Gilson 402 User Manual page 67

Operation - Advanced Use
Parameter
Air-gap Volume (in Configuration)
Dilution Ratio (Dual-syringe)
Dilution Ratio (Single-syringe)
Number of Serial Dilutions
Number of Reagents
Number of Fractions
Number of Cycles (mixing)
Sample Volume (Vs)
Reagent Volume (Vr)
Diluent Volume (reservoir)
Diluent Volume (via probe)
Common Volume (reservoir)
Common Volume (via probe)
Total Volume (/)
Total Volume (via probe)
Extra Volume
Final Volume (adjust via probe)
Final Volume (adjust from reservoir)
Flow Rate - Aspirate (mL/min.)
Flow rate - Dispense (mL/min.)
Chaining Tasks
Because of the flexibility of the software many
combinations of the tasks are possible in your Method.
However, it is better to avoid certain combinations
of task such as Dispense followed by Dilution.
The reason is that Dispense distributes the fractions
into several tubes, whereas Dilute, Mix, and Rinse
operate on a tube by tube basis.
So, if Dilute follows Dispense, the software will first
dispense the number of fractions specified (into a
series of tubes), then one tube will be diluted, and
then the software will start the second dispense cycle.
When editing a Method containing (for example)
Dispense followed by Dilution, the software issues a
warning message indicating that it would be better
if Dispense could be placed at the end of the chain.
Minimum Maximum
0 Transfer-tubing Volume
1 100000
1 500
1 12
0 12
1 12 if volumes are different, 99 if the same.
0 12
0.1 Volume of transfer-tubing - air gap(s)
0.1 Volume of transfer-tubing - air gap(s)
0.1 99.999 mL
0.1 Volume of transfer-tubing - air gap(s)
0.1 99.999 mL
0.1 Volume of transfer-tubing - air gap(s)
0.1 99.999 mL
0.1 Volume of transfer-tubing - air gap(s)
0.1 100 µL
0.1 Volume of transfer-tubing - air gap(s)
0.1 99.999 mL
0.01 See page 5-31
0.01 See page 5-32
5
5-17