Thermo Scientific Sorvall WX+ Instruction Manual
  1. Manuals
  2. Brands
  3. Thermo Scientific Manuals
  4. Laboratory Equipment
  5. Sorvall WX+
  6. Instruction manual

Thermo Scientific Sorvall WX+ Instruction Manual

Ultracentrifuge
Hide thumbs
Table of Contents

Advertisement

Quick Links

Thermo Scientific
Sorvall WX+ Ultracentrifuge
Instruction Manual
50145792-a
•
09 / 2014

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Sorvall WX+ and is the answer not in the manual?

Questions and answers

Related Manuals for Thermo Scientific Sorvall WX+

Summary of Contents for Thermo Scientific Sorvall WX+

  • Page 1 Thermo Scientific Sorvall WX+ Ultracentrifuge Instruction Manual 50145792-a • 09 / 2014...
  • Page 2 General description The Thermo Scientific™ Sorvall™ WX+ Ultracentrifuge is designed to separate liquid-suspended materials having different densities and particle size. The series is a series of products that pursue user-friendliness and reliability based on our many years of experience in developing centrifuges. This series offers many new features that we are confident of satisfying your requirements.
  • Page 3 SAFETY NOTICES Safety reminders Carefully read and fully understand the following safety instructions. Operate your instrument according to the instruction manual. ● Be sure to observe the all safety precautions in the instruction manual and safety ● instructions on your instrument. If neglected, personal injury and/or instrument damage can be caused.
  • Page 4 SAFETY NOTICES ○ Mechanical Safety Do not open the door while the rotor is spinning. ● WARNING： Do not attempt to slow or stop the spinning rotor by hand. ● Do not incline or move the instrument while the rotor is spinning. Do not ●...
  • Page 5 SAFETY NOTICES CAUTION： Install the rotor carefully and securely on the drive shaft (crown) in the rotor ● chamber. Always place the rotor pin in the drive hole (crown hole) apart from the crown pin. Take care not to get your hands or fingers caught in the door of the ●...
  • Page 6 SAFETY NOTICES CAUTION： If the centrifuge is exposed to ultraviolet rays for a long time, the color of the ● covers may be changed or the coating may be peeled off. After use, cover the centrifuge with a cloth to protect it from direct exposure. ○...
  • Page 7 SAFETY NOTICES Precaution indications in this Manual The followings informations indicate the precaution indications and the chapters/sections which mention them in this manual. 1.Indication of DANGER To avoid electrical shock hazards, follow below when servicing the centrifuge. 1) Be sure to turn off the POWER switch and turn off the distribution board of your centrifuge room if your centrifuge is equipped with a three wire power cord.
  • Page 8 Thermo Fisher Scientific may ask you about the treatment for the centrifuge, rotor or the part if the decontamination is checked and judged as insufficient by Thermo Scientific. It is your responsibility to bear the cost of sterilization or decontamination.
  • Page 9 SAFETY NOTICES Zonal centrifugation includes operations where the rotor will turn while the door is open. Be sure to read though the “Zonal rotor instruction manual” before operation. (Section 3-4-3) The cap is installed by hand on the rotor when it is turning. Perform this operation with care and be sure to follow the instructions in the instruction manual.
  • Page 10 SAFETY NOTICES WARNING Never u se any material capable of producing flammable or explosive vapors. Be sure to take the necessa ry precautions before sepa ra ting radio ac tive, to xic o r pa thog enic materials. AVERTISSEMENT Ne jamais utiliser de matériau capable de produire des vapeurs inflammables ou explosives.

  • Page 11: Table Of Contents

    Table of Contents 1. Specifications ······························································································ 1-1 2. Description ·································································································· 2-1 2-1 External View of Ultracentrifuge ································································· 2-1 2-2 Structure ······························································································· 2-2 ··················································· 2-2 2-2-1 Touchscreen and External Connection ················································································ 2-6 2-2-2 Rotor Chamber ················································································ 2-7 2-2-3 Safety Devices ······································································· 2-8 2-2-4 Rotor overspeed decal 3 .
  • Page 12 Table of Contents ··············································································· 3-84 3-4-6 Admin Function ········································································ 3-85 (1) User management ··············································································· 3-90 (2) User lockout ······································································· 3-92 (3) Rotor management ······································································· 3-98 (4) Run time indication ··········································································· 3-98 (5) Actual run timer ·············································································· 3-99 (6) Vacuum level ···············································································...

