Nikon ECLIPSE 80i Instructions Manual page 32

Microscope
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Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)
13
Switch to the desired objective
and view the specimen.
Adjust the field diaphragm and
aperture diaphragm each time
you change objectives.
Field diaphragm:
Set slightly narrower than the margins of
the field of view.
Aperture diaphragm:
70% to 80% of the maximum numerical
aperture (N.A.) of the objective
14
Turn off power after completing
observations.
2.3 Bright-Field Microscopy
30
Select the desired objective.
Field diaphragm knob
Aperture diaphragm
knob
Press the power switch
to the "◯" position.

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