Gilson 402 User Manual

Dilutor dispenser

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402 Dilutor Dispenser

User's Guide

Table of Contents

Summary of Contents for Gilson 402

Page 1 402 Dilutor Dispenser User’s Guide...
Page 5: Table Of Contents
Table of Contents Safety 1 Introduction 2 Description 3 Installation Contents Notational Conventions ... 1-2 Overview ... 1-3 Unpacking ... 1-5 Warranty ... 1-6 Customer Service ... 1-7 Single-Syringe Model ... 2-2 Front Panel ... 2-2 Rear Panel ... 2-2 Dual-Syringe Model ...
Page 6 Fit the Syringe(s) ... 4-7 Creating a Method (Edit) ... 4-7 Prime the 402 (Man) ... 4-10 Running a Method (Run) ... 4-11 Correct Use of the 402 ... 5-2 Single-Syringe Model ... 5-2 Dual-Syringe Model ... 5-2 Software ... 5-5 Entering the Configuration (Conf)...
Page 7 Changing the 25 mL Piston Noses ... 6-11 Changing the 5 mL and 10 mL Piston Noses ... 6-11 402 Single Syringe ... Appendix A-2 402 Dual Syringe with Tee Junction Config..Appendix A-3 Additional Accessories ... Appendix A-4 Spare Parts ... Appendix A-5 Materials in Contact with Liquids ...
Page 9 Power supply cord reference 7080316106 is for use in France and Germany. Power supply cord reference 7080316105 is for use in USA and Canada. For other countries contact your local Gilson distributor. You must only use the type of fuse described in this document: 1.0 Amp type “T” slow blow.
Page 10 If a cloth dampened with soapy water is used to clean the instrument, only domestic soap may be used. No other form of detergent or chemical may be used. These electronic and hazard symbols appear on the 402 Dilutor Dispenser:...
Page 11 Introduction The following chapter describes the instrument, the service and warranty conditions.
Page 12: Notational Conventions
The conventions in this User’s Guide are as follows. Used in the stand-alone mode, this instrument is referred to as ‘402 Dilutor-Dispenser’ or simply 402. Specific keys to press on the Gilson Keypad are represented in bold capitals, for example: ‘key in the value and press ENTER’;...
Page 13: Overview
Refer to Appendix A for lists of the standard equipment, additional accessories and spare parts. The 402 DD is supplied with a tubing kit for con- necting to a solvent reservoir. Syringes are not included in the standard equipment; you should order syringe(s) appropriate to your Method (volume, systematic error and flow rate requirements;...
Page 14 Introduction Good Laboratory Practice (GLP) is facilitated by the following: codes to protect the program files against unau- thorized modification, counters for the number of syringe and valve operating-cycles, checking protocol for the hydraulic specifica- tions (IQ/OQ procedures), correction of the syringe-piston stroke to com- pensate for environmental factors (temperature, viscosity, etc.).
Page 15: Unpacking
You should check off the parts received in the box against the parts list (refer to Appendix A), at the time that you receive the shipment, even if the 402 is not to be used immediately. Your Gilson distribu- tor should be notified immediately of any inconsist-...
Page 16: Warranty
Introduction Warranty If the 402 Dilutor-Dispenser does not appear to function correctly, first verify the electrical connec- tions are correct and that the instrument is switched ON. Contact your Gilson distributor for technical advice or possibly a service visit. Any service required will be given within the warranty conditions assured by your Gilson distributor.
Page 17: Customer Service
Introduction Customer Service Gilson and its worldwide network of authorized distributors provide you with four types of assist- ance: sales, technical, applications and service. Customer Service personnel are able to serve you more efficiently if you provide the following information: •...
Page 18 Introduction...
Page 19 2-6, most suited to your application. Your 402 is delivered with the appropriate modules fitted. If later, you decide to convert a Single-syringe model into a Dual-syringe model, you must order and fit a Tee-junction module to the right-hand side of your dilutor;...
Page 20: Single-Syringe Model
The rear panel holds a socket for connecting the keypad, a connector for the probe, the ON/OFF switch, and voltage selector/fuse holder. There is also a Gilson Serial Input Output Channel (GSIOC) connector, which is a communications channel to link the 402 Dilutor-Dispenser with other equipment, if required.
