Molecular Devices ImageXpress Micro User Manual page 71

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12. Use the region tools in the MetaXpress software to mark the
position of the phase ring. Make sure that you capture both
horizontal and vertical axes. Use either the ellipse region tool or
multiple line tools. See
Figure 4-20: Marking the position of the phase ring
13. In the MetaXpress software, click Regions > Save Regions to save
the marked positions.
14. Manually rotate the condenser turret to the appropriate annulus
for the objective (PhL for 4x, Ph1 for 10x and 20x, Ph2 for 40x).
The selected annulus is at the front of the condenser turret.
Phase contrast objectives typically have their specifications
indicated in green letters on the outer barrel. These
specifications also include the matching annulus designation (for
example, PhL, Ph1, Ph2).
Transmitted Light Option Operations
Figure 4-20.
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