  • Page 13: Specifications

    1. Specifications 1. Specifications Model Sorvall WX100 Plus Sorvall WX90 Plus Sorvall WX80 Plus Maximum speed 100,000 rpm 90,000 rpm 80,000 rpm 802,000 x g 692,100 x g 602,600 x g Maximum RCF* (T-8100) (T-890) (T-880) Speed control accuracy ±10 rpm（1,000 rpm to maximum speed） 10-stage variable acceleration control, 10-stage braked deceleration Acceleration/deceleration control, plus coasting deceleration...
  • Page 14 1. Specifications Model Sorvall WX100 Plus Sorvall WX90 Plus Sorvall WX80 Plus Width: 790 mm; Depth: 690 mm; Height: 880 mm Depth with safety cover mounted: 890 mm Dimensions Height to the handle of the centrifuge door: 925 mm Weight 390 kg Supply required Single phase 50/60 Hz...

  • Page 15: Description

    2. Description 2-1 External View of Ultracentrifuge 2. Description 2-1 External View of Ultracentrifuge The Sorvall WX+ Ultra series centrifuges are floor-standing types. These three types of ultracentrifuges have the same external view, except for the model name printed on front cover and the same dimensions.

  • Page 16: Structure

    2-2 Structure 2-2-1 Touchscreen and External Connection 2-2 Structure 2-2-1 Touchscreen and External Connection The touchscreen with color liquid crystal screen is incorporated in the Sorvall WX+ Ultra series centrifuges. You can set the run parameters, perform the operation, and display a run history, programmed operation, and user customizations screens by pressing the screen.
  • Page 17 2-2-1 Touchscreen and External Connection 〔Functions of the Run screen〕 Name and symbol Functions and actions These areas display various types of information. Display areas The SPEED (RCF), TIME, and TEMP areas display the current status in the upper part and the specified setting in the lower part.
  • Page 18 2-2-1 Touchscreen and External Connection 〔Functions of the Run screen〕 Functions and actions Name and symbol The following four stages are displayed, depending on the vacuum of the ● rotor chamber. Atmospheric state. （1） The vacuum pump is not active. Low vacuum.
  • Page 19 2-2-1 Touchscreen and External Connection 〔Functions of the Run screen〕 Functions and actions Name and symbol On-screen keypad Use the on-screen keypad to enter numeric values for run ● parameters. [ : / . ] When entering a time: Switches from hours to minutes [Hold] When entering the operation time: Sets continuous running.

  • Page 20: Rotor Chamber

    2-2-2 Rotor Chamber 2-2-2 Rotor Chamber The structure of the rotor chamber (vacuum chamber) is shown in Fig. 2-2-2. Bowl (Rotor Chamber) Drive shaft (crown) Handle Chamber door seal Chamber door Speed sensor Overspeed detector Door lock system (detecting any instance exceeding the maximum allowable speed of the rotor) Vacuum chamber...

  • Page 21: Safety Devices

    2-2-3 Safety Devices 2-2-3 Safety Devices (1) Protection of rotor chamber Should the rotor spinning at high speed fails (or comes off the drive shaft), the safety of the operator is ensured by the thick protective steel ring enclosing the bowl (Fig. 2-2-2). (2) Imbalance detector If during operation the vibration of the rotor becomes excessive due to serious imbalance or improper bucket setting, the imbalance detector detects the situation and decelerates the rotor immediately.

