Chapter 5 Running A Sample; Preparing Your Sample - Thermo Scientific ISQ User Manual

Running a Sample

Preparing Your Sample

Thermo Scientific
This chapter describes how to prepare a sample and then run a sequence.
Contents
• Preparing Your Sample
• Running a Sequence
The primary goal of sample preparation is to reduce the amount of unwanted contaminant in
a sample or to increase the reliability of sample detection. As a result, you should prepare your
samples to increase the relative abundance of compounds you want to analyze and decrease
the relative abundance of the compounds you aren't interested in. Refer to your lab's standard
operating procedure (SOP) for your particular sample preparation method.
Different solvents are used to dissolve different compounds. Be sure to choose a solvent that
will dissolve the compounds you want to analyze. The solvent should be compatible with the
stationary phase of your GC column when making sample dilutions.
Once your sample is prepared, you can transfer it to a sealed vial so that you can inject it into
the GC. It is important to use a sealed vial because you do not want the concentration to be
altered, which is what happens when the solvent evaporates.
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