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Qiagen BioRobot M48 User Manual

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User Manual
January 2003

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Summary of Contents for Qiagen BioRobot M48

  • Page 1 User Manual January 2003...
  • Page 2 Trademarks Patented or patent-pending technology and/or registered or registration-pending trademarks of the QIAGEN Group: QIAGEN ® QIAsoft ™ , BioRobot ® , MagAttract ® Adobe is a registered trademark of Adobe Systems Incorporated. Celeron and Pentium are registered trademarks of Intel Corporation.

  • Page 3: Table Of Contents

    Introduction ..............2-1 About this manual.............2-1 General information..........2-2 2.2.1 Policy statement ..........2-2 2.2.2 Software for operating the BioRobot M48 ..2-2 2.2.3 Version management ........2-2 Intended use of the BioRobot M48......2-3 2.3.1 Requirements for BioRobot M48 users....2-4 BioRobot M48 — General Description .......3-1 External features of the BioRobot M48 ......3-2...
  • Page 4 AC power connection..........4-2 Hardware installation..........4-4 Software installation..........4-6 General Operation............5-1 Overview ..............5-1 Starting up and shutting down the BioRobot M48..5-2 5.2.1 Starting up the BioRobot M48......5-2 5.2.2 Opening the QIAsoft M software ...... 5-3 5.2.3 Closing the QIAsoft M software ......
  • Page 5 5.4.11 Checking the status of the protocol run....5-23 5.4.12 Viewing the results of the protocol run .....5-25 5.4.13 Sterilizing the BioRobot M48 ......5-27 Preventive Maintenance ..........6-1 Daily maintenance procedure ........6-2 6.1.1 Cleaning the tip guard insert......6-4 Weekly maintenance procedure .........6-4 6.2.1...
  • Page 6 Contents Appendix C............... C-1 Warranty statement ..............C-1 Liability clause ............... C-2 Index ................Index-1 Contents-4 QIAGEN BioRobot M48 01/2003...

  • Page 7: Safety Information

    The advice given in this manual is intended to supplement, not supersede, the normal safety requirements prevailing in the user’s country. QIAGEN BioRobot M48 01/2003...

  • Page 8: Proper Use

    Instrument damage caused by water or chemical spillage will void your Warranty. In case of an emergency, switch off the BioRobot M48 at the power switch on the left side of the instrument and unplug the power cord from the wall power outlet.

  • Page 9: Electrical Safety

    Safety Information Electrical safety To ensure satisfactory and safe operation of the BioRobot M48, it is essential that the line power cord is connected to true electrical earth (ground). WARNING Electrical hazard [W2] Any interruption of the protective conductor (earth/ground...

  • Page 10: Environment

    It has been subjected to severe transport stresses WARNING Risk of electric shock [W3] The panel on the left side of the BioRobot M48 contains connectors for the power cord and the computer cables. Do not touch this panel with wet hands. Environment...

  • Page 11: Biological Safety

    OSHA: Occupational Safety and Health Administration (United States of America) ACGIH: American Conference of Government Industrial Hygienists (United States of America) COSHH: Control of Substances Hazardous to Health (United Kingdom) QIAGEN BioRobot M48 01/2003...

  • Page 12: Chemicals

    Used labware, such as sample prep plates and disposable tips, may contain hazardous chemicals or infectious agents from the purification process. Such wastes must be collected and disposed of properly in accordance with the local safety regulations. QIAGEN BioRobot M48 01/2003...

  • Page 13: Mechanical Hazards

    Safety Information Mechanical hazards To protect users, the pipettor head of the BioRobot M48 will not move until the workstation door is closed. WARNING Moving parts [W7] To avoid contact with the moving pipettor head during operation of the BioRobot M48, do not insert your hands and fingers through the waste chute at the front of the instrument.