  • Page 22: Rotor Overspeed Decal

    2-2-4 Rotor overspeed decal 2-2-4 Rotor overspeed decal Overspeed decal The overspeed decal located on the rotor base has alternating black and white bands. The number of bands corresponds to the maximum permitted speed of the rotor. (See Fig. 2-2-3.) Overspeed decal Fig.2-2-3 Standard Rotor To protect the overspeed decal, be sure to store the rotor on the rotor stand provided.
  • Page 23 2-2-4 Rotor overspeed decal WARNING: The overspeed decal must match the maximum speed of the rotor, because the overspeed decal is a critical component that detects the over-speed of rotor: If an overspeed decal that is not compatible with the rotor is attached, the rotor could break, resulting in damage to the ultracentrifuge.

  • Page 24: Operation

    3. Operation 3. Operation The centrifuge comes with a wide range of functions to support a broad range of applications. A brief description of each mode of operation is given below. Brief description Reference Section 3-2 "Basic Operation" Normal operation Time You can save set run parameters in memory for later use in repeated operation.
  • Page 25 3. Operation Brief description Reference This function is used to perform a run between a set start time and end time. Section 3-3-4 "RTC End of set Operation" Press the run time RTC operation START （Real Time Control） button Time [Special operation] Brief description Reference...

  • Page 26: Run Preparation

    3-1 Run Preparation 3-1-1 Starting up the centrifuge 3-1 Run Preparation WARNING: (1) This centrifuge is not explosion proof centrifuge. Never use explosive or flammable samples or materials that chemically react vigorously. Do not centrifuge such materials in this instrument nor handle or store them near the instrument.

  • Page 27: Rotor And Tubes Preparations And Precautions

    3-1-2 Rotor and Tube Preparations and Precautions 3-1-2 Rotor and Tube Preparations and Precautions The Sorvall WX+ Ultra series centrifuges allow you to balance samples, by eye. The difference between meniscus levels of opposing samples must be less than 5 mm (Fig. 3-1-2), but some rotors are exceptions.

  • Page 28: Basic Operation

    3-2 Basic Operation 3-2-1 Setting Run Parameters 3-2 Basic Operation WARNING: (1) Do not incline or move the instrument while the rotor is spinning. Do not place any object on the instrument or lean on the instrument. (2) The centrifuge itself may move if the rotor fails during high-speed rotation. Make sure there is a 30 cm area around the centrifuge that will allow for such movement and do not allow individuals to enter into that area during operation.
  • Page 29 3-2-1 Setting Run Parameters [Display and operations when entering run parameters] Press the SPEED, TIME, TEMP, ACCEL or DECEL field on the touchscreen to display the on-screen keypad. (1) Press the field of the item you want to set. The initial digits are displayed in blue.
  • Page 30 3-2-1 Setting Run Parameters Methods for setting run parameters are described on the next page. Note 1) If you enter the wrong value, press the [CE] button and enter the correct value. If you have already pressed the [Enter] button, repeat the process and enter the correct value.
  • Page 31 3-2-1 Setting Run Parameters  Setting speed, run time and temperature Operation procedures will be described using examples. Speed run time Temperature Setting Item （SPEED） （TIME） （TEMP） Setting example 100,000 rpm 2 hours 30 minutes °C Press the SPEED, TIME or TEMP field to display the on-screen keypad.
  • Page 32 3-2-1 Setting Run Parameters  Setting acceleration and deceleration mode Acceleration Deceleration Setting Item （ACCEL） （DECEL） Setting example Press a field to display the on-screen keypad. Press the field whose value you want to set. The numeric values are displayed in blue. Color: Blue Color: Blue Press the on-screen...

  • Page 33: Acceleration And Deceleration Modes

    3-2-2 Acceleration and Deceleration Modes 3-2-2 Acceleration and Deceleration Modes In order to meet a wide variety of applications, the acceleration and deceleration rates can be adjusted between 0 to 500 rpm. By setting an ideal acceleration and deceleration time samples will not be disturbed, which improves efficiency and reduces centrifugation time.

