Flow Cell Types - illumina MiSeq System User Manual

Flow Cell Types

The flow cell is immersed in storage buffer in a flow cell container. The flow cell container
cap color indicates the flow cell type:
}
Clear cap—Cap color for a standard (14 tile) flow cell.
}
Green cap—Cap color for a micro (4 tile) flow cell.
} Yellow cap—Cap color for a nano (2 tile) flow cell.
Flow cell types differ in output and number of tiles imaged. Dual-surface imaging, which is
possible on the standard and micro flow cells, doubles the number of tiles imaged.
Flow cell output depends on the number of reads possible from each flow cell. Reads are
measured as the number of clusters passing filter.
Flow Cell Output
Flow Cell
Standard Flow Cell 15 M
Micro Flow Cell
Nano Flow Cell
Flow Cell Tiles
Flow Cell
Standard Flow Cell
Micro Flow Cell
Nano Flow Cell
14
# of Kit Size
Reads (Cycles)
50, 300, 500
4 M 300
1 M 300, 500
Tiles Imaging Surface
14 tiles Top and bottom
4 tiles Top and bottom
2 tiles Top only
Output 2 x 75
(Max.) Output
7.5 Gb 2.25 Gb
1.2 Gb 600 Mb
500 Mb 150 Mb
Total Tiles Imaged
28 tiles total
8 tiles total
2 tiles total
2 x 150 2 x 250
Output Output
4.5 Gb 7.5 Gb
1.2 Gb --
300 Mb 500 Mb
Part # 15027617 Rev. H