Table of Contents
Advertisement
Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)
10
Focus on the specimen.
11
Center the field diaphragm of the
Epi-illumination attachment.
Refer to "4. Epi-fluorescent Field Diaphragm" in
"17. Fluorescence Observation" of the separate
Reference manual.
12
Switch to the desired objective
and view the specimen.
• Use the ND filters for the fluorescence
attachment to adjust brightness.
• Adjust the field diaphragm so that it extends
slightly beyond the field of view.
• The image brightness can be adjusted with
the aperture diaphragm. Complete centering
before using the aperture diaphragm.
• When using oil immersion objectives, apply
immersion oil between the specimen and the
objective.
2.4 Epi-fluorescence Microscopy
33
Focus on the specimen using
the coarse and fine focus knobs.
Field diaphragm
open/close lever
Field diaphragm
centering screw holes
Field
diaphragm
image
Eyepiece
field of view
Select the desired
objective.
ND4 filter slider
ND8 filter slider
ND16 filter slider
2
Hide quick links:
Advertisement
Table of Contents
