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6.2.5 Manual defrosting of the cryochamber
Fig. 19
6.2.6 Setting the section thickness
Fig. 20
Leica CM1520 – Cryostat
You can activate manual defrosting of the cryochamber by pressing the
key (continuous tone sounds), followed immediately by the
the cryochamber temperature selection field (continuous tone stops). The
display flashes during the defrosting (duration 12 min.).
To shut off the manual defrost early, press the
by the
button in the cryochamber temperature selection field.
Adjust the section thickness using the
control panel field (see Fig. 20) in a range from 2 - 60 µm:
From 2 - 5 µm in 0.5 µm increments
From 5 - 20 µm in 1 µm increments
From 20 - 60 µm in 5 µm increments
The preselected section thickness appears in the display above the keys.
• Start trimming at approx. 20 µm.
• Decrease the section thickness continually down to the appropriate value.
After changing the section thicknesses, discard the first two or three
sections.
• For sectioning, turn the handwheel at a consistent speed.
6.
Operating the Instrument
key, followed immediately
and
pushbuttons in the top
key in
29
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