About Tuning; Autotune And Checktune - Agilent Technologies 6475 User Manual

3 Tuning

About Tuning

About Tuning

Autotune and Checktune

A Checktune can be used to determine if the tuning mix ion masses are properly
assigned and if the response or sensitivity of these ions is within expectations. In
other words, a Checktune verifies peak width and mass axis to make sure they
are correct before you start your acquisition. This check tune takes
approximately 3 minutes to run in each polarity. See
(Checktune)"
Autotune only needs to run after preventative maintenance or if you find a
problem with Checktune. You only mark Tune from factory default if regular
Autotune fails, or if the tune file was corrupted. An Autotune can take
approximately 15 to 20 minutes for each polarity. Everything is automatic since
the tuning mix is delivered by the calibrant delivery system, which is switched on
automatically during the tune. See
page 31.
Agilent 6475 Triple Quad LC/MS System User Guide
on page 30.
"Calibrating the LC/TQ (Autotune)"
"Calibrating the LC/TQ
on
28