the cell holder. The display will show: deionized water into a Immediately after the second beaker.
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Page 287: Powder Pillows
Iodine DPD Method Method 8031 ® Powder Pillows or AccuVac Ampuls (0.07 to 7.00 mg/L) Scope and Application: For testing dissolved iodine residual used as disinfectant in process water, treated water, estuary water, and seawater Adapted from Palin, A.T., Inst. Water Eng., 21 (6), 537-547 (1967). Test Preparation Before starting the test: Analyze samples immediately.
Page 288 Iodine (0.07 to 7.00 mg/L) 5. Press 6. Blank Preparation: 7. Wipe the blank and 8. Within three minutes TIMER>OK Fill a second square insert it into the cell holder after the timer expires, A three-minute reaction sample cell with 10 mL of with the fill line facing right.
Page 289 Iodine (0.07 to 7.00 mg/L) 5. Press 6. Blank Preparation: 7. Wipe the blank and 8. Within three minutes TIMER>OK Fill a round sample cell insert it into the cell holder. after the timer expires, wipe A three-minute reaction with 10 mL of sample. Press .
Page 290 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 291 Iron ® FerroZine Method Method 8147 ® FerroZine Reagent Solution Pillows (0.009 to 1.400 mg/L) Scope and Application: For water and wastewater Adapted from Stookey, L.L., Anal. Chem., 42(7), 779 (1970) Test Preparation Before starting the test: Digestion is required for total iron determination. Rinse glassware with a 1:1 hydrochloric acid solution.
Page 292: Stored Programs
Iron (0.009 to 1.400 mg/L) Solution Pillows Method 8147 1. Press 2. Select the test. 3. Fill a clean 25-mL 4. Prepared Sample: graduated mixing cylinder Add the contents of one STORED PROGRAMS ® to the 25-mL mark with FerroZine Iron Reagent sample.
Page 293 Iron (0.009 to 1.400 mg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments ® Interfere at all levels. Use the FerroVer or TPTZ methods for these samples. Use the TPTZ Strong chelants (EDTA) method for low iron concentrations. Cobalt May give slightly high results Copper...
Page 294 Iron (0.009 to 1.400 mg/L) Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Press . Press . A summary of the standard OPTIONS>MORE STANDARD ADDITIONS additions procedure will appear. 3.
Page 295 Iron (0.009 to 1.400 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. ® FerroZine Iron Reagent Solution, or 0.5 mL 500 mL 2301-49 ® FerroZine Iron Reagent Solution Pillows 50/pkg 2301-66 Required Apparatus Description Quantity/Test Unit Cat.
Page 296 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 297: Iron, Ferrous
Iron, Ferrous 1, 10 Phenanthroline Method Method 8146 ® Powder Pillows or AccuVac Ampuls (0.02 to 3.00 mg/L) Scope and Application: For water, wastewater, and seawater Adapted from Standard Methods for the Examination of Water and Wastewater, 15th ed. 201 (1980) Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 298 Iron, Ferrous (0.02 to 3.00 mg/L) 5. Press 6. Blank Preparation: 7. Fill a second square 8. When the timer TIMER>OK Fill a square cell with 10 sample cell with the expires, insert the blank A three-minute reaction mL of sample. prepared sample from the into the cell holder with the period will begin.
Page 299 Iron, Ferrous (0.02 to 3.00 mg/L) AccuVac Ampul Method 8146 10 mL 1. Select the test. 2. Insert Adapter C. 3. Blank Preparation: 4. Prepared Sample: Fill a round sample cell Collect at least 40 mL of with 10 mL of sample. sample in a 50-mL beaker.
Page 300 Iron, Ferrous (0.02 to 3.00 mg/L) Sample Collection, Storage, and Preservation Collect samples in plastic or glass bottles. Analyze samples as soon as possible after collection. Accuracy Check Standard Solution Method 1. Prepare a ferrous iron stock solution (100-mg/L Fe ) by dissolving 0.7022 grams of Ferrous Ammonium Sulfate, hexahydrate, in deionized water.
Page 301 Iron, Ferrous (0.02 to 3.00 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Ferrous Iron Reagent Powder Pillows 100/pkg 1037-69 ® Ferrous Iron Reagent AccuVac Ampuls 25/pkg 25140-25 Required Apparatus (Powder Pillows) Description Quantity/Test Unit Cat. No. Sample Cells, 1-inch square, 10 mL, matched pair 2/pkg 24954-02...
Page 302 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 303 Iron ® FerroZine Rapid Liquid Method* Method 8147 Pour-Thru Cell (0.009 to 1.400 mg/L Fe) Scope and Application: For boiler, cooling, and natural waters Adapted from Stookey, L.L., Anal.Chem., 42 (7) 779 1970. Test Preparation Before starting the test: If sample contains rust, see Interferences on page Digestion is required for total iron determination.
Page 304 Iron (0.009 to 1.400 mg/L Fe) Pour-Thru Cell Method 8147 1. Select the test. 2. Insert Adapter B. 3. Flush the Pour-Thru 4. Rinse two clean Install the Pour-Thru Cell Cell with 50 mL of 125-mL Erlenmeyer flasks with the 1-inch pathlength deionized water.
Page 305 Iron (0.009 to 1.400 mg/L Fe) 13. Pour the contents of 14. When the flow stops, 15. Flush the Pour-Thru the flask containing the press Cell with at least 50 mL READ prepared sample into the of deionized water Results are in mg/L Fe. Pour-Thru Cell.
Page 306 Iron (0.009 to 1.400 mg/L Fe) Sample Collection, Storage, and Preservation Collect samples in acid-washed glass or plastic bottles. To preserve samples, adjust the sample pH to 2 or less with concentrated Nitric Acid, ACS* (about 2 mL per liter). Samples preserved in this manner can be stored up to six months at room temperature.
Page 307 Iron (0.009 to 1.400 mg/L Fe) Standard Solutions Method To check the accuracy, use a 1.0 mg/L Iron Standard Solution or prepare a 1.0 mg/L iron working solution as follows: 1. Pipet 5.00 mL of iron standard solution, 100-mg/L Fe, into a 500-mL volumetric flask. 2.
Page 308 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 309: Iron, Total
Iron, Total TPTZ Method Method 8112 ® Powder Pillows or AccuVac Ampuls (0.012 to 1.800 mg/L) Scope and Application: For water, wastewater, and seawater Adapted from G. Frederic Smith Chemical Co., The Iron Reagents, 3rd ed. (1980) Test Preparation Before starting the test: Digestion is required for determining total iron.
Page 310 Iron, Total (0.012 to 1.800 mg/L) 5. Add the contents of 6. Press 7. When the timer 8. Insert the prepared TIMER>OK one 10-mL TPTZ Iron expires, insert the blank sample into the cell holder A three-minute reaction Reagent Powder Pillow to into the cell holder with the with the fill line facing right.
Page 311 Iron, Total (0.012 to 1.800 mg/L) Interferences Interference tests (Table 1) were performed using an iron concentration of 0.5 mg/L. When interferences occurred, the color formation was inhibited or a precipitate formed. The following do not interfere with the test when present up to the levels given. Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments...
Page 312 Iron, Total (0.012 to 1.800 mg/L) Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Press . Press . A summary of the standard OPTIONS>MORE STANDARD ADDITIONS additions procedure will appear.
Page 313 Iron, Total (0.012 to 1.800 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. TPTZ Iron Reagent Powder Pillows (for 10-mL sample) 100/pkg 26087-99 ® TPTZ Low Range Iron Reagent AccuVac Ampuls 25/pkg 25100-25 Required Apparatus (Powder Pillows) Description Quantity/Test Unit...
Page 314 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 315: Powder Pillows
Iron, Total FerroMo Method Method 8365 Powder Pillows (0.01 to 1.80 mg/L) Scope and Application: For cooling water containing molybdate-based treatment Adapted from G. Frederick Smith Chemical Co., The Iron Reagents, 3rd ed. (1980) Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 316 Iron, Total (0.01 to 1.80 mg/L) 5. Invert several times to 6. Fill a clean, 25-mL 7. Developed Sample: 8. Press TIMER>OK dissolve the reagents. graduated cylinder to the Add the contents of one A three-minute reaction 25-mL mark with prepared FerroMo Iron Reagent 2 period will begin.
Page 317 Iron, Total (0.01 to 1.80 mg/L) Sample Collection, Storage, and Preservation Collect samples in acid-washed glass or plastic bottles. To preserve samples, adjust the sample pH to 2 or less with hydrochloric acid (about 2 mL per liter)*. Samples preserved in this manner can be stored up to six months at room temperature.
Page 318 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 319 Iron, Total ® FerroVer Method Method 8008 ® Powder Pillows or AccuVac Ampuls (0.02 to 3.00 mg/L) Scope and Application: For water, wastewater, and seawater; digestion is required for determining total iron; USEPA approved for reporting wastewater analysis Adapted from Standard Methods for the Examination of Water and Wastewater Federal Register, June 27, 1980;...
Page 320 Iron, Total (0.02 to 3.00 mg/L) 5. Press > 6. Blank Preparation: 7. When the timer 8. Place the prepared TIMER expires, insert the blank sample into the cell holder A three-minute reaction Fill a second square into the cell holder with the with the fill line facing right.
Page 321 Iron, Total (0.02 to 3.00 mg/L) 5. Quickly invert the 6. Press > 7. When the timer 8. Insert the AccuVac TIMER Ampul several times to expires, insert the blank Ampul into the cell holder. A three-minute reaction mix. into the cell holder. period will begin.
Page 322: Standard Additions
Iron, Total (0.02 to 3.00 mg/L) Sample Collection, Storage, and Preservation Collect samples in acid-cleaned glass or plastic containers. No acid addition is necessary if analyzing the sample immediately. To preserve samples, adjust the pH to 2 or less with concentrated nitric acid (about 2 mL per liter).
Page 323 Iron, Total (0.02 to 3.00 mg/L) Standard Solution Method 1. Prepare a 2.00-mg/L Fe standard solution by pipetting 2.00 mL of Iron Standard Solution, 100-mg/L, into a 100-mL volumetric flask. Dilute to the mark with deionized water. Stopper and invert to mix. Prepare this solution daily. Perform the iron procedure as described above.
Page 324 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 325 Lead ®1 LeadTrak Fast Column Extraction Method Method 8317 (5 to 150 µg/L) Scope and Application: For drinking water Patent Number 5,019,516 Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water instead of the sample.
Page 326: Stored Programs
Lead (5 to 150 µg/L) Fast Column Extraction Method 8317 1. Press 2. Select the test. 3. Fill a 100-mL plastic 4. Using a plastic 1-mL graduated cylinder with dropper, add 1.0 mL of STORED PROGRAMS 100 mL of the sample. pPb-1 Acid Preservative Pour the measured Solution to the sample and...
Page 327 Lead (5 to 150 µg/L) 9. Pour the prepared 10. After the flow has 11. Place a clean, dry 12. Allow the Eluant sample slowly into the stopped, fully compress 150-mL beaker under the Solution to drip slowly center of the Column the absorbent pad in the Extractor.
Page 328 Lead (5 to 150 µg/L) 17. When the timer 18. Press 19. Remove the sample 20. Insert the sample cell ZERO expires, insert the sample cell and add 3 drops of into the cell holder with the The display will show: cell into the cell holder with pPb-6 Decolorizer fill line facing right.
Page 329 Lead (5 to 150 µg/L) Apparatus and Sample Preparation Because lead is very common to our environment, care must be taken to prevent sample contamination. Follow these steps for greatest test accuracy: • Lead-free water is necessary to minimize sample contamination when rinsing apparatus or diluting sample.
Page 330 Lead (5 to 150 µg/L) • Cap and invert several times to mix. • After two minutes the sample is ready for analysis. Steps are skipped in the analysis procedure. Use 100 mL of this preserved sample directly in step 6. •...
Page 331 Lead (5 to 150 µg/L) Standard Solution Method Using Class A glassware, prepare a 10-mg/L lead working standard solution by pipetting 1.0 mL of Lead Standard Solution, 1000-mg/L, into a 100-mL volumetric flask. Use a ® TenSette Pipet to add 0.2 mL of concentrated nitric acid to the flask. Dilute to the mark with lead-free deionized water.
Page 332 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 333 Lead Dithizone Method Method 8033 Powder Pillows (3 to 300 µg/L) Scope and Application: For water and wastewater; USEPA accepted for reporting for wastewater analysis (digestion is required). Adapted from Snyder, L. J., Analytical Chemistry, 19 684 (1947). Procedure is equivalent to Standard Method 3500-Pb D for wastewater analysis. Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 334: Powder Pillows
Lead (3 to 300 µg/L) Powder Pillows Method 8033 DANGER Cyanide is a deadly poison. Use a fume hood. Maintain cyanide solutions at pH 11 or greater to prevent formation of cyanide gas. 1. Press Select the test. 3. Fill a 250-mL 4.
Page 335 Lead (3 to 300 µg/L) 9. Stopper. Invert. Open 10. Continue adding 5.0 N 11. Add 5 more drops of 12. Add 2 heaping 1.0-g stopcock to vent. Close Sodium Hydroxide 5.0 N Sodium Hydroxide scoops of potassium the stopcock and shake Standard Solution Standard Solution.
Page 336 Lead (3 to 300 µg/L) 17. Press 18. Insert the prepared 19. Press ZERO READ sample into the cell holder The display will show: Results are in µg/L Pb with the fill line facing right. 0 µg/L Pb Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Highly buffered samples or...
Page 337 Lead (3 to 300 µg/L) 2. Repeat extraction with fresh 5-mL portions of prepared dithizone solution (collecting the bottom layer each time in appropriate waste collection vessel) until the bottom layer shows a pure dark green color for three successive extracts. Extractions can be repeated a number of times without appreciably affecting the amount of lead in the sample.
Page 338 Lead (3 to 300 µg/L) Standard Solution Method 1. Prepare a 10-mg/L lead standard solution by pipetting 10.00 mL of Lead Standard Solution, 100-mg/L, into a 100-mL volumetric flask. 2. Add 0.2 mL of concentrated nitric acid using a TenSette Pipet to prevent the adsorption of lead onto the container walls.
Page 339 Lead (3 to 300 µg/L) Required Apparatus (continued) Description Quantity/Test Unit Cat. No. Spoon, measuring,1-g each 510-00 Support Ring, 4" each 580-01 Support Ring Stand, 5" x 8" base each 563-00 Recommended Standards Description Unit Cat. No. Lead Standard Solution, 100 mg/L Pb 100 mL 12617-42 Lead Standard Solution, 10-mL Voluette Ampules, 50-mg/L Pb...
Page 340 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 341 Lead PAR Method Method 10216 TNTplus™ 850 (0.1 to 2.0 mg/L Pb) Scope and Application: For wastewater and process control Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on package.
Page 342 Lead (0.1 to 2.0 mg/L Pb) TNTplus Method 10216 Ø HRS MIN SEC HRS MIN SEC 1. Pipet 10 mL of sample Add 1 level spoonful 3. Cap the reaction tube 4. Wait two minutes. into the 20-mm reaction of Reagent A to the and invert 2–3 times.
Page 343: Reagent Blank
Lead (0.1 to 2.0 mg/L Pb) Reagent Blank A reagent blank can be measured, and the value subtracted from the results of each test performed using the same reagent lot number. Use deionized water in place of sample and run the procedure as described.
Page 344 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 345 Manganese 1-(2-Pyridylazo)-2-Naphthol PAN Method Method 8149 Powder Pillows LR (0.006 to 0.700 mg/L) Scope and Application: For water and wastewater; digestion is required for determining total manganese Adapted from Goto, K., et al., Talanta, 24, 652-3 (1977) Test Preparation Before starting the test: Rinse all glassware with 1:1 Nitric Acid Solution.
Page 346 Manganese LR (0.006 to 0.700 mg/L) 5. Add the contents of 6. Add 12 drops of 7. Add 12 drops of PAN 8. Press TIMER>OK one Ascorbic Acid Powder Alkaline-Cyanide Reagent Indicator Solution, 0.1%, A two-minute reaction Pillow to each cell. Solution to each cell.
Page 347 Manganese LR (0.006 to 0.700 mg/L) Sample Collection, Storage, and Preservation Collect samples in a clean plastic container. Adjust the pH to 2 or less with Concentrated Nitric Acid* (about 2 mL per liter). Preserved samples can be stored up to six months at room temperature.
Page 348 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 349 Manganese Periodate Oxidation Method Method 8034 Powder Pillows HR (0.1 to 20.0 mg/L) Scope and Application: For soluble manganese in water and wastewater; USEPA approved for reporting wastewater analyses (digestion required) Adapted from Standard Methods for the Examination of Water and Wastewater Federal Register, 44(116) 34193 (June 14, 1979) Test Preparation Before starting the test:...
Page 350 Manganese HR (0.1 to 20.0 mg/L) 5. Add the contents of 6. Press 7. Blank Preparation: 8. When the timer TIMER>OK one Sodium Periodate Fill a second square expires, insert the blank A two-minute reaction Powder Pillow to the sample cell with 10 mL of into the cell holder with the period will begin.
Page 351 Manganese HR (0.1 to 20.0 mg/L) Sample Collection, Storage, and Preservation Collect samples in acid-washed plastic bottles. Do not use glass containers due to possible adsorption of Mn to glass. If samples are acidified, adjust the pH to 4–5 with 5.0 N Sodium Hydroxide* before analysis.
Page 352 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 353 Mercury Cold Vapor Mercury Concentration Method Method 10065 (0.1 to 2.5 µg/L) Scope and Application: For water, wastewater, and seawater Patent no. 5,733,786 Test Preparation Before starting the test: Perform phase 1 of the procedure in a fume hood. Toxic chlorine or other gases may be produced. Use dedicated digestion glassware and sample cells for this procedure.