  • Page 34: Rotor Selection

    3-2-3 Rotor Selection 3-2-3 Rotor Selection This centrifuge stores the maximum and average radius of each rotor in internal memory. It provides a function that will automatically calculate and display the relative centrifugal force (RCF) from a set speed and calculate and display the speed from an RCF setting (for information, refer to section 3-3-2 "RCF display and setting function").
  • Page 35 3-2-3 Rotor Selection Step Touchscreen operation Screen displays and notes When the Rotor management The ROTOR ● screen does not display the CATALOG screen desired rotor, press the [Rotor Catalog] button. In the ROTOR CATALOG screen, select the desired rotor type. In the screen displaying rotor The screen for ●...
  • Page 36 3-2-3 Rotor Selection Step Touchscreen operation Screen displays and notes The Run screen appears and The Run screen ● appears. the name of the selected rotor type is displayed in the Rotor indicator field. Rotor indicator field NOTE (1) You cannot perform life management of a rotor just by selecting a rotor in the ROTOR CATALOG screen.

  • Page 37: Logging In As A User

    3-2-4 Logging in as a User 3-2-4 Logging in as a User By registering users in the instrument, it will be possible to manage the operation history of users that log in. For information on how to register a user, refer to Section 3-4-6 (1) "User Management." User Login Procedures Step Touchscreen operation...
  • Page 38 3-2-4 Logging in as a User Step Touchscreen operation Screen displays and notes The Run screen The user name appears in the ● appears. USER area in the Run screen. USER area NOTE 1) When the user lockout function described in Section 3-4-4 (2) "User Lockout" is enabled, you must login as a user to operate the centrifuge.

  • Page 39: Operation Procedures

    3-2-5 Operation Procedures 3-2-5 Operation Procedures Below is a description of normal operating procedures. CAUTION: Take care not to get your hands or fingers caught in the door of the centrifuge. Before you start using your centrifuge, carefully read through the instruction manual for your NOTE rotor and make sure that the correct tube types and number of samples are supplied.
  • Page 40 3-2-5 Operation Procedures Step Touchscreen operation Centrifuge operations and notes The [START] button lamp blinks and the rotor starts turning. Press the [START] button.  The timer begins operating. (If the actual run timer is set, the  timer will start operating when set speed is reached.) When the set speed is reached, the [START] button lamp ...
  • Page 41 3-2-5 Operation Procedures Fig. 3-2-3 is an overview of the procedures described above. See Section 3-4-3 "Zonal Operation Procedures" for information on using a zonal rotor. Start of operation Turn on the : Indicates switch or button. centrifuge POWER Open the door and install the rotor.

  • Page 42: How To Use The Function Selection Area

    3-3 How to Use the Function Selection Area 3-3 How to Use the Function Selection Area This centrifuge provides programmed operation including step-mode operation, RCF display and setting function, ω T display and setting function and Real Time Control (RTC) that can run the centrifuge at a preset date and time and a host of other functions.

  • Page 43: Programmed Operation

    3-3-1 Programmed Operation 3-3-1 Programmed Operation When a centrifugal condition will be used frequently, entering the same condition every time you want to perform centrifugation is inconvenient. This centrifuge has a programmed operation feature that stores run parameters. Storing frequently used run parameters allows you to call those parameters when you need them, which saves setup time.
  • Page 44 3-3-1 Programmed Operation (Basic operation of the programmed operation feature) Press the [PROGRAM] button. Program Step operation display display screen The Function Selection Area changes to the Program display screen. [PROGRAM No.] button [Folder] button If the registered program If the registered program No.
  • Page 45 3-3-1 Programmed Operation As the Run screen appears, ● the registered run parameters are set. program No. is displayed ● in the [Folder] button in the Program display screen. When multiple steps are ● registered, the step operation display indicates the total number of steps and the current step.

