Accuris isoPURE Mini Operation Manual
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Accuris isoPURE Mini Operation Manual

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Operation Manual
V1.2
AP1016

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Summary of Contents for Accuris isoPURE Mini

  • Page 1 Operation Manual V1.2 AP1016...
  • Page 3 Please inspect the instrument as well as all included accessories when you first open the packaging. If you find anything damaged or missing, please contact Benchmark Scientific or your local distributor immediately. BENCHMARK SCIENTIFIC / ACCURIS INSTRUMENTS PO Box 709 Edison, NJ 08818 Phone: 908-769-5555 Website: www.benchmarkscientific.com / www.accuris-usa.com...
  • Page 4 To prevent injury or voiding the warranty, the operator should not attempt to repair the instrument without explicit guidance from Accuris Instruments. If service is required, please contact Accuris Instruments or your local distributor for repair. Before powering on, confirm that the voltage used meets the electrical requirements of the instrument as stated on the rating plate.
  • Page 5 Pull the connector plug from the electrical outlet immediately and contact the vendor in the event of: • Liquid entering the housing. • Abnormal operation: such as any abnormal sound or smell. • The instrument is dropped or there is any damage to the housing.

  • Page 6: Table Of Contents

    Table of Contents Chapter 1 Introduction ......................1 1. Key Features ..........................1 Chapter 2 Specifications ......................2 1. Required Installation Environment ................... 2 2. Specifications ........................... 2 3. Dimensions ..........................3 Chapter 3 Instrument Overview ....................4 1. Structure ........................... 4 2.

  • Page 7: Chapter 1 Introduction

    Chapter 1 Introduction The IsoPure™ Mini is an automated purification system for DNA/RNA, proteins, and cells. It can bind, transfer and release magnetic beads by using moveable magnetic rods. Users can extract 1~16 samples simultaneously with appropriate magnetic bead kits and reagents available from many suppliers. The IsoPure™ series can be used with samples from animal/plant tissue, blood, and body fluids.

  • Page 8: Chapter 2 Specifications

    Chapter 2 Specifications 1. Required Installation Environment Environmental Temperature: 10°C～40°C Relative Humidity: ≤ 80% Input Voltage: AC 100～240V, 50Hz/60Hz 2. Specifications Model IsoPure™ Mini Display 4.3-inch touch screen Purification Method Magnetic Bead-Based Purification Sample Volume 50μL—1000μL Up to 16 samples using deep well plates or the Throughput included aluminum rack Stability...

  • Page 9: Dimensions

    UV Sterilization UV Lamp (3W UVC, 253.7 λ) Magnetic Bead Recovery > 95% Internal Lighting LED lamp Power Standby 10W, MAX 150W Dimensions 20.8cm x 25.8cm x 31.5cm Weight 7 kg / 15.4 lbs 3. Dimensions Fig 1. Dimensions Dimensions (W×D×H): 7.9x10.2x11.8 in / 208×258×315mm 258mm / 10.2 in 208mm / 7.9 in...

  • Page 10: Chapter 3 Instrument Overview

    Chapter 3 Instrument Overview 1. Structure Front Operational LED Indicator Chamber Door Fig 2. Front Back The IsoPure™ Mini is a newly launched automated extraction and purification system for DNA/RNA, proteins, and cells. It can absorb, transfer, and release magnetic beads via magnetic rods. Users can extract 1~16 samples simultaneously with a 96-Deepwell plate.
  • Page 11 Side USB Port Fuse PC Port Fig 4. Side Sample Processing Chamber UV lamp LED lamp (top of processing chamber) Magnetic rod Tip Comb assembly holder housing Tip Combs 96 deep well plate Cooling fan (underneath heating block) Fig 5. Sample Processing Chamber...

  • Page 12: Control Panel

    2. Control Panel 4.3-inch touch screen STOP Fig 6. Touch screen control panel Touch screen: For operational control (mouse also can be connected for operation). TAB: Switch between protocols. RUN: Start the protocol. STOP: Stop the protocol.

  • Page 13: Chapter 4 Instrument Setup

    Chapter 4 Instrument Setup 1. Preparation before use Remove the instrument from the shipping box. To release the horizontal motor, use the included Allen key to unscrew the M6 hexagonal screws found underneath the instrument. Remove the tape holding the chamber door to open the door. Remove the zip ties between the magnetic rod assembly and the tip comb holder.

