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Accuris AutoMATE 96 Operation Manual

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Operation Manual
V1.0
MP9604
This Product is for Research Use Only

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Summary of Contents for Accuris AutoMATE 96

  • Page 1 Operation Manual V1.0 MP9604 This Product is for Research Use Only...
  • Page 2 Please inspect the instrument as well as all included accessories when you first open the packaging. If you find anything damaged or missing, please contact Benchmark Scientific or your local distributor immediately. BENCHMARK SCIENTIFIC / ACCURIS INSTRUMENTS PO Box 709 Edison, NJ 08818 Phone: 908-769-5555 Website: www.benchmarkscientific.com / www.accuris-usa.com...
  • Page 3 To prevent injury or voiding the warranty, the operator should not attempt to repair the instrument without explicit guidance from Accuris Instruments. If service is required, please contact Accuris Instruments or your local distributor for repair. Before powering on, confirm that the voltage used meets the electrical requirements of the instrument as stated on the rating plate.
  • Page 4 Pull the connector plug from the electrical outlet immediately and contact the vendor in the event of: • Liquid entering the housing. • Abnormal operation: such as any abnormal sound or smell. • The instrument is dropped or there is any damage to the housing.

  • Page 5: Table Of Contents

    Table of Contents Chapter 1 Introduction ...................... 1 1. Key Features........................1 Chapter 2 Specifications ....................2 1. Required Installation Environment .................. 2 2. Instrument Specifications....................2 3. Pipetting Head Specifications ..................3 Chapter 3 Instrument Overview ..................4 1. Structure .......................... 4 2.

  • Page 6: Chapter 1 Introduction

    Three interchangeable heads allow for a pipetting range from 1.0µL to 1000µL. The AutoMATE 96 is compact enough to fit onto a standard lab bench or into a biosafety cabinet. 1. Key Features ➢...

  • Page 7: Chapter 2 Specifications

    Plate Automatic / motorized positioning Operation Tablet, basic mannual operation and progremmed operation interface Channels 8 / 96 Compatible Tips Accuris AutoMATE tips Operating Android Display 8 inch tablet, color display Instrument port USB B Power supply 100 to 240VAC, 50/60Hz, 100W...

  • Page 8: Pipetting Head Specifications

    3. Pipetting Head Specifications MP9600-20PH MP9600-200PH MP9600-1000PH Pipette Head (20 µL) (200 µL) (1000 µL) Number of pipetting channels Pipetting 1~20 5~200 100~1000 range/µL 1µL: ≤±12% 5µL: ≤±5% 100µL: ≤±2.5% 2µL: ≤±5% 20µL: ≤±2% 500µL: ≤±1% Pipetting accuracy 10µL: ≤±1% 100µL: ≤±1% 1000µL: ≤±1% 20µL: ≤±1%...

  • Page 9: Chapter 3 Instrument Overview

    Chapter 3 Instrument Overview 1. Structure Fig 1. AutoMATE 96 Structure 1) Shell 2) Front Casing 3) Tablet 4) Pipetting Head 5) Front Plate 6) Motorized Platform 7) Operational LED Indicator 8) SWITCH (STOP) Key 9) Base 10) USB Port...

  • Page 10: Dimensions

    Dimensions Fig 1. Dimensions...

  • Page 11: Chapter 4 Instrument Setup

    Packing List. Should there be any doubts or questions about the instrument or its accompanying accessories, such should be recorded and Accuris Instruments or the relevant distributor should be contacted.

  • Page 12: Pipette Head Installation / Replacement

    2. Pipette Head Installation / Replacement ① Push front casing up ② Remove the front edge ③ Loosen the locking levers ④ Replace pipetting head ⑤ Tighten levers ⑥Replace front casing & front edge Fig 3. Pipette Head Replacement...

  • Page 13: 4-Well Microplate Pipetting

    During a program run, the AutoMATE 96 will dispense into two horizontal positions, and then instruct the user to move the 384-well microplate to the top left side of the adapter to dispense into...

  • Page 14: Chapter 5 Programming & Operation

    Chapter 5 Programming & Operation 1. Software Initialization On the included Android Tablet, select the “AutoMATE™ 96” app icon to enter the application interface. Fig 3. AutoMATE™ 96 App Icon Select the user (Admin or other), enter the corresponding user password, and click “Login”...