Page 354 Mercury (0.1 to 2.5 µg/L) 5. Add 7.5 g of 6. Cover the flask with a 7. Continue to stir and 8. Cool the digested potassium permanganate watch glass. Begin heating heat the sample at 90 °C sample to room to the sample.
Page 355 Mercury (0.1 to 2.5 µg/L) Phase 2: Cold Vapor Separation and Preconcentration of Mercury 1. Transfer the digested 2. Set the Gas Washing 3. Connect the 100-mL 4. Pipet 8 mL of sample to the Cold Vapor Bottle in the support ring. Erlenmeyer flask to the HgEx Reagent B into the Gas Washing Bottle.
Page 356 Mercury (0.1 to 2.5 µg/L) 9. Connect the Mercury 10. Shake an ampule of 11. Stopper the side neck 12. Reconnect the Absorber column to the HgEx Reagent A to on the Glass Washing vacuum to the Mercury Gas Washing Bottle using suspend undissolved Bottle.
Page 357 Mercury (0.1 to 2.5 µg/L) 17. Turn off or disconnect 18. Remove the distilling 19. Pipet 3 mL of HgEx power to the vacuum Receiver from the Mercury Reagent B into the pump when the volume in Absorber Column. Mercury Absorber Column the Distilling Receiver Reconnect the 100-mL without applying vacuum.
Page 358 Mercury (0.1 to 2.5 µg/L) 5. During the reaction 6. Wipe the sample cell. 7. Remove the cell from 8. Return the sample cell period, transfer the After the timer expires, the cell holder. Add the to the cell holder with the solution to a sample cell.
Page 359 Mercury (0.1 to 2.5 µg/L) Table 1 Interfering Substances and Levels (continued) Ion or Substance Concentration – – –N 50 mg/L NO –N 10 mg/L Pb 100 mg/L SiO 10 mg/L Zn Sample Collection and Preservation Collect 1000 mL of sample in an analytically clean, glass or polyethylene terephthalate (PET) container.
Page 360: System Start-Up
Mercury (0.1 to 2.5 µg/L) 4. Pipet 10.0 mL of the 0.1-mg/L mercury standard solution into the Gas Washing Bottle. Swirl to mix. 5. Begin at step of Phase 2. Follow the procedure steps. 6. Test the eluate as described in Phase 3. The displayed concentration should be 0.9–1.1 µg/L Hg.
Page 361 Mercury (0.1 to 2.5 µg/L) Glassware Care Use of dedicated glassware and sample cells is recommended because of the sensitivity of this procedure. Thoroughly clean the glassware and sample cells between tests. After washing, rinse with 1:1 hydrochloric acid solution, then rinse several times with deionized water.
Page 362 Mercury (0.1 to 2.5 µg/L) Pollution Prevention and Waste Management Proper management and disposal of waste is the responsibility of the waste generator. It is up to the generator to arrange for proper disposal and comply with applicable local, state, and federal regulations governing waste disposal.
Page 363 Mercury (0.1 to 2.5 µg/L) Required Apparatus (continued) Description Quantity/Test Unit Cat. No. Distilling Receiver, 10-mL each 26554-38 Flask, Erlenmeyer, 100-mL each 26553-42 Funnel, micro each 25843-35 Gas Washing Bottle, 1200-mL each 26622-00 Glass Elbow, 90-degree, with hose adapter each 26552-00 Mercury Absorber Column each...
Page 364 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 365: Molybdenum, Molybdate
Molybdenum, Molybdate Mercaptoacetic Acid Method Method 8036 ® Powder Pillows or AccuVac Ampuls HR (0.2 to 40.0 mg/L) Scope and Application: For water and wastewater. Adapted from Analytical Chemistry, 25(9) 1363 (1953) Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water instead of the sample.
Page 366 Molybdenum, Molybdate HR (0.2 to 40.0 mg/L) 5. Add the contents of 6. Add the contents of 7. Press 8. Blank Preparation: TIMER>OK one MolyVer 2 Reagent one MolyVer 3 Reagent When the timer expires, fill A five-minute reaction Powder Pillow. Swirl Powder Pillow.
Page 367 Molybdenum, Molybdate HR (0.2 to 40.0 mg/L) 5. Fill a MolyVer 6 6. Invert the Ampul 7. Press 8. When the timer TIMER>OK ® AccuVac Ampul with the several times to mix. expires, insert the blank A five-minute reaction treated sample. into the cell holder.
Page 368 Molybdenum, Molybdate HR (0.2 to 40.0 mg/L) Sample Collection, Storage, and Preservation Collect samples in clean plastic or glass bottles. Adjust the pH to 2 or less with nitric acid (about 2 mL/L). Preserved samples can be stored up to 6 months at room temperature. Adjust the pH to 7 with 5.0 N Sodium Hydroxide before analysis.
Page 369 Molybdenum, Molybdate HR (0.2 to 40.0 mg/L) Standard Solution Method To assure the accuracy of the test, use a Molybdenum Standard Solution, 10.0-mg/L Mo . Follow the procedure for powder pillows or AccuVac Ampuls. Standard Adjust 1. Use a Molybdenum Standard Solution, 10.0 mg/L Mo to perform the molybdenum procedure as described above.
Page 370 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 371 Molybdenum, Molybdate Ternary Complex Method Method 8169 Powder Pillows (0.02 to 3.00 mg/L) Scope and Application: For boiler and cooling tower waters Test Preparation Before starting the test: Analyze samples immediately after collection. Filter turbid samples using filter paper and a funnel Optional Reagents and Apparatus on page Collect the following items: Quantity...
Page 372 Molybdenum, Molybdate (0.02 to 3.00 mg/L) 5. Prepared Sample: 6. Pour 10-mL of the 7. Developed Sample 8. Press TIMER>OK Stopper the cylinder and prepared sample into a Add 0.5 mL of A two-minute reaction shake to dissolve the square sample cell. Molybdenum 2 Reagent to period will begin.
Page 373 Molybdenum, Molybdate (0.02 to 3.00 mg/L) Table 1 Substances That Cause a Negative Interference (continued) Interfering Substance Interference Levels and Treatments Copper Greater than 98 mg/L Diethanoldithiocarbamate Greater than 32 mg/L EDTA Greater than 1500 mg/L Ethylene Glycol Greater than 2% (by volume) Iron Greater than 200 mg/L Lignin Sulfonate...
Page 374 Molybdenum, Molybdate (0.02 to 3.00 mg/L) Sample Collection, Storage, and Preservation Collect samples in glass or plastic bottles. Analyze samples immediately. Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify the chemical form.
Page 375 Molybdenum, Molybdate (0.02 to 3.00 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Molybdenum Reagent Set for 20-mL sample (100 tests), includes: — — 24494-00 (1) Molybdenum 1 Reagent (LR) Molybdate Powder Pillows 100/pkg 23524-49 (1) Molybdenum 2 Reagent Solution 0.5 mL 50 mL MDB 23525-12...
Page 376 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 377 Nickel 1-(2 Pyridylazo)-2-Napthol (PAN) Method Method 8150 Powder Pillows (0.006 to 1.000 mg/L) Scope and Application: For water and wastewater; digestion is required for determining total nickel Adapted from Watanabe, H., Talanta, 21 295 (1974) Test Preparation Before starting the test: Cobalt concentration can be determined with the same sample by using Program Number 110.
Page 378 Nickel (0.006 to 1.000 mg/L) 5. Add the contents of 6. Stopper the cells. 7. Using the provided 8. Stopper the cells. one Phthalate-Phosphate Immediately shake to plastic dropper, add Invert several times to mix. Reagent Powder Pillow to dissolve. 0.5 mL of 0.3% PAN each cell.
Page 379 Nickel (0.006 to 1.000 mg/L) 13. Press 14. Wipe the sample cell 15. Press . The ZERO READ and insert it into the cell instrument will read the The display will show: holder with the fill line sample at 560 and 0.000 mg/L Ni facing right.
Page 380 Nickel (0.006 to 1.000 mg/L) Sample Collection, Storage, and Preservation Collect samples in acid-washed plastic bottles. Adjust the sample pH to 2 or less with Nitric Acid*, about 5 mL per liter. Preserved samples can be stored up to six months at room temperature.
Page 381 Nickel (0.006 to 1.000 mg/L) Summary of Method After buffering the sample and masking any Fe with pyrophosphate, the nickel is reacted with 1-(2-Pyridylazo)-2-Naphthol indicator. The indicator forms complexes with most metals present. After color development, EDTA is added to destroy all metal-PAN complexes except nickel and cobalt.
Page 382 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 383 Nickel Heptoxime Method Method 8037 Powder Pillows (0.02 to 1.80 mg/L Ni) Scope and Application: For water, wastewater, and seawater; USEPA accepted for reporting wastewater analyses (digestion required) Adapted from Chemie Analytique, 36 43 (1954) Procedure is equivalent to Standard Method 3500-Ni D for wastewater Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 384: Stored Programs
Nickel (0.02 to 1.80 mg/L Ni) Powder Pillows Method 8037 1. Press 2. Select the test. 3. Measure 300 mL of 4. Add the contents of sample in a 500-mL one Nickel 1 Reagent STORED PROGRAMS graduated cylinder. Pour Powder Pillow to the into a 500-mL separatory funnel.
Page 385 Nickel (0.02 to 1.80 mg/L Ni) HRS MIN SEC HRS MIN SEC Repeat Steps 8—11 9. Close the stopcock 10. Press 11. Prepared Sample: 12. Repeat steps TIMER>OK and invert for 30 seconds. When the timer expires, through two additional A third five-minute reaction wait for the layers to times with 10-mL volumes...
Page 386 Nickel (0.02 to 1.80 mg/L Ni) Interferences Cobalt, copper, and iron interferences can be overcome by adding additional Nickel 1 Reagent Powder Pillows in step 4. The tolerance limits of these interferences are shown in Table Table 1 Interfering Substances Tolerance Limit (mg/L): Pillows of Nickel 1 Reagent Cobalt...
Page 387 Nickel (0.02 to 1.80 mg/L Ni) Standard Solutions Method 1. Prepare a 10.0-mg/L nickel working standard solution by pipetting 10.0 mL of a Nickel Standard Solution, 1000-mg/L, into a 1000-mL volumetric flask. Dilute to the mark with deionized water. Prepare this solution daily. Prepare a 1.0-mg/L nickel standard solution by diluting 50.0 mL of the 10-mg/L working standard solution to 500 mL in a volumetric flask.
Page 388 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 389 Nickel Dimethylglyoxime Method Method 10220 TNTplus™ 856 (0.1 to 6.0 mg/L Ni) Scope and Application: For wastewater, drinking water and process analysis. Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on package.
Page 390: Reagent Blank
Nickel (0.1 to 6.0 mg/L Ni) TNTplus Method 10220 1. Pipet 2.0 mL of Cap the vial and 3. Pipet 0.2 mL (200 µL) 4. Cap and invert the vial sample into the vial. invert 2–3 times until the of Solution A into the vial. 2–3 times.
Page 391 Nickel (0.1 to 6.0 mg/L Ni) Sample Blanks Colored or turbid samples can cause high results. To compensate for color or turbidity the procedure is repeated and the color forming reagent that is present in Solution A is not added. To determine the sample blank run the procedure as given, but substitute 0.2 mL of deionized water in place of the 0.2 mL of Solution A in step 3.
Page 392 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 393 Nitrate Cadmium Reduction Method Method 8039 ® – Powder Pillows or AccuVac Ampuls HR (0.3 to 30.0 mg/L NO –N) Scope and Application: For water, wastewater, and seawater. Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water instead of the sample.
Page 394 – Nitrate HR (0.3 to 30.0 mg/L NO –N) 5. Press 6. Shake the cell 7. When the timer 8. Blank Preparation: TIMER>OK vigorously until the timer expires, press When the timer expires, fill TIMER>OK A one-minute reaction expires. again. A five-minute a second square sample period will begin.
Page 395 – Nitrate HR (0.3 to 30.0 mg/L NO –N) ® AccuVac Ampul Method 8039 1. Select the test 2. Insert Adapter C. 3. Prepared Sample: 4. Tap the bottom of a Collect at least 40 mL of NitraVer 5 Nitrate ®...
Page 396 – Nitrate HR (0.3 to 30.0 mg/L NO –N) 9. Wipe the blank and 10. Press 11. Within one minute 12. Press ZERO READ insert it into the cell holder. after the timer expires, The display will show: Results are in mg/L wipe the Ampul and insert –...
Page 397 – Nitrate HR (0.3 to 30.0 mg/L NO –N) Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify the chemical form. 2. Press . Press . A summary of the standard OPTIONS>MORE STANDARD ADDITIONS additions procedure will appear.
Page 398 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 399 Nitrate Cadmium Reduction Method Method 8192 – Powder Pillows LR (0.01 to 0.50 mg/L NO –N) Scope and Application: For water, wastewater, and seawater Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample.
Page 400 – Nitrate LR (0.01 to 0.50 mg/L NO –N) 5. Press 6. Shake the cylinder 7. When the timer 8. When the timer TIMER>OK vigorously during the expires, press expires, carefully pour TIMER>OK A 3-minute reaction time three-minute timer. again. 10 mL of the sample into a will begin.
Page 401 – Nitrate LR (0.01 to 0.50 mg/L NO –N) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Calcium 100 mg/L Chloride concentrations above 100 mg/L will cause low results. The test may be used at high Chloride chloride concentrations (seawater) but a calibration must be done using standards spiked to the same chloride concentration.
Page 402 – Nitrate LR (0.01 to 0.50 mg/L NO –N) 5. Prepare three sample spikes. Fill three mixing cylinders* with 15 mL of sample. Use the ® TenSette Pipet to add 0.1 mL, 0.2 mL, and 0.3 mL of standard, respectively, to each sample and mix thoroughly.
Page 403 – Nitrate LR (0.01 to 0.50 mg/L NO –N) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Low Range Nitrate Reagent Set (100 tests), includes: — — 24298-00 ® NitraVer 6 Nitrate Reagent Powder Pillows 100/pkg 21072-49 ®...
Page 404 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 405 Nitrate Chromotropic Acid Method Method 10020 – Test ‘N Tube™ Vials HR (0.2 to 30.0 mg/L NO –N) Scope and Application: For water and wastewater Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 406 – Nitrate HR (0.2 to 30.0 mg/L NO –N) 5. Press 6. Prepared Sample: 7. Cap and invert ten 8. Press ZERO TIMER>OK Remove the vial from the times to mix. The display will show: A five-minute reaction instrument. Using a funnel, Some solid matter will period will begin.
Page 407: Sample Collection, Preservation, And Storage
– Nitrate HR (0.2 to 30.0 mg/L NO –N) Sample Collection, Preservation, and Storage Collect samples in clean plastic or glass bottles. Store at 4 °C (39 °F) or lower if the sample is to be analyzed within 24 to 48 hours. Warm to room temperature before running the test. For longer storage periods (up to 14 days), adjust sample pH to 2 or less with Concentrated Sulfuric Acid, ACS* (about 2 mL per liter).
Page 408 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 409 Nitrate Dimethylphenol Method Method 10206 – TNTplus 836 HR (5–35 mg/L NO –N or 22–155 mg/L NO Scope and Application: For wastewater, drinking water, surface water, and process water Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on package.
Page 410: Reagent Blank
– Nitrate HR (5–35 mg/L NO –N or 22–155 mg/L NO 5. After the timer expires wipe the vial and insert it into the cell holder. The instrument reads the barcode, then selects and performs the correct test. No Zero is required. Results are in mg/L –N.
Page 411 – Nitrate HR (5–35 mg/L NO –N or 22–155 mg/L NO Interferences The items listed in Table 1 have been individually checked up to the given concentrations and do not cause interference. Cumulative effects or influence of other ions has not been determined.
Page 412 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 413 Nitrate Dimethylphenol Method Method 10206 TNTplus 835 – LR (0.23 to 13.50 mg/L NO –N or 1.00 to 60.00 mg/L NO Scope and Application: For wastewater, drinking water, surface water, and process water Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on package.
Page 414 – Nitrate LR (0.23 to 13.50 mg/L NO –N or 1.00 to 60.00 mg/L NO 5. After the timer expires wipe the vial and insert the prepared vial into the cell holder. The instrument reads the barcode, then selects and performs the correct test.
Page 415 – Nitrate LR (0.23 to 13.50 mg/L NO –N or 1.00 to 60.00 mg/L NO Interferences The items listed in Table 1 have been individually checked up to the given concentrations and do not cause interference. The cumulative effects and influence of other ions has not been determined.
Page 416 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 417: Powder Pillows
Nitrate Cadmium Reduction Method Method 8171 ® – Powder Pillows or AccuVac Ampuls MR (0.1 to 10.0 mg/L NO –N) Scope and Application: For water, wastewater, and seawater Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water instead of the sample.
Page 418 – Nitrate MR (0.1 to 10.0 mg/L NO –N) 5. Press 6. When the timer 7. Blank Preparation: 8. Insert the blank into TIMER>OK expires, press When the timer expires, fill the cell holder with the fill TIMER>OK A one-minute reaction a second square sample line facing right.
Page 419 – Nitrate MR (0.1 to 10.0 mg/L NO –N) 5. Press 6. Invert the Ampul 7. When the timer 8. Blank Preparation: TIMER>OK 48–52 times as the timer expires, press When the timer expires, fill TIMER>OK A one-minute reaction counts down. a round sample cell with period will begin.
Page 420 – Nitrate MR (0.1 to 10.0 mg/L NO –N) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Chloride concentrations above 100 mg/L will cause low results. The test may be used at high Chloride chloride concentrations (seawater) but a calibration must be done using standards spiked to the same chloride concentration.
Page 421 – Nitrate MR (0.1 to 10.0 mg/L NO –N) 5. Prepare three sample spikes. Fill three sample cells with 10 mL of sample. Use the ® TenSette Pipet to add 0.1 mL, 0.2 mL, and 0.3 mL of standard, respectively, to each sample and mix thoroughly.
