HP D300 Operating Manual page 36

3. To generate a sequence of concentrations or volumes in the selected wells (a titration series,
with different concentrations and volumes in each well), right-click anywhere in the well plate
image and click Set Titration. The Set Titration dialog box opens (see
concentration or volume is used in this dialog depends on the setting chosen in the Edit: Units
pane of the Options dialog box (see
Select Zig-Zag, Snake or Random:
a.
○
Zig-Zag builds the titration one row or column at a time (depending on whether
Horizontal is selected in the Direction area), then starts the next row or column in a well
adjacent to the first well.
○
Snake builds the titration continuously, so that every well of the titration is adjacent to
the wells closest in concentration or volume.
○
Random takes the set of concentrations or volumes and distributes them randomly
among the selected wells. Randomization of the entire plate can also be accomplished
later, using the Randomize function as described in the ―Randomize‖ section.
Select the direction that the titration will be built. These choices are not available for
b.
random titrations. Note that the arrows and shadings of the graphic in this window change
as you make different selections to help you understand the options.
○
Horizontal: This option creates doses from left to right. If this box is not checked, the
default is a vertical dose order (top to bottom).
○
Reverse: This option reverses the concentration or volume order from high-to-low to low-
to-high. If this box is not checked, the default is a high-to-low titration.
○
Mirror: This option flips the titration either horizontally or vertically depending on whether
the Horizontal check box is selected. The easiest way to understand this is to select the
Mirror check box and observe what happens to the displayed graphic.
Select the fluid to be programmed in the Fluid drop-down list box.
c.
Select the type of concentration or volume titration in the General Type area of the dialog
d.
box. Select either Linear or Logarithmic.
○
Linear: The titration steps are evenly spaced in concentration or volume units
○
Logarithmic: The titration steps are evenly spaced in log concentration or log volume
units
Figure 4-12).
HP D300 Digital Dispenser
Figure 5-6). Whether
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