  • Page 14: Deep Well Plate Installation

    2. Plate Installation 96 Deep Well Plate Installation Open the chamber door. First, place the plate in the position according to the blue arrow ①. Then press the plate into the heating block according to the arrow ②. Note the orientation of the plate (plate corner A1 should correspond with the mark A1 on the base of the processing chamber) to avoid the wrong sequence of reagents.

  • Page 15: Tip Comb Installation

    3. Tip Comb Installation Slide the tip combs into the grooves of the tip comb holder following the arrow in Fig. Tip comb Fig 11. Tip comb installation 4. Removing Tip Combs Press down the tab found on the tip comb’s handle according to the blue arrow ①. While pressing down the tab, pull the tip combs in the direction of the blue arrow ②.

  • Page 16: Chapter 5 Programming & Operation

    Chapter 5 Programming & Operation Protocol programming is done using the Android App software which is saved on the included USB flash drive. Protocols are then transferred to the instrument where they are saved in the internal memory and can be selected for operation. For users that do not have an Android device, PC software can also be used for protocol programming.

  • Page 17: App Main Interface

    IsoPure Mini Create new Create and edit a protocol new protocol Acquire Download protocols protocol from IsoPure Mini from onto app instrument Instrument Connect the Android Bluetooth device to the IsoPure connection Mini via Bluetooth Fig 15.
  • Page 18 Instrument Acquisition: Shows a list of protocols saved in the internal memory of the instrument. Protocols can be selected for transmission to the app. Fig. 16: Protocol Select and Transmit Interface Local Creation: Select a protocol or multiple protocols (Fig. 17, Fig. 18). 4 buttons will appear at the bottom of the screen: Transmit, Delete, Display QR Code, and Save as.
  • Page 19 Transmit: Transfer the selected protocols from the app to the instrument. Delete: Remove protocols from app memory. Display QR Code: Generate the protocol specific QR code. Use the included handheld scanner to instantly transfer the protocol to the instrument. Press “Save” when the code is displayed to save the code to the photo gallery on the Android device (Fig.
  • Page 20 Preview: Select the blue “Preview” button to the right of a protocol to see the protocol steps with details (Fig. 20). Fig. 20: Preview of protocol steps New protocol Click the "New" icon on the “Protocol select and transmit” interface to create a new protocol (Fig.
  • Page 21 After the new protocol is named, the interface will allow for the addition of new steps (Fig. 22). Users can add, delete, or copy a step. After the protocol steps have been set, users can preview, edit, and rearrange protocol steps. Note: A new protocol can also be created from the “Create new protocol”...
  • Page 22 Move: Adjusts the sequence of steps. Hold and drag the “Move” icon to adjust the sequence of steps (Table 2, Fig. 24). Fig. 23 Select to Delete or Fig. 24 Move the sequence Copy steps of steps Table 2 New Protocol Interface Legend Icon Name Definition...
  • Page 23 Step parameters settings Select a step from the list of steps of a protocol (press the middle of the bar) to enter the parameter settings interface (Fig. 25). At the top, the current sample-well position is shown (position 1-6). To change the position of the step, highlight another position number in orange.
  • Page 24 Table 3 Parameter Settings Legend General Parameter Definition Settings Name of step. The default steps cannot be renamed. Step Name Only a custom step can have a custom name. Custom step parameters are shown in Fig. 25. The position site corresponds to columns of the plate (position 1 = column 1 on plate), the positions for Position default steps are set as follows: 1 for lysis, 2 for collect...
  • Page 25 Note: Click “Save” to save the current step setting parameters. Option settings Select the ( ) icon on the top right of the protocol step interface to enter the options setting interface. Fig. 28 Option settings Click “save” to keep the adjusted parameters (Fig. 31). Table 4 Option Settings Legend Step Option...

  • Page 26: Create New Protocol Interface

    settings tip combs will pause at position is on the the top of the plate. top of the kit. After bead collection, the tip combs will pause at a Specify location specified position above the liquid surface level. Horizontal velocity (1- Set horizontal movement speed of magnetic 100mm/s) rods &...