  • Page 15: App Main Interface

    2. App Main Interface After logging in, the main interface will display (Figure 8). On the main interface, choose one of the two pipetting modes: Manual Mode or Automated Program Mode, as shown in the figure below. Fig. 5 AutoMATE™ 96 Application Main Interface...

  • Page 16: Manual Mode

    3. Manual Mode Choose "Manual mode" to enter the basic mode, as shown in the figure below. Fig. 6 Manual Mode Interface Platform Positions: The motorized platform has four positions: P1 corresponds to platform position 1, P2 corresponds to platform position 2, and so on. To change to another platform in the Manual Mode Interface, select a different platform position to highlight it blue (Figure 10);...
  • Page 17 “Eject Tips” icons to highlight it blue (Figure 11); the instrument will automatically load / eject tips to the currently selected platform position. Fig. 8 Manual Mode: Tip Loading & Ejection Pipetting Functions In the Manual Mode Interface, users can toggle amongst settings for “Aspirate” , “Dispense”, and “Mix”...
  • Page 18 Table 1 Legend: Manual Mode Pipetting Functions Aspiration volume Click the Aspirate volume text box, enter the volume of liquid to be (µL) aspirated. Dispensation Click the Dispense volume text box, enter the volume of liquid to be volume (µL) dispensed.
  • Page 19 Manual Mode: Function Keys As shown in the figure below, there are four Function Keys available : Aspirate, dispense, Mix and Blow out. The current set volume is shown in the upper left corner of each Operation button. If the Operation Setting has been set with automatic aspiration/dispense/mix with a position (mm) set, this set position will also be displayed in the upper left corner.

  • Page 20: Automated Progam Mode

    4. Automated Program Mode Choose “Automated Program Mode” from the upper left menu to enter the Automated Program Mode, as shown in the figure below. Fig 12 Automated Program Mode Interface The Automated Program Mode offers four function buttons at the top right: New, Save as, Manage and Records.
  • Page 21 Fig 13 New protocol Fig 14 Select step The “Step Parameters” for the newly added step can now be set. Select a Position for the step (P1 to P4). Typically, “Load Tips” would be the first step, and for this the tips are usually positioned the at P1 (far left) position of the platform.
  • Page 22 Fig 17 Labware list Fig 18 S1 P1 Load Tips After setting up the step parameters, click on “New Step” to add another step. The program can be saved by pressing the disk icon in the upper right of the screen. Steps can be modified by pressing any of the created steps in the upper left.
  • Page 23 Automated Program Mode: Aspirate & Dispense Settings: Figure 19 Aspirate & Dispense Settings Aspiration Step Parameters: The following settings are available and adjustable for the aspiration steps: • Position: Platform position (P1 – P4) • Labware List: Select from which plate aspiration will occur (96 deep well plate, reagent reservoir, etc.) •...
  • Page 24 AutoMATE 96 will dispense • Bottom (mm): Adjust How far from the bottom of the dispense position selected the AutoMATE 96 will dispense • Post-Mix: Toggle to enable mixing of the reagent post-dispensation o Mix Volume (µL): The volume of the reagent that will be mixed after...
  • Page 25 dispensation • Deviation Value: Adjust the vertical positioning of the point of aspiration. This feature does not require input if performing basic aspiration from the selected Labware List. • Column Dispense: Toggle to enable column dispensation. Note: this feature is only available when “Column Load” and “Column Aspirate” are toggled on in the Load Tips / Aspiration steps (see Figures 18 &...
  • Page 26 • Top (mm): Adjust How far from the top of the Rinse position selected the AutoMATE 96 will perform internal/external rinsing. • Bottom (mm): Adjust How far from the bottom of the Rinse position selected the AutoMATE 96 will perform internal/external rinsing. Automated Program Mode: Cycle Settings: Figure 22 Cycle Settings...