  • Page 46: Program Registration Procedures

    3-3-1-1 Program Registration Procedures 3-3-1-1 Program Registration Procedures (1) Procedures for registration of run parameters Procedures for registering run parameters are described below. Step Touchscreen operation Screen displays and notes Turn on the centrifuge The Run screen ● appears. POWER switch. [PROGRAM] button Press the [Program] button The Function Selection...
  • Page 47 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes Press the [Folder] button in The Run screen ● replaces the the program display screen. Program list screen. Program folders ● come in four colors. The program list can ●...
  • Page 48 3-3-1-1Program Registration Procedures Step Touchscreen operation Screen displays and notes Press the [New Folder] The Program No. ● entry screen appears. button. Program No. entry field The unregistered To register the folder, press program with the the [Enter] button. To select lowest number is a different number, use the displayed when the...
  • Page 49 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes Press the white area in the The User Management ● Registered user screen appears. display/registration field ((2)) to select a user. (You can omit this operation.) Select the user name you want to use and press the ...
  • Page 50 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes Press the field in Step 1, check that the field turns blue and press the [Edit] button. [Edit] button run parameter ● Press the white area in the entry/display screen SPEED setting value display appears.
  • Page 51 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes The run parameter Check the entry and press ● entry/display screen the [OK] button. reappears. The SPEED, TIME, ● TEMP display fields and the on-screen keypad appear. Selecting a rotor in step ●...
  • Page 52 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes The RCF setting screen Press the display area of the ● appears. item you want to set and Set rotor model enter the desired value. Example) SPEED display RCFmax：513,700 x g RCFavg setting value display field RCFmax setting value...
  • Page 53 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes setting ω Press the ω T display area ● screen appears. and enter the desired value. Example) ω T：1.98×10 display ω (Left area) Mantissa (Right area) Exponent Enter the desired value and press the [Enter] button. ...
  • Page 54 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes Check the setting and press When a normal operation is registered the [Register] button. The Program list ● screen appears. Program registered in this example When a step-mode operation is registered The Check registered ●...
  • Page 55 3-3-1-1 Program Registration Procedures (2) Procedures for Changing Program run parameters The following describes how to change run parameters for registered programs. Step Touchscreen operation Screen displays and notes Press the [PROGRAM] button in the Run screen (in the Function Selection Area). [Folder] button [PROGRAM] button Program display screen...
  • Page 56 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes The run parameter To change run parameters, ● setting/display screen press the [Next] button. appears. [Edit] button Press a field in Step 1, check The run parameter ● entry/display screen that the field turns blue and appears.
  • Page 57 3-3-1-1 Program Registration Procedures (3) Procedure for deleting programs The following describes how to delete registered programs. Step Touchscreen operation Screen displays and notes Open the Program list The program list ● appears and the screen and press the frame of the selected program folder you want to folder turns blue.
  • Page 58 3-3-1-1 Program Registration Procedures (4) Operation procedures for copying programs The following describes how to copy a registered program and register the result as a new program. This is a convenient method when you want to register a new program by changing part of a registered program.
  • Page 59 3-3-1-1 Program Registration Procedures Step Touchscreen operation Screen displays and notes The Program list Press the [Enter] button. ● screen appears. (The [Enter] button will not accept the entry of an already registered program No.) The copied and newly registered program folder The copied program is registered under a new number.

