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[ CARE AND USE MANUAL ]
II. GETTING STARTED
Each Anionic Polar Pesticide Column comes with a Certificate
of Analysis and a Performance Test Chromatogram. The
Certificate of Analysis is specific to each batch of packing
material and includes the gel batch number, physical
characterization, analysis of unbonded particle, analysis of
bonded particles, and a UPLC chromatographic batch test.
The Performance Test Chromatogram is specific to each
batch and contains the following information: gel batch
number, column serial number, USP plate count, USP tailing
factor, capacity factor, and chromatographic conditions under
normal-phase LC conditions. These data should be stored for
future reference, in accordance with laboratory requirements.
a. VanGuard Pre-columns
VanGuard Pre-columns are 2.1 mm I.D. x 5 mm length guard
column devices designed specifically for use on the ACQUITY
UPLC Systems. VanGuard Pre-columns are packed with the
same chemistries and frits as the 2.1 mm I.D. Anionic Polar
Pesticide columns. VanGuard Pre-columns are designed
to be attached directly to the inlet side of Anionic Polar
Pesticide Column.
Note: In order to ensure void-free and leak-free connections, the
VanGuard Pre-column is shipped with the collet and ferrule NOT
permanently attached. Care must be taken when removing the
O-ring that holds these two pieces on the pre-column tubing.
ACQUITY UPLC Column
Place wrench here
Ferrule
Flow
Figure 1. Connecting a VanGuard Pre-column to a column.
Installation Instructions
1. Remove VanGuard Pre-column from the box and shipping
tube and then remove plastic plug.
2. Orient the pre-column so that the male end is facing up
and carefully remove the rubber O-ring that holds collet
and ferrule in place during shipping (collet and ferrule are
not yet permanently attached).
VanGuard Pre-column
Collet
Place wrench here
3. Orient the Anionic Polar Pesticide Column perpendicular to
the work surface so that the column inlet is on the bottom
(facing down), with the column outlet on top (facing up).
4. From below, insert VanGuard Pre-column into Anionic
Polar Pesticide Column inlet and hand-tighten (collet
and ferrule are not yet permanently attached).
5. While pushing the the VanGuard Pre-column into the
column inlet, turn the assembled column and pre-column
180˚ so that the pre-column is now on top.
6. Tighten with two 5/16" wrenches placed onto the flats of
the Anionic Polar Pesticide Column and VanGuard Pre-
column hex nut (male end) as shown above.
7. Tighten 1/4 turn to set collet and ferrule.
8. Check that the ferrule is set by loosening the connection
and inspecting the ferrule depth. A properly set ferrule
depth will resemble other connections in the ACQUITY
UPLC Systems.
9. Reattach the pre-column by connecting the VanGuard
Pre-column with the analytical column.
b. Column Installation
(with or without a VanGuard Pre-column)
Notes: It is not recommended to run with 100% water for an
extended period of time on this column. The Anionic Polar
Pesticide Columns are shipped dry.
1. Before connecting the column (or VanGuard and
analytical column combination) to the LC system,
a. Ensure your LC and flow path are clean. For more
information, see Section IV of this manual.
b. Prime the solvent lines, to waste, with mobile phase A
(0.9 % formic acid in water) and mobile phase B (0.9 %
formic acid in acetonitrile) for at least 3 minutes.
Then connect the inlet end of the column to the injector
outlet. Arrows on the column indicate the correct direction of
solvent flow.
c. eCord Installation
The Anionic Polar Pesticide Column includes an eCord button
which can be attached to the ACQUITY UPLC Systems and
should be attached to the side of the instrument's column
heater module. The eCord button is magnetized and does
not require specific orientation.
Anionic Polar Pesticide Column
2
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