  • Page 14: Translations Of Warnings And Cautions

    WARNING (DANGER) La formule WARNING (DANGER) est utilisée pour avertir des situations pouvant occasionner des dommages corporels à l’utilisateur ou à d’autres personnes. Les détails sur ces circonstances sont données dans un encadré semblable à celui-ci. QIAGEN BioRobot M48 01/2003...
  • Page 15 L’utilisation non convenable du BioRobot M48 peut causer des blessures ou des détériorations de l’instrument. Le BioRobot M48 ne doit être utilisé que par du personnel qualifié qui a été formé de façon appropriée. Seul un ingénieur du service après-vente QIAGEN est autorisé...
  • Page 16 Lorsque l’instrument est relié au secteur, les raccords peuvent être sous tension, et des parties sous tension peuvent être découvertes en ouvrant des capots ou en retirant des pièces (à l’exception de celles auxquelles il est possible d’accéder manuellement). 1-10 QIAGEN BioRobot M48 01/2003...
  • Page 17 Safety Information WARNING Risk of electric shock [W3] The panel on the left side of the BioRobot M48 contains connectors for the power cord and the computer cables. Do not touch this panel with wet hands. Gefährdung durch Elektrizität Auf der linken Seite des BioRobot M48 befindet sich das Feld mit den Anschlüssen und Buchsen für die...
  • Page 18 Sicherheitsdatenblättern (MSDSs) oder in anderen zu beachtenden Dokumenten festgelegte Ausmaß überschreitet. Bei der Behandlung von Abluft und bei der Abfallbeseitigung sind alle gesetzlichen Regelungen zur Gesundheit und Sicherheit auf nationaler, regionaler und lokaler Ebene zu berücksichtigen. 1-12 QIAGEN BioRobot M48 01/2003...
  • Page 19 “Material Safety Data Sheets (MSDSs)” ou des documents “OSHA, ACGIH ou COSHH”. L’évacuation des vapeurs et déchets doit être conforme à tous règlements et dispositions légales - au plan national, départemental et local - concernant la santé et la sécurité. 1-13 QIAGEN BioRobot M48 01/2003...
  • Page 20 Sicherheitsdatenblättern (MSDSs) oder in anderen zu beachtenden Dokumenten festgelegt ist. Bei der Behandlung von Abluft und bei der Abfallbeseitigung sind alle gesetzlichen Regelungen zur Gesundheit und Sicherheit auf nationaler, regionaler und lokaler Ebene zu berücksichtigen. 1-14 QIAGEN BioRobot M48 01/2003...
  • Page 21 Sheets (MSDSs)” ou des documents “OSHA, ACGIH ou COSHH”. L’évacuation des vapeurs et déchets doit être conforme à tous règlements et dispositions légales - au plan national, départemental et local - concernant la santé et la sécurité. 1-15 QIAGEN BioRobot M48 01/2003...
  • Page 22 WARNING Moving parts [W7] To avoid contact with the moving pipettor head during operation of the BioRobot M48, do not insert your hands and fingers through the waste chute at the front of the instrument. Bewegliche Geräteteile Um den Kontakt mit dem sich bewegenden Pipettierkopf während des Betriebs des BioRobot M48 auszuschließen,...
  • Page 23 Risque de surchauffe Laisser un espace d’au moins 5 cm à l’arrière et 15 cm du côté gauche du BioRobot M48 pour assurer une ventilation efficace. Les grilles et prises d’air assurant la ventilation de la plate-forme BioRobot ne doivent pas être couvertes.
  • Page 24 Safety Information WARNING Risk of electric shock [W10] Do not open any panels on the BioRobot M48. Risk of personal injury and material damage Only perform maintenance which is specifically described in this manual. Gefährdung durch Elektrizität Unter keinen Umständen dürfen Gehäuseteile des BioRobot M48 geöffnet werden.
  • Page 25 [W12] Never install a fuse different from that specified in the user manual. Gefährdung durch Elektrizität Setzen Sie nur Sicherungen des im BioRobot M48 Handbuch angegebenen Typs ein. Risque d’électrocution Ne jamais installer d’autres fusibles que ceux indiqués dans le mode d’emploi.

  • Page 26: Symbols On The Biorobot M48

    Safety Information CAUTION Damage to the instrument [C1] Avoid spilling water or chemicals onto the BioRobot M48. Instrument damage caused by water or chemical spillage will void your Warranty. Beschädigung des Gerätes Vermeiden Sie es, Wasser oder Chemikalien auf der Oberfläche des BioRobot M48 zu verschütten.
  • Page 27 Moving part — during instrument head operation, the pipettor head may move suddenly Sich bewegende Geräteteile – beim Betrieb des BioRobot M48 kann es zu plötzlichen Bewegungen des Pipettierkopfes kommen Pièces mobiles — le bras pipetteur peut se déplacer rapidement sans prévention pendant que l’appareil...
  • Page 28 CE marking CE-Zeichen Marquage CE UL listing mark for Canada and the USA Symbol der UL-Zertifizierung in Kanada und den USA Certifié UL au Canada et aux Etats Unis Location of Symbols on the BioRobot M48 1-22 QIAGEN BioRobot M48 01/2003...