Page 422 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 423 Nitrite Diazotization Method Method 10019 – Test ‘N Tube™ Vials LR (0.003 to 0.500 mg/L NO –N) Scope and Application: For water, wastewater, and seawater Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 424 – Nitrite LR (0.003 to 0.500 mg/L NO –N) 5. Blank Preparation: 6. Wipe the blank and 7. Press 8. Insert the prepared ZERO When the timer expires, fill insert it into the 16-mm sample cell into the The display will show: an empty Test ‘N Tube™...
Page 425 – Nitrite LR (0.003 to 0.500 mg/L NO –N) Accuracy Check Standard Solution Method Preparing nitrite standards is difficult. Use the standard preparation instructions in Standard Methods for the Examination of Water and Wastewater, Method 4500-NO2 B. Prepare a 0.300-mg/L standard. Perform the nitrite test on the standard solution. Summary of Method Nitrite in the sample reacts with sulfanilic acid to form an intermediate diazonium salt.
Page 426 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 427 Nitrite Diazotization Method Method 8507 – ® Powder Pillows or AccuVac Ampuls LR (0.002 to 0.300 mg/L NO –N) Scope and Application: For water, wastewater, and seawater; USEPA approved for wastewater analysis Federal Register, 44(85), 25505 (May 1, 1979) Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 428 – Nitrite LR (0.002 to 0.300 mg/L NO –N) 5. Press 6. Blank Preparation: 7. Wipe the blank and 8. Wipe the prepared TIMER>OK When the timer expires, fill insert it into the cell holder sample and insert it into A 20-minute reaction a second square sample with the fill line facing right.
Page 429 – Nitrite LR (0.002 to 0.300 mg/L NO –N) 5. Press . Press 6. Blank Preparation: 7. Wipe the blank and 8. Wipe the AccuVac TIMER . A 20-minute reaction When the timer expires, fill insert it into the cell holder. Ampul and insert it into the period will begin.
Page 430 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 431 Nitrite Ferrous Sulfate Method Method 8153 – Powder Pillows HR (2 to 250 mg/L NO Scope and Application: For cooling tower waters Adapted from McAlpine, R. and Soule, B., Qualitative Chemical Analysis, New York, 476, 575 (1933) Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 432 – Nitrite HR (2 to 250 mg/L NO 5. Press 6. Blank Preparation: 7. Wipe the blank and 8. Press TIMER>OK ZERO Fill a second square insert it into the cell holder A ten-minute reaction The display will show: sample cell with 10 mL of will the fill line facing right.
Page 433 – Nitrite HR (2 to 250 mg/L NO Accuracy Check Standard Solution Method Preparing nitrite standards is difficult. Use the standard preparation instructions in Standard Methods for the Examination of Water and Wastewater. Prepare a 200-mg/L standard using Sodium Nitrite, ACS*, reagent grade. Summary of Method The method uses ferrous sulfate in an acidic medium to reduce nitrite to nitrous oxide.
Page 434 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 435: Diazotization Method
Nitrite Diazotization Method Method 10207 TNTplus™ 839 – LR (0.015 to 0.600 mg/L NO –N or 0.05 to 2.00 mg/L NO Scope and Application: For wastewater, drinking water, surface water and mineral water Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on the reagent package.
Page 436 – Nitrite LR (0.015 to 0.600 mg/L NO –N or 0.05 to 2.00 mg/L NO HRS MIN SEC HRS MIN SEC 5. Wait 10 minutes. 6. After 10 minutes, 7. Insert the prepared Note: Press thoroughly clean the vial into the cell holder. OPTIONS>MORE>...
Page 437 – Nitrite LR (0.015 to 0.600 mg/L NO –N or 0.05 to 2.00 mg/L NO Interferences The ions listed in Table 1 have been individually tested up to the given concentrations and do not cause interference. The cumulative effects of these ions or the influence of other ions have not been determined.
Page 438 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 439: Nitrogen, Ammonia
Nitrogen, Ammonia Salicylate Method Method 8155 Powder Pillows (0.01 to 0.50 mg/L NH –N) Scope and Application: For water, wastewater, and seawater Adapted from Clin. Chim. Acta., 14, 403 (1966) Test Preparation Collect the following items: Quantity Ammonia Cyanurate Reagent pillows Ammonia Salicylate Reagent pillows Sample Cells, 1-inch square, 10-mL Note: Reorder information for consumables and replacement items is on page 5.
Page 440 Nitrogen, Ammonia (0.01 to 0.50 mg/L NH –N) 4. Add the contents of 5. Press > 6. When the timer 7. Press TIMER TIMER>OK one Ammonia Salicylate three-minute reaction expires, add the contents 15-minute reaction period Powder Pillow to each cell. period will begin.
Page 441 Nitrogen, Ammonia (0.01 to 0.50 mg/L NH –N) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Calcium Greater than 1000 mg/L as CaCO All levels. Correct for iron interference as follows: Determine the amount of iron present in the sample by following one of the Iron, Total, procedures.
Page 442 Nitrogen, Ammonia (0.01 to 0.50 mg/L NH –N) 4. Open an Ammonia Nitrogen Standard Solution, 10-mg/L as NH –N. 5. Prepare three sample spikes. Fill three mixing cylinders with 25 mL of sample.Use the ® TenSette Pipet to add 0.2 mL, 0.4 mL, and 0.6 mL of standard, respectively to the cylinders and mix each thoroughly.
Page 443 Nitrogen, Ammonia (0.01 to 0.50 mg/L NH –N) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Ammonia Nitrogen Reagent Set for 10-mL samples (100 tests), includes: — — 26680-00 Includes: (2) Ammonia Cyanurate Reagent Powder Pillows 100/pkg 26531-99 (2) Ammonia Salicylate Reagent Powder Pillows 100/pkg...
Page 444 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 445 Nitrogen, Ammonia Nessler Method Method 8038 (0.02 to 2.50 mg/L NH –N) Scope and Application: For water, wastewater, and seawater; distillation is required for wastewater and seawater; USEPA accepted for wastewater analysis (distillation required); see Distillation on page 4 of this procedure. Adapted from Standard Methods for the Examination of Water and Wastewater 4500-NH B &...
Page 446 Nitrogen, Ammonia (0.02 to 2.50 mg/L NH –N) 5. Add three drops 6. Add three drops of 7. Pipet 1.0 mL of 8. Press TIMER>OK of Mineral Stabilizer to Polyvinyl Alcohol Nessler Reagent into each A one-minute reaction each cylinder. Stopper and Dispersing Agent to each cylinder.
Page 447 Nitrogen, Ammonia (0.02 to 2.50 mg/L NH –N) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Glycine, various aliphatic and aromatic amines, organic May cause greenish or other off colors or turbidity. Distill the sample if these compounds chloramines, acetone, are present.
Page 448 Nitrogen, Ammonia (0.02 to 2.50 mg/L NH –N) 2. To adjust the calibration curve using the reading obtained with the standard solution, press on the current program menu. Press OPTIONS>MORE STANDARD ADJUST 3. Press . Press to accept the displayed concentration. If an alternate ADJUST concentration is used, press the number in the box to enter the actual concentration, then press...
Page 449 Nitrogen, Ammonia (0.02 to 2.50 mg/L NH –N) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Ammonia Nitrogen Reagent Set, includes: — — 24582-00 Nessler Reagent 2 mL 500 mL 21194-49 Mineral Stabilizer 6 drops 50 mL SCDB 23766-26 Polyvinyl Alcohol Dispersing Agent 6 drops...
Page 450 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 451 Nitrogen, Ammonia Salicylate Method Method 10031 ™ Test ‘N Tube Vials HR (0.4 to 50.0 mg/L NH –N) Scope and Application: For water, wastewater, and seawater Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Small sample sizes (such as 0.1 mL) may not be representative of the entire sample.
Page 452 Nitrogen, Ammonia HR (0.4 to 50.0 mg/L NH –N) 5. Add the contents of 6. Cap the vials tightly 7. Press 8. After the waiting TIMER>OK one Ammonia Cyanurate and shake thoroughly to 20-minute reaction period period, wipe the blank and Reagent Powder Pillow to dissolve the powder.
Page 453 Nitrogen, Ammonia HR (0.4 to 50.0 mg/L NH –N) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Nitrite 600 mg/L as NO – –N Nitrate 5000 mg/L as NO – –N 3– Orthophosphate 5000 mg/L as PO –P 2–...
Page 454 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 455 Nitrogen, Ammonia Salicylate Method Method 10023 Test ‘N Tube™ Vials LR (0.02 to 2.50 mg/L NH –N) Scope and Application: For water, wastewater, and seawater Adapted from Clin. Chim. Acta, 14, 403 (1966) Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Good safety habits and laboratory techniques should be used throughout the procedure.
Page 456 Nitrogen, Ammonia LR (0.02 to 2.50 mg/L NH –N) 5. Add the contents of 6. Cap the vials tightly 7. Press 8. After the waiting TIMER>OK one Ammonia Cyanurate and shake thoroughly to 20-minute reaction period period, wipe the blank and Reagent Powder Pillow to dissolve the powder.
Page 457 Nitrogen, Ammonia LR (0.02 to 2.50 mg/L NH –N) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Measure about 350 mL of sample in a 500-mL Erlenmeyer flask. Add the contents of one Sulfide Inhibitor Reagent Powder Pillow .
Page 458 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 459 Nitrogen, Ammonia Salicylate Method Method 10205 TNTplus™ 830 ULR (0.015 to 2.000 mg/L NH –N) Scope and Application: For municipal and industrial wastewaters, environmental waters, and watershed protection monitoring. Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on package.
Page 460 Nitrogen, Ammonia ULR (0.015 to 2.000 mg/L NH –N) HRS MIN SEC HRS MIN SEC 5. Wait 15 minutes. 6. After 15 minutes, 7. Thoroughly clean the 8. Insert the prepared invert the sample an outside of the vial. vial into the cell holder. additional 2–3 times to Install the Light Shield in The instrument reads the...
Page 461 Measurement results can be verified using sample dilutions or standard additions. Samples with severe interferences require distillation. The manufacturer recommends the distillation procedure using the Hach General Purpose Distillation Set. Table 1 Interfering Substances and Levels Interfering Substance Interference Levels –...
Page 462 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 463 Nitrogen, Ammonia Salicylate Method Method 10205 TNTplus™ 831 LR (1 to 12 mg/L NH –N) Scope and Application: For surface waters, municipal and industrial wastewaters. Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on package.
Page 464 Nitrogen, Ammonia LR (1 to 12 mg/L NH –N) HRS MIN SEC HRS MIN SEC 5. Wait 15 minutes. 6. After 15 minutes, 7. Thoroughly clean the 8. Insert the prepared invert the sample an outside of the vial. vial into the cell holder. additional 2–3 times to Install the Light Shield in The instrument reads the...
Page 465 Measurement results can be verified using sample dilutions or standard additions. Samples with severe interferences require distillation. The manufacturer recommends the distillation procedure using the Hach General Purpose Distillation Set. Table 1 Interfering Substances and Levels Interfering Substance Interference Levels –...
Page 466 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 467 Nitrogen, Ammonia Salicylate Method Method 10205 TNTplus™ 832 HR (2 to 47 mg/L NH –N) Scope and Application: For surface waters, municipal and industrial wastewaters. Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on package.
Page 468 Nitrogen, Ammonia HR (2 to 47 mg/L NH –N) HRS MIN SEC HRS MIN SEC 5. Wait 15 minutes. 6. After 15 minutes, 7. Thoroughly clean the 8. Insert the prepared invert the sample an outside of the vial. vial into the cell holder. additional 2–3 times to Install the Light Shield in The instrument reads the...
Page 469 Measurement results can be verified using sample dilutions or standard additions. Samples with severe interferences require distillation. The manufacturer recommends the distillation procedure using the Hach General Purpose Distillation Set. Table 1 Interfering Substances and Levels Interfering Substance Interference Levels –...
Page 470 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 471 Nitrogen, Free Ammonia Indophenol Method Method 10201 (0.01 to 0.50 mg/L NH –N) Powder Pillows Scope and Application: For controlling free ammonia levels during the production of chloramines, at booster stations and for monitoring free ammonia levels in potable distribution system waters. U.S.
Page 472 Nitrogen, Free Ammonia (0.01 to 0.50 mg/L NH –N) 5. Cap the reagent bottle 6. Cap and invert the 7. Press 8. When the timer TIMER>OK to maintain reagent sample to mix. expires, add the contents A 5-minute reaction period performance and stability.
Page 473 Nitrogen, Free Ammonia (0.01 to 0.50 mg/L NH –N) 13. Insert the sample into 14. Press READ the cell holder with the Results are in mg/L 1-cm (flat) path in line with –N f. the indicator arrow on the adapter. Interferences This method is intended for finished, chloraminated drinking water samples that have a measurable combined (total) chlorine disinfectant residual.
Page 474: Sampling And Storage
Nitrogen, Free Ammonia (0.01 to 0.50 mg/L NH –N) 3. Cap the cell and invert until the reagent is dissolved. 4. Remove the cap. 5. Continue with the analysis at step using the pretreated sample as the sample cell. Color Development Time Test results are strongly influenced by sample temperature.
Page 475 Nitrogen, Free Ammonia (0.01 to 0.50 mg/L NH –N) 3. Press to accept the default values for standard concentrations, sample volume, and spike volumes. Press to change these values. After values are accepted, the EDIT unspiked sample reading will appear in the top row. See the user manual for more information.
Page 476 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 477: Nitrogen, Total
Nitrogen, Total Persulfate Digestion Method Method 10072 Test ‘N Tube™ Vials HR (2 to 150 mg/L N) Scope and Application: For water and wastewater Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Digestion is required for determining total nitrogen.
Page 478 Nitrogen, Total HR (2 to 150 mg/L N) Test ‘N Tube Method 10072 1. Turn on the DRB200 2. Using a funnel, add 3. Add 0.5 mL of sample 4. Cap both vials. Shake Reactor and heat to the contents of one Total to a vial (this is the vigorously for at least 105 °C.
Page 479 Nitrogen, Total HR (2 to 150 mg/L N) 9. Cap the tubes and 10. Press 11. After the timer expires, 12. Cap the tubes and TIMER>OK shake for 15 seconds. remove the caps from the shake for 15 seconds. The A three-minute reaction vials and add one TN reagent will not completely...
Page 480 Nitrogen, Total HR (2 to 150 mg/L N) Blanks for Colorimetric Measurement The reagent blank may be used up to seven days for measurements using the same lots of reagents. Store it in the dark at room temperature (18–25 °C). If a small amount of white floc appears prior to the end of one week, discard the reagent blank and prepare a new one.
Page 481 Nitrogen, Total HR (2 to 150 mg/L N) Sample Collection, Storage, and Preservation Collect samples in clean plastic or glass bottles. Best results are obtained with immediate analysis. Preserve the sample by reducing the pH to 2 or less with concentrated (at least 2 mL/L) Sulfuric Acid.
Page 482 Nitrogen, Total HR (2 to 150 mg/L N) 3. Press to accept the default values for standard concentration, sample volume, and spike volumes. Press to change these values. After values are accepted, the EDIT unspiked sample reading will appear in the top row. See the user manual for more information.
Page 483 Nitrogen, Total HR (2 to 150 mg/L N) Consumables and Replacement Items Required Reagents Description Unit Cat. No. Test ’N Tube™ Total HR Nitrogen Reagent Set 50 vials 27141-00 Required Apparatus Description Quantity/Test Unit Cat. No. DRB200 Reactor, 110 V, 15 x 16 mm each LTV082.53.40001 DRB200 Reactor, 220 V, 15 x 16 mm...
Page 484 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 485 Nitrogen, Total Persulfate Digestion Method Method 10071 Test ‘N Tube™ Vials LR (0.5 to 25.0 mg/L N) Scope and Application: For water and wastewater. Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Digestion is required for determining total nitrogen.
Page 486 Nitrogen, Total LR (0.5 to 25.0 mg/L N) Test ‘N Tube Method 10071 1. Turn on the DRB200 2. Using a funnel, add 3. Prepared Sample: 4. Cap both vials. Shake Reactor and heat to the contents of one Total Add 2 mL of sample to one vigorously for at least 105 °C.
Page 487 Nitrogen, Total LR (0.5 to 25.0 mg/L N) 9. Cap the tubes and 10. Press 11. After the timer expires, 12. Cap the tubes and TIMER>OK shake for 15 seconds. remove the caps from the shake for 15 seconds. The A three-minute reaction vials and add one TN reagent will not completely...
Page 488 Nitrogen, Total LR (0.5 to 25.0 mg/L N) Blanks for Colorimetric Measurement The reagent blank may be used up to seven days for measurements using the same lots of reagents. Store it in the dark at room temperature (18–25 °C). If a small amount of white floc appears prior to the end of one week, discard the reagent blank and prepare a new one.
Page 489 Nitrogen, Total LR (0.5 to 25.0 mg/L N) Sample Collection, Storage, and Preservation Collect samples in clean plastic or glass bottles. Best results are obtained with immediate analysis. Preserve the sample by reducing the pH to 2 or less with concentrated (at least 2 mL/L) Sulfuric Acid.
Page 490 Nitrogen, Total LR (0.5 to 25.0 mg/L N) 3. Press to accept the default values for standard concentration, sample volume, and spike volumes. Press to change these values. After values are accepted, the EDIT unspiked sample reading will appear in the top row. See the user manual for more information.
Page 491 Nitrogen, Total LR (0.5 to 25.0 mg/L N) Consumables and Replacement Items Required Reagents Description Unit Cat. No. Test ’N Tube™ Total Nitrogen Reagent Set 50 vials 26722-45 Required Apparatus Description Quantity/Test Unit Cat. No. DRB200 Reactor, 110 V, 15 x 16 mm each LTV082.53.40001 DRB200 Reactor, 220 V, 15 x 16 mm...