  • Page 27: Protocol Management / Settings

    Cycle Step Parameters The following settings are available and adjustable for the cycle step: From Step: Select from which step the cycle will begin from. To Step: Select which step the cycle will end on. Repeat: Input the # of times the cycle step will perform Example: •...
  • Page 28 Protocol Preview To Preview the detailed steps of a saved protocol, users can highlight the protocol and click the Preview button (as shown in Figure 37). From the preview screen, the user can click the Run button to start the protocol or press the < icon at the top left of the screen to return to the protocol list screen.
  • Page 29 Fig 28 Run Interface Stop / Pause a Run in Progress During a protocol run, the user can Stop the process by clicking the Stop button. A window will appear to confirm the stopping of the run (as shown in Figure 40) and clicking OK will stop the current protocol.
  • Page 30 Pipette Volume is 0.0 (no liquid in the tips) then the SWITCH button will activate aspiration. If the Current Pipette Volume is >0.0 (there is liquid in the tips) then the SWITCH button will activate dispensing. 2. In Automated Program Mode, the function of the SWITCH button can be used to start a run (same as Run button), and when a program is running the SWITCH button can be used to Pause the run.
  • Page 31 Fig 31 Settings Parameter Settings – General Interface Parameters setting allows adjustments to Liquid parameter, Speed parameter and Default tips, as shown in the figure below. Fig 3216 Parameter interface Liquid Parameter Settings: Different liquids require different pipetting parameters for accurate aspiration and dispensing.
  • Page 32 Fig 33 Liquid Parameter Management Interface Speed Parameter Settings: Fig 34 Speed Parameter The speed parameter interface allows adjusting the speed and acceleration values of the X axis (motorized platform) and Z axis (pipetting head).

  • Page 33: System Settings

    Default Tips’ Settings: Fig 35 Default Tips Interface Default tips interface can set default for tips. System Settings System settings are for selecting Language, Time, Screen brightness, Firmware Upgrades and Bluetooth options . For firmware upgrades, insert a USB drive with updated firmware files to perform updates ( Fig 0.) Fig 36 System settings interface...
  • Page 34 Fig 38 Bluetooth Settings Fig 39 Screen brightness settings Figure 40 System Upgrade Settings Fig 41 Upgrade settings Fig 42 Reset settings Fig 43 Bluetooth settings Fig 44 Restore the factory setting...
  • Page 35 User Settings Account Admin Switch User Fig 53 User settings interface Clicking on the user name in the upper left corner to see user options, including Account Administration and Switch User, as shown in the image above. Per Fig , the Account Admin icon to enter user settings interface. Click “Password” to change the Admin password and click “Manage”...
  • Page 36 Fig 55 User manage interface Fig 56 Change username interface Fig 57 Reset password Interface Fig 58 Delete interface...
  • Page 37 Fig 59 Add user interface Table 2 Function Click to change the current user name (maximum of 16 characters can be used); See Fig Click to change the password for the user (maximum of 10 characters, including digits and case-sensitive letters) See Click, to delete a user.
  • Page 38 Change User Account Per Fig , click the “Switch user” icon to switch to a different user account. Fig 60 Change interface...

  • Page 39: Labware Management

    8. Labware Management From the main settings menu, click “Labware”, to enter labware management interface, as shown figure below. Figure 47 Labware Management Fig 48 Labware management interface Fig 49 Labware new well plate interface...
  • Page 40 To add a new consumable that can be selected during programming, choose the type of consumable listed under "Labware", then click the “new” box which is the box with the “+” in the center (see Fig ); A brand can be selected or a new brand can be added. After entering a new brand and saving the newly created consumable, the new brand will be automatically saved as an option for future selection (see Fig );...

  • Page 41: Chapter 6 Maintenance

    Chapter 6 Maintenance 1. Suggested Maintenance Tools The following tools are suggested for routine maintenance work: A set of 1.5-6mm hex (Allen) keys , Phillips head screwdriver (large, medium, small), small tweezers, clean gauze, clean cotton swabs, clean brush, rubber gloves, 75% alcohol and cleaning fluid, etc.

  • Page 42: Annual Maintenance

    4. Annual Maintenance Contact the Accuris Instruments, or your authorized distributor or authorized service provider, for any necessary maintenance, including but not limited to the following items: a. Check that the moving platform is level and operating smoothly. b. Check the pistons and seals to make sure there is no leakage, and that pipetting volumes are consistent between channels.

  • Page 43: Chapter 6 Troubleshooting

    Tablet not Power Switch failure Replace switch connecting to Instrument Fuse blown Replace fuse Contact distributor or Other Accuris Service Dept. Contact distributor or Controller failure Accuris Service Dept. Instrument does not operate Contact distributor or Motor failure Accuris Service Dept.
  • Page 44 Failure module Failure name Error E109 Over temperature pre-warning. E119 Over temperature. Short circuit in motor. E107 Failed to leave zero. E413 Failed to move to destination. E114 Failed to return zero. E403 X-axis Memory is damaged. E713 Motor data is lost. E723 Motor data error.

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Mp9604

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