  • Page 27: Acquire Protocol From Instrument Interface

    5. Acquire Protocol from Instrument Interface Before acquiring a protocol from the instrument onto the app, the Android device must be connected to the instrument via Bluetooth. After establishing a Bluetooth connection, the app allows viewing and downloading the saved protocols and the running logs from the instrument.
  • Page 28 Fig. 31 displays the current protocols saved in the instrument’s internal memory. Users can select protocols to download onto the app (Fig. 32). Fig. 32 Download protocol from instrument to app Running log Select the “Running log” tab and a log to download onto the app. Click the “Download running log”...

  • Page 29: Instrument Bluetooth Connection Interface

    When the Bluetooth is turned on for the Android device, the app can directly open the “Instrument Bluetooth Connection” interface. The “My instruments” section displays each IsoPure Mini the Android device has connected to. The right side of the screen will indicate which instrument the app is currently connected to.
  • Page 30 Modify instrument information In the “Instrument Bluetooth Connection” interface. Select the “ ” icon to rename the instrument’s Bluetooth ID or to disconnect the Bluetooth connection. Fig. 37 Modify instrument Fig. 38 Instrument Bluetooth information information Click "Disconnect" to disconnect the Bluetooth connection between the Android device and the instrument.

  • Page 31: Instrument Software Interface

    7. Instrument software Start-up Interface Upon powering on the instrument, the start-up screen will be displayed (Fig. 39). Fig. 39 Start-up Screen Run Interface Select “Run” to enter the Run interface. This interface is divided into two views: Shortcut View and List View. (Fig. 40, Fig. 41). Press the icons: to toggle between views.
  • Page 32 Click the “ ” icon to preview the selected protocol, as shown in figure 43 below. Fig. 42 Preview protocol Table 5. Run Interface Icons Legend Icon Definition Delete the selected protocols from the instrument’s internal memory. Switch from Shortcut view to List view, and vice versa. Activate the instrument scanning software, which allows the scanning of a QR code for instant protocol upload.
  • Page 33 Running a protocol Click the icon to start a protocol run, and icon to pause. (Fig. 43). During a protocol run, a progress bar at the bottom of the screen provides an indication of the process. Remaining time, temperature of the heating blocks, current step name and details of the current step are also displayed.
  • Page 34 Table 6. Log Icons Legend Icon Definition Delete the selected logs Search for a specific log Export log to a USB flash drive Settings interface The settings interface has 4 tabs: “UV”, “Fan”, “System” and “Maintenance”. UV: The time of sterilization can be set. Click the start button to begin the process. The UV light will turn off automatically after the timer has ended.
  • Page 35 System: Settings for “System” interface is divided into “Time”, “Language”, “Transfer”, “Bluetooth” and “Version”, as shown in figure 48 and 49. System Maintenance Fig 47. System settings System Maintenance Fig 48. System settings page 2 Time: Change the currently displayed time on the instrument. Fig 49.
  • Page 36 “items”. Then, select the "Import " (or “Export”) button to enter the flash drive directory. Select the required protocols for transfer and press "Ok" to import/export. Bluetooth: Turn Bluetooth on/off; the Bluetooth ID is the serial number of the IsoPure Mini. Fig 51. Bluetooth Setting...
  • Page 37 Select the icon to turn the Internal LED lamp on/off (Fig. 52). Fig 52. Turn internal LED lighting on/off Maintenance: Please contact Accuris Instruments with regards to maintenance of the instrument. The instrument is calibrated prior to shipping to ensure proper function.

  • Page 38: Chapter 6 Troubleshooting

    Replace switch No display after Replace fuse (250V F6.3A turning on power Fuse failure 5X20) Other Contact Accuris Instruments Replace UV bulb No UV light UV bulb failure Contact Accuris Instruments Replace LED No internal light LED Light failure Contact Accuris Instruments...
  • Page 39 Software Error Codes Fault Error Code Overheating of Heating block positions 1, 6, 7, & E011, E021, E031, E041 12 (lysis & elution wells) Open circuit in positions 1, 6, 7, & 12 (lysis & E015, E025, E035, E045 elution wells) Short circuit in positions 1, 6, 7, &...
  • Page 40 Memo...

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