Review A Stored Spectrum; Subtract A Spectrum - Waters 2489 Operator's Manual

Uv/visible detector
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Review a stored spectrum

Once you store a spectrum, you can retrieve it for review from one of the three
available storage slots by selecting the Review option from the Scan choice
list.
To review a spectrum:
1.
Press SCAN (Shift Chart-mark) to view the Scan choice list.
2.
Press 4, Review.
When you select Review, you actually are retrieving the zero scan
Tip:
and the sample scan that were stored as a pair.
3.
Enter the storage slot number (1 to 3) of the spectrum you want to
review.
4.
Press Enter. The message "Retrieving spectrum n" appears.
Once you retrieve a spectrum for review, you can view it graphically and
adjust the wavelength and AU ranges, if necessary. You can also run a new
sample scan based on the retrieved zero scan.

Subtract a spectrum

Once you store more than one spectrum, you can create a difference spectrum.
The current spectrum is the spectrum that the stored spectrum (whose
Tip:
slot number you enter) is subtracted from.
To subtract and review the difference spectrum:
To subtract the stored spectrum from the current spectrum, the starting
Rule:
and ending wavelengths (λ1 and λ2) and the pace of both spectra must be
identical.
1.
Press SCAN (Shift Chart-mark).
2.
Press 5, Subtract & review.
3.
Enter the storage slot number (1 to 3) of the spectrum you want to
subtract out from the current (or retrieved) spectrum.
4.
Press Enter.
Once the difference spectrum appears, you can store the results in one of the
three storage slots.
November 9, 2012, 715003800 Rev. A
Scan spectra
109

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