Page 21: Dual-Syringe Model
Description Dual-syringe Model The Dual-syringe model is fitted with a Valve module at the left and a Tee-junction module at the right. The modules are linked by plastic tubing. Bottle Clip for Tubing Tubing Clip (Self-adhesive) Cable Support Valve Module ON/OFF indicator Clamp for Probe Front Panel...
Page 22: Keypad And Probe
The assembled probe fitted with the standard probe nozzle is shown opposite. Assembly and installation of the standard probe is described in Chapter 3. A kit is available to enable you to fit Gilson’s Diamond® tips (D200, D1000) to the probe (see Chapter 3).
Page 23: Hydraulic Components
Hydraulic Components Valve The two-position valve, which is fitted to the left- hand side of the 402, has three ports. It switches the syringe port to either the reservoir or to the probe side (see opposite figure). The valve, which has a 0.5 sec rotation period, is driven by a stepper motor.
Page 24 0.5 mL 5 mL 10 mL There are seven syringe sizes available for the 402, all except the 25 mL are autoclavable. The lifetime of all pistons (see Chapter 6), when they are used with water at a pressure of up to 0.3 MPa is over 30000 cycles (each cycle contains 2 strokes: one up and one down).
Page 25 Installation This chapter explains how to install the fuses, syringes and hydraulics according to your system requirements. This chapter explains how to add the extra module to upgrade your 402 to a dual syringe pump.
Page 26: Assembling The Probe
8. Connect the other end of the tubing to the outlet of the Valve (Single-syringe) or the Tee-junction (Dual-syringe). 9. Use the 402 Software to check (and change, if necessary) the configuration using the Conf (Vol) menu. Adapter Kit for Disposable Tips...
Page 27 4. Plug the end of the control cable into the socket, labeled ‘PROBE’ at the back of the 402. 5. Fit the clamp at the right-hand side of the 402 (see on the previous page). 6. Mount the probe on the clamp.
Page 28: Connecting The Tubing
Single-syringe Model Valve Inlet Tubing A length of Fluoride Ethylene Propylene (FEP) tubing, supplied with the 402, is used to connect the inlet port to the solvent reservoir. Screw the connector into the inlet port (left-hand) of the valve and put the other end into the reservoir. A bottle clip is provided to hold the tubing securely in the reservoir (see opposite Figure).
Page 29: Dual-Syringe Model
Valve Inlet Tubing A length of Fluoride Ethylene Propyl- ene (FEP) tubing, supplied with the 402, is used to connect the inlet port to the solvent reservoir. Screw the connector into the inlet port (left- hand) of the valve and put the other end into the reservoir.
Page 30 1 mL, 5 mL, and 10 mL are available as additional accessories. Self-adhesive clips are provided for holding the transfer tubing securely against the side of the 402 (see previous page). The 1 mL transfer tubing is recommended for transferring volumes smaller than 1 mL. To avoid...
Page 31: Electrical Connections
Installation Electrical Connections The rear panel of the 402 Dilutor-Dispenser houses: - sector block containing the power socket, fuse drawer and ON/OFF switch, - keypad connector, - probe connector, - GSIOC connector. Sector Block The sector block contains the power socket, fuse holder and ON/OFF switch.
Page 32: Connecting The Power Cord
402 (as in Chapter 2), or even at some distance away from it. However, you can also install the keypad on top of the 402; to do this you should insert the tab on the rear support of the keypad into the slot on top of the 402 and then gently push the tab in as far as it will go.
Page 33: Syringes
Syringes The 402 Dilutor-Dispenser is supplied without syringes. Order a syringe (or syringes), appropriate to your Method, for your 402 Dilutor-Dispenser. Mount the syringe on the valve or Tee-junction. Assembling a Syringe The syringe and its piston are supplied assembled.
Page 34: Upgrading To Dual-Syringe Model
Installation 3-10 Upgrading to Dual-syringe Model The single syringe 402 can be upgraded to improve the versatility of the system. To upgrade to the Dual-syringe 402, you must install a Tee-junction module (kit reference F4015010) at the right-hand-side of the 402. You must first install the syringe module (includes motor etc.) and...
Page 35: Removing The Cover
Removing the Cover 1) Undo the four screws (with two washers) at the base of the 402 that secure the top cover. Place the 402 in the upright position, pull the sides of the cover outwards, and then lift off the cover.