  • Page 29: Introduction

    Introduction Introduction Thank you for choosing the QIAGEN BioRobot M48 molecular biology workstation. We are confident it will become an integral part of your laboratory. Before using the instrument, it is essential to read this manual carefully and to pay particular attention to any advice it contains concerning hazards that may arise from use of this instrument.

  • Page 30: General Information

    Introduction General information 2.2.1 Policy statement It is the policy of QIAGEN to improve products as new techniques and components become available. QIAGEN reserves the right to change specifications at any time. In an effort to produce useful and appropriate documentation, we appreciate your comments on this publication.

  • Page 31: Intended Use Of The Biorobot M48

    BioRobot M48 for any particular use, since its performance characteristics have not been validated for any specific organism. The BioRobot M48 may be used in clinical diagnostic laboratory systems after the laboratory has validated their complete system as required by CLIA ‘88 regulations in the U.S.

  • Page 32: Requirements For Biorobot M48 Users

    This table covers the general level of competence and training necessary for transportation, installation, use, maintenance, and servicing of the BioRobot M48. QIAGEN provides training with the initial installation of the BioRobot M48. For additional training, contact QIAGEN Technical Services.

  • Page 33: Biorobot M48 - General Description

    The user selects a protocol in the QIAsoft M software and follows the on-screen instructions to load samples, reagents, and labware onto the worktable of the BioRobot M48. The user then closes the workstation door and starts the protocol, which provides all the necessary instructions for the BioRobot M48 to carry out the automated nucleic acid purification.

  • Page 34: External Features Of The Biorobot M48

    General Description External features of the BioRobot M48 Front View of the BioRobot M48 Workstation Workstation door Light switch Waste chute Slide table Status LEDs Connector panel Workstation door The workstation door can be manually opened by the user in order to gain access to the worktable.
  • Page 35 General Description The BioRobot M48 will try to move the pipettor head when you: • Open the QIAsoft M software — the pipettor head moves to the home position if it is not located there • Start a protocol If the workstation door is still open under these circumstances, the QIAsoft M software will display an error message.
  • Page 36 General Description Slide table A slide table is located at the bottom of the BioRobot M48 and can be pulled out from the front of the instrument. Slide Table NOTE: The slide table cannot support more than 5 kg. Connector panel...

  • Page 37: Internal Features Of The Biorobot M48

    General Description Internal features of the BioRobot M48 The interior of the BioRobot M48 contains the worktable and the pipettor head. 3.2.1 Worktable The worktable contains an array of slots for placing accessories, disposable labware, samples, and reagents. The BioRobot M48 Worktable...
  • Page 38 Holds up to 4 large reagent containers via the reagent container rack Rea_S Holds up to 6 small reagent containers via the reagent container rack Sample Holds a sample rack Tip Rack Holds a tip rack Waste Leads to the waste chute QIAGEN BioRobot M48 01/2003...
  • Page 39 General Description Accessories for the BioRobot M48 1. Tip rack 4. Sample rack 2. Reagent container rack 5. Waste chute 3. 48-tube cooling block for 2 ml tubes QIAGEN BioRobot M48 01/2003...
  • Page 40 A tip rack holds 84 disposable tips. Tip racks are compatible with the following polypropylene tips from QIAGEN: • Filter-tips, 1000 µl, M48 The BioRobot M48 worktable holds up to 3 tip racks (i.e., up to 252 disposable tips). QIAGEN BioRobot M48 01/2003...
  • Page 41 This is a safeguard to prevent the pipettor head from picking up the reagent containers. The BioRobot M48 tries to move the pipettor head when you: • Open the QIAsoft M software — the pipettor head moves to the home position if it is not located there •...
  • Page 42 General Description Sample rack A sample rack holds twelve 1.5 ml or 2 ml sample tubes. The BioRobot M48 worktable holds up to 4 sample racks. Sample racks are compatible with the following tubes: • 1.5 ml tubes without lids (Sarstedt cat. no. 72.696) •...
  • Page 43 48-tube cooling block for 0.2 ml tubes, which holds up to 48 PCR tubes. 48-tube Cooling Block for 0.2 ml Tubes (Assembled) 48-tube Cooling Block for 0.2 ml Tubes (Disassembled) Cover Aluminum base Plastic rack 3-11 QIAGEN BioRobot M48 01/2003...
  • Page 44 1. Remove the cooling block for 2 ml tubes. An aluminum base is located under the cooling block. 2. Remove the 2 screws that secure the aluminum base. 3. Use 2 hooks to remove the aluminum base. 3-12 QIAGEN BioRobot M48 01/2003...
  • Page 45 2 screws. 5. Place the plastic rack of the cooling block for 0.2 ml tubes onto the aluminum base. Load PCR tubes onto the plastic rack. 3-13 QIAGEN BioRobot M48 01/2003...

