Syringe Correction Function; Use Of Disposable Tips - Gilson 402 User Manual

5
Operation - Advanced Use

Syringe Correction Function

A correction factor may be applied to all volumes of
liquid to compensate for the effects of temperature
(for example, when the 402 is used in a culture room)
or for the effects of viscosity. This factor should be
calculated from gravimetric assays and entered in the
Configuration menu. With the Dual-syringe model,
you may apply different corrections to each syringe.
When the syringe correction is switched on, a plus
sign (+) appears after the Method number. All
volumes in all the tasks are affected by this correc-
tion, when it is switched on. At the start of a task
edit, a message appears to inform you of the status
(Syringe Correction Screen, see page 5-8).
A correction factor of 1% (for example) under the
same condition of temperature and pressure in-
creases the volume of liquid aspirated or dispensed
by this amount.
The correction allows you to increase the volume
transferred, for example: 250 µL syringe, nominal
value is 100 µL, the actual value (after gravimetric
measurements) is 99 µL. In this case, a 1% correction
results in a nominal value of 101 µL, and an actual
value of 100 µL. The application of a 1 % correction
factor effectively reduces the capacity of the syringe
by 1 %.
You should follow the procedure for gravimetric
analysis described in the Operational Qualification
(OQ) document that accompanies this User's Guide.
Check the accuracy and RSD of the volumes with
the liquid to be transferred under the conditions of
pressure and temperature of the working environment.

Use of Disposable Tips

Using the disposable tip kit, Gilson D200 tips or
Gilson D1000 tips can be mounted at the end of the
probe. Two modes are available: air and liquid.
Air Mode
The use of 402 in 'Air Mode' is, for those users
familiar with Gilson's pipettes, the same as that of
'Pipetman'.
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