Page 36: Inserting The Module
4) Replace the cover by pulling the sides outwards slightly and lowering the it over the 402. Insert and tighten the four screws at the base of the 402 that secure the cover to the lower chassis. Do not forget to reinstall the washers, which are designed to ensure that the cover and chassis are con- nected electrically.
Page 37: Operation - Standard Use
This chapter describes how to operate the 402 in standard use. Chapter 5 contains more detailed and advanced information, together with less frequently used options. You are advised to read the first section of Chapter 5, ‘Correct Use of the 402’, before proceeding.
Page 38: Essential Steps
Assemble and Connect the Probe Assemble and connect the probe (see Chapter 3). Connect the Tubing If you have a Single-syringe 402, install the tubings as described in Chapter 3, page 3-4. You must con- nect 3 pieces of tubing: inlet (reservoir), transfer...
Page 39: Switch-On
4 pieces of tubing: inlet (reservoir), Valve to Tee-junction, transfer (probe), and waste. Switch-on 1) Switch on your 402 (the power switch is on the back panel): the indicator light on the front panel comes on, the keypad is illuminated, and briefly displays messages giving the software version numbers, Initialization Screen 1, for example.
Page 40: Configuration (Conf)
Conf soft-key (see Configuration Screen). As a minimum, you must enter the volume of syringe(s) that you are going to fit to your 402, check that the default flow rates are appropriate (or enter suitable values), and check the transfer (probe) tubing volume is correctly specified (standard is 2 mL).
Page 41 Flow Rates Flow rates should be specified according to the sizes of syringe and tubing fitted to the 402. Refer to Chapter 5 for more details, the maximum values are given in the Tables, these are used as default values by the software.
Page 42 (for example) aspirating air via the probe. This feature enables you to work faster with the 402. To switch between ON and OFF, press the Chge soft-key, then press ENTER to confirm. Press Prev to go to the Beeper Screen.
Page 43: Fit The Syringe(S)
Operation - Standard Use Fit the Syringe(s) 1) First you must lower the piston rod(s), as follows: - press the Conf key to access the Configuration Screen: - press the Syr soft-key (ensure that the size is correct before continuing), - press the Exch soft-key.
Page 44 Operation - Standard Use Start-up Screen Task Selection Screen In-run Prompts You can defer some parameters (volumes and flow rates) by entering a question mark (?) in place of the value. You enter the actual value after pressing the RUN key. This feature is particularly useful when optimizing parameters.
Page 45 Press Yes; Run Screen appears (the name of the first task to be run appears, in this example ‘Dispense’); you are now ready to prime the 402, after which you can run the Method that you have just created. Dispense Screen (1)
Page 46: Prime The 402 (Man)
Press Prime, Initialization Screen 2 appears. [Measure and Titrate are described in Chapter 5.] You should prime the 402 before each run, to ensure that all fluid pathways contain fresh solvent and no air bubbles. Put the probe-end of the transfer-tubing into a suitable the waste container, then press OK.
Page 47: Running A Method (Run)
Operation - Standard Use ‘Homing ...’ appears on the screen until the syringe(s) have returned to their uppermost (home) positions, during which time any liquid contained in the transfer-tubing is expelled from the probe-end. After this the Action Screen reappears. Press ESC to return to the start-up screen for the current file.
Page 48 Operation - Standard Use In Run Screen (1) In Run Screen (2a) In Run Screen (2b) 4-12 Ensure that the probe-end of the transfer-tubing is in a suitable waste container, then press OK. ‘Homing ...’ appears on the screen until the syringe(s) have returned to their home positions, during which time any liquid contained in the transfer-tubing is expelled from the probe-end.
Page 49 Operation - Standard Use (ii)Press the command button to aspirate AIR. The command light is steady and you see alternately In Run Screens 3a and 3b. (iii) Put the tip of the transfer-tubing into the sample then press the command button to aspirate liquid.
Page 50 Press ‘Abort’ to confirm that you wish to stop and you are returned to the Run Screen. Alternatively, you can bring the 402 to a halt by switching off the power, in which case you must ensure that the probe end of transfer-tubing is in a suitable...
