Transmitted Light Experiments - Molecular Devices ImageXpress Micro User Manual

ImageXpress Micro High Content Imaging System Options User Guide
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Transmitted Light Experiments

Do the following procedure if you are running an experiment with
Transmitted Light as one of the selected wavelengths.
Transmitted light acquisition is compatible with the confocal modes in
the ImageXpress Micro Confocal System.
To run a Transmitted Light experiment
1. If you are running a phase-contrast experiment, make sure a
phase-contrast objective is selected. Phase-contrast objectives
are not required for brightfield experiments.
2. Make sure the annulus on the Transmitted Light arm matches the
objective.
3. Load a suitable plate.
The plate must be unlidded, have a clear lid, or have a clear plate
seal on the top. Condensation on a lid or plate seal can
negatively affect image quality.
4. Turn on the Transmitted Lamp by selecting the appropriate
Illumination setting in the Configure Illumination dialog.
5. In the MetaXpress software, click Screening > Plate Acquisition
Setup.
6. In the Plate Acquisition Setup dialog, define one of the
wavelengths as Transmitted Light.
7. Configure the remaining settings as usual.
8. Run the acquisition.
Tip: To increase the life of the halogen lamp, turn off the lamp when
the transmitted light unit is not in use by selecting the Transmitted
Lamp Off setting in the Configure Illumination dialog.
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