Table of Contents
Advertisement
4.
Put at least one fluid in the Fluids List, by entering any characters in the Name cell, or by
copying and pasting a list of compounds from an Excel file. The order of the fluids in the Fluids
List is the order in which the fluids will be dispensed.
5.
Learn how to select and deselect wells in the plate design:
○
Select any number of wells by clicking and dragging in the well plate image. Any well
which is partially selected becomes one of the selected wells. Give it a try!
○
Select nonadjacent wells by holding down the CTRL key while selecting wells.
○
Deselect wells by clicking on them again while holding down the CTRL key.
○
Deselect all selected wells by clicking on other wells or clicking on the edge of the well
plate (outside the wells) while not holding down the CTRL key.
6.
Just for fun, select some wells, then right-click on them to open the Edit Well Plate menu. Click
on Set Volume or Set Concentration, enter the number 1 in the volume or concentration box
and click OK. The wells you selected will turn red because they now contain fluid 1 in your
plate design.
In general, this is a great way to learn to use the software—try things out, right- or left-click
when you are unsure, and move the mouse over areas to check them out.
Don't worry, you won't break anything!
NOTE:
Either Set Volume or Set Concentration is available on the well plate options list
depending on whether Volume or Concentration mode was selected in the Options: Edit:
Units. See
TIP:
If the Set Volume or Set Concentration option is grayed out, the fluids table is empty, so
follow step
7.
Now that you have set up some wells, place the cursor over a colored well and observe the
information that appears in the well pop-ups.
Note that the first three values (assay volume, total volume, and DMSO content) regard the
entire contents of the well. Below these values, information is given separately for each fluid in
that well. With the exception of assay volume, there are two values for each well pop-up entry:
the first value and then a second value enclosed by square brackets, for example [0.960 nl].
The first value is the volume or concentration that was requested, and the second bracketed
value is what will actually be dispensed since only an integer number of drops can be
dispensed. This is where the name Digital Dispenser comes from, an integer number of
ultrasmall droplets.
NOTE:
Two different drop volumes are used in the dispenser. For dispense volumes less than
3.5nL, the dispenser uses the closest number of either 13pL or 20pL drops to dispense as close
as possible to the requested volume. For volumes greater than 3.5nL, the dispenser uses the
closest number of 400pL bursts (20 drops simultaneously dispensed from 20 nozzles which are
each 20pL) in order to dispense very close to the requested volume very rapidly.
4-3 Load an existing plate configuration
NOTE:
To build a new plate configuration, see
―Options: Edit:
Units‖ for how to set Volume or Concentration mode.
4
above to add a fluid to the fluid table.
HP D300 Digital Dispenser
―Create plate
configurations‖.
14
Hide quick links:
Advertisement
Table of Contents