Page 51: Operation - Advanced Use
Operation - Advanced Use This chapter explains in details how liquids are aspirated and dispensed using the 402 Dilutor-Dispenser and gives in depth details of various software functions.
Page 52: Correct Use Of The 402
Operation - Advanced Use (a) Aspirating from Reservoir From Reservoir Piston descends to aspirate Correct Use of a 402 Single-syringe Model • Samples and reagents are aspirated from the probe side. • Liquid aspirated from the probe side may not enter the syringes.
Page 53 Operation - Advanced Use • If a drop of liquid is present at the end of the tubing after dispensing, you must include this drop in the result vial. • The larger capacity syringe must always be mounted on the left-hand side. •...
Page 54 Operation - Advanced Use (c) Left-hand Syringe Aspirating from Probe From Tee Left piston descends to aspirate (d) Right-hand Syringe Aspirating from Probe Valve switched to Reservoir Left piston Right piston remains descends to inactive aspirate (e) Right-hand Syringe Dispensing to Probe Valve switched to Reservoir Left piston...
Page 55: Software
(ii) Run a prefabricated Method (files 90 - 94). (iii) Create your own Method. A Method consists of a file saved in the 402’s memory, which can hold up to 99 files. Each file will contain one or more of the following tasks: Dilute (Dil), Dispense (Disp), Pipette (Pip), Rinse, and Mix.
Page 56: Entering The Configuration (Conf)
8 Press Deflt to select the default value or option. Entering the Configuration (CONF) You have to tell the controlling software how your 402 Dilutor-Dispenser is configured (syringe sizes, flow rates, etc.). Press the CONF key; you see the Configuration Screen.
Page 57 Operation - Advanced Use If you want to change the configured volume, press Chge (until you reach the correct value); when the volumes are the same press ENTER, press the ESC key to return to the Configuration Screen, or press Next, after which you see the Air Gap Volume Screen.
Page 58 Operation - Advanced Use Syringe Correction Screen When you turn the syringe correction factor ON, a plus sign (+) appears after the file number, as a reminder. Air Flow Screen Resv Flow Screen Prime Flow Screen When you specify left and right syringe capacities (left must be >...
Page 59: Creating A Method (Edit)
Under normal use you should expect to fit new (or refurbished) syringes after 30000 cycles with water. This feature is made easy by the 402, which automati- cally counts the number of operating cycles performed by the syringe(s) and valve. (A cycle is completed when the syringe leaves its home position.) You...
Page 60: Saving A Method
Operation - Advanced Use Syringe Correction Screen Save Screen File Menu Screen File Lock Screen Delete Screen 5-10 to access the required task and its parameters, (ii) if you have entered a syringe correction factor in the configuration process, the Syringe Correction Screen appears first, after which you select ON or OFF using the Chge soft-key, press ENTER, and then Next, after which the Task Selection...
Page 61 Operation - Advanced Use When you select Yes, the file is deleted from the directory and you see the New-File Directory Screen. Press ESC to return to the New File Screen, or press Dir to access the file directory. Copy a File This action applies to the file currently selected;...
Page 62: Direct Manipulations (Man)
Press the Man soft-key, the Action Screen appears. Prime You should prime the 402 before each run, to ensure that all fluid pathways contain fresh solvent. Press Prime, Initialization Screen 2 appears. Put the probe-end of the transfer-tubing into a suitable waste container, then press OK.
Page 63 You can measure a volume (e.g. supernatant after centrifugation). • Press Measure, the Measure Screen appears. For a Dual-syringe 402, press the Chge soft-key to select LEFT or RIGHT. You are advised to select the left-hand syringe if the volume to be measured seems to be significantly larger than the capacity of the right-hand syringe.
Page 64 With 402, you may also use titrate to fill plates, vials, or bottles. To titrate, you aspirate a known volume of reagent,...
Page 65: Tasks - Detailed Descriptions
Operation - Advanced Use This is realized using the 402 by pressing the probe’s command button with increasing rapidity as you approach the neutralization point. Care must be taken in selecting the dispense flow rate. • Press Titrate, Titrate Screen (1) appears. Select...
Page 66 ‘Parameters Incompatible!’ on the screen. At this point you can either press Quit and reconfigure the 402 or press OK and renter one or more of the volumes entered in the task. When you configure your 402 for use with dispos- able tips, the ‘Extra volume’...
