Operating The Previ™ Color Gram Clean Reprime Stage - bioMerieux PREVI Color Gram User Manual

Operating the PREVI™ Color Gram
CLEAN reprime stage
CAUTION!
IMPORTANT!
WARNING!
C L E A N r e p r i m e s t a g e
IMPORTANT!
Switch on the
instrument.
Open the lid and insert
an empty carousel
matching the proper
position.
Close the lid.
4-2
The reagent AND waste liquid levels must be monitored by the user.
To ensure proper staining and to protect the instrument, never allow :
- a reagent line to run dry. When the reagent liquid level is near the bottom of a
reagent bottle, replace the bottle, see "Replacing a reagent bottle", page 4-12.
- the waste liquid level to be above the maximum safety level. When the waste
container is full, empty it. See "Emptying the waste container", page 4-13.
To obtain optimum performance, thoroughly purge and prime each reagent
delivery line using the following instructions.
Never operate a dry pump for more than 10 seconds.
The CLEAN cycle is a two-stage process that re-primes reagents into the nozzles
then uses ethanol or methanol 99.8% pure to purge the reagent nozzles of
precipitates and debris.
If you have not used the PREVI™ Color Gram before, go directly to "Installing
reagent bottles", page 3-4.
This stage must be completed before restarting several runs to complete the
CLEAN cycle.
The CLEAN reprime stage requires the use of 7.5 ml of A, B, C reagents (Alcohol,
Iodine, Crystal Violet) and 10 ml of reagent D (Distilled Water) divided equally
between each nozzle.
PREVI™ Color Gram User's Manual