  • Page 46: Pipettor Head

    PCR tubes during the protocol run. Sample prep plates Sample prep plates are made of polypropylene and contain 42 wells each. The BioRobot M48 worktable holds up to 8 sample prep plates. Six samples are processed per sample prep plate. Thus, depending on the protocol, the BioRobot M48 processes up to 48 samples in a single protocol run.
  • Page 47 Since the syringe pumps operate simultaneously, this means that the BioRobot M48 processes 6, 12, 18, 24, 30, 36, 42, or 48 samples in one protocol run. 3-15...
  • Page 48 General Description 3-16 QIAGEN BioRobot M48 01/2003...

  • Page 49: Installation Procedures

    Requirements Site The BioRobot M48 must be located out of direct sunlight, away from heat sources, and away from sources of vibration and electrical interference. Refer to Appendix B for the operating conditions (temperature and humidity).

  • Page 50: Ac Power Connection

    Installation of AC power cord Connect one end of the AC power cord to the socket located on the left side of the BioRobot M48, and the other end to the AC power outlet. Repeat this procedure for the computer hardware.
  • Page 51 Installation Procedures The fuse box of the BioRobot M48 is located under the socket for the power cord and contains 2 fuses which are labeled as follows: • 110–120 V — an 8 A (250 V) fuse compatible with 110–120 V power supplies •...

  • Page 52: Hardware Installation

    Never install a fuse different from that specified in the user manual. Hardware installation Although installation of the BioRobot M48 is carried out by a QIAGEN Instrument Service Specialist, the following brief instructions are provided as reference. Unpacking the BioRobot M48 The following items are delivered: •...
  • Page 53 3. Install the slide table of the BioRobot M48. 4. Adjust the fuse box if necessary. 5. Connect the BioRobot M48 to the computer using the 2 communication cables. 6. Connect the BioRobot M48 and computer hardware to their power cords.

  • Page 54: Software Installation

    Installation Procedures Software installation Installation of the QIAsoft M software is performed either before delivery of the BioRobot M48 or by the QIAGEN Instrument Service Specialist at your installation site. Supplied with the BioRobot M48 are an installation CD-ROM for the QIAsoft M software and a diskette containing position teaching data that is specific for your instrument.

  • Page 55: General Operation

    General Operation This section describes how to operate the BioRobot M48. Before proceeding, it is recommended that you familiarize yourself with the features of the BioRobot M48 by referring to Section 3. Overview The steps for operating the BioRobot M48 are as follows: 1.

  • Page 56: Starting Up And Shutting Down The Biorobot M48

    Starting up and shutting down the BioRobot M48 5.2.1 Starting up the BioRobot M48 1. Switch on the BioRobot M48. The power switch is on the left side of the instrument. 2. Switch on the computer and its monitor. The following start-up window appears.

  • Page 57: Opening The Qiasoft M Software

    If you had closed the QIAsoft M software (Section 5.2.3), there are 2 alternative methods of opening it again: • Double-click the “QIAsoft M V2.0 for BioRobot M48” icon on the desktop • Click the Windows “Start” menu and select “QIAsoft M Operating System/QIAsoft M V2.0 for BioRobot M48”...

  • Page 58: Closing The Qiasoft M Software

    The QIAsoft M BIOS button • The QIAsoft M software button If the BioRobot M48 unexpectedly develops an error, click the QIAsoft M BIOS button to view the error log window. A description of the error log codes that appear in this window are provided in Section 7.2.2, page 7-3.

  • Page 59: Shutting Down The Biorobot M48

    1. Close the QIAsoft M software (Section 5.2.3). 2. Switch off the computer and its monitor. 3. Switch off the BioRobot M48 at its power switch. Main features of the QIAsoft M software The QIAsoft M software consists of a sequence of windows...
  • Page 60 O-ring Allows the O-rings of the pipettor head to be tested for Leakage Test leakage. Refer to Section 6.3.1 (page 6-7) for details on how and when to use this option. QIAGEN BioRobot M48 01/2003...
  • Page 61 NOTE: A default directory is already set for the sample name, report, and memo files. It is not necessary to change these default directories. If you select a submenu from the Setting menu, the following dialog box appears: QIAGEN BioRobot M48 01/2003...

