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Use this guide for your first steps with the Agilent 6400 Series Triple Quad LC/MS, and as a
road map for your user information.
What is the Agilent 6400 Series Triple Quad LC/MS system?
The Agilent Triple Quadrupole LC/MS is a liquid chromatograph triple quadrupole
mass spectrometer that performs MS/MS using three sets of parallel rods (in this
case, quadrupole, hexapole, quadrupole). The first quadrupole separates ions into
precursor ions that are fragmented in the hexapole into product ions, which are
separated by the second quadrupole. Often, two or more precursor ions and their
product ions are monitored in sequence in MRM (multiple reaction monitoring)
mode. You can monitor up to 4000 MRM transitions by using Dynamic MRM.
The Agilent 6490 and 6495 Triple Quadrupoles contain the iFunnel Technology
which provide more efficient sampling of ions into the mass spectrometer. The
iFunnel Technology includes the Dual Offset Ion Funnel and these other
technologies: the Agilent Jet Stream Technology, and shorter desolvation assembly
with Hexabore Capillary.
Agilent 6400 Series Triple Quadrupole
LC/MS System
Quick Start Guide
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Summary of Contents for Agilent Technologies 6400 Series
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Page 1: Table Of Contents
Step 5. Review results with the Qualitative Analysis program Step 6. Analyze data with the Quantitative Analysis program Use this guide for your first steps with the Agilent 6400 Series Triple Quad LC/MS, and as a road map for your user information. - Page 2 What’s New in Data Acquisition The Data Acquisition program for the 6400 Series Triple Quadrupole has many new features in this revision. in B.07.00 • The supported models are 6420A, 6430A, 6460A, 6460C, 6490A, and 6495A.
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Page 3: Where To Find Information
Concepts Guide - The Big Picture Learn the background information to help you understand operation of the hardware and software. Data Acquisition for 6400 Series Triple Quad Familiarization Guide Do the exercises to learn to use the Triple Quad LC/MS hardware and Data Acquisition program for 6400 Series Triple Quad. - Page 4 Use the quick start guides for Study Manager, Optimizer and Compliance to get to know these programs. Training Courses Visit www.chem.agilent.com to view a listing of training courses for the Agilent Triple Quad LC/MS. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
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Page 5: Getting Started
Acquisition Here! software. Prepare the Set up, run Triple Quad and analyze instrument. samples Analyze data Set up and Review with run an results with Quantitative acquisition Qualitative Analysis. method. Analysis. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide... -
Page 6: Step 1. Start The Data Acquisition Software
If you need to restart them, right-click the Acq System Launcher icon in the system tray, and click Start Engines. If you have recently changed LC modules, remember to configure the instrument again. See the Installation Guide for instructions. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide... - Page 7 The Sample Run and Worklist windows are tabbed here. These three windows are “sharing” this space. You click the tab to switch to a different window. Figure 1 Main window of the Data Acquisition software program Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 8 Color Coding in the Instrument Status Window Color Status Error Yellow Not ready Purple Pre-run, post-run, Waiting Blue Running, injecting Green Idle Dark gray Offline Light gray Standby (for example, lamps off) Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 9 You select the plots in the Chromatogram tab in the QQQ tab in the Method Editor window. Spectrum Plot window With this window you monitor the spectral plot in real time. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 10 Override DA Method and select either Both Acquisition and DA or DA Only for the Method Type, and then Data Analysis is run as part of the method. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 11 This section shows the current progress in the Autotune or Checktune. If you make changes in the Manual Tune tab, you can print a Checktune and Autotune report when you click Create Tune Report. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
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Page 12: Step 2. Prepare The Lc Modules
Right-click the QQQ device in the Instrument Status window. b Click LC > Waste. 2 Purge the LC pump. Follow the directions for purging the pump in the User Guide for your pump. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide... - Page 13 Monitor the baseline and adjust the plot to make sure the column is equilibrated and the baseline stable. (See step 4 step 5 page 14.) Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 14 You can also set a range for the parameters which are numbers. If the value of the parameter is not within the limits which you entered, then the background of the parameter is set to red. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 15 As you condition the column, you can set up the displays to monitor the effluent. • Right-click the chromatogram plot, and click Change. In the Edit Signal Plot dialog box, you can select the type of display signal. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
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Page 16: Step 3. Prepare The Triple Quad Instrument
You can run a Checktune with the following sources: ESI, AJS ESI, MMI and HPLC-Chip. If Checktune passes, then skip to step If Checktune fails, then you can try using the Manual Tune tab to fix the problem. See the next step. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide... - Page 17 Then, if you click Autotune, the instrument is tuned starting from the factory defaults (approximately 30 minutes for each polarity for all models except the 6490 and the 6495. The 6490 and 6495 take approximately 25 minutes per ionization mode.). Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 18 • After you condition the column and tune the Triple Quad MS, you switch the LC stream from Waste to MS. See “Switch LC stream to Waste.” on page 12 for how to do this. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 19 When the System Logbook Viewer is open, you can select the time period to keep System Logbook entries. You can set the value from 1 week to 1 year. To do this, you click Tools > Purge Settings. The System logbook purge dialog box opens. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
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Page 20: Step 4. Set Up And Run An Acquisition Method
2 Enter values for all of the LC modules configured for the instrument. Make sure when you type the MS parameters on the next page that the tune file is the one NO T E that you want to use with the acquisition. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide... - Page 21 To add a segment, right-click anywhere in the Scan segments section to bring up the Scan Segments shortcut menu, and click Add Row. b Type the parameters for each Scan segment. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 22 Select the Stored instrument curves. These curves can be shown in the Chromatogram Results window in the Qualitative Analysis program. The values can be seen in the MS Actuals window. 7 Set up the data analysis (DA) parameters. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 23 Template File to use. If you want to print the report, mark the Print to default printer check box. You can also mark the Publish check box to create a CSV file, a TXT file, or a PDF file. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 24 Enter the Sample Name, the Data File Name, the Path and other values. c Enter the Additional Information. You can change the value of the parameters in the Additional Information list. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 25 Enter all the information on the Sample Information tab. e Click the Sample Position tab to specify the sample vial locations (make sure the specific sample tray type has been configured by right-clicking the autosampler device image). Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
- Page 26 DA parameters you want to use for the sample. The DA part of this method is used instead of the DA part of the current method. You can also type the name of this method in the Add Multiple Samples dialog box. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide...
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Page 27: Step 5. Review Results With The Qualitative Analysis Program
Also, refer to the online Help for the Quantitative Analysis program to learn how to do more operations to analyze your data. Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide... - Page 28 • Set up acquisition methods. • Set up and run worklists. Microsoft®, Windows®, Windows 7® and Excel® are registered trademarks of Microsoft Corporation in the US and/or other countries. Agilent Technologies, Inc. 2014 Printed in USA Revision A, May 2014 *G3335-90168* G3335-90168...