Agilent Technologies 6475 User Manual page 69

5 Maintenance
Sources
Installing an Electrospray or Agilent Jet Stream source
1 Check that the appropriate capillary cap is installed.
2 Install the appropriate spray shield.
3 For Electrospray (ESI), do the following:
4 Install the spray chamber.
5 Close the spray chamber.
6 Connect the MultiMode cable delivering AJS nozzle voltage.
7 Connect the heater cable.
8 Connect the sample nebulizer gas tubing.
9 Connect the nebulizer sample line.
Do not hit the tip of nebulizer needle. The needle is not protected. If you
C AU T I O N
damage the needle, system performance can be
10 Connect the reference nebulizer sample line and sample nebulizer gas tubing.
In the Data Analysis program,
1 In the Tune context, select the new ion source.
2 Turn on the LC/MS instrument.
3 While you wait for the set points to equilibrate, prepare and install calibrant for
Agilent 6475 Triple Quad LC/MS System User Guide
a Make sure the small hole is at the top ("12 o'clock position").
b If needed, use a T10 Torx screwdriver to loosen the two screws in the end
plate.
c Rotate the ESI spray shield clockwise until the hole is in the correct
position.
d Gently tighten the Torx screws again.
the new ion source.
a Rinse a clean calibrant bottle with LC/MS-grade acetonitrile.
b Pour the ESI Calibrant into the calibrant bottle.
c Install the calibrant bottle into the calibrant delivery system.
affected.
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