  • Page 62: Protocol Selection Box

    Use this button to select the size of the elution tubes for Heat/Cool Block 2. Running a protocol Before starting, make sure that the BioRobot M48 is switched on and the QIAsoft M software is open (Section 5.2.1, page 5-2). If the first window of the QIAsoft M software (Page 1/16) is not displayed, click the “Cancel”...

  • Page 63: Selecting A Protocol

    NOTE: The displayed default values are used if no user input is made. 1. Click the dark green arrow in the protocol selection box and select a protocol from the menu that appears. NOTE: The handbook of the kit you are using indicates the required protocol. QIAGEN BioRobot M48 01/2003...
  • Page 64 6. In the “Set elution volume” dialog field, use the drop-down arrow to select the volume with which to elute each purified nucleic acid sample. 7. Click “Next” to move to the next window. 5-10 QIAGEN BioRobot M48 01/2003...

  • Page 65: Checking The Tip Guard Insert And Waste Chute

    There is sufficient space in the tip waste bag attached to the waste chute If necessary, refer to “Preventive Maintenance” (Section 6) for instructions on how to clean the tip guard insert and waste chute. Then click “Next” to move to the next window. 5-11 QIAGEN BioRobot M48 01/2003...

  • Page 66: Loading The Disposable Tips

    The text in the window indicates how many tips are required for the protocol. If there are enough tips on the worktable, click “No”. If there are not enough tips, fill the empty tip racks with tips and click the corresponding “Yes” buttons. 5-12 QIAGEN BioRobot M48 01/2003...

  • Page 67: Loading The Reagents

    If you use a reagent container that is too small, the disposable tips may hit the bottom of the container. If you use a reagent container that is too big, 5-13 QIAGEN BioRobot M48 01/2003...

  • Page 68: Importing And Exporting A Memo

    In this window (Page 5/16), you have the option of writing a memo in the text field. If you decide to sterilize the BioRobot M48 at the end of the protocol run, this memo appears again in the last window (Page 16/16).

  • Page 69: Loading The Sample Prep Plates

    After writing a memo (or not), click “Next” to move to the next window. 5.4.6 Loading the sample prep plates In this window (Page 6/16), the worktable graphic highlights the required sample prep plates in yellow. 5-15 QIAGEN BioRobot M48 01/2003...

  • Page 70: Loading Heat/Cool Block 1

    NOTE: Ensure that you use only 1.5 ml tubes from Sarstedt (see page 3-10). After loading the tubes, click “Next” to move to the next window. 5-16 QIAGEN BioRobot M48 01/2003...
  • Page 71 0.2 ml, 1.4 ml or 1.5 ml, or 2 ml. Also, ensure that the 1.5 ml or 2 ml tubes are from Sarstedt and that the correct cooling block is installed (see page 3-10). 5-17 QIAGEN BioRobot M48 01/2003...
  • Page 72 The order of the elution tubes shown above corresponds to the order of the sample tubes in the sample racks (see screenshot of Page 9/16 on page 5-19). After loading the tubes, click “Next” to move to the next window. 5-18 QIAGEN BioRobot M48 01/2003...

  • Page 73: Loading The Samples

    If you do not want to assign a name to each sample, click “No” to skip the next window (Page 10/16) and move to the one after it (Page 11/16). 5-19 QIAGEN BioRobot M48 01/2003...
  • Page 74 Entering sample names Enter the sample names into the active dialog fields by using the keyboard. Alternatively, you can enter the sample names by using an optional bar code reader (see Appendix B for specifications). 5-20 QIAGEN BioRobot M48 01/2003...
  • Page 75 Click “Save” to save the sample names. The saved file contains the protocol name, the date and time of creation, and the sample names numbered from 1 to 48 (each number indicates the position on the worktable). 5-21 QIAGEN BioRobot M48 01/2003...

  • Page 76: Confirming The Worktable Layout

    If the worktable layout is correct: 1. Click “Confirm”. A dialog box appears and asks you to close the workstation door. 2. Close the workstation door and click “OK” in the dialog box. 3. Click “Go” to start the protocol. 5-22 QIAGEN BioRobot M48 01/2003...