Page 492 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 493 Nitrogen, Total Persulfate Digestion Method Method 10208 TNTplus™ 826 LR (1 to 16 mg/L N) Scope and Application: For water and wastewater Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Read the Safety Advice and Expiration Date on the package.
Page 494 Nitrogen, Total LR (1 to 16 mg/L N) TNTplus Method 10208 1. Turn on the DRB200 2. Add 1.3 mL of 3. Insert the reaction 4. Remove the hot Reactor and heat to sample, 1.3 mL of tubes in the reactor. Heat reaction tubes from the 100 °C.
Page 495 Nitrogen, Total LR (1 to 16 mg/L N) Reagent Blanks A reagent blank can be measured and the value subtracted from the results of each test performed using the same reagent lot number. Use nitrogen-free deionized water in place of sample and run the procedure as described.
Page 496 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 497 Nitrogen, Total Persulfate Digestion Method Method 10208 TNTplus™ 827 HR (5 to 40 mg/L N) Scope and Application: For water and wastewater Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Read the Safety Advice and Expiration Date on the package.
Page 498 Nitrogen, Total HR (5 to 40 mg/L N) TNTplus Method 10208 1. Turn on the DRB200 2. Add 10.5 mL (500 µL) 3. Insert the reaction 4. Remove the hot Reactor and heat to of sample, 2.0 mL of tubes in the reactor. Heat reaction tubes from the 100 °C.
Page 499 Nitrogen, Total HR (5 to 40 mg/L N) Reagent Blanks A reagent blank can be measured and the value subtracted from the results of each test performed using the same reagent lot number. Use nitrogen-free deionized water in place of sample and run the procedure as described.
Page 500 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 501 Nitrogen, Total Persulfate Digestion Method Method 10208 TNTplus™ 828 UHR (20 to 100 mg/L N) Scope and Application: For water and wastewater Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Read the Safety Advice and Expiration Date on the package.
Page 502 Nitrogen, Total UHR (20 to 100 mg/L N) TNTplus Method 10208 1. Turn on the DRB200 2. Add 0.2 mL (200 µL) 3. Insert the reaction 4. Remove the hot Reactor and heat to of sample, 2.3 mL of tubes in the reactor. Heat reaction tubes from the 100 °C.
Page 503 Nitrogen, Total UHR (20 to 100 mg/L N) Reagent Blanks A reagent blank can be measured and the value subtracted from the results of each test performed using the same reagent lot number. Use nitrogen-free deionized water in place of sample and run the procedure as described.
Page 504 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 505: Nitrogen, Total Inorganic
Nitrogen, Total Inorganic Titanium Trichloride Reduction Method Method 10021 Test ‘N Tube™ Vials (0.2 to 25.0 mg/L N) Scope and Application: For water, wastewater, and seawater Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. For safety, wear gloves while breaking ampules.
Page 506 Nitrogen, Total Inorganic (0.2 to 25.0 mg/L N) 5. Pour the contents of 6. Immediately cap the 7. Place the vials in a 8. Press TIMER>OK one Total Inorganic vials. Shake gently for 30 centrifuge. A three-minute timer will Nitrogen Reductant seconds to mix the (If you do not have a begin.
Page 507 Nitrogen, Total Inorganic (0.2 to 25.0 mg/L N) 13. Press 14. When the timer 15. Press 16. Wipe the prepared TIMER>OK ZERO expires, wipe the blank sample and insert it into A 20-minute reaction The display will show: and insert it into the the 16-mm round cell period will begin.
Page 508: Sample Collection, Preservation, And Storage
Nitrogen, Total Inorganic (0.2 to 25.0 mg/L N) Sample Collection, Preservation, and Storage Collect samples in clean plastic or glass bottles. Best results are obtained with immediate analysis. If chlorine is known to be present, add one drop of 0.1 N Sodium Thiosulfate* for each 0.3 mg/L Cl in a one-liter sample.
Page 509 Nitrogen, Total Inorganic (0.2 to 25.0 mg/L N) Method Performance The total inorganic nitrogen test is designed to provide an estimate of the total nitrite, nitrate, and ammonia nitrogen load present in a water or wastewater sample. This test is most applicable to the monitoring of samples taken from an industrial process stream or a wastewater treatment stream where it is important to track the inorganic nitrogen load as it passes through the treatment process.
Page 510 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 511: Nitrogen, Total Kjeldahl
Scope and Application: For water, wastewater, and sludge; digestion is required. Adapted from Hach, et. al., Journal of Association of Official Analytical Chemists, 70(5) 783-787 (1987); Hach, et. al., Journal of Agricultural and Food Chemistry, 33(6) 1117-1123 (1985); Standard Methods for the Examination of Water and Wastewater...
Page 512: Stored Programs
Nitrogen, Total Kjeldahl (1 to 150 mg/L) Nessler Method 8075 1. Press 2. Select the test. 3. Prepared Sample: 4. Blank Preparation: Digest the sample amount Digest an equal amount of STORED PROGRAMS as described in the deionized water as the ®...
Page 513 Nitrogen, Total Kjeldahl (1 to 150 mg/L) 9. Fill both cylinders to 10. Add three drops of 11. Add three drops of 12. Fill both cylinders to the 20-mL mark with Mineral Stabilizer to each Polyvinyl Alcohol the 25-mL mark with deionized water.
Page 514 Nitrogen, Total Kjeldahl (1 to 150 mg/L) 17. Press . The 18. Wipe the prepared 19. Calculate sample TKN ZERO display will show: sample and insert it into as follows: × 0 mg/L TKN the cell holder with the fill 75 A ppm TKN ----------------...
Page 515 Nitrogen, Total Kjeldahl (1 to 150 mg/L) Sample Collection, Storage, and Preservation Collect samples in clean glass or plastic containers. Adjust the pH to 2 or less with Sulfuric Acid (about 2 mL per liter) and cool to 4 °C (39 °F). Preserved samples can be stored up to 28 days.
Page 516 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 517: Organic Carbon, Total
Organic Carbon, Total Direct Method Method 10129 LR (0.3 to 20.0 mg/L C) Scope and Application: For water, drinking water, and wastewater U.S. Patent 6,368,870 Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. A reagent blank is required for each series of samples.
Page 518 Organic Carbon, Total LR (0.3 to 20.0 mg/L C) ® 5. Label two Low Range 6. Use a funnel to add 7. Use a TenSette Pipet 8. Rinse two blue Low Acid Digestion vials the contents of one TOC to add 3.0 mL of Range Indicator Ampules sample and reagent blank.
Page 519 Organic Carbon, Total LR (0.3 to 20.0 mg/L C) 13. Wipe the reagent 14. Insert the reagent 15. Press 16. Wipe the sample vial ZERO blank with a damp towel, blank vial assembly in the assembly with a damp The display will show: followed by a dry one, to 16-mm round cell holder.
Page 520: Sampling And Storage
Organic Carbon, Total LR (0.3 to 20.0 mg/L C) Table 1 Interfering Substances and Levels (continued) Substance Maximum Level Tested Iron (II) 10 mg/L Iron (III) 10 mg/L Magnesium 2000 mg/L as CaCO Manganese (VII) 1 mg/L Monochloramine 14 mg/L NH Cl as Cl –...
Page 521 Organic Carbon, Total LR (0.3 to 20.0 mg/L C) 7. Add 3.0 mL of sample to each vial. Swirl to mix. Continue the test starting at step page of this procedure. 8. Analyze each sample spike as described in the procedure above, starting with the 0.1 mL sample spike.
Page 522 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 523 Organic Carbon, Total Direct Method Method 10173 MR (15 to 150 mg/L C) Scope and Application: For wastewater and industrial waters U.S. Patent 6,368,870 Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. A reagent blank is required for each series of samples.
Page 524 Organic Carbon, Total MR (15 to 150 mg/L C) Direct Method Method 10173 HRS MIN SEC HRS MIN SEC 1. Turn on the DRB 200 2. Use a graduated 3. Add 0.4 mL of Buffer 4. Place the flask on a reactor.
Page 525 Organic Carbon, Total MR (15 to 150 mg/L C) 9. Lower one unopened 10. Cap the vial 11. Carefully remove the 12. Select the test. ampule into each Acid assemblies tightly and vial assemblies from the Install the Light Shield in Digestion vial.
Page 526 Organic Carbon, Total MR (15 to 150 mg/L C) Table 1 Interfering Substances and Levels (continued) Substance Maximum Level Tested Ammonia Nitrogen 1000 mg/L as N ASTM Wastewater No effect Bromide 500 mg/L Br Bromine 25 mg/L Br Calcium 2000 mg/L as CaCO Chloride 1500 mg/L Chlorine...
Page 527 Organic Carbon, Total MR (15 to 150 mg/L C) 2. Press . Press . A summary of the standard OPTIONS>MORE STANDARD ADDITIONS additions procedure will appear. 3. Press to accept the default values for standard concentration, sample volume, and spike volumes. Press to change these values.
Page 528 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 529 Organic Carbon, Total Direct Method Method 10128 HR (100 to 700 mg/L C) Scope and Application: For wastewater and industrial waters U.S. Patent 6,368,870 Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. A reagent blank is required for each series of samples.
Page 530 Organic Carbon, Total HR (100 to 700 mg/L C) ® 5. Label two High Range 6. Use a funnel to add 7. Use a TenSette Pipet 8. Rinse two blue MR/HR Acid Digestion vials the contents of one TOC to add 0.3 mL of Indicator Ampules with sample and reagent blank.
Page 531 Organic Carbon, Total HR (100 to 700 mg/L C) 13. Wipe the reagent 14. Press 15. Wipe the reagent vial 16. Press ZERO READ blank vial assembly and assembly and insert it into The display will show: Results are in mg/L C. insert it into the 16-mm the 16-mm round cell 0 mg/L C.
Page 532 Organic Carbon, Total HR (100 to 700 mg/L C) Table 1 Interfering Substances and Levels (continued) Substance Maximum Level Tested 2– Sulfide 20 mg/L S 2– Sulfite 50 mg/L SO Zinc 5 mg/L If the sample contains greater than 1000 mg/L CaCO alkalinity, lower the sample pH to less than 7 before testing by adding Sulfuric Acid Solution*.
Page 533 Organic Carbon, Total HR (100 to 700 mg/L C) Standard Solutions Method 1. Prepare a 1000-mg/L organic carbon stock standard by dissolving 2.1254 g dry primary standard Potassium Acid Phthalate in Organic-Free Reagent Water and dilute to 1000 mL. This stock standard is stable for about 1 month at room temperature. Alternatively, open one ampule of TOC Standard Solution, 1000 mg/L C*.
Page 534 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 535: Oxygen, Dissolved
Oxygen, Dissolved HRDO Method Method 8166 ® HR (0.3 to 15.0 mg/L O AccuVac Ampuls Scope and Application: For water and wastewater Test Preparation Before starting the test: Analyze samples on-site. Do not store for later analysis Collect the following items: Quantity ®...
Page 536 Oxygen, Dissolved HR (0.3 to 15.0 mg/L O2) 5. Prepared Sample: Fill 6. Hold the Ampul with 7. Shake the Ampul for 8. Press TIMER>OK a High Range Dissolved the tip pointing down and 30 seconds. A two-minute reaction Oxygen AccuVac Ampul immediately insert the A small amount of period will begin.
Page 537: Sample Collection, Preservation, And Storage
Oxygen, Dissolved HR (0.3 to 15.0 mg/L O2) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Greater than 10 mg/L Greater than 10 mg/L Greater than 10 mg/L Magnesium is commonly present in seawater and causes a negative interference. If the sample contains more than 50% seawater, the oxygen concentration obtained by this method will be 25% less than the true oxygen concentration.
Page 538 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 539 Oxygen, Dissolved Ultra High Range Method Method 8333 ® AccuVac Ampul UHR (1.0 to 40.0 mg/L O Scope and Application: For aquaculture Test Preparation Before starting the test: Analyze samples on-site. Do not store for later analysis. Collect the following items: Quantity ®...
Page 540 Oxygen, Dissolved UHR (1.0 to 40.0 mg/L O 5. Prepared Sample: 6. Hold the Ampul with 7. Shake the Ampul for 8. Touch > TIMER Fill a High Range the tip pointing down and 30 seconds. A two-minute reaction Dissolved Oxygen immediately insert the A small amount of period will begin.
Page 541 Oxygen, Dissolved UHR (1.0 to 40.0 mg/L O Sample Collection, Preservation, and Storage The main consideration in sampling with the High Range Dissolved Oxygen AccuVac Ampul is to prevent the sample from becoming contaminated with atmospheric oxygen between breaking open the Ampul and reading the absorbance. This is accomplished by capping the Ampul with an Ampul cap.
Page 542 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 543 Oxygen, Dissolved Indigo Carmine Method Method 8316 ® AccuVac Ampuls LR (6 to 800 µg/L O Scope and Application: For boiler feedwater Test Preparation Before starting the test: The Ampuls will contain a small piece of wire to maintain reagent quality. The solution will be yellow. Collect the following items: Quantity ®...
Page 544 Oxygen, Dissolved LR (6 to 800 µg/L O HRS MIN SEC HRS MIN SEC 5. Press 6. Fill a Low Range 7. Immediately insert the 8. Use the initial reading. ZERO Dissolved Oxygen Ampul Ampul into the cell holder. The reading is stable for The display will show: with sample.
Page 545 Oxygen, Dissolved LR (6 to 800 µg/L O Summary of Method The Low Range Dissolved Oxygen AccuVac Ampul contains reagent vacuum-sealed in a 14-mL Ampul. When the AccuVac Ampul is broken open in a sample containing dissolved oxygen, the yellow solution will turn blue. The blue color development is proportional to the concentration of dissolved oxygen.
Page 546 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 547: Oxygen Demand, Chemical
Oxygen Demand, Chemical Reactor Digestion Method Method 8000 TNTplus™ LR (TNT821, 3–150 COD); HR (TNT822, 20–1500mg/L COD) Scope and Application: For water, wastewater; digestion is required; 3–150 mg/L and 20– 1500 mg/L COD ranges are USEPA approved for wastewater analyses Jirka, A.M.;...
Page 548 Oxygen Demand, Chemical LR (TNT821, 3–150 COD); HR (TNT822, 20–1500mg/L COD) TNT Plus Method 8000 Important Note: If the sample does not contain suspended solids, omit steps and 3. 1. Turn on the DRB200 2. Homogenize 100 mL 3. To help ensure that a 4.
Page 549: Sampling And Storage
Oxygen Demand, Chemical LR (TNT821, 3–150 COD); HR (TNT822, 20–1500mg/L COD) 9. Place the vial into a 10. Thoroughly clean the 11. Insert the vial into the rack to cool to room outside of the vial. cell holder. Close the lid. temperature.
Page 550 Oxygen Demand, Chemical LR (TNT821, 3–150 COD); HR (TNT822, 20–1500mg/L COD) Accuracy Check Standard Solution Method 1. Check the accuracy of the 3 to 150 mg/L range with a 100 mg/L standard. Prepare by dissolving 85 mg of dried (120 °C, overnight) potassium acid phthalate (KHP) in 1 liter of deionized water.
Page 551 Oxygen Demand, Chemical LR (TNT821, 3–150 COD); HR (TNT822, 20–1500mg/L COD) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Select the appropriate TNTplus™ COD Digestion Reagent Vial: Low Range, 3 to 150 mg/L COD 1–2 vials 25/pkg TNT821 High Range, 20 to 1500 mg/L COD 1–2 vials...
Page 552 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 553 Oxygen Demand, Chemical Reactor Digestion Method Method 8000 (0.7 to 40.0 , 3 to 150, 20 to 1500 and 200 to 15,000 mg/L COD) Scope and Application: For water, wastewater, and seawater; digestion is required; 3–150 mg/L and 20– 1500 mg/L COD ranges are USEPA approved for wastewater analyses 200–15,000 mg/L COD range are not USEPA approved.
Page 554 Oxygen Demand, Chemical (0.7 to 40.0, 3 to 150, 20 to 1500 and 200 to 15,000 mg/L COD) Digestion Procedure Method 8000 1. Homogenize 100 mL 2. For the 3. Turn on the DRB200 4. Remove the caps from of sample for 30 seconds 200–15,000 mg/L range or Reactor.
Page 555 1. Select the ultra-low 2. Clean the outside of 3. Insert the blank into 4. Press ZERO range (DR 2800 only), low the vials with a damp towel the 16-mm cell holder. The display will show: range, or high range test.
Page 556 Oxygen Demand, Chemical (0.7 to 40.0, 3 to 150, 20 to 1500 and 200 to 15,000 mg/L COD) 5. Insert the sample vial 6. Press 7. If using High Range READ into the 16-mm cell holder. Plus COD Digestion Results are in mg/L COD. Reagent Vials, multiply the result by 10.
Page 557 Important Note: For the DR 2700, begin the Standard Solution Method at step 2. 1. Check the accuracy of the 0.7 to 40.0 mg/L range (DR 2800 only) with a 30 mg/L COD standard solution. Using class A glassware, prepare a 1000 mg/L solution by diluting 850 mg dried (120 °C, overnight) potassium acid phthalate (KHP) in 1000 mL of organic–free...
Page 558 Oxygen Demand, Chemical (0.7 to 40.0, 3 to 150, 20 to 1500 and 200 to 15,000 mg/L COD) 4. Check the accuracy of the 200 to 15,000 mg/L range by using a 10,000 mg/L COD standard solution. Prepare the 10,000 mg/L solution by dissolving 8.500 g of dried (120 °C, overnight) KHP in 1 liter of deionized water.
Page 559 Oxygen Demand, Chemical (0.7 to 40.0, 3 to 150, 20 to 1500 and 200 to 15,000 mg/L COD) Alternate Reagents Description Quantity/Test Unit Cat. No. Select the appropriate COD Digestion Reagent Vial: COD2, Low Range, 0 to 150 mg/L COD 1–2 vials 25/pkg 25650-25...
