Options: Edit: Assay Defaults; Options: Plate Jitter - HP D300 Operating Manual

4-4-4 Options: Edit: Assay Defaults

In the Options dialog box, click Edit and then Assay Defaults to open the Edit: Assay Defaults
pane of the Options dialog box. Set the default assay volume and the default DMSO limit here.
See Figure 4-14.
Figure 4-14 Edit: Assay Defaults pane of the Options dialog box

4-4-5 Options: Plate Jitter

In the Options dialog box, click Plate Jitter to open the Plate Jitter pane of the Options dialog box.
See Figure 4-15. Use plate jitter if shaking the destination plate is desired either during and/or after
dispense, for example if you are dispensing into wells containing cells.
Select Jitter the plate while dispensing into each well to shake the destination plate during the
dispense. When this option is chosen, shaking only above a specified volume may be entered.
Typically, only dispense volumes greater than 100nL have a deleterious effect on plated cells.
Additionally, Jitter the plate after dispensing from each Dispensehead may be selected to shake
the destination plate post-dispense. If this option is utilized, enter the number of seconds to shake
after each Dispensehead has completed dispensing.
IMPORTANT:
plates containing live cells to reduce the deleterious effect of a DMSO bolus on the cells.
Plate Jitter while dispensing is highly recommended when dispensing directly into
HP D300 Digital Dispenser 19