  • Page 77: Checking The Status Of The Protocol Run

    Dialog field Description Start Time The time at which the protocol run was started. Remaining The time remaining until the protocol run ends. Time Completion The estimated time at which the protocol run ends. 5-23 QIAGEN BioRobot M48 01/2003...
  • Page 78 When you click this button, a confirmation dialog box appears. Click “Yes” to end the protocol run and display the results. Click “No” to continue the protocol run undisturbed. When the protocol run is finished, the next window automatically appears. 5-24 QIAGEN BioRobot M48 01/2003...

  • Page 79: Viewing The Results Of The Protocol Run

    Within each group, there is a dialog field that shows whether the samples have passed or failed. If there is a failure, an error code indicates the type of failure (Section 7.2.3, page 7-8). 5-25 QIAGEN BioRobot M48 01/2003...
  • Page 80 Error 21 Fail Error 21 A dialog box also appears at the end of the protocol run, and prompts you to sterilize the BioRobot M48. Remove the elution tubes containing your purified nucleic acids, and do one of the following: •...

  • Page 81: Sterilizing The Biorobot M48

    This window (Page 14/16) gives you the option of sterilizing the interior of the BioRobot M48. If you want to close the QIAsoft M software without sterilizing the BioRobot M48, click “No”. A confirmation dialog box appears: • Click “Yes” to close the QIAsoft M software •...
  • Page 82 General Operation If you want to sterilize the BioRobot M48: 1. If the worktable contains some tip racks with unused tips, transfer these racks to slots that will allow the tips to be used up in the next protocol. The disposable tips can tolerate one UV treatment.
  • Page 83 During sterilization, you have the option of editing the memo you prepared in Section 5.4.5, page 5-14. If desired, use the “Import” button to load a memo or the “Export” button to save the memo. For details, see Section 5.4.5. 5-29 QIAGEN BioRobot M48 01/2003...
  • Page 84 If the keyboard is not touched for 5 minutes during sterilization, a screen saver appears. This screen saver displays the time remaining for sterilization and the text of the memo. Move the mouse to remove the screen saver. 5-30 QIAGEN BioRobot M48 01/2003...

  • Page 85: Preventive Maintenance

    Preventive Maintenance Preventive Maintenance The following preventive maintenance procedures must be carried out to ensure reliable operation of the BioRobot M48: • Daily preventive maintenance — after the last protocol run of the day • Weekly preventive maintenance — every week •...

  • Page 86: Daily Maintenance Procedure

    Contact your local QIAGEN Instrument Service representative, or your local distributor for more information about flexible Service Support Agreements from QIAGEN. Daily maintenance procedure WARNING Hazardous chemicals and infectious agents [W11] The waste contains samples and reagents.
  • Page 87 If the worktable contains some tip racks with unused tips, transfer these racks to slots that will allow the tips to be used up in the next protocol. The disposable tips can tolerate one UV treatment. QIAGEN BioRobot M48 01/2003...

  • Page 88: Cleaning The Tip Guard Insert

    6.1.1 Cleaning the tip guard insert Clean the tip guard insert if it becomes dirty: 1. Switch on the BioRobot M48 and open the QIAsoft M software (Section 5.2.1, page 5-2). 2. Select “Manual Operation/Drop Catcher Cleaning”. The pipettor head moves to the front of the worktable, the tip guard moves forward and becomes accessible, and the workstation door unlocks.

  • Page 89: Greasing The O-Rings

    Greasing the O-rings Greasing the O-rings of the pipettor head is required during the weekly maintenance procedure. 1. Switch on the BioRobot M48 and open the QIAsoft M software (Section 5.2.1, page 5-2). 2. Select “Manual Operation/Greasing O-rings”. The pipettor head moves to the front of the worktable, the 6 tip adapters move down and become accessible, and the workstation door unlocks.

  • Page 90: Replacing The O-Rings

    Replacement of the O-rings of the pipettor head is required if they look worn-out or damaged during the weekly maintenance procedure. 1. Switch on the BioRobot M48 and open the QIAsoft M software (Section 5.2.1, page 5-2). 2. Select “Manual Operation/Greasing O-rings”.

  • Page 91: Monthly Maintenance Procedure

    Performing an O-ring leakage test An O-ring leakage test is required during the monthly maintenance procedure. 1. Switch on the BioRobot M48 and open the QIAsoft M software (Section 5.2.1, page 5-2). 2. Select “Manual Operation/O-ring Leakage Test”. A dialog box with instructions appears. You will need to:...
  • Page 92 Preventive Maintenance QIAGEN BioRobot M48 01/2003...