Page 560 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 561 Oxygen Demand, Chemical Manganese III Reactor Digestion Method (with optional chloride removal) Method 10067 (30 to 1000 mg/L COD Mn) Scope and Application: For water and wastewater U.S. Patent 5,556,787 Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. ®...
Page 562 Oxygen Demand, Chemical (30 to 1000 mg/L COD Mn) Preparing the Acidified Sample 1. Turn on the DRB200 2. Homogenize 100 mL 3. Blank Preparation: 4. Prepared Sample: Reactor and heat to of sample for 30 seconds Pipet 9.0 mL of deionized Pipet 9.0 mL of 150 °C.
Page 563 Oxygen Demand, Chemical (30 to 1000 mg/L COD Mn) Table 1 Multiplication Factors Sample (mL) Deionized Water (mL) Range (mg/L COD) Multiplication Factor 30–1500 60–3000 180–9000 360–18,000 For best results, use 0.5 mL or more of sample for diluting. If sample values exceed 18,000 mg/L COD, use a separate sample dilution before performing the sample chloride removal procedure.
Page 564 Oxygen Demand, Chemical (30 to 1000 mg/L COD Mn) Below 5. Pipet 0.60 mL of 6. Close the vacuum 7. Open the VPD 8. Proceed to Sample acidified sample (see regulator valve completely regulator valve to release Preparation and Preparing the Acidified to achieve full vacuum.
Page 565 Oxygen Demand, Chemical (30 to 1000 mg/L COD Mn) HRS MIN SEC HRS MIN SEC 5. Insert the vials in the 6. Insert the vials in a 7. Cool the vials to room 8. Remove the vials from DRB200 Reactor at cooling rack for two temperature in a cool the water and wipe with a...
Page 566: Sample Collection, Preservation, And Storage
Oxygen Demand, Chemical (30 to 1000 mg/L COD Mn) 13. Wipe the prepared 14. Press READ sample and insert it into Results are in mg/L COD the cell holder. Interferences Inorganic materials may also be oxidized by trivalent manganese and constitute a positive interference when present in significant amounts.
Page 567 Oxygen Demand, Chemical (30 to 1000 mg/L COD Mn) Summary of Method Chemical Oxygen Demand (COD) is defined as “... a measure of the oxygen equivalent of the organic matter content of a sample that is susceptible to oxidation by a strong chemical oxidant”...
Page 568 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 569 Oxygen Demand, Chemical Manganese III Reactor Digestion Method (without chloride removal) Method 10067 (30 to 1000 mg/L COD Mn) Scope and Application: For water and wastewater U.S. Patent 5,556,787 Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. ®...
Page 570 Oxygen Demand, Chemical (30 to 1000 mg/L COD Mn) Without Chloride Removal Method 10067 1. Select the test. 2. Turn on the DRB200 3. Homogenize 100 mL 4. Pipet 0.5 mL of Reactor and heat to of sample for 30 seconds homogenized sample into Install the Light Shield in 150 °C.
Page 571 Oxygen Demand, Chemical (30 to 1000 mg/L COD Mn) 9. Invert the vials several 10. Wipe the blank and 11. Press . The 12. Wipe the sample and ZERO times to mix. insert it into the 16-mm display will show: insert it into the 16-mm round cell holder.
Page 572 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 573 Oxygen Demand, Chemical Reactor Digestion Method Method 10211 TNTplus™ 820 ULR (1–60 mg/L COD) Scope and Application: For wastewater, process water, surface water, and cooling water; digestion is required Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on package.
Page 574 Oxygen Demand, Chemical ULR (1–60 mg/L COD) TNTplus Method 10211 1. Turn on the DRB200 2. Homogenize 100 mL 3. To help ensure that a 4. Invert a vial several Reactor. Preheat to of sample for 30 seconds representative portion of times to bring the 150 °C.
Page 575: Sampling And Storage
Oxygen Demand, Chemical ULR (1–60 mg/L COD) 9. Invert the vial several 10. Place the vial into a 11. Using the Zero vial 12. Thoroughly clean the times while still hot. rack to cool to room from the sample vial lot, outside of the vial and temperature.
Page 576 Oxygen Demand, Chemical ULR (1–60 mg/L COD) Accuracy Check Standard Solution Method 1. Check the accuracy of the 1 to 60 mg/L range with a 50 mg/L standard. Prepare by dissolving 42.5 mg of dried (120 °C, overnight) potassium acid phthalate (KHP) in 1 liter of deionized water.
Page 577 Oxygen Demand, Chemical ULR (1–60 mg/L COD) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Oxygen Demand, ULR TNT820 Reagent Set 1–2 vials 24/pkg TNT820 Required Apparatus Description Quantity/Test Unit Cat. No. DRB200 Reactor, 115 V, 9x13 mm + 2x20mm (mono block) each DRB200-01 DRB200 Reactor, 230 V, 9x13 mm + 2x20mm (mono block)
Page 578 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 579 Oxygen Demand, Chemical Reactor Digestion Method Method 10212 TNTplus™ 823 UHR (250–15,000 mg/L COD) Scope and Application: For wastewater and process waters; digestion is required Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. Please read Safety Advice and Expiration Date on package.
Page 580 Oxygen Demand, Chemical UHR (250–15,000 mg/L COD) TNTplus Method 10212 1. Turn on the DRB200 2. Homogenize 100 mL 3. To help ensure that a 4. Invert a vial several Reactor. Preheat to of sample for 30 seconds representative portion of times to bring the 150 °C.
Page 581 Oxygen Demand, Chemical UHR (250–15,000 mg/L COD) 9. Invert the vial several 10. Place the vial into a 11. Thoroughly clean the 12. Insert the vial into the times while still hot. rack to cool to room outside of the vial. cell holder.
Page 582 Oxygen Demand, Chemical UHR (250–15,000 mg/L COD) Accuracy Check Standard Solution Method 1. Check the accuracy of the method range with a 1000 mg/L standard. Prepare by dissolving 0.8503 g of dried (120 °C, overnight) potassium acid phthalate (KHP) in 1 liter of deionized water.
Page 583 Oxygen Demand, Chemical UHR (250–15,000 mg/L COD) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Oxygen Demand, UHR TNT823 Reagent Set 1–2 vials 25/pkg TNT823 Required Apparatus Description Quantity/Test Unit Cat. No. DRB200 Reactor, 115 V, 9x13mm + 2x20mm (mono block) each DRB200-01 DRB200 Reactor, 230 V, 9x13mm + 2x20mm (mono block)
Page 584 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 585: Oxygen Scavengers
Oxygen Scavengers Iron Reduction Method for Oxygen Scavengers Method 8140 Powder Pillows 5 to 600 µg/L carbohydrazide; 3 to 450 µg/L DEHA; 9 to 1000 µg/L hydroquinone; 13 to 1500 µg/L iso-ascorbic acid [ISA]; 15 to 1000 µg/L methylethyl ketoxime [MEKO] Scope and Application: For testing residual corrosion inhibitors (oxygen scavengers) in boiler feed water or condensate Test Preparation Before starting the test:...
Page 586 Oxygen Scavengers Powder Pillows Method 8140 1. Press 2. Select one of the 3. Press 4. Prepared Sample: START tests. Fill a mixing bottle with STORED PROGRAMS 25 mL of sample. When determining oxygen scavengers that react quickly with oxygen at room temperature, cap the bottle.
Page 587 Oxygen Scavengers 9. When the timer 10. Immediately after 11. Immediately wipe the 12. Press READ expires, transfer the blank transferring to the 10-mL prepared sample and Results are in µg/L. and prepared samples into cell, wipe the blank and insert it into the cell holder square sample cells.
Page 588: Sample Collection, Preservation, And Storage
Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 589 Ozone Indigo Method Method 8311 ® AccuVac Ampul LR (0.01 to 0.25 mg/L O MR (0.01 to 0.75 mg/L O HR (0.01 to 1.50 mg/L O Scope and Application: For water Test Preparation Before starting the test: Analyze sample immediately. Do not preserve for later analysis. Use tap water or deionized water for the blank (ozone-free water) The sequence of measuring the blank and the sample is reversed in this procedure.
Page 590 Ozone 5. Fill one Indigo Ozone 6. Quickly invert both 7. Wipe the Ampuls with 8. Insert the blank into Reagent AccuVac Ampul Ampuls several times to a cloth to remove the cell holder. with the sample and mix. fingerprints or other Press .
Page 591 Ozone Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. ® Select one or more Ozone AccuVac Ampuls based on range: 0–0.25 mg/L 25/pkg 25160-25 0–0.75 mg/L 25/pkg 25170-25 0–1.5 mg/L 25/pkg 25180-25 Required Apparatus (AccuVac) Description Unit Cat.
Page 592 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 593: Immunoassay Method
PCB (Polychlorinated Biphenyls) Immunoassay Method Method 10050 Scope and Application: For soil This test is semi-quantitative. Results are expressed as greater or less than the threshold value used. Test Preparation This method analyzes for PCB that has been extracted from soil samples. Sample extracts, calibrators, and reagents are added to cuvettes coated with PCB-specific antibodies.
Page 594 PCB (Polychlorinated Biphenyls) Soil Extraction Procedure 1. Weigh out 5 g of soil in 2. Carefully pour the soil 3. Use the 5-gram scoop 4. Use the graduated the plastic weighing boat. into an extraction vial. to add one scoop of cylinder to transfer 10 mL sodium sulfate to the of Soil Extractant into the...
Page 595 PCB (Polychlorinated Biphenyls) Immunoassay for Soil Extracts 1. Press 2. Insert Adapter A. 3. Label an Antibody 4. Insert the cuvettes into Cuvette for each calibrator the rack snugly. SINGLE WAVELENGTH and each sample to be Press and press OPTIONS tested.
Page 596 PCB (Polychlorinated Biphenyls) HRS MIN SEC HRS MIN SEC 9. After 5 minutes mix 10. At the end of the 11. Wash each cuvette the contents of the rack for 10-minute period, discard forcefully and thoroughly 30 seconds (Using the the contents of all the four times with deionized 1-cm MicroCuvette Rack...
Page 597 PCB (Polychlorinated Biphenyls) Measuring the Color 16. Label and fill a Zeroing 17. Insert the filled zeroing 18. Press 19. Insert the first ZERO Cuvette with deionized cuvette into the cell holder calibrator into the cell The display will show: water.
Page 598 PCB (Polychlorinated Biphenyls) ® Using the Wiretrol * Pipet The Wiretrol Pipet can accurately measure small quantities of liquids. It consists of two parts: ® a Teflon -tipped plunger and a calibrated capillary tube. The plunger can be reused; the capillary tubes must be discarded after one use.
Page 599 PCB (Polychlorinated Biphenyls) Loading the Rack—The cuvette rack is designed so that it may be inverted with the cuvettes in place. Identify each cuvette with a sample or calibrator number and insert all the cuvettes in the rack before beginning the procedure. Fit the cuvettes snugly into the rack, but do not force them or they may be difficult to remove and their contents may spill.
Page 600: Sample Collection And Storage
PCB (Polychlorinated Biphenyls) Sensitivity The PCB immunoassay cannot differentiate between the various Aroclors, but it detects their presence in differing degrees. Table 3 Various PCBs in Soil Concentration (ppm) to give a positive result at Compound 1 ppm 5 ppm 10 ppm 50 ppm 1248...
Page 601 PCB (Polychlorinated Biphenyls) Consumables and Replacement Items Required Reagents Description Unit Cat. No. PCB Reagent Set 20 cuvettes 27735-00 Deionized Water 500 mL 242-49 Immunoassay components are manufactured by Beacon Analytical Systems, Inc. Required Apparatus Description Unit Cat. No. Adapter, 1-cm square cell each Caps, flip spout 2/pkg...
Page 602 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 603 Phenols 4-Aminoantipyrine Method Method 8047 (0.002 to 0.200 mg/L) Scope and Application: For water and wastewater; USEPA accepted (distillation required) Adapted from Standard Methods for the Examination of Water and Wastewater Procedure is equivalent to USEPA method 420.1 for wastewater Test Preparation Before starting the test: Analyze samples within four hours to avoid oxidation.
Page 604: Stored Programs
Phenols (0.002 to 0.200 mg/L) 4-Aminoantipyrine Method 8047 1. Press 2. Select the test. 3. Measure 300 mL of 4. Blank Preparation: deionized water in a Pour the measured STORED PROGRAMS 500-mL graduated deionized water into a cylinder. 500-mL separatory funnel. 5.
Page 605: Accuracy Check
Phenols (0.002 to 0.200 mg/L) 13. Insert a large, 14. Drain the chloroform 15. Wipe the blank and 16. Wipe the prepared pea-sized cotton plug into layers into separate insert it into the cell holder sample and insert it into the delivery tube of each sample cells (one for the with the fill line facing right.
Page 606 Phenols (0.002 to 0.200 mg/L) 2. Pipet 10.0 mL of the 1000-mg/L stock solution to a 1000-mL volumetric flask. Dilute to the mark with deionized water. This is a 10-mg/L working solution. 3. Prepare a 0.200-mg/L standard solution by pipetting 10.0 mL of the working solution into a 500-mL volumetric flask.
Page 607 Phenols (0.002 to 0.200 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Phenols Reagent Set (100 Tests), includes: — — 22439-00 (2) Chloroform, ACS 60 mL 14458-17 (3) Hardness 1 Buffer Solution, pH 10.1 10 mL 500 mL 424-49 (2) Phenol 2 Reagent Powder Pillows...
Page 608 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 609 Phosphonates Persulfate UV Oxidation Method Method 8007 Powder Pillows (0.02 to 2.50 and 1.0 to 125.0 mg/L) Scope and Application: For boiler and cooling water, wastewater, and seawater Adapted from Blystone, P., Larson, P., A Rapid Method for Analysis of Phosphate Compounds, International Water Conference, Pittsburgh, PA. (Oct 26-28, 1981) Test Preparation Before starting the test:...
Page 610: Powder Pillows
Phosphonates (0.02 to 2.50 and 1.0 to 125.0 mg/L) Powder Pillows Method 8007 1. Press 2. Select the test. 3. Choose the 4. Blank Preparation: appropriate sample size Fill a square sample cell to STORED PROGRAMS from Table 1 on page the 10-mL mark with Pipet the chosen volume diluted sample from...
Page 611 Phosphonates (0.02 to 2.50 and 1.0 to 125.0 mg/L) 9. When the timer 10. Prepared Sample: 11. Add the contents of 12. Press TIMER>OK expires, turn off the UV Fill a second square one PhosVer 3 Phosphate A two-minute reaction lamp and remove it from sample cell to the 10-mL Reagent Powder Pillow to...
Page 612 Phosphonates (0.02 to 2.50 and 1.0 to 125.0 mg/L) Table 1 Expected Ranges with Multipliers Expected Range Sample Volume (mL) Multiplier (mg/L phosphonate) 0–2.5 0–5 0–12.5 0–25 0–125 To express results in terms of active phosphonate, multiply the final value in step by the appropriate conversion factor in Table...
Page 613 Phosphonates (0.02 to 2.50 and 1.0 to 125.0 mg/L) Table 3 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Silica 500 mg/L Silicate 100 mg/L Sulfate 2000 mg/L Sulfide Interferes at all levels Sulfite 100 mg/L Thiourea 10 mg/L Highly buffered samples or extreme May exceed the buffering capacity of the reagents and require sample pretreatment.
Page 614 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 615 Phosphorus, Acid Hydrolyzable Digestion Acid Digestion Method Method 8180 Scope and Application: For water, wastewater, and seawater; USEPA Accepted for wastewater analyses Adapted from Standard Methods for the Examination of Water and Wastewater 4500-P B & E Test Preparation Before starting the test: Rinse all glassware with 1:1 hydrochloric acid.
Page 616 Phosphorus, Acid Hydrolyzable Digestion Acid Digestion Method 8180 HRS MIN SEC HRS MIN SEC 1. Use a graduated 2. Use a 1-mL calibrated 3. Place the flask on a 4. Cool the sample to cylinder to measure 25 mL dropper to add 2.0 mL of hot plate.
Page 617 Phosphorus, Acid Hydrolyzable Digestion Interferences Table 1 Interfering Substances and Levels Interfering Interference Levels and Treatments Substance Alkaline or highly buffered It may be necessary to add additional acid in step to drop the pH of the solution below 1. samples Use 50 mL of sample and double the reagent quantities.
Page 618 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 619: Phosphorus, Acid Hydrolyzable
Phosphorus, Acid Hydrolyzable PhosVer™ 3 with Acid Hydrolysis Method Method 8180 3– Test ‘N Tube™ Vials (0.06 to 3.50 mg/L PO Scope and Application: For water, wastewater, and seawater Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test. For more accurate results, determine a reagent blank value for each new lot of reagent.
Page 620 3– Phosphorus, Acid Hydrolyzable (0.06 to 3.50 mg/L PO Test ‘N Tube Method 8180 1. Turn on the DRB200 2. Select the test. 3. Use a TenSette Pipet 4. Insert the vial into the Reactor. Preheat to to add 5 mL of sample to a preheated DRB200 Install the Light Shield in 150 °C.
Page 621 3– Phosphorus, Acid Hydrolyzable (0.06 to 3.50 mg/L PO 9. Insert the sample vial 10. Press 11. Using a funnel, add 12. Immediately cap ZERO into the 16-mm round cell the contents of one tightly and shake to mix for The display will show: holder.
Page 622 3– Phosphorus, Acid Hydrolyzable (0.06 to 3.50 mg/L PO Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Aluminum Greater than 200 mg/L Arsenate All levels Chromium Greater than 100 mg/L Copper Greater than 10 mg/L Iron Greater than 100 mg/L Nickel...
