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As it is not possible to see the distance between the tip and the base in most cases, the distance must be "felt" by manually
lifting the well from its support.
11. If the (the pipetting head) is accidentally moved too far downwards in the Z direction (i.e., touches the base), ensure that
the pipetting head is returned to the proper position by clicking Home Z-axis.
12. When the selected "Level at bottom of well" position is suitable, click OK to set the correct height calibration.
13. Once all plates have been height calibrated, click Close to exit the wizard.
Calibrating volumes
A direct relationship exists between the rotation of the motor shaft (P-axis) and the vertical movement of the pipet piston (i.e.,
volume aspirated or dispensed). Volume calibration defines the number of units that the P-axis motor must move to accurately
deliver a given volume of liquid.
Volume calibration is performed using the "Volume calibration" wizard. During installation, the preinstalled settings can be set
up using "Option 1" from the "Volume calibration" wizard.
Before starting the first run on a newly installed QIAcube HT volume calibration has to be done. If no volume calibration has
been done an error is shown at start of a run.
Opening the "Volume calibration" wizard
1. Start the QIAcube HT Software.
For more details, see Section 5.2, page 48.
2. From the options that appear, select Empty Project (last used layout) or Empty Project (default layout).
3. From the Options menu in the menu bar, select Robot Setup/Calibrate Volumes.
The "Volume Calibration Wizard" dialog box appears.
QIAcube HT Version 4.17 User Manual
06/2023
97
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