HP D300 Operating Manual page 40

3. In the Fluid drop-down list box, select a fluid to use for normalization.
Figure 5-11 DMSO Normalize dialog box
Type a value in the box following ―DMSO normalize to‖, or you can choose to normalize up to
4.
the highest % DMSO already programmed into any of the selected wells, by selecting the
Backfill only up to the highest-filled well option.
The maximum normalization range is determined by the DMSO Limit % entered for the
selected fluid in the Fluids List. The minimum normalization range is determined by maximum
amount of DMSO already present in the selected wells. You can enter a normalization
percentage below the minimum valid range; see the note below.
TIP: You may enter a normalization percentage that is less than the valid normalization range,
however all wells will not be normalized to the same DMSO percentage. For example, entering
0.1% DMSO normalization will only add DMSO to wells which are less than 0.1% DMSO, and any
wells which are greater than 0.1% DMSO will have no additional DMSO added to them.
TIP: To ensure proper DMSO normalization volumes, perform the DMSO normalization function
after configuring the wells with all fluids. To add another fluid to wells which already had the
normalization operation applied to them, delete the DMSO normalization fluid from those wells by
selecting the appropriate wells, right click and select Remove Fluid, then pick the DMSO from the
fluid drop down. After removing normalization, add the new fluid in the appropriate wells before
repeating the DMSO normalization step.
HP D300 Digital Dispenser 40