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Hydraulic Components; Valve; Tee-Junction; Syringes - Gilson 402 User Manual

Dilutor dispenser

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Description

Hydraulic Components

Valve

The two-position valve, which is fitted to the left-
hand side of the 402, has three ports. It switches
the syringe port to either the reservoir or to the
probe side (see opposite figure).
The valve, which has a 0.5 sec rotation period, is
driven by a stepper motor. It is designed to with-
stand a million rotations, when used with water.
The liquid contact surfaces of the valve, which is
autoclavable, are in PEEK and ceramic (121°C,
0.1 MPa, 20 min).
Tee Junction
The Tee-junction, which is fitted to the right-hand
side of the dual-syringe model, has three ports: the
inlet from the valve, the outlet to the probe, and the
syringe port (see opposite figure).
The Tee-junction, which has no internal moving
parts, includes 2 channels, each 1.5 mm in diam-
eter oriented at 120°. The liquid contact surfaces of
the Tee-junction, which is autoclavable, are in PEEK.

Syringes

The choice of syringe allows the 402 Dilutor-Dispenser
to handle volumes from 1 µL to 25 mL with low
values of systematic and random error. The 402
Dilutor-Dispenser is able to transfer liquid as
follows:
- from 1 µL to 10 mL from the probe (transfer
tubing volume of up to 10 mL),
- up to 25 mL from the reservoir, in one stroke,
- up to 99 mL from the reservoir, in several strokes.
When using the Dual-syringe model, a theoretical
dilution ratio of 1/25000 is achieved in one stroke
of both sample and diluent syringes. However, you
are advised not exceed a ratio of 1/500 for any
given dilution, in order to stay within the RSD
range (after efficient mixing).
Probe (or Tee
From reservoir
junction)
To waste
Syringe
From Valve
Syringe
2
To Probe
2-5

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