HP D300 Operating Manual page 45

3. Notice the left pane of the Plate Layout Wizard: Titrations and Positioning dialog box.
Recall that a titration is simply a set of concentrations or volumes that form a titration. Two
types of titrations can be programmed:
○
Titration1 is denoted as Titration1 in the menu in the left pane, and is used for titration
series with one fluid per well.
○
Titration2 is denoted as Titration2 and is used for titration series with two fluids per well.
Plate designs can have zero or many titration1s and/or titration2s on a plate. Each titration1
can use from one to all of the fluids in the fluids table. If all fluids in the plate will have the
same set of concentrations or volumes, only one titration1 is required. Additional titration1s
are only needed in order to use a different set of concentrations or volumes for one or more
fluids.
By default, designs start with the definition of any titration1 fluids. If the desired plate
a.
design involves only titration2 fluids, click Titration2: <none> in the left pane, or click the
Next button to move down the list to the next item. Click the Back and Next buttons to go
up or down the list as desired.
Figure 5-18 Plate Layout Wizard Titration1 fluid selection pane
Select the Use check box next to each fluid that will use this titration to create a titration1
b.
that will be used by one or more fluids. Click on the All button to use all fluids in the fluids
table with the same titration. Select None to uncheck all of use boxes at the same time.
To use all fluids except for a few, select All, then click on the Use check boxes to uncheck
just those few fluids to not use. When one or more Use checkboxes are selected, the
appearance of the dialog box changes as shown below in
HP D300 Digital Dispenser
Figure 5-19.
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