  • Page 93: Troubleshooting

    Troubleshooting Troubleshooting Refer to this section if you encounter problems while operating the BioRobot M48. The following items are described: • General troubleshooting • Errors displayed in the QIAsoft M software General troubleshooting Error Cause/Remedy BioRobot M48 cannot BioRobot M48 is not receiving power.

  • Page 94: Errors Displayed In The Qiasoft M Software

    5. Make a backup of the following file: C:\Program Files\GenoM-48\Tmp\bbios.log It is recommended that you rename this backup as: bbiosyymmdd.log where yy is the year, mm is the month, and dd is the day. QIAGEN BioRobot M48 01/2003...

  • Page 95: Types Of Error Log Codes

    7.2.2 Types of error log codes If the BioRobot M48 unexpectedly develops an error, click the QIAsoft M BIOS button to view the error log window (Section 5.2.4, page 5-4). Error log codes follow the format X M S EEE, where: •...
  • Page 96 NOTE: Error codes 1–8 refer to the opening of the QIAsoft M software. Therefore, there is no error level. Code Level Label*1 Message — ERR_BIOS_INITIALIZE Failed to initialize BIOS — ERR_IF_BIOS_NOT_EXEC Cannot execute SX BIOS.EXE — ERR_IF_OPEN_MAPPING OpenFileMapping = NULL QIAGEN BioRobot M48 01/2003...
  • Page 97 Z height ERR_MOTOR_NOT_BINARY Do not support binary axis calculation mode ERR_INVALID_MODULE_NO Invalid module number ERR_INVALID_ITEM_NO Invalid item number ERR_NO_TIP Cannot find tip ERR_INVALID_PARAMETER Invalid parameter ERR_INVALID_USER_PARAM Invalid user parameter ERR_INVALID_IN_PORT Cannot use this IN port address QIAGEN BioRobot M48 01/2003...
  • Page 98 Tip did not connect ERR_INVALID_VOLUME Invalid volume set ERR_INVALID_VOL_TABLE Invalid volume table ERR_1000P_OVER Cannot assign more than 1000 pulses ERR_NDEF_NAME [%s] name= not defined ERR_INVALID_PORT Invalid port number ERR_USERSTOP_MOTOR User stopped motor ERR_VOLUME_OVER Liq in Volume overflow QIAGEN BioRobot M48 01/2003...
  • Page 99 Invalid motor name ERR_INVALID_TOOL Invalid tool number ERR_INVALID_VPTABLE Invalid volume-pulse table ERR_HEATBLOCK_ALARM Heat/Cool Block 1 temperature is out of range ERR_COOLBLOCK1_ALARM Heat/Cool Block 2 temperature is out of range ERR_VENTILATOR_ALARM Ventilator fan is stopped ERR_TIP_UNEJECT Tip cannot eject QIAGEN BioRobot M48 01/2003...

  • Page 100: Common Error Codes

    This error indicates that the QIAsoft M software cannot communicate with the BioRobot M48. Ensure that the cables between the computer and the BioRobot M48 are secure. If the problem persists, contact QIAGEN Technical Services. Error 10: Motor stopped emergently This error indicates that the limit sensors have been triggered and that one of the motors has stalled.
  • Page 101 • The sample racks are at the wrong positions If you cannot resolve the error, write down the events leading to Error 21, save the system log (Section 7.2.1, page 7-2), and contact QIAGEN Technical Services. QIAGEN BioRobot M48 01/2003...
  • Page 102 2. Check whether the disposable tip attached to tip adapter number 1 is blocked. 3. If the error is not due to the last 2 steps, save the system log (Section 7.2.1, page 7-2) and contact QIAGEN Technical Services. Error 35: User stopped motor This error indicates that the user had stopped the QIAsoft M software.
  • Page 103 Error 52: Ventilator fan is stopped This error indicates that the ventilator fan has stopped. The BioRobot M48 cannot operate without the fan, and stops operating immediately. Click the “Continue” button in the error dialog box that appears.
  • Page 104 Troubleshooting 7-12 QIAGEN BioRobot M48 01/2003...

