Run Timing Sequence And Reagent Consumption - General References; References - bioMerieux PREVI Color Gram User Manual

R u n t i m i n g s e q u e n c e a n d r e a g e n t c o n s u m p t i o n - G e n e r a l r e f e r e n c e s
The following table includes the general references for a staining cycle at DECOLORIZER 3.
(0)
*
R e f e r e n c e s
PREVI™Color Gram CD Rom
PREVI™Color Gram User's Manual
PREVI™Color Cytocentrifuge Rotor User's Manual
General Safety and Regulatory Information
REAGENTS
Nozzles * 2
Nozzle o-ring
Nozzle wire
Nozzle cleaning solution
Slide carousel : 12 / 30
D&E empty bottle
D&E 5-Liter empty bottle
Waste tubing
Waste container
Maintenance kit
PREVI™ Color Gram User's Manual
Phases
FIXATION*
1 CRYSTAL VIOLET
2 WASH
3 IODINE
4 WASH
5 DECOLORIZER
6 WASH
7 HIGH SPEED DRY
Total without alcohol fixation
Total with alcohol fixation
Option for ethanol or methanol fixation.
PRODUCTS
•
fr, en, de, es, it, pt, el, sv, da, pl, hu, sk
•
et, lt, lv, ru, ja, tr, na, zh, ko, th
•
Acetone safranin-A
•
Safranin-A
•
Acetone fuchsin-A
•
Fuchsin-A
•
Iodine-B
•
Crystal violet-C
12
PUMP
Time
sec
(E) (70)
C
35
D 25
B 30
D 33
A 39
D 20
- 55
237
4 min
307
5.1 min

References

Slides carousel
30
Volume Time Volume
ml sec
(3.0) (82)
4.5 43
17.3 34
4.5 38
22.8 69
6.0 47
13.8 34
- 55
320
69
5.3 min
402
144
6.7 min
REFERENCES
See your
bioMérieux
representative
General
ml
(3.5)
5.5
23.5
5.7
47.8
7.2
23.5
-
113.3
116.8
2-3