Nikon ECLIPSE 80i Instructions Manual page 23

Microscope
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Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)
12
Switch to the desired objective
and view the specimen.
Adjust the field diaphragm and
aperture diaphragm each time
you change objectives.
Field diaphragm:
Set slightly narrower than the margins of
the field of view.
Aperture diaphragm:
70% to 80% of the maximum numerical
aperture (N.A.) of the objective
13
Turn off power after completing
observations.
1.3 Bright-Field Microscopy
21
Select the
desired objective.
Aperture diaphragm
Field diaphragm knob
knob
1
Press the power
switch to the "◯"
position.

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