Molecular Devices ImageXpress Micro User Manual page 39

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12. On the Fluidics System Properties dialog, leave the default
values for the fluidic system properties set as-is, or modify any
values as needed.
Table 2-1: Fluidic Systems Property values
Option
Draw Rate (μl/s)
Dispense Rate (μl/s)
Post Draw Transport
Air Gap (μl)
Pre Draw Air Gap (μl)
Wet Dispense
Smart Dispense (μl)
Draw Overfill (μl)
Pump Settle Time (ms) The time delay that is used to pause
Fluidics Option Operations
Description
The rate at which the pump draws fluid.
The rate at which the pump dispenses fluid.
The volume of air that the pump draws after
it draws fluid but before the pump arm
moves. This air prevents fluid from dripping
during pump arm movement. Select this
option for fluids such as DMSO.
The volume of air that the pump draws
before drawing fluid. This is used to push
remaining drops of fluid out of the tip when
dispensing.
Specifies whether the tip should be
immersed in the fluid during dispense
operations at the imaging location. When
enabled, if Track Liquid Surface is also
selected. then dispensing occurs near the
liquid surface; otherwise, the dispensing
occurs near the bottom of the well.
Enabled only if Wet Dispense is selected. The
volume that the pump dispenses beyond the
requested fluid dispense volume. The
additional volume is subtracted from the Pre
Draw Air Gap volume. The Smart Dispense
volume must be less than or equal to the Pre
Draw Air Gap volume.
The volume of fluid that the pump draws in
addition to the requested volume of fluid.
You can use Draw Overfill to increase
dispense accuracy when drawing from
compound plates.
Note: Draw Overfill is not applicable for any
other plate type.
movement of the pump arm after a pump
draw or dispense operation to overcome
hysteresis. For most operations, 100 ms is
the recommended value.
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