Page 623 3– Phosphorus, Acid Hydrolyzable (0.06 to 3.50 mg/L PO 5. Prepare three sample spikes. Fill three Mixing Cylinders with 25 mL of sample. Use the ® TenSette Pipet to add 0.1 mL, 0.2 mL, and 0.3 mL of standard, respectively, to each sample and mix thoroughly.
Page 624 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 625: Phosphorus, Total, Digestion
Phosphorus, Total, Digestion Acid Persulfate Digestion Method Method 8190 Scope and Application: For water, wastewater, and seawater; USEPA Accepted for wastewater analyses when used with the ascorbic acid (PhosVer 3) method. Adapted from Standard Methods for the Examination of Water and Wastewater 4500-P B & E Test Preparation Before starting the test: Rinse all glassware with 1:1 hydrochloric acid.
Page 626 Phosphorus, Total, Digestion Acid Digestion Method 8190 HRS MIN SEC HRS MIN SEC 1. Use a graduated 2. Add the contents of 3. Use a 1-mL calibrated 4. Place the flask on a cylinder to measure 25 mL one Potassium Persulfate dropper to add 2.0 mL of hot plate.
Page 627 Phosphorus, Total, Digestion Interferences Table 1 Interfering Substances and Levels Interfering Interference Levels and Treatments Substance Alkaline or highly It may be necessary to add additional acid in step to drop the pH of the solution below 1. buffered samples Use 50 mL of sample and double the reagent quantities.
Page 628 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 629: Phosphorus, Reactive (Orthophosphate)
Phosphorus, Reactive (Orthophosphate) Amino Acid Method Method 8178 3– (0.23 to 30.00 mg/L PO Scope and Application: For water, wastewater, and seawater Adapted from Standard Methods for the Examination of Water and Wastewater Test Preparation Before starting the test: For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water in place of the sample.
Page 630 3– Phosphorus, Reactive (Orthophosphate) (0.23 to 30.00 mg/L PO 6. Press 7. Blank Preparation: 8. When the timer Prepared Sample: TIMER>OK 1 mL of Amino Acid Fill a square sample cell expires, wipe the blank A 10-minute reaction Reagent Solution. Stopper with untreated sample.
Page 631 3– Phosphorus, Reactive (Orthophosphate) (0.23 to 30.00 mg/L PO Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Sulfide interferes. For samples with sulfide concentration less than 5 mg/L sulfide interference may be removed by oxidation with Bromine Water as follows: Measure 50 mL of sample into an Erlenmeyer flask.
Page 632 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 633: Phosphorus, Reactive
Phosphorus, Reactive Molybdovanadate Rapid Liquid Method Method 8114 3– Pour-Thru Cell HR (0.3 to 45.0 mg/L PO Scope and Application: For treated and natural waters Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: See the User Manual for Pour-Thru Module installation instructions.
Page 634: Phosphorus, Reactive Hr
3– Phosphorus, Reactive HR (0.3 to 45.0 mg/L PO 5. Rinse another clean 6. Prepared Sample: 7. Add 1.0 mL of 8. Press TIMER>OK plastic 125-mL Erlenmeyer Measure 25 mL of sample Molybdovanadate A 7-minute reaction period flask with deionized water. in the graduated cylinder.
Page 635: Sampling And Storage
3– Phosphorus, Reactive HR (0.3 to 45.0 mg/L PO Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Arsenate Negative interference. Positive interference if sample is heated. Bismuth Negative interference. Fluoride Negative interference. Blue color is caused by ferrous iron but this does not affect results if the ferrous iron Iron, Ferrous concentration is less than 100 mg/L.
Page 636 3– Phosphorus, Reactive HR (0.3 to 45.0 mg/L PO Collect samples in clean plastic or glass bottles that have been cleaned with 1:1 Hydrochloric Acid Solution* and rinsed with deionized water. Do not use detergents that contain phosphate for cleaning labware. Analyze samples immediately for best results.
Page 637 3– Phosphorus, Reactive HR (0.3 to 45.0 mg/L PO Standard Solution Method 1. Use a 10.0-mg/L Phosphate Standard in place of the sample. 3– 2. To adjust the calibration curve using the reading obtained with the 10-mg/L PO Phosphate Standard Solution, press on the current program menu.
Page 638 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 639 Phosphorus, Reactive (Orthophosphate) Molybdovanadate Method Method 8114 ® 3– Reagent Solution or AccuVac Ampuls (0.3 to 45.0 mg/L PO Scope and Application: For water and wastewater Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: For best results, sample temperature should be 20–25 °C (68–77 °F).
Page 640 3– Phosphorus, Reactive (Orthophosphate) (0.3 to 45.0 mg/L PO 5. Add 0.5 mL of 6. Press 7. When the timer 8. Wipe the prepared TIMER>OK Molybdovanadate expires, wipe the blank sample and insert it into A 7-minute reaction period Reagent to each sample and insert it into the cell the cell holder with the fill will begin.
Page 641 3– Phosphorus, Reactive (Orthophosphate) (0.3 to 45.0 mg/L PO 5. Press 6. When the timer 7. Press 8. Wipe the prepared TIMER>OK ZERO expires, wipe the blank sample and insert it into A 7-minute reaction period The display will show: and insert it into the cell the cell holder.
Page 642: Standard Additions
3– Phosphorus, Reactive (Orthophosphate) (0.3 to 45.0 mg/L PO Table 2 shows substances that do not interfere in concentrations less than 1000 mg/L. Table 2 Noninterfering at Low Concentrations Pyrophosphate Tetraborate Benzoate Citrate Lactate Formate Oxalate Tartrate Salicylate – – 2–...
Page 643 3– Phosphorus, Reactive (Orthophosphate) (0.3 to 45.0 mg/L PO 6. Analyze each sample spike as described in the procedure above, starting with the 0.1 mL sample spike. Accept each standard additions reading by pressing . Each addition READ should reflect approximately 100% recovery. 7.
Page 644 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 645 Phosphorus, Reactive (Orthophosphate) Molybdovanadate Method Method 8114 3– Test ‘N Tube™ Vials HR (1.0 to 100.0 mg/L PO Scope and Application: For water and wastewater. Adapted from Standard Methods for the Examination of Water and Wastewater Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 646 3– Phosphorus, Reactive (Orthophosphate) HR (1.0 to 100.0 mg/L PO Test ‘N Tube Method 8114 1. Select the test. 2. Blank Preparation: 3. Prepared Sample: 4. Press TIMER>OK ® Use a TenSette Pipet to Use a TenSette Pipet to Install the Light Shield in A seven-minute reaction add 5.0 mL of deionized add 5.0 mL of sample to a...
Page 647 3– Phosphorus, Reactive (Orthophosphate) HR (1.0 to 100.0 mg/L PO Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Extreme pH or highly May exceed buffering capacity of the reagents. Samples may require pretreatment. Sample pH buffered samples should be about 7.
Page 648 3– Phosphorus, Reactive (Orthophosphate) HR (1.0 to 100.0 mg/L PO Accuracy Check Standard Additions Method (Sample Spike) 1. Clean glassware with 1:1 hydrochloric acid solution. Rinse again with deionized water. Do not use detergents containing phosphate to clean glassware. 2. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify the chemical form.
Page 649 3– Phosphorus, Reactive (Orthophosphate) HR (1.0 to 100.0 mg/L PO Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. High Range Reactive Phosphorus Test ’N Tube™ Reagent Set, includes: — 50 vials 27673-45 (1) Reactive High Range Phosphorus Test ’N Tube Vials 50/pkg —...
Page 650 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 651 Phosphorus, Reactive Ascorbic Acid Rapid Liquid Method Method 10055 3– Pour-Thru Cell LR (19 to 3000 µg/L PO Scope and Application: For treated and natural waters Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: See the user manual for Pour-Thru Module installation instructions.
Page 652 3– Phosphorus, Reactive LR (19 to 3000 µg/L PO Pour-Thru Cell Method 10055 5. Fill the rinsed cylinder 6. Pour the contents of 7. Measure a second 8. Add 1.0 mL of to the 25-mL mark with the 25-mL cylinder into 25-mL portion of sample Molybdate reagent to each sample.
Page 653: Reagent Preparation
3– Phosphorus, Reactive LR (19 to 3000 µg/L PO Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Aluminum 200 mg/L Arsenate Interferes Chromium 100 mg/L Copper 10 mg/L Hydrogen sulfide Interferes Iron 100 mg/L Nickel 300 mg/L Silica 50 mg/L...
Page 654: Sampling And Storage
3– Phosphorus, Reactive LR (19 to 3000 µg/L PO Sampling and Storage Collect samples in clean plastic or glass bottles that have been cleaned with 1:1 Hydrochloric Acid Solution* and rinsed with deionized water. Do not use detergents that contain phosphate for cleaning labware.
Page 655 3– Phosphorus, Reactive LR (19 to 3000 µg/L PO Summary of Method Orthophosphate reacts with molybdate in an acid medium to produce a phosphomolybdate complex. Ascorbic acid then reduces the complex, giving an intense molybdenum blue color. Reactive phosphorus includes existing orthophosphate in the sample plus a small fraction of condensed phosphate that may be hydrolyzed to orthophosphate during the test.
Page 656 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 657: Powder Pillows
Phosphorus, Reactive (Orthophosphate) PhosVer 3 (Ascorbic Acid) Method Method 8048 ® 3– Powder Pillows or AccuVac Ampuls (0.02 to 2.50 mg/L PO Scope and Application: For water, wastewater, and seawater; USEPA Accepted for reporting for wastewater analyses Adapted from Standard Methods for the Examination of Water and Wastewater Procedure is equivalent to USEPA method 365.2 and Standard Method 4500-P-E for wastewater.
Page 658 3– Phosphorus, Reactive (Orthophosphate) (0.02 to 2.50 mg/L PO 5. Press 6. Blank Preparation: 7. When the timer 8. Wipe the prepared TIMER>OK Fill a second square expires, wipe the blank sample and insert it into A two-minute reaction sample cell with 10 mL of and insert it into the cell the cell holder with the fill period will begin.
Page 659 3– Phosphorus, Reactive (Orthophosphate) (0.02 to 2.50 mg/L PO HRS MIN SEC HRS MIN SEC 5. Secure an Ampul cap 6. Press 7. When the timer 8. Wipe the prepared TIMER>OK over the tip of the Ampul. expires, wipe the blank sample and insert it into A two-minute reaction Shake the Ampul for...
Page 660 3– Phosphorus, Reactive (Orthophosphate) (0.02 to 2.50 mg/L PO Sample Collection, Storage, and Preservation Collect sample in plastic or glass bottles that have been cleaned with 1:1 Hydrochloric Acid Solution* and rinsed with deionized water. Do not use commercial detergents containing phosphate for cleaning glassware used in phosphate analysis.
Page 661 3– Phosphorus, Reactive (Orthophosphate) (0.02 to 2.50 mg/L PO Standard Solution Method 1. Prepare a 2.00 mg/L phosphate standard by pipetting 4.00 mL of 50 mg/L Phosphate Standard Solution into a 100 mL volumetric flask. Dilute to volume with demineralized water and mix.
Page 662 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 663: Tips And Techniques
Phosphorus, Reactive (Orthophosphate) ® PhosVer 3 Method Method 8048 3– Test ‘N Tube™ Vials (0.06 to 5.00 mg/L PO or 0.02 to 1.60 mg/L P) Scope and Application: For water, wastewater, and seawater; USEPA accepted for reporting wastewater analysis Procedure is equivalent to USEPA Method 365.2 and Standard Method 4500-P E for wastewater. Tips and Techniques Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 664 3– Phosphorus, Reactive (Orthophosphate) (0.06 to 5.00 mg/L PO or 0.02 to 1.60 mg/L P) 5. Press Zero. 6. Using a funnel, add 7. Immediately cap the 8. Press TIMER>OK the contents of one vial tightly and shake for at The display will show: A two-minute reaction PhosVer 3 Phosphate...
Page 665 3– Phosphorus, Reactive (Orthophosphate) (0.06 to 5.00 mg/L PO or 0.02 to 1.60 mg/L P) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments (continued) Large amounts may cause inconsistent results in the test because the acid present in the Turbidity powder pillows may dissolve some of the suspended particles and because of variable desorption of orthophosphate from the particles.
Page 666 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 667 Phosphorus, Reactive (Orthophosphate) and Total Method 10209 Reactive; Method 10210 Total Ascorbic Acid Method TNTplus 843 3– LR (0.15–4.50 mg/L PO or 0.05–1.50 mg/L PO –P) Scope and Application: For wastewater, drinking water, boiler water, surface water, and process water Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 668 3– Phosphorus, Reactive (Orthophosphate) and Total LR (0.15–4.50 mg/L PO or 0.05–1.50 mg/L PO –P) TNTplus—Phosphorus, Total Method 10210 1. Turn on the DRB200 2. Carefully remove the 3. Carefully pipet 2.0 mL 4. Flip the DosiCap Zip Reactor. Heat to 100 °C. protective foil lid from the of sample into the vial.
Page 669 3– Phosphorus, Reactive (Orthophosphate) and Total LR (0.15–4.50 mg/L PO or 0.05–1.50 mg/L PO –P) 13. Clean the outside of the vial and insert it into the cell holder. The instrument reads the barcode, then selects and performs the correct test. Results are in mg/L PO No instrument Zero is required.
Page 670 3– Phosphorus, Reactive (Orthophosphate) and Total LR (0.15–4.50 mg/L PO or 0.05–1.50 mg/L PO –P) HRS MIN SEC HRS MIN SEC 5. Wait 10 minutes. 6. When the timer 7. Clean the outside of expires, invert the vial the vial and insert it into Install the Light Shield in again 2–3 times.
Page 671: Sampling And Storage
3– Phosphorus, Reactive (Orthophosphate) and Total LR (0.15–4.50 mg/L PO or 0.05–1.50 mg/L PO –P) Table 1 Interfering Substances and Levels Interfering Substance Interference Level 2– 5000 mg/L – 2000 mg/L , Na 1000 mg/L – 500 mg/L 250 mg/L 100 mg/L 2–...
Page 672 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 673 Phosphorus, Reactive (Orthophosphate) and Total Method 10209 Reactive; Method 10210 Total Ascorbic Acid Method TNTplus™ 844 3– HR (1.5 to 15.0 mg/L PO or 0.5 to 5.0 mg/L PO –P) Scope and Application: For wastewater, drinking water, boiler water, surface water, and process analysis Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 674 3– Phosphorus, Reactive (Orthophosphate) and Total HR (1.5 to 15.0 mg/L PO or 0.5 to 5.0 mg/L PO –P) TNTplus—Phosphorus, Total Method 10210 1. Turn on the DRB200 2. Carefully remove the 3. Carefully pipet 0.5 mL 4. Flip the DosiCap Zip Reactor Heat to 100 °C.
Page 675 3– Phosphorus, Reactive (Orthophosphate) and Total HR (1.5 to 15.0 mg/L PO or 0.5 to 5.0 mg/L PO –P) 13. Clean the outside of the vial and insert it into the cell holder. The instrument reads the barcode, then selects and performs the correct test.
Page 676 3– Phosphorus, Reactive (Orthophosphate) and Total HR (1.5 to 15.0 mg/L PO or 0.5 to 5.0 mg/L PO –P) HRS MIN SEC HRS MIN SEC 5. Wait 10 minutes. 6. When the timer 7. Clean the outside of expires, invert the vial the vial and insert it into Install the Light Shield in again 2–3 times.
Page 677 3– Phosphorus, Reactive (Orthophosphate) and Total HR (1.5 to 15.0 mg/L PO or 0.5 to 5.0 mg/L PO –P) Interferences The ions listed in Table 1 have been individually checked up to the given concentrations and do not cause interference. Cumulative effects and the influence of other ions have not been determined.
Page 678 3– Phosphorus, Reactive (Orthophosphate) and Total HR (1.5 to 15.0 mg/L PO or 0.5 to 5.0 mg/L PO –P) Summary of Method Phosphates present in organic and condensed inorganic forms (meta-, pyro-, or other polyphosphates) are first converted to reactive orthophosphate in the total phosphorus procedure.
Page 679 3– Phosphorus, Reactive (Orthophosphate) and Total HR (1.5 to 15.0 mg/L PO or 0.5 to 5.0 mg/L PO –P) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Phosphorus, Reactive and Total, HR TNT844 Reagent Set 25/pkg TNT844 Required Apparatus Description Quantity/Test...
Page 680 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 681 Phosphorus, Reactive (Orthophosphate) and Total Method 10209 Reactive; Method 10210 Total Ascorbic Acid Method TNTplus™ 845 3– UHR (6 to 60 mg/L PO or 2 to 20 mg/L PO –P) Scope and Application: For wastewater, drinking water, boiler water, surface water, and process analysis Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 682 3– Phosphorus, Reactive (Orthophosphate) and Total UHR (6 to 60 mg/L PO or 2 to 20 mg/L PO –P) TNTplus—Phosphorus, Total Method 10210 1. Turn on the DRB200 2. Carefully remove the 3. Carefully pipet 0.4 mL 4. Flip the DosiCap Zip Reactor Heat to 100 °C.
Page 683 3– Phosphorus, Reactive (Orthophosphate) and Total UHR (6 to 60 mg/L PO or 2 to 20 mg/L PO –P) 13. Clean the outside of the vial and insert it into the cell holder. The instrument reads the barcode, then selects and performs the correct test.
Page 684 3– Phosphorus, Reactive (Orthophosphate) and Total UHR (6 to 60 mg/L PO or 2 to 20 mg/L PO –P) HRS MIN SEC HRS MIN SEC 5. Wait 10 minutes. 6. When the timer 7. Clean the outside of expires, invert the vial the vial and insert it into Install the Light Shield in again 2–3 times.
Page 685 3– Phosphorus, Reactive (Orthophosphate) and Total UHR (6 to 60 mg/L PO or 2 to 20 mg/L PO –P) Interferences The ions listed in Table 1 have been individually checked up to the given concentrations and do not cause interference. Cumulative effects and the influence of other ions have not been determined.
