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Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)
6
Fully open the field diaphragm
and aperture diaphragm of the
Epi-illumination attachment.
7
Switch on power for the light
source of the Epi-illumination
attachment, then open the
shutter and center the lamp.
(Refer to the operating manual
for the light source.)
8
Set the 10x objective into the
optical path.
9
Set a specimen and move the
portion to be viewed into the
optical path.
2.4 Epi-fluorescence Microscopy
32
Fully open the field
diaphragm.
Open the shutter.
Select the 10x objective.
Set a specimen and secure in place
using the specimen holder.
Move the portion to be viewed into the
optical path using the XY stage knobs.
Fully open the
aperture diaphragm.
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