Load Fluid Into The Dispensehead - HP D300 Operating Manual

Lift a new Cassette out of the clamshell container and place it white logo side up into the
b.
cutout on the Dispenser deck.
Make sure it is fully inserted so the top surface of the Cassette is the same height as the
c.
top of the deck all the way around the cassette.
Figure 4-21
3. Once the Cassette is fully inserted, click OK in the Load Cassette dialog box (see
20). The pogo mechanism will engage and the Dispenseheads will be electrically tested to
ensure quality. Then the Load Fluid dialog box appears. See

4-5-4 Load fluid into the Dispensehead

When the Load Fluid dialog box opens, the OK and Skip buttons flash above the first Dispensehead
that will be loaded (see
of the pogo mechanism.
1. Fill a low volume pipette with at least the amount of fluid that is indicated in the Load Fluid
dialog box, or fill a multipipetter with the appropriate number of fluids in the proper order as
shown in the Load Fluids dialog box. If a multipipetter is used, use the Max. Load Volume for
all Dispenseheads as shown on the right side of the dialog, as circled in
source plate and source well location of the fluid to load will also be identified in the Load Fluid
dialog box if that information was entered in the Fluids List. Be sure to load the proper
compound into the proper Dispensehead.
CAUTION: Using a multichannel pipette enables the loading of multiple Dispenseheads
simultaneously. Make sure that you fill the Dispenseheads with the LARGEST load volume of the
set of Dispenseheads you are loading. It is indicated in the upper right of the dialog box (―Max.
Load Volume‖) as shown in
Loading a new Dispensehead Cassette
Figure 4-22), and a blue LED illuminates the first Dispensehead on the front
Figure 4-22.
HP D300 Digital Dispenser
Figure 4-
Figure 4-22.
Figure 4-22. The
23