  • Page 60: Programmed Operation Procedures

    3-3-1-2 Programmed Operation Procedures 3-3-1-2 Programmed Operation Procedures The following describes procedures for "programmed operations" by calling registered run parameters. This is convenient for using the same run parameters repeatedly. (1) If the registered program no. is known Step Touchscreen operation Screen displays and notes Turn on the centrifuge The Run screen appears.
  • Page 61 3-3-1-2 Programmed Operation Procedures Step Touchscreen operation Screen displays and notes The number of the called program is displayed in the  Program display screen. It allows you to set and display run parameters (SPEED,  TIME, TEMP, ACEL/DECEL, ROTOR, USER). The Program display screen displays the following data.
  • Page 62 3-3-1-2 Programmed Operation Procedures (2) If the registered program no. is not known Step Touchscreen operation Screen displays and notes Turn on the centrifuge The Run screen appears. POWER switch. The Function Press the [PROGRAM] ● Selection Area button in the Function changes to the Selection Area.
  • Page 63 3-3-1-2 Programmed Operation Procedures Step Touchscreen operation Screen displays and notes The MENU box appears b-1) To confirm registered ● in the Program list data, press the [Folder] screen. button you want to verify, and press the [MENU] button after the folder Reference button turns blue.
  • Page 64 3-3-1-3 Step-Mode Operation Procedures 3-3-1-3 Step-Mode Operation Procedures This centrifuge is provided with a "step-mode operation" function that can store two or more run parameters in one program memory area and a total of 30 steps. This allows it to change speed, run time, temperature and other conditions during operation.
  • Page 65 3-3-1-3 Step-Mode Operation Procedures Step Touchscreen operation Screen displays and notes As described in steps 1 to 8  Program registration screen  Run parameter setting/display in "(1) Procedures for registration of run parameters" in Section 3-3-1-1 "Program registration procedures," enter the Program No., registered rotor, user and remarks, select a folder color...
  • Page 66 3-3-1-3 Step-Mode Operation Procedures Step Touchscreen operation Screen displays and notes The run parameter Press the [Next Step] button ● entry/display screen and enter the run in STEP 3 appears. parameters for step 3 from the run parameter entry/display screen for step STEP 2 [Register] button SPEED: 60,000 rpm...

  • Page 67: Step-Mode Operation Procedures

    3-3-1-3 Step-Mode Operation Procedures (2) Step-Mode Operation Procedures Call the number of the program where the step-mode operation is registered and run it according to the instructions in Section 3-2-5 "Operation Procedures." Start operation without changing the run parameters after calling the program. (Changing the run parameters will cancel the called program.) NOTE 1) The Program display screen in the Run screen displays the following data.
  • Page 68 3-3-1-3 Step-Mode Operation Procedures (3) Procedures for Registering Step-Mode Operation that Includes Stops Follow the instructions in "(1) Registration Procedures for Step-Mode Operation." Perform the registration procedures described below. [Setting example] The following describes the setup procedures for continuous operation under the five run parameters including a 0 rpm settings shown in the figure below.
  • Page 69 3-3-1-3 Step-Mode Operation Procedures Step Touchscreen operation Screen displays and notes Enter the run parameters for The run parameter ● step 1 as described in "(1) entry/display screen Registration Procedures for appears. Step-Mode Runs." [Next Step] button STEP 1 SPEED: 50,000 rpm TIME ：1 hour TEMP ：15.0 °C ACCEL: 9...
  • Page 70 3-3-1-3 Step-Mode Operation Procedures Step Touchscreen operation Screen displays and notes The Program list Check the entries displayed ● screen reappears. for each step in the run parameter setting/display [X] button screen. If they are correct, press the [X] button and press the [X] button in the The program Program registration screen.
  • Page 71 3-3-1-3 Step-Mode Operation Procedures Step Touchscreen operation Screen displays and notes The Program display When run time for step 1 has ● screen in the Run elapsed, processing proceeds screen proceeds to to step 2 and the rotor step 2. decelerates and stops.

  • Page 72: Rcf (Relative Centrifugal Force) Display And Setting Function

    3-3-2 RCF Display and Setting Function 3-3-2 RCF (Relative Centrifugal Force) Display and Setting Function This centrifuge stores the maximum and average radii of each rotor in internal memory. Setting a speed causes the centrifuge to automatically calculate and display the RCFmax value (the maximum centrifugal force of each rotor) and the RCFavg value (the average centrifugal force of each rotor).
  • Page 73 3-3-2 RCF Display and Setting Function Step Touchscreen operation Screen displays and notes Set rotor model Press the RCF display screen The RCF setting field. ● screen appears. SPEED display Calculated RCFavg value Calculated RCFmax value The RCF setting screen displays the RCFmax and ...
  • Page 74 3-3-2 RCF Display and Setting Function Step Touchscreen operation Screen displays and notes The entered value Check the entered value and ● is confirmed. press the [Enter] button. SPEED display Calculates and displays the RCFavg value from the  entered RCFmax and the rotor set in step 1 (the value here is 355,000 x g).