Page 67 Operation - Advanced Use Parameter Air-gap Volume (in Configuration) Dilution Ratio (Dual-syringe) Dilution Ratio (Single-syringe) Number of Serial Dilutions Number of Reagents Number of Fractions Number of Cycles (mixing) Sample Volume (Vs) Reagent Volume (Vr) Diluent Volume (reservoir) Diluent Volume (via probe) Common Volume (reservoir) Common Volume (via probe) Total Volume (/)
Page 68 Operation - Advanced Use If you specify a volume of sample greater than that of the right- hand syringe, the left-hand will be used to aspirate the sample. diluent result air gap sample Screens: 5-18 Dilute Three types of dilution task are available ‘Normal’, ‘Unknown’, and ‘Serial’.
Page 69 Operation - Advanced Use Press Next, then key in the sample volume, press ENTER. Depending on the mode, you see one of three screens after pressing Next. Key in the value, press ENTER. Optionally, for any mode, you can display the variables calculated by the software by pressing Data.
Page 70 Operation - Advanced Use Screens: 5-20 For each dilution in the series: Vt = Vs x Rd, Vd = Vt - Vs For example, after selecting Dilution Ratio Mode, you enter Sample Volume = 10 and Dilution Ratio = 3; the software calculates the Total Volume as 30 and the required Diluent Volume as 20.
Page 71 Operation - Advanced Use Optionally, for any mode, you can display the variables calculated by the software by pressing Data. Optionally, you can modify the flow rates; press Flow, For any mode, key in a value for Asp flow, press ENTER, press Next;...
Page 72 Yes, the Method is then saved, after which you see the Run Screen. Run Procedure Press Run Select the syringe (for the Dual-syringe 402 only), press the Chge soft-key to select LEFT or RIGHT, press ENTER to confirm. You are advised to select the left-hand syringe if the volume to be measured seems to be significantly larger than the volume of the right-hand syringe.
Page 73 Operation - Advanced Use If for any reason you want to repeat the measure- ment (e.g. you need to select the LEFT syringe instead of the RIGHT), put tip of the transfer- tubing back into the original container, then press Redo to discharge the liquid.
Page 74 Operation - Advanced Use 5-24 volume of liquid, is dispensed, at the end of the process, into the waste. When you configure disposable tips in air mode, ‘Extra volume’ is replaced by ‘Air push volume’ in this task. 1. Aspirate an air gap. 2.
Page 75 Operation - Advanced Use Press ESC (twice). Press ESC. Pipette You use this task to aspirate a series of fractions via the probe, then dispense the total volume aspirated into a recipient vessel. Each liquid aspirated is sepa- rated by an air gap (if air gap is configured as > 0). You may also aspirate an ‘Extra volume’...
Page 76 Operation - Advanced Use 5-26 Optionally at the first of the volume screens, press Flow. Optionally at the first of the volume screens, press Extra, Key in ‘Extra volume’, press ENTER. Rinse The purpose of this task is to minimize carry-over by flushing the transfer-tubing and syringes with solvent from the reservoir, between tasks.
Page 77 Operation - Advanced Use Press Mix, then select the mixing mode (air or asp/ disp). Press Chge to switch modes. Press ENTER, then Next. Key in volume, press ENTER. Optionally, press Flow. • For AIR mode, key in a value for Disp flow, press ENTER.
Page 78 File # (44) ENTER Prefabricated Methods Five prefabricated Methods, to run or to adapt, are present in the 402’s memory as file numbers 90 to 94. Although the tasks are the same, the parameters vary according to whether you have a Single- syringe or a Dual-syringe 402.
Page 79 Operation - Advanced Use File Number TASK/Parameter Method 92 Dispense Sample Source Fractions Nb of Fractions Common Volume Method 93 Dispense Sample Source Fractions Nb of Fractions Fraction 1 Fraction 2 Fraction 3 Adjust Source Final Volume Method 94 Pipette Fractions Nb of Fractions Fraction 1...
Page 80 When you have finished, or wish to change programs, press STOP, to instantly halt the program. Alterna- tively, you can bring the 402 to a halt by switching off the power, in which case you must ensure that the probe end of transfer-tubing is in a suitable waste container before switching on again.
