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Table of Contents
- 1 Table of Contents
- 2 Sommaire
- 3 Nuclisens Easymag® Overview
- 4 How to Start the System
- 5 How to Prepare the System
- 6 How to Define Extraction Request
- 7 How to Organize Run
- 8 How to Perform Lysis on Board
- 9 How to Prepare Pre-MIX (if Applicable)
- 10 How to Prepare Sample Vessel
- 11 Tracability Options
- 12 How to Perform Extraction
- 13 How to Manage Results
- Download this manual
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Table of Contents
Related Manuals for bioMerieux NucliSENS easyMAG
Summary of Contents for bioMerieux NucliSENS easyMAG
- Page 1 MOLECULAR BIOLOGY SIMPLIFIED USER GUIDE ® NucliSENS easyMAG 2011/06/24...
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Page 2: Table Of Contents
SOMMAIRE SOMMAIRE ....................................2 NUCLISENS EASYMAG® OVERVIEW ............................3 HOW TO START THE SYSTEM ..............................3 HOW TO PREPARE THE SYSTEM ..............................4 HOW TO DEFINE EXTRACTION REQUEST ............................. 5 HOW TO ORGANIZE RUN ................................6 HOW TO PERFORM LYSIS ON BOARD ............................7 HOW TO PREPARE PRE-MIX (IF APPLICABLE) .......................... -
Page 3: Nuclisens Easymag® Overview
NucliSENS easyMAG® OVERVIEW Reagent bay Filter Work area Status led Touch screen Keyboard HOW TO START THE SYSTEM Switch on instrument, computer and touch screen If necessary, open the easyMAG software using desktop shortcut or go to Start/Program/easyMAG and select... -
Page 4: How To Prepare The System
HOW TO PREPARE THE SYSTEM Check all status Error Warning 1. Instrument, NucliSENtral and Disposables 2. Devices 3. Incidents & details 4. Instrument status Go to section F See possible actions below Warning Error* * In case of error run cannot start 5. -
Page 5: How To Define Extraction Request
HOW TO DEFINE EXTRACTION REQUEST Define extraction request 1. Define Matrix, Protocol, input Volume (mL), Eluate volume (µL), type of sample (lysed or non- lysed=Primary) 2. Then enter Sample ID (manually or using barcode) 3. Enter lysis buffer lot number (Optional) in case of lysed sample 4. -
Page 6: How To Organize Run
HOW TO ORGANIZE RUN Identify and Organize run Create new run Enter run name Define lysis workflow On-board or Off-board Define silica incubation workflow On-board or Off-board* Off-board possible only with Generic protocol Sample addition guidance Click on OK Transfer sample ID into run Select appropriate run Select sample(s) ID you want to Transfer selected sample(s) ID... -
Page 7: How To Perform Lysis On Board
HOW TO PERFORM LYSIS ON BOARD Add primary sample to vessel 2 scenarios On the bench On the instrument Using the sample vessel carrier 1. Load sample in the vessel 1. Load sample vessel on the instrument 2. Scan position then sample vessel barcode number 2. -
Page 8: How To Prepare Pre-Mix (If Applicable)
® NucliSENS easyMAG multi-pipet 1. Dilute Internal Control in diluent using program 1 ® 2. Add NucliSENS easyMAG Silica using program 1 3. Dispense premix into micro well strip using program 2 HOW TO PREPARE SAMPLE VESSEL For lysed sample on-board:... -
Page 9: Tracability Options
Add prepared pre-mix to sample vessel and mix using program 3 of the ® NucliSENS easyMAG multi-pipet TRACABILITY OPTIONS Barcode input Possible to identify lot number for following items: Internal Control (manually) Silica (scan barcode or manually) Diluent (manually) Assign reagent lot number (if identified) to sample ID 1. -
Page 10: How To Perform Extraction
HOW TO PERFORM EXTRACTION Check all status 1. System 2. Disposables 3. Reagent inventory 4. Waste bottle Empty waste bottle if necessary Replace / Identify reagent bottle if necessary Start run... - Page 11 Possible to follow run progress 1. Remaining time 2. Step in progress Capture Wash Eluate Finish 3. Possible action Add remark to selected request Abort run Check finished process before opening cover 1. Last step finished 2. Run finished 3. Instrument idle Collect eluates within 30 minutes Perform amplification/detection process or store eluates according to specifications...
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Page 12: How To Manage Results
HOW TO MANAGE RESULTS View results 1. Select unassessed run 2. Check reported information Result Incidents Assigned kit On-board layout reagents reagents Repeat extraction if necessary 3. View results layout 4. Select request(s) to repeat extraction 5. Initiate repeat extraction Assess run 1.
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