Page 686 3– Phosphorus, Reactive (Orthophosphate) and Total UHR (6 to 60 mg/L PO or 2 to 20 mg/L PO –P) Summary of Method Phosphates present in organic and condensed inorganic forms (meta-, pyro-, or other polyphosphates) are first converted to reactive orthophosphate in the total phosphorus procedure.
Page 687 3– Phosphorus, Reactive (Orthophosphate) and Total UHR (6 to 60 mg/L PO or 2 to 20 mg/L PO –P) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Phosphorus, Reactive and Total, UHR TNT845 Reagent Set 25/pkg TNT845 Required Apparatus Description Quantity/Test...
Page 688 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 689: Phosphorus, Reactive (Orthophosphate)
Phosphorus, Reactive (Orthophosphate) Molybdovanadate Method Method 10214 TNTplus™ 846 3– (5.0 to 90.0 mg/L PO or 1.6 to 30 mg/L PO –P) Scope and Application: For wastewater, drinking water, boiler water, surface water, and process water Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 690 3– Phosphorus, Reactive (Orthophosphate) (5.0 to 90.0 mg/L PO or 1.6 to 30 mg/L PO –P) 5. Clean the outside of the vial and insert it into the cell holder. The instrument reads the barcode, then selects and performs the correct test. 3–...
Page 691 3– Phosphorus, Reactive (Orthophosphate) (5.0 to 90.0 mg/L PO or 1.6 to 30 mg/L PO –P) Interferences The items listed in Table 1 have been individually checked up to the given concentrations and do not cause interference. The cumulative effects and influence of other ionshas not been detemined.
Page 692 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 693: Phosphorus, Total
Phosphorus, Total ® PhosVer 3 with Acid Persulfate Digestion Method Method 8190 3– Test ‘N Tube™ Vials (0.06 to 3.50 mg/L PO or 0.02 to 1.10 mg/L P) Scope and Application: For water, wastewater, and seawater; USEPA Accepted for reporting wastewater analyses Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 694 3– Phosphorus, Total (0.06 to 3.50 mg/L PO or 0.02 to 1.10 mg/L P) Test ‘N Tube Method 8190 ® 1. Turn on the DRB200 2. Select the test. 3. Use a TenSette Pipet 4. Use a funnel to add Reactor.
Page 695 3– Phosphorus, Total (0.06 to 3.50 mg/L PO or 0.02 to 1.10 mg/L P) 9. Use a TenSette Pipet 10. Wipe the outside of 11. Insert the vial into the 12. Press ZERO to add 2 mL of 1.54 N the vial with a damp cloth 16 mm cell holder.
Page 696: Standard Additions
3– Phosphorus, Total (0.06 to 3.50 mg/L PO or 0.02 to 1.10 mg/L P) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Aluminum Greater than 200 mg/L Arsenate Interferes at any level Chromium Greater than 100 mg/L Copper Greater than 10 mg/L Iron...
Page 697 3– Phosphorus, Total (0.06 to 3.50 mg/L PO or 0.02 to 1.10 mg/L P) 6. Prepare three sample spikes. Fill three Mixing Cylinders* with 25 mL of sample. Use the ® TenSette Pipet to add 0.1 mL, 0.2 mL, and 0.3 mL of standard, respectively, to each sample and mix thoroughly.
Page 698 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 699 Phosphorus, Total Molybdovanadate Method with Acid Persulfate Digestion Method 10127 3– Test ‘N Tube™ Vials HR (1.0 to 100.0 mg/L PO Scope and Application: For water and wastewater Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 700 3– Phosphorus, Total HR (1.0 to 100.0 mg/L PO 5. Use a funnel to add 6. Insert the vials in the 7. Press 8. After the timer expires, TIMER>OK the contents of one DRB 200 Reactor. carefully remove the hot A 30-minute heating period Potassium Persulfate vials from the reactor.
Page 701 3– Phosphorus, Total HR (1.0 to 100.0 mg/L PO Interferences Large amounts of sample turbidity may cause inconsistent results in the test because the acid present in the reagents may dissolve some of the suspended particles and because of variable desorption of orthophosphate from the particles.
Page 702 3– Phosphorus, Total HR (1.0 to 100.0 mg/L PO Sampling and Storage Collect samples in plastic or glass bottles that have been acid washed with 1:1 Hydrochloric Acid Solution* and rinsed with deionized water. Do not use commercial detergents containing phosphate for cleaning the glassware used in this test.
Page 703 3– Phosphorus, Total HR (1.0 to 100.0 mg/L PO Summary of Method Phosphates present in organic and condensed inorganic forms (meta-, pyro-, or other polyphosphates) must be converted to reactive orthophosphate before analysis. Pretreatment of the sample with acid and heat provides the conditions for hydrolysis of the condensed inorganic forms.
Page 704 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 705 Potassium Tetraphenylborate Method Method 8049 Powder Pillows (0.1 to 7.0 mg/L) Scope and Application: For water, wastewater, and seawater Test Preparation Before starting the test: Program # 905 has a calibration curve for potassium; however, due to potential variation between lots of Potassium 3 Reagent, perform a new calibration for each lot of reagent to obtain best accuracy.
Page 706: Powder Pillows
Potassium (0.1 to 7.0 mg/L) Powder Pillows Method 8049 1. Press 2. Select the test. 3. Fill a graduated mixing 4. Add the contents of cylinder with 25 mL of one Potassium 1 Reagent USER PROGRAMS When performing this sample. Pillow.
Page 707: Sample Collection, Preservation, And Storage
Potassium (0.1 to 7.0 mg/L) 9. Wipe the blank and 10. Press 11. Within seven minutes 12. Press ZERO READ insert it into the cell holder after the timer expires, The display will show: Results are in mg/L K. with the fill line facing right. wipe the prepared sample 0.0 mg/L K and insert it into the cell...
Page 708 Potassium (0.1 to 7.0 mg/L) 3. Press to accept the default values for standard concentration, sample volume, and spike volumes. Press to change these values. After values are accepted, the EDIT unspiked sample reading will appear in the top row. See the user manual for more information.
Page 709 Potassium (0.1 to 7.0 mg/L) 6. To enter the remainder of the test parameters, press each line to highlight it, press EDIT then enter the value specified below. Press to accept the value, and press again to return to the list. Set up the following parameters as: •...
Page 710 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 711: Quaternary Ammonium Compounds
Quaternary Ammonium Compounds Direct Binary Complex Method Method 8337 Powder Pillows (0.2 to 5.0 mg/L as CTAB) Scope and Application: For cooling tower water and pool/spa water Test Preparation Collect the following items: Quantity QAC Reagent 1 Powder Pillows 2 pillows QAC Reagent 2 Powder Pillows 2 pillows Bottle, square, with 25 mL mark...
Page 712 Quaternary Ammonium Compounds (0.2 to 5.0 mg/L as CTAB) 5. Add the contents of 6. Swirl the bottles to 7. Add the contents of 8. Swirl the bottles to one QAC Reagent 1 dissolve the reagent. one QAC Reagent 2 dissolve the reagent.
Page 713 Quaternary Ammonium Compounds (0.2 to 5.0 mg/L as CTAB) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments Magnesium, Mg Positive interference above 1350 mg/L Niaproof™ anionic surfactant Negative interference above 11 mg/L Polyacrylic acid Negative interference above 16 mg/L Sodium lauryl sulfate Negative interference above 8 mg/L Sodium polyphosphate...
Page 714 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 715 Selenium Diaminobenzidine Method Method 8194 (0.01 to 1.00 mg/L) Scope and Application: For water and wastewater Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: Distillation is required for determining total selenium. See Distillation on page 5 at the end of the procedure.
Page 716: Stored Programs
Selenium (0.01 to 1.00 mg/L) Diaminobenzidine Method 8194 1. Press 2. Select the test. 3. Measure 100 mL of 4. Add a 0.2-g spoonful ® deionized water into a of TitraVer Hardness STORED PROGRAMS 500-mL Erlenmeyer flask. Reagent to each flask. Label the flask “blank”.
Page 717 Selenium (0.01 to 1.00 mg/L) 9. When the timer 10. Transfer the contents 11. Add 2.0 mL of 12 N 12. Add 30-mL of toluene expires, remove both of each flask to separate Potassium Hydroxide to each funnel. Stopper. flasks. Cool to room 250-mL separatory Standard Solution to each Swirl and invert each...
Page 718 Selenium (0.01 to 1.00 mg/L) 17. Insert a cotton plug 18. Wipe the blank and 19. Press 20. Wipe the prepared ZERO into the delivery tube of insert it into the cell holder sample and insert it into The display will show: each separatory funnel.
Page 719 Selenium (0.01 to 1.00 mg/L) Distillation CAUTION Always perform this procedure under a fume hood! This distillation involves the use of a strong acid and oxidizer at high temperatures. To avoid personal injury, observe all laboratory safety precautions when operating the distilling apparatus.
Page 720 Selenium (0.01 to 1.00 mg/L) 17. Turn off the heater switch. When the J-tube and condenser have cooled, rinse them with deionized water. Add the washings to the 250-mL beaker. Total volume in the beaker should be approximately 100 mL. 18.
Page 721 Selenium (0.01 to 1.00 mg/L) Standard Solution Method Prepare a 0.5-mg/L Se standard solution as follows: 1. Prepare a 100 mg/L selenium standard solution by pipetting 10 mL of 1000 mg/L Selenium Standard Solution into a 100 mL volumetric flask, and diluting to volume with demineralized water.
Page 722 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 723 Silica Heteropoly Blue Method Method 8282 Pour-Thru Cell ULR(3 to 1000 µg/L as SiO Scope and Application: For testing trace levels of soluble silica in pure and ultrapure water Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: See the user manual for Pour-Thru Module installation instructions.
Page 724 Silica ULR(3 to 1000 µg/L as SiO Pour-Thru Cell Method 8282 1. Select the test. 2. Account for the 3. To adjust the reagent 4. Insert Adapter B. Molybdate 3 reagent blank blank value, press the Install the Pour-Thru Cell by pressing existing value and adjust it with the 1-inch (round)
Page 725 Silica ULR(3 to 1000 µg/L as SiO 13. Press 14. When the timer 15. After the flow stops, 16. Add 1.0 mL of Amino TIMER>OK expires, pour the contents press Acid F Reagent to the ZERO A one-minute reaction of one flask into the remaining flask.
Page 726: Reagent Preparation
Silica ULR(3 to 1000 µg/L as SiO Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Color Eliminated by zeroing the instrument with the original sample (follow procedure). Iron Interferes at high levels. pH (extreme) Adjust pH to less than 7. 3–...
Page 727 Silica ULR(3 to 1000 µg/L as SiO Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify the chemical form. 2. Press . Press . A summary of the standard OPTIONS>MORE STANDARD ADDITIONS additions procedure will appear.
Page 728 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 729 Silica Heteropoly Blue Rapid Liquid Method Method 8282 Pour-Thru Cell ULR(3 to 1000 µg/L as SiO Scope and Application: For testing trace levels of soluble silica in pure and ultrapure water Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: See the User Manual for Pour-Thru Module installation instructions.
Page 730 Silica ULR(3 to 1000 µg/L as SiO Pour-Thru Cell Method 8282 1. Select the test. 2. Account for the 3. To adjust the reagent 4. Insert Adapter B. Molybdate 3 reagent blank blank value, press the Install the Pour-Thru Cell by pressing existing value and adjust it with the 1-inch (round)
Page 731 Silica ULR(3 to 1000 µg/L as SiO 13. Press 14. When the timer 15. After the flow stops, 16. Add 1.0 mL of Amino TIMER>OK expires, pour the contents press Acid F Reagent to the ZERO A one-minute reaction of one flask into the remaining flask.
Page 732 Silica ULR(3 to 1000 µg/L as SiO Allow the sample stream to flow for 1–2 minutes before collection. Do not adjust the flow during the sampling period as this may introduce particulates. Rinse the container well with sample before collecting the portion for analysis. Analyze as soon as possible. Reagent Preparation Dissolve the contents of one bottle of Amino Acid F Reagent Powder in one bottle of Amino Acid Reagent Dilution Solvent.
Page 733 Silica ULR(3 to 1000 µg/L as SiO 5. Analyze each sample spike as described in the procedure above, starting with the 0.1 mL sample spike. Accept each standard additions reading by pressing . Each addition READ should reflect approximately 100% recovery. 6.
Page 734 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 735: Powder Pillows
Silica Heteropoly Blue Method Method 8186 Powder Pillows LR (0.010 to 1.600 mg/L as SiO Scope and Application: For water and seawater Adapted from Standard Methods for the Examination of Water and Wastewater. Test Preparation Before starting the test: The four-minute reaction time in step is for samples at 20 °C;...
Page 736 Silica LR (0.010 to 1.600 mg/L as SiO 5. Press 6. When the timer 7. Press 8. Prepared Sample: TIMER>OK TIMER>OK expires, add the contents When the timer expires, A four-minute reaction A one-minute reaction of one Citric Acid Reagent add the contents of one period will begin.
Page 737 Silica LR (0.010 to 1.600 mg/L as SiO Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels and Treatments Color Eliminated by zeroing the instrument with the original sample. Iron Large amounts interfere. Does not interfere at levels less than 50 mg/L PO .
Page 738 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 739 Silica Silicomolybdate Method Method 8185 Powder Pillows HR (1 to 100 mg/L) Scope and Application: For water and seawater Test Preparation Before starting the test: Sample temperature should be 15–25 °C (59–77 °F) Collect the following items: Quantity High Range Silica Reagent Set Deionized Water 10 mL Sample Cell, 1-inch square, 10-mL...
Page 740 Silica HR (1 to 100 mg/L) 5. Add the contents of 6. Press 7. When the timer 8. Press TIMER>OK TIMER>OK one Acid Reagent Powder expires, add the contents A ten-minute reaction A two-minute reaction Pillow for High Range of one Citric Acid Powder period will begin.
Page 741 Silica HR (1 to 100 mg/L) Occasionally a sample contains silica which reacts very slowly with molybdate. The nature of these “molybdate-unreactive” forms is not known. A pretreatment with Sodium Bicarbonate*, then Sulfuric Acid† will make these forms reactive to molybdate. The pretreatment is given in Standard Methods for the Examination of Water and Wastewater under Silica-Digestion with Sodium Bicarbonate.
Page 742 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 743 Silver Colorimetric Method Method 8120 Powder Pillows (0.005 to 0.700 mg/L) Scope and Application: For water and wastewater. Test Preparation Before starting the test: Digestion is required for samples with interferences. See Digestion on page For more accurate results, determine a reagent blank value for each new lot of reagent. Follow the procedure using deionized water instead of the sample.
Page 744: Stored Programs
Silver (0.005 to 0.700 mg/L) Powder Pillows Method 8120 1. Press 2. Select the test. 3. Add the contents of 4. Add the contents of one Silver 1 Powder Pillow one Silver 2 Reagent STORED PROGRAMS to a dry 50-mL graduated Solution Pillow to the mixing cylinder.
Page 745 Silver (0.005 to 0.700 mg/L) 9. Press 10. Pour 10 mL of the 11. When the timer 12. Press TIMER>OK. ZERO blank into a second square expires, insert the blank A two-minute reaction The display will show: sample cell. into the cell holder with the period will begin.
Page 746 Silver (0.005 to 0.700 mg/L) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels Mercury Positive interference above 2 mg/L Nickel Negative interference above 19 mg/L Zinc Negative interference above 70 mg/L Sample Collection, Storage, and Preservation Collect samples in acid-cleaned glass or plastic bottles. Using pH paper, adjust the pH to 2 or less with concentrated Nitric Acid* (about 2 mL/liter).
Page 747 Silver (0.005 to 0.700 mg/L) 2. To adjust the calibration curve using the reading obtained with the 0.5-mg/L silver standard solution, press on the current program menu. Press OPTIONS>MORE STANDARD ADJUST 3. Press . Press to accept the displayed concentration. If an alternate concentration is used, press the number to enter the actual concentration.
Page 748 Silver (0.005 to 0.700 mg/L) 10. Add another 5 mL of hydrogen peroxide. Check the solution for digestion completion. If digestion is not complete, continue adding hydrogen peroxide in 5 to 10 mL portions. Several portions may be necessary. Note: Digestion is complete when the digestate is colorless or the color of the digestate does not change upon addition of hydrogen peroxide.
Page 749 Silver (0.005 to 0.700 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Silver Reagent Set (50 tests), includes: 22966-00 Silver 1 Reagent Powder Pillow 50/pkg 22935-66 Silver 2 Reagent Solution Pillow 50/pkg 22936-66 Sodium Thiosulfate Powder Pillow 50/pkg 22937-66 Required Apparatus...
Page 750 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 751 Sulfate SulfaVer 4 Method Method 8051 ® Powder Pillows or AccuVac Ampuls (2 to 70 mg/L) Scope and Application: For water, wastewater, and seawater; USEPA accepted for reporting wastewater analyses Adapted from Standard Methods for the Examination of Water and Wastewater. Procedure is equivalent to USEPA method 375.4 for wastewater.
Page 752 Sulfate (2 to 70 mg/L) Powder Pillows Method 8051 1. Press 2. Select the test. 3. Fill a square sample 4. Prepared Sample: cell with 10 mL of sample. Add the contents of one STORED PROGRAMS SulfaVer 4 Reagent Powder Pillow to the sample cell.
Page 753 Sulfate (2 to 70 mg/L) AccuVac Ampul Method 8051 1. Select the test. 2. Insert Adapter C. 3. Prepared Sample: 4. Quickly invert the Collect at least 40 mL of Ampul several times to sample in a 50-mL beaker. mix. Fill a SulfaVer 4 Sulfate White turbidity will form if AccuVac Ampul with...
