Autoclaving; Autoclaving The Tubing - Gilson 402 User Manual

Maintenance

Autoclaving

Certain parts of the 402 can be autoclaved (20 min.
at 0.1 MPa, 121° C). They must be removed from
the instrument for autoclaving.
The autoclavable parts are:
• The valve (disassembled) or the Tee-junction.
• The syringe (except the 25 mL syringe).
• The piston assembly.
• PTFE and FEP tubing.

Autoclaving the Tubing

The tubing can be cleaned or autoclaved.
This procedure prevents the end of the tubing from
deforming. It is only necessary for the first time you
autoclave the tubing.
Precautions to take when autoclaving the tubing:
1) To preserve the flanged end of the tubing,
perform the following steps for the first time
that you autoclave these components.
2) Insert and tighten the flanged end of the inlet
tubing into one end of the PVDF union connector.
Tighten the flanged end of the outlet tubing to the
other end of the PVDF union connector. Connect
the other end of the inlet tubing to the filter.
3) Autoclave the tubing while connected to the
union connector.
4) Disconnect the PVDF union connector.
5) Autoclave the tubing a second time to sterilize
the interior of the tubing.
Transfer Tubing
tubing
connector
Union Seal
connector (threaded)
6
6-5