  • Page 75: Ω 2 T Operation

    3-3-3 ω T Operation 3-3-3 ω T Operation This function is used to indicate ω T, (a value obtained by adding run time to the angular velocity squared) the centrifugal effect. When the same rotor is used, setting the ω T makes it possible to produce centrifugation that is easily reproducible.
  • Page 76 3-3-3 ω T Operation Touchscreen operation Screen displays and notes Step Press the on-screen keypad to enter a setting value. When the mantissa has been entered, press the exponent entry field, enter the Exponent exponent and press the entry field [Enter] button.

  • Page 77: Rtc (Real-Time Control) Operation

    3-3-4 RTC (Real Time Control) Operation 3-3-4 RTC (Real Time Control) Operation This centrifuge contains an internal clock allowing you to operate the centrifuge between a set start time and end time. This function for operating the centrifuge between set time intervals is called Real Time Control (RTC).
  • Page 78 3-3-4 RTC (Real Time Control) Operation Step Touchscreen operation Screen displays and notes The setting value is Set run parameters according ● displayed in the to the instructions in Section setting value display 3-2-1 Setting run parameters. field. Setting value display field [RTC] button When setting run parameters, do not set HOLD but enter a...
  • Page 79 3-3-4 RTC (Real Time Control) Operation Step Touchscreen operation Screen displays and notes To set the start time, press the [Start Time] button and to set the stop time, press the [Stop Time] button. Enter the Set state display start time and stop time from the on-screen keypad.
  • Page 80 3-3-4 RTC (Real Time Control) Operation NOTE 1) In the following situations RTC cannot be set. (1) When TIME (run time) is set to HOLD (continuous operation) in the Run screen Change the run time (centrifugation time) to a numeric value instead of HOLD.

  • Page 81: Features Of The Menu Screen

    3-4 Features of the MENU Screen 3-4 Features of the MENU Screen These features have been designed to make your Sorvall WX+ Ultra series centrifuge easier to use by including, for example, the Run History function shown in Fig. 3-4. The MENU screen can be displayed by pressing the MENU tab on the touchscreen.
  • Page 82 3-4 Features of the MENU Screen Name Icon Function Starts the Defrost function that heats the rotor chamber to quickly Defrost remove frost and moisture. (For details, refer to Section 3-4-4 function "Defrost ") Allows you to adjust screen displays, the stop signal and other Customize settings in a way that works best for you.
  • Page 83 3-4-1 Outputting and Reusing Run History Display and Run Parameters 3-4-1 Outputting and Reusing Run History Display and Run Parameters The centrifuge can automatically store a run history containing up to 5,120 run parameters. The run history can be used for checking operation and for reuse, verify user and rotor performance and output data in the CSV format.
  • Page 84 3-4-1 Outputting and Reusing Run History Display and Run Parameters Step Touchscreen operation Screen displays and notes The touchscreen displays the Run screen again and the Press the [Load for a new  run parameters in the history are set. run] to use the run parameters in the Run history details screen.
  • Page 85 3-4-1 Outputting and Reusing Run History Display and Run Parameters (2)-2 Checking and Using User Run History Step Touchscreen operation Screen displays and notes The Menu box in Run Press the [Menu] button at the ● History appears bottom left in the Run History screen.
  • Page 86 3-4-1 Outputting and Reusing Run History Display and Run Parameters (2)-3 Checking and Using Rotor Run History Step Touchscreen operation Screen displays and notes The Menu box in Run Press the [Menu] button at the ● History appears bottom left in the Run History screen.

  • Page 87: Rotor Catalog

    3-4-2 Rotor Catalog 3-4-2 Rotor Catalog This catalog provides information on rotor models and rotor specifications. (1) Example showing how to referencing the catalog Step Touchscreen operation Screen displays and notes Press the [Rotor Catalog] The ROTOR ● CATALOG screen button in the MENU screen.
  • Page 88 3-4-2 Rotor Catalog Step Touchscreen operation Screen displays and notes The Touchscreen again displays the previous screen, the Use the [X] button to return to  MENU screen or the Run screen. the previous page. Use the MENU tab to return to the previous MENU screen.