Page 754 Sulfate (2 to 70 mg/L) Sample Collection, Storage, and Preservation Collect samples in clean plastic or glass bottles. Samples may be stored up to 7 days by cooling to 4 °C (39 °F) or lower. Warm to room temperature before analysis. Accuracy Check Standard Additions Method (Sample Spike) 1.
Page 755 Sulfate (2 to 70 mg/L) Calibration Standard Preparation To perform a sulfate calibration using the SulfaVer method, use Class A glassware to prepare 2– calibration standards containing 10, 20, 30, 40, 50, 60, and 70 mg/L SO as follows: 1. Into seven different 100-mL Class A volumetric flasks, pipet 1, 2, 3, 4, 5, 6, and 7 mL of the 1000-mg/L Sulfate Standard Solution.
Page 756 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 757 Sulfide Methylene Blue Method Method 8131 (5 to 800 µg/L) – Scope and Application: For testing total sulfides, H S, HS , and certain metal sulfides in groundwater, wastewater brines, and seawater; USEPA Approved for reporting wastewater analysis Adapted from Standard Methods for the Examination of Water and Wastewater. 2–...
Page 758 Sulfide (5 to 800 µg/L) 5. Use the calibrated 6. Use the calibrated Cap the cell and 8. Press TIMER>OK dropper to add 0.5 mL of dropper to add 0.5 mL of immediately invert to mix. A five-minute reaction Sulfide 1 Reagent to each Sulfide 2 Reagent to each A pink color will develop, period will begin.
Page 759 Sulfide (5 to 800 µg/L) Table 1 Interfering Substances and Levels (continued) Interfering Substance Interference Levels and Treatments For turbid samples, prepare a sulfide-free blank as follows. Use it in place of the deionized water blank in the procedure. Measure 25 mL of sample into a 50-mL Erlenmeyer flask. Turbidity Add Bromine Water dropwise with constant swirling until a permanent yellow color just appears.
Page 760 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 761 Sulfite Colorimetric Method (0.10 to 5.00 mg/L) Scope and Application: For boiler water, foodstuffs Reagent sets for this method are only available in Europe. Test Preparation Before starting the test: Samples must be analyzed immediately. Sample and Reagent temperature must be between 15–25 °C (59–77 °F). Adjust the sample pH between 3–10.
Page 762 Sulfite (0.10 to 5.00 mg/L) 5. Add 5 drops of Sulfite 6. Add 2 drops of 7. Press 8. Wipe the blank and TIMER>OK Reagent A (HPT 430 A) to Sulfite Reagent B insert the it into the cell A 3-minute reaction period the prepared sample.
Page 763 Sulfite (0.10 to 5.00 mg/L) Interferences Table 1 Interfering Substances and Levels Interfering Substance Interference Levels Sulfide Greater than 5 mg/L Sample Collection, Storage, and Preservation Collect samples in clean plastic or glass bottles. Samples may be stored up to 7 days by cooling to 4 °C (39 °F) or lower.
Page 764 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 765: Surfactants, Anionic (Detergents)
Surfactants, Anionic (Detergents) Crystal Violet Method Method 8028 (0.002 to 0.275 mg/L as LAS) Scope and Application: For water, wastewater, and seawater Analytical Chemistry, 38, 791 (1966). Test Preparation Before starting the test: Use benzene only in a well-ventilated area. Benzene (D018) solutions are regulated as hazardous waste by the Federal RCRA.
Page 766 Surfactants, Anionic (Detergents) (0.002 to 0.275 mg/L as LAS) Method 8028 1. Press 2. Select the test. 3. Fill a clean 500-mL 4. Add 10 mL of Sulfate graduated cylinder to the Buffer Solution. Stopper STORED PROGRAMS 300 mL mark with sample. the funnel.
Page 767 Surfactants, Anionic (Detergents) (0.002 to 0.275 mg/L as LAS) 9. After the timer expires, 10. Prepared Sample: 11. Blank Preparation: 12. Insert the blank into remove the stopper and Drain the top benzene Fill another sample cell to the cell holder with the fill drain the bottom water layer into a clean 25-mL the 25-mL mark with pure...
Page 768: Accuracy Check
Surfactants, Anionic (Detergents) (0.002 to 0.275 mg/L as LAS) Accuracy Check Standard Additions Method (Sample Spike) 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. Verify that the units displayed are in mg/L. 2. Press .
Page 769 Surfactants, Anionic (Detergents) (0.002 to 0.275 mg/L as LAS) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Detergents Reagent Set, includes: — — 24468-00 (1) Benzene, ACS 55 mL 4 liters 14440-17 (2) Buffer Solution, sulfate-type 10 mL 500 mL 452-49 (3) Detergent Reagent Powder Pillows...
Page 770 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 771: Suspended Solids
Suspended Solids Photometric Method Method 8006 (5 to 750 mg/L) Scope and Application: For water and wastewater Adapted from Sewage and Industrial Wastes, 31, 1159 (1959). Test Preparation Collect the following items: Quantity Beaker, 600-mL, polypropylene Blender Cylinder, 500-mL polypropylene, graduated Pipet, serological, 25-mL Pipet Filler, safety bulb Sample Cells, 1-inch square, 10 mL...
Page 772: Sampling And Storage
Suspended Solids (5 to 750 mg/L) 5. Prepared Sample: 6. Blank Preparation: 7. Insert the blank 8. Press ZERO Stir the sample and Fill a second sample cell into the cell holder with the The display will show: immediately pour 10 mL of with 10 mL of tap water or fill line facing right.
Page 773 Suspended Solids (5 to 750 mg/L) Summary of Method This method of determining suspended solids is a simple, direct measurement which does not require the filtration or ignition/weighing steps that gravimetric procedures do. The USEPA specifies the gravimetric method for solids determinations, while this method is often used for checking in-plant processes.
Page 774 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 775: Tannin And Lignin
Tannin and Lignin Tyrosine Method Method 8193 (0.1 to 9.0 mg/L) Scope and Application: For water, wastewater, and boiler water Adapted from Kloster, M.B., Journal American Water Works Association, Vol. 66, No. 1, p. 44 (1974) Test Preparation Before starting the test: Filter turbid samples and report results as mg/L soluble tannic acid.
Page 776 Tannin and Lignin (0.1 to 9.0 mg/L) 5. Pipet 5.0 mL of 6. Pour 10 mL of each 7. Press 8. When the timer TIMER>OK Sodium Carbonate solution into two square expires, insert the blank A 25-minute reaction Solution into each cylinder. sample cells.
Page 777 Tannin and Lignin (0.1 to 9.0 mg/L) Sample Collection, Storage, and Preservation Collect samples in clean plastic or glass bottles. Accuracy Check Standard Solution Method Prepare a 200-mg/L tannic acid standard solution as follows: 1. Dissolve 0.200 grams of tannic acid in deionized water and dilute to 1000 mL. Prepare this solution monthly.
Page 778 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 779 Toxicity 1, 2 ,3 ToxTrak™ Method Method 10017 (0 to 100% Inhibition) Scope and Application: For drinking water, wastewater and natural waters U.S. Pat. No. 5,413,916 Liu, D., Bull. Environ. Contm. Toxicol. 26, 145-149 (1981) Environmental Technology Verification ETV Program evaluated, November, 2003 Test Preparation Before starting the test: Important Note: Install the light shield in Cell Compartment #2 before performing this test.
Page 780 Toxicity (0 to 100% Inhibition) Inoculum Development Using Indigenous Biomass 1. Using one of the 2. Incubate the tube dropper pipets provided, contents at 35 °C (95 °F) add 1.0 mL of source until the medium is visibly culture to a Total Bacteria turbid (approximately 12 Count Broth Tube.
Page 781 Toxicity (0 to 100% Inhibition) 5. Touch 6. Add 5.0 mL of 7. Add 5.0 mL of sample 8. Label a cell “control”. ZERO deionized water to the (or dilutions) to each Open one ToxTrak The display will show: control cell. sample cell.
Page 782 Toxicity (0 to 100% Inhibition) Repeat HRS MIN SEC HRS MIN SEC Step 12 13. Repeat step 12 for all 14. Allow the solutions in 15. After the absorbance 16. Press ZERO samples and dilutions. Be the tubes to react until the of the control has The display will show: sure to record each...
Page 783: Interpreting Results
Toxicity (0 to 100% Inhibition) Interpreting Results The percent Inhibition (%I) results obtained are only a relative measurement. They do not represent a true quantitative measurement of toxic concentration. The percent Inhibition does not necessarily increase in direct proportion to the concentration of toxins. To determine the minimum inhibition concentration of a toxin, it is possible to make tenfold dilutions of the sample and determine the percent Inhibition for the dilutions until the sample is diluted sufficiently so that no inhibition is observed.
Page 784 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 785 TPH (Total Petroleum Hydrocarbons) Immunoassay Method Method 10050 Scope and Application: For soil and water This test is semi-quantitative. Results are expressed as greater or less than the threshold value used. Test Preparation This TPH test can be used for both soil and water testing. When testing soil, perform the Soil Extraction Procedure on page 2.
Page 786 TPH (Total Petroleum Hydrocarbons) Soil Extraction Procedure 1. Weigh out 5 g of soil in 2. Carefully pour the soil 3. Use the 5-gram scoop 4. Use the graduated the plastic weighing boat. into an extraction vial. to add one scoop of cylinder to transfer 10 mL sodium sulfate to the of Soil Extractant into the...
Page 787 TPH (Total Petroleum Hydrocarbons) Immunoassay for Soil Extracts and Water Samples Label an Antibody Cuvette 1. Press 2. Insert Adapter A. 3. Insert the cuvettes into for each calibrator and the rack snugly. SINGLE each sample to be tested. WAVELENGTH>OPTIONS To select the proper λ...
Page 788 TPH (Total Petroleum Hydrocarbons) HRS MIN SEC HRS MIN SEC 8. Immediately pipet 9. Press . Enter 10. After 5 minutes, mix 11. At the end of the TIMER 0.5 mL of TPH Enzyme minutes and press the contents of the rack a 10-minute period, discard 10:00 Conjugate into each...
Page 789 TPH (Total Petroleum Hydrocarbons) Measuring the Color 16. At the end of the 17. Label and fill a Zeroing 18. Insert the filled zeroing 19. Insert the first 10-minute reaction period, Cuvette with deionized cuvette into the cell holder calibrator into the cell pipette 0.5 mL of Stop water.
Page 790 TPH (Total Petroleum Hydrocarbons) ® Using the Wiretrol * Pipet The Wiretrol Pipet can accurately measure small quantities of liquids. It consists of two parts: ® a Teflon -tipped plunger and a calibrated capillary tube. The plunger can be reused; the capillary tubes must be discarded after one use..
Page 791 TPH (Total Petroleum Hydrocarbons) Loading the Rack—The cuvette rack is designed so that it may be inverted with the cuvettes in place. Identify each cuvette with a sample or calibrator number and insert all the cuvettes in the rack before beginning the procedure. Fit the cuvettes snugly into the rack, but do not force them or they may be difficult to remove and their contents may spill.
Page 792 TPH (Total Petroleum Hydrocarbons) • Keep the foil pouch containing the Antibody Cuvettes sealed when not in use. • If Stop Solution comes in contact with eyes, wash thoroughly for 15 minutes with cold water and seek immediate medical help. Sensitivity The antibodies used in the TPH Test Kit react with a variety of compounds found in petroleum fuels;...
Page 793 TPH (Total Petroleum Hydrocarbons) conversion factors given in Table 2 Table 3. Dilute the sample to 50 mL with deionized water in a graduated cylinder. Choose the mL of sample from Table 4. Use the multiplier value for the chosen quantity to multiply the value from Table Table 4 Sample Multipliers...
Page 794 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 795 Trihalomethanes THM Plus™ Method Method 10132 Water Bath Method (10 to 600 ppb as Chloroform) Scope and Application: For screening THMs in drinking water samples and Formation Potential tests. Test Preparation Before starting the test: If analyzing more than four samples, use 450 mL of water in the water bath. THM Plus Reagent 2 must be at room temperature before use.
Page 796: Stored Programs
Trihalomethanes (10 to 600 ppb as Chloroform) Method 10132 1. Press 2. Select the test. 3. Prepare a hot water 4. Prepare a cooling bath bath by adding 500 mL of by adding 500 mL of cold STORED PROGRAMS. water to an evaporating (18–25 °C) tap water to a dish.
Page 797 Trihalomethanes (10 to 600 ppb as Chloroform) ® 9. Use a TenSette Pipet 10. Cap tightly and mix by 11. Place the sample cells 12. Place the assembly in to add 3 mL of THM Plus shaking. in the cell holder assembly. the hot water bath when Reagent 2 to each cell.
Page 798 Trihalomethanes (10 to 600 ppb as Chloroform) 10 mL 17. Replace the cooling 18. Press 19. Add the contents of 20. Cap each cell tightly TIMER>OK water with fresh, cold tap one THM Plus Reagent 4 and mix by shaking until all A second three-minute water.
Page 799 Trihalomethanes (10 to 600 ppb as Chloroform) Interferences The substances in Table 1 have been tested and found not to interfere up to the indicated levels (in ppm): Table 1 Interferences That Have No Effect Up to the Maximum Level Tested Interfering Substance Interference Levels and Treatments Chlorine...
Page 800: Accuracy Check
Trihalomethanes (10 to 600 ppb as Chloroform) Accuracy Check Standard Additions Method (Sample Spike) Prepare the standard additions sample at the same time as the unspiked water sample. 1. Snap the neck off a THM Standard Ampule, 10 ppm as chloroform. 2.
Page 801 Trihalomethanes (10 to 600 ppb as Chloroform) The sample is then cooled and acidified to pH 2.5. The dialdehyde intermediate formed is then reacted with 7-amino-1,3 napthalene disulfonic acid to form a colored Schiff base. The color formed is directly proportional to the total amount of THM compounds present in the sample. Test results are measured at 515 nm and reported as ppb chloroform.
Page 802 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 803: Volatile Acids
Volatile Acids Esterification Method Method 8196 Powder Pillows (27 to 2800 mg/L) Scope and Application: For digestor sludges Adapted from The Analyst, 87, 949 (1962) Test Preparation Collect the following items: Quantity Centrifuge Centrifuge Tubes and Caps Cylinder, 10-mL graduated Ethylene Glycol 3 mL Ferric Chloride-Sulfuric Acid Solution...
Page 804 Volatile Acids (27 to 2800 mg/L) Esterification Method 8196 1. Select the test. 2. Blank Preparation: 3. Filter or centrifuge 4. Prepared Sample: Pipet 0.5 mL of deionized 25 mL of sample. Pipet 0.5 mL of the filtrate water into a dry 25-mL Centrifuging is faster than or supernatant into a sample cell.
Page 805 Volatile Acids (27 to 2800 mg/L) 13. Add 10 mL of 14. Transfer 10 mL of the 15. Transfer 10-mL of the 16. Immediately press deionized water to each blank solution from the sample solution from the TIMER>OK cell. Cap and invert to mix. round 25-mL cell to a round 25-mL cell to a Another three-minute...
Page 806 Volatile Acids (27 to 2800 mg/L) ® 4. Snap the neck off a Volatile Acid Voluette Ampule Standard, 62,500-mg/L as acetic acid. 5. Prepare three sample spikes. Fill three Mixing Cylinders* with 25 mL of sample. Use the ® TenSette Pipet to add 0.1 mL, 0.2 mL, and 0.3 mL of standard, respectively, to each sample and mix thoroughly.
Page 807 Volatile Acids (27 to 2800 mg/L) Consumables and Replacement Items Required Reagents Description Quantity/Test Unit Cat. No. Volatile Acid Reagent Set (90 tests), includes: 22447-00 (1) Ethylene Glycol 3 mL 1000 mL 2039-53 (2) Ferric Chloride-Sulfuric Acid Solution 20 mL 1000 mL 2042-53 (1) Hydroxylamine Hydrochloride Solution, 100-g/L...
Page 808 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...
Page 809 Zinc Zincon Method Method 8009 Powder Pillows (0.01 to 3.00 mg/L) Scope and Application: For water and wastewater; digestion is required for determining total zinc (see Digestion on page 4); USEPA Approved for wastewater analyses Adapted from Standard Methods for the Examination of Water and Wastewater. Federal Register, 45(105) 36166 (May 29, 1980).
Page 810 Zinc (0.01 to 3.00 mg/L) Powder Pillows Method 8009 CAUTION ZincoVer 5 Reagent contains cyanide and is very poisonous if taken internally or if fumes are inhaled. Do not add to an acidic sample (pH < 4). 1. Press 2. Select the test. 3.
Page 811 Zinc (0.01 to 3.00 mg/L) 9. Press 10. Pour the prepared 11. When the timer 12. Wipe the prepared TIMER>OK sample solution from the expires, wipe the blank sample and insert it into A three-minute reaction cylinder into a second and insert it into the cell the cell holder with the fill period will begin.
Page 812 Zinc (0.01 to 3.00 mg/L) Accuracy Check 1. After reading test results, leave the sample cell (unspiked sample) in the instrument. 2. Press . Press . A summary of the standard additions OPTIONS STANDARD ADDITIONS procedure will appear. 3. Press to accept the default values for standard concentration, sample volume, and spike volumes.
Page 813 Zinc (0.01 to 3.00 mg/L) 5. Filter cooled sample through a membrane filter and adjust the volume to 100 mL with Deionized Water. 6. Adjust the pH to 4–5 with 5.0 N Sodium Hydroxide* before analysis. See Sample Collection, Storage, and Preservation on page 3 for instructions.
Page 814 Outside the U.S.A. – Contact the HACH office or distributor serving you. Telephone: (970) 669-3050 On the Worldwide Web – www.hach.com ; E-mail – techhelp@hach.com FAX: (970) 669-2932 © Hach Company, 2007. All rights reserved. Printed in Germany. Updated June 2007 Edition 2...