Page 81: Additional Information
Operation - Advanced Use Additional Information Flow Rates Flow rates depend on the nature of the liquid (viscosity), the capacity of the syringe, and the internal diameter of tubing. For liquids transferred via the probe, it is possible to adjust the aspirate and dispense flow rates according to the nature of the liquid (e.g.
Page 82 Operation - Advanced Use Maximum Dispense Flow Rate (No Air Gaps) Tubing Type 1 mL 2 mL 5 mL 10 mL Micro Tips (liquid) Micro Tips (air) Large Tips (air) Recommended Flow Rate with an Air Gap (10 µL) Syringe size Flow rate (mL/min) 100 µL...
Page 83: Syringe Correction Function
A correction factor may be applied to all volumes of liquid to compensate for the effects of temperature (for example, when the 402 is used in a culture room) or for the effects of viscosity. This factor should be calculated from gravimetric assays and entered in the Configuration menu.
Page 84 100 µL). The liquid is ejected with an Air push volume of at least 20 µL when using a Gilson D200 tip and 100 µL when using a Gilson D1000 tip. An automatic software correction of 2% of the piston stroke is made to improve accuracy with Gilson D200 and D1000 tips.
Page 85: Maintenance
Maintenance This chapter explains how to perform the routine maintenance procedures. The only maintenance operations necessary for the 402 DD are: • Cleaning the exterior of the instrument. • Cleaning of the valve, Tee, syringe assemblies and the tubings. • Replacement of the piston syringe after approximately 30 000 cycles.
Page 86: Cleaning
Parts in the liquid flow path may be cleaned with ethanol or bleach (10 % Sodium Hypochlorite). Cleaning the Valve The valve must be cleaned after using the 402 with corrosive or hazardous liquids, if leakage has occurred, or if liquid flows back into the waste tubing, or if the dilutor has been left unused for a while after pumping alcohol based liquids.
Page 87: Cleaning The Tee Junction
2) Dismount the Tee junction and syringe assembly and separate the syringe from the Tee junction. 3) Undo the two screws securing the Tee junction to the 402 front plate and remove the Tee junc- tion. 4) Autoclave the Tee junction.
Page 88: Cleaning The Syringe Assembly
2) Undo the piston securing screw to free the piston assembly from the piston operating rod. 3) Lower the piston operating rod; for Gilson soft-ware there is a change syringe program that lowers the piston operating rod - use this software option to lower the piston operating rod away from the syringe piston.
Page 89: Autoclaving
Maintenance Autoclaving Certain parts of the 402 can be autoclaved (20 min. at 0.1 MPa, 121° C). They must be removed from the instrument for autoclaving. The autoclavable parts are: • The valve (disassembled) or the Tee-junction. • The syringe (except the 25 mL syringe).
Page 90: Changing The Syringe Piston Assembly
Maintenance Item Part Number 100 µL piston kit F4015072 250 µL piston kit F4015073 500 µL piston kit F4015074 1 mL piston kit F4015075 5 mL piston kit F4015076 10 mL piston kit F4015077 25 mL piston kit F4015078 Changing the Syringe Piston Assembly For all syringe sizes, it is necessary to replace the piston assembly after approximately 30 000 cycles...
Page 91: Changing The Piston Syringes And Piston Noses
Maintenance Changing the Piston Syringes and Piston Noses The piston nose should be replaced after the pump has performed approximately 30 000 cycles. All piston noses are replaceable, the process is relatively easy, but for most pistons (except the 25 mL pistons), special tools are required to mount the piston nose.
Page 92 Maintenance Piston rod Piston button Allen screw Piston nose Nose holding tool Allen screw Piston button Piston rod Support collar Piston nose Syringe barrel Valve screw 1. Remove the syringe from the pump as described in Chapter 4. 2. Slide the piston out of the syringe barrel. 3.
Page 93: Changing The 500 Μl Piston Noses
Maintenance Changing the 500 µL Piston Noses 1. Remove the syringe from the pump as described page 6-4. 2. Slide the piston out of the syringe barrel. 3. Remove the piston nose from the piston rod by carefully pinching the piston nose with a pair of cutters and gently pulling the piston nose off the piston rod.
