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6
Troubleshooting
If the microscope does not function property, take appropriate action as described below. If the
problem is still not resolved after referring to "Troubleshooting," please contact your nearest
Nikon representative
Optical
6.1
Problem
View field vignetting
Uneven illumination
across the view field
View field not visible
Dirt or dust in the field
of view
Poor image quality
Poor contrast
Poor resolution
Uneven focus
Possible causes
Parts installed incorrectly
Movable parts not switched correctly
Condenser adjusted in correctly
Improper objective-condenser
combination
Lamp installed incorrectly
Field diaphragm not sufficiently
opened
Dirt and dust on lens, condenser,
eyepiece lens, filter and specimen
Field diaphragm image not focused
on the specimen surface
Field diaphragm closed too far
Field diaphragm closed or opened
too far
Objective correction ring not
adjusted
Condenser maladjusted
Cover glass unattached
Cover glass thickness not as specified
Tip pf the oil-immersed objective
not immersed in oil
Unspecified immersion oil used
Air bubbles in immersion oil
Immersion oil attached to tip of the
dry objective
Dirt or dust on lens, condenser, eye
piece lens, filter and specimen
Revolving nosepiece not installed
correctly, or not rotated to the click
stop position
Specimen projecting from the stage
surface
64
Remedy
Install the Parts (nosepiece, condenser,
field diaphragm unit, aperture diaphragm
unit, etc.) correctly.
Switch the parts (optical path switching
lever, excitation method switching turret,
nosepiece, filter slider, condenser turret,
etc.) correctly. (Operate the parts to a
position until you feel resistance.)
Adjust the condenser so that the field
diaphragm image is shown in the center
of the viewing field.
Use a proper combination.
Install the lamp securely and adjust it as
necessary.
Open the field diaphragm to the proper
setting.
Clean.
Focus and center the condenser.
Open the field diaphragm to the proper
setting.
Open or close the field diaphragm to the
proper setting
Adjust the correction ring to match the
cover glass.
Adjust the condenser so that the field
diaphragm image is shown in the center
of the viewing field.
Attach a cover glass of the specified
thickness (0.17 mm).
Use Nikon immersion oil.
Remove the air bubbles.
Clean.
Clean.
Install correctly and rotate to the click
stop position.
Securely attach the specimen to the
stage specimen holder.
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