  • Page 105: Glossary

    Glossary Glossary Word Description Connector The panel on the left side of the BioRobot M48. It contains panel the power switch, the socket for the power cord, the fuse box, and the connectors for the computer cables. Cooling and A component of the BioRobot M48 that accommodates a heating cooling block and cools or heats the tubes in the block.
  • Page 106 A ring that is fitted at the bottom of a tip adapter. It is required for good contact between the tip adapter and a disposable tip. Pipettor head The component of the BioRobot M48 that aspirates and dispenses liquid and separates MagAttract magnetic particles. The pipettor head moves to different locations above the worktable and contains 6 syringe pumps, each of which is connected to a tip adapter.
  • Page 107 Tip waste bag The plastic bag that is attached to the waste chute and collects used disposable tips. Waste chute A metal slide at the front of the BioRobot M48. It receives disposable tips discarded by the pipettor head, and transfers them to an attached tip waste bag.
  • Page 108 Glossary QIAGEN BioRobot M48 01/2003...

  • Page 109: Technical Assistance

    Appendix A Appendix A Technical assistance At QIAGEN we pride ourselves on the quality and availability of our technical support. Our Technical Services Departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of QIAGEN products.
  • Page 110 Appendix A QIAGEN BioRobot M48 01/2003...

  • Page 111: Technical Data

    Humidity <80% relative humidity at 30ºC (86ºF) or below Altitude Up to 2000 m (6500 ft.) Place of For indoor use only operation Storage –40ºC to 65ºC (–40ºF to 149ºF) temperature Pollution level Overvoltage category QIAGEN BioRobot M48 01/2003...
  • Page 112 Windows operating system • Temperature control through 2 Peltier cooling and heating systems • Interlocks are activated and prevent pipettor head movement if the workstation door and the latches for the reagent containers are not closed QIAGEN BioRobot M48 01/2003...
  • Page 113 Pipettor head moves in the X, Y, and Z directions to any location on the worktable. Pipettor head contains a tip guard which collects any aspirated liquid that may drip from the disposable tips during movement of the pipettor head above the worktable. QIAGEN BioRobot M48 01/2003...
  • Page 114 Attach to the tip adapters of the pipettor head to allow liquid tips aspiration and dispensing. Capacity of 1200 µl. The BioRobot M48 accommodates up to 3 tip racks (84 tips per rack). Maximum capacity is 252 tips. QIAGEN BioRobot M48 01/2003...
  • Page 115 Waste chute Used disposable tips are discarded through the waste chute into an attached tip waste bag. The waste chute is made of metal and is autoclavable. The tip waste bag is supplied by the user. QIAGEN BioRobot M48 01/2003...
  • Page 116 Appendix B QIAGEN BioRobot M48 01/2003...
  • Page 117 LOSSES (INCLUDING LOSS OF USE, LOST REVENUES, LOST PROFITS AND DAMAGE TO REPUTATION), EVEN IF SUCH DAMAGES WERE FORESEEN OR FORSEEABLE, OR WERE BROUGHT TO QIAGEN’S ATTENTION. IN NO EVENT SHALL QIAGEN’S LIABILITY TO YOU EXCEED THE PURCHASE PRICE OF THE PRODUCT.
  • Page 118 Appendix C Liability clause QIAGEN shall be released from all obligations under its warranty in the event repairs or modifications are made by persons other than its own personnel, except in cases where the Company has given its written consent to perform such repairs or modifications.
  • Page 119 Pipettor head, 3-1, 3-14 Power cord, 3-4 Heat/Cool Block 1, 3-10 Power requirements, 4-2 loading, 5-16 Power switch, 3-4 Heat/Cool Block 2, 3-10 Protocol, 3-1 loading, 5-17 checking status, 5-23 running, 5-8 selecting, 5-9 viewing results, 5-25 Index-1 QIAGEN BioRobot M48 01/2003...
  • Page 120 1-8 Sample rack, 3-10 Waste chute, 3-2, 3-3 Sample tubes, 3-10 checking, 5-11 Samples Waste disposal, 1-6 loading, 5-19 Workstation door, 3-1, 3-2 Servicing, 6-1 Worktable, 3-1, 3-5 Shutting down, 5-5 confirming layout, 5-22 Index-2 QIAGEN BioRobot M48 01/2003...
  • Page 121 QIAGEN Companies Please see the inside front cover for contact information for your local QIAGEN office. QIAGEN Distributors Argentina Egypt New Zealand Taiwan Tecnolab S.A. Clinilab Biolab Ltd TAIGEN Bioscience Corporation Charlone 144 - C1427BXD P.O. Box 12 El-Manial 244 Bush Road 3F.

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