Page 94: Changing The 1 Ml Piston Noses
Maintenance Cover seal Valve screw Syringe body Part Number Description F4015065 5 x 1 mL piston noses and mounting tool. F4015005 1 nose mounting tool. Piston rod Piston nose Piston nose holding tool 6-10 Before Mounting a 500 µL Syringe: To optimize the fitting of the 500 µL syringe on the Dilutor valve, the 500 µL syringe is supplied with a cover seal.
Page 95: Changing The 25 Ml Piston Noses
Maintenance Changing the 25 mL Piston Noses You don’t need a special tool for mounting the piston nose on the 25 mL piston. The nose screw- fits onto the piston shaft. 1. Remove the syringe from the pump as described page 6-4.
Page 96 Maintenance Piston Piston nose holder O-ring nose 6-12 5. Insert the new piston nose in the recess of the piston nose holder. Make sure that the aperture is facing outward and the O-ring is inside the seal. 6. Place the piston shaft in the piston shaft holder. 7.
Page 97 Troubleshooting This chapter explains how to diagnose possible problems and how to overcome them.
Page 98: Mechanical Problems
Troubleshooting Problem Valve does not fit on to the 402. Valve moves during aspirate or Syringe does not move properly. Tubing blocked. Valve sticks Mechanical Problems Possible Cause Valve axle not properly aligned with the motor drive. Valve mounting screw not tight.
Page 99: Hydraulic Problems
Troubleshooting Hydraulic Problems Problem Possible Cause Liquid leakage Connections incorrectly made. Liquid leaving by the Tubing blocked. waste tube. Dirt trapped between the ceramic surfaces. Solution Tighten all tubing connections. Check all tubing for blockages. Ensure that the inlet filter is clear. Clean the valve.
Page 100: Electrical Problems
Troubleshooting Problem Instrument does not respond. Electrical Problems Possible Cause Power cord not connected. 402 is not switched on. Fuse blown. Solution Check power cord is properly connected. Check ON/OFF switch. Check the fuses. See page 3-7.
Page 101 Parts & Accessories Lists Appendix Parts lists for the 402 Dilutor Dispenser, consisting of Standard Equipment, Additional Accessories, and Spare parts. Appendix A-1...
Page 102: 402 Single Syringe
Power cord 100-115 V 6730104006 1.0 Amp type T slow-blow fuses 54080181 Clips for tubing, self-adhesive LT801310 402 Dilutor Dispenser User’s Guide LT801311 402 Dilutor Dispenser Quick Guide cards LT804021 IQ Procedure LT804035 Quality Control Certificate LT804046 OQ Procedure Appendix A-2...
Page 103: Dual Syringe With Tee Junction Config
Parts & Accessories Lists 402 Dual Syringe with Tee Junction Configuration Part Number Qty Description F410515 402 Dilutor Dispenser (Dual-syringe) F4015016 Probe (standard) F40151403 Probe support 4011534125 Fixing screw for probe support 130221 Keypad F4015032 Tubing, tapered transfer (probe), FEP (2 mL, 1300 x 1.5 mm id)
Page 104: Additional Accessories
Parts & Accessories Lists Part Number Description F4015015 Adapter kit for disposable tips 25025341 100 µL syringe 25025342 250 µL syringe 25025347 500 µL syringe 25025343 1 mL syringe 25025344 5 mL syringe 25025345 10 mL syringe 25025346 25 mL syringe F4015031 Tubing, tapered transfer (probe), FEP (1 mL), (1 x 2 x 1300 mm) F4015033...
Page 105: Spare Parts
Parts & Accessories Lists Spare Parts Part Number Description F4015072 100 µL piston kit F4015073 250 µL piston kit F4015074 500 µL piston kit F4015075 1 mL piston kit F4015076 5 mL piston kit F4015077 10 mL piston kit F4015078 25 mL piston kit Appendix Appendix A-5...
Page 106 Parts & Accessories Lists Appendix Appendix A-6...
Page 107: B - Chemical Compatibility
Chemical Compatibility Appendix This chapter contains information about the chemical compatibility of the syringes and the tubing. Appendix B-1...
Page 108: Materials In Contact With Liquids
Chemical Compatibility Appendix B-2 Chemical Compatibility Materials in Contact with Liquids In the 402 Dilutor Dispenser flow-path, several materials are in contact with liquids: Inlet tubing, Transfer tubing PTFE/Ekonol Piston nose of the syringes (except 25 mL) PTFE Piston nose of the 25 mL syringe...
