Nikon ECLIPSE 80i Instructions Manual page 44

Microscope
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Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)
5
Rotate the excitation method
switchover turret to set it a
position where no fluorescent
cube is installed.
6
Raise the condenser to the
uppermost position.
7
Fully open the field diaphragm
and aperture diaphragm.
8
Set the 10x objective into the
optical path.
3.3 Bright-Field Microscopy
42
Rotate the excitation method
switchover turret.
Raise the condenser using the
condenser focus knob.
Fully open the aperture
diaphragm using the
aperture diaphragm
lever.
Select the 10x objective.
Fully open the field
diaphragm using the
field diaphragm knob.

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