Page 109: C - Technical Data
Technical Data Appendix This chapter contains the Technical Data concerning the 402 Dilutor Dispenser. Appendix C-1...
Page 110: Hydraulics
Technical Data Appendix C-2 Hydraulics Dilution Ratio When using the dual syringe model equipped with a 25 mL syringe on the left and a 100 µL syringe on the right, the dilution ratio is 1/25000, but in order to stay within the RSD range a maximum ratio of 1/500 is recommended.
Page 111 Technical Data Syringes Syringe capacity (µL) Maximum Flow (mL/min) Minimum Specified Volume (µL) Nominal Volume (µL) Performance Specifications The following values are obtained using a defined protocol. If this protocol is followed, the obtained values must be inferior or equal to the listed figures. Syringe Volume Systematic error Random error capacity (µL) pipetted (µL) %...
Page 112 Technical Data For 1 % syringe capacity, typical values are given. Appendix C-4 The same specifications are obtained in the dispensing mode with liquid from the reservoir. See OQ Document for procedure and calculations. Syringe Volume capacity (µL) pipetted (µL) % 1000 5000 10000...
Page 113: Mechanics
Technical Data Appendix Mechanics Valve Type: Three port, two position. Angle between ports: 120°. Valve drive: Stepper motor. Rotation time: 0.5 Second. Liquid contact materials: PEEK and ceramic. Lifetime: 1 000 000 cycles. Dead volume: 123 µL. Maximum Pressure: 0.8 MPa. Tee Junction Type: Three port, no moving parts.
Page 114: Environmental
Technical Data Appendix C-6 Environmental Conformity to Norms Refer to separate leaflet LT801354 ‘Declaration of Conformity’. Storage Indoor use only. Installation: Category II. Altitude: operating storage Temperature range: operating storage Pollution degree 2. Humidity: Maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.
Page 115 Error Messages Appendix This chapter contains the Error Messages concerning the 402 Dilutor Dispenser. Appendix D-1...
Page 116: Error Messages
Error Messages Error Message Adjust volume too small Asp flow missing Diluent volume missing Disp flow missing DISP task in wrong place Incorrect flow rates Left syr life exhausted! LINK method not allowed! Max sample vol. reached Mechanical problem: Help Method does not exist! Method used in link file Method xxx is locked...
Page 117 Error Messages Error Message Parameters incompatible! Ratio impossible Ratio missing Reservoir not allowed Right syr life exhausted! Sample too small Sample volume missing Syr capacity too small Syringe Life Exhausted! Total volume is missing! Total volume missing Total volume too small Valve life exhausted! xxx exists, overwrite? Cause/Possible Action...
Page 118 Error Messages Appendix Appendix D-4...
Page 119 2-2, 2-3, 3-4, 3-5 Configuration Conformity to Norms Connecting cable keypad power cord probe tubing Conventions notational typographical Copy Correct use of the 402 Correction Factor Counters Cover cleaning removing replacing 4-10, 4-11 Creating a Method Customer Service Default values 4-10 Diluent...
Page 120 Index Installation Keypad Large Volumes Liquid contact materials Link Lock Maintenance Autoclaving Changing the piston assembly Cleaning the instrument Cleaning the syringe assembly Cleaning the tubing Cleaning the valve Disassembling the valve Dismounting the valve Measure Mechanical problems syringes tee-junction valve Method Example Method...
Page 121 Index Titrate Troubleshooting electrical problems hydraulic problems mechanical problems Tubing connecting transfer valve inlet valve waste Typical Values Union connector Unlock Unpacking Upgrading Valve disassembly dismounting inlet tubing connections outlet connections Viscous liquids Voltage range selection Volumetric analyses Warranty 5-14 5-11 3-10 5-33...
Page 122 Index Index-4...
Page 124 World Wide Web: www.gilson.com E-mail: sales@gilson.com, service@gilson.com, training@gilson.com World Headquarters Gilson, Inc. 3000 W. Beltline Hwy., P.O. Box 620027, Middleton, WI 53562-0027, USA Telephone: (1) 800-445-7661 or (1) 608-836-1551 • Fax: (1) 608-831-4451 Gilson SAS 19 avenue des Entrepreneurs, 95400 Villiers-le-Bel, France...