  • Page 89: Zonal Operation Procedures

    3-4-3 Zonal Operation Procedures 3-4-3 Zonal Operation Procedures Zonal operation uses a zonal rotor for density gradient centrifugation to efficiently process large-volume samples. Zonal operation involves the following three zonal modes. (1) With the door opened the rotor turns at * Zonal speed low-speed (* zonal speed) to allow loading of samples.
  • Page 90 3-4-3 Zonal Operation Procedures Step Touchscreen operation Instrument operations and notes Press the [ZONAL] button in The Set Validation ● the ZONAL setting screen. No. screen appears. ZONAL operation is Enter “30” for the PIN on the ● enabled when the on-screen keypad and press [ZONAL] button is the [Enter] button.
  • Page 91 3-4-3 Zonal Operation Procedures Step Touchscreen operation Instrument operations and notes The selected rotor Press the Rotor indicator field ● type appears in the to select a zonal rotor as Rotor indicator field. described in Section 3-2-3 "Rotor Selection." Rotor indicator field The rotor accelerates to zonal speed (normally 3,000 rpm) Press the [START] button.
  • Page 92 3-4-3 Zonal Operation Procedures Step Touchscreen operation Instrument operations and notes The set time elapsed. (Time When the set time elapses, the rotor decelerates and  out) stabilizes at the zonal speed. The zonal operation chart uses a steady or blinking red light ...

  • Page 93: Defrost

    3-4-4 Defrost 3-4-4 Defrost The presence of frost or moisture in the rotor chamber (especially in the bowl) prior to a run will considerably lengthen the time it will take to reach a high vacuum level. This centrifuge comes with a defrost function that heats and evacuates the bowl to quickly remove frost and moisture.
  • Page 94 3-4-4 Defrost Reference As described below, the defrost function operates differently depending on centrifuge status when defrosting is started. Evacuation is performed simultaneously with defrosting. Enabling defrosting when the rotor is Enabling defrosting when the rotor is standing still rotating Defrosting starts when selected and Defrosting starts when rotor speed starts continues for 10 minutes.

  • Page 95: Customizing

    3-4-5 Customizing 3-4-5 Customizing This function allows you to adjust Run screen displays, the stop signal, sound volume, screen backlight brightness and other functions to your preferences. Press the [Customize] button in the MENU screen to open the CUSTOM screen with the icons for setting and changing zoom display, the stop signal and other functions shown in Fig.

  • Page 96: Zoom Display Setting

    3-4-5 Customizing Name Icon Function Use for making date and time settings. Date/Time (For details, refer to Section 3-4-5 (7)) Status display Use to set status displays (LED indicator). lamp (For details, refer to Section 3-4-5 (8)) Sets economy mode (auto standby mode). (For details, refer Economy mode to Section 3-4-5 (9)) Press the icon for the function you want to use and select from the options that appear.

  • Page 97: Sound Volume Adjustment

    3-4-5 Customizing (3) Sound volume adjustment Adjusts the sound volume of the stop signal. More green segments in the indicator bar means a larger sound volume. The stop signal does not sound when all segments in the indicator bar are black. Use the sound volume adjustment buttons as described below.

  • Page 98: Language Switch Button

    3-4-5 Customizing  Pressing when the dimming backlight mode is enabled opens the screen shown in Fig. 3-4-5 (4c) that allows you to set the time when the dimming backlight mode will be engaged. You can set a time between 1 and 180 minutes in one minute increments.
  • Page 99 3-4-5 Customizing Step Touchscreen operation Instrument operations and notes Press the cell of the user The User PIN field ● appears. whose schedule you want to register. Enter the PIN of the selected The Schedule date/time ● entry screen appears. user and press the [Enter] button.
  • Page 100 3-4-5 Customizing 2) Method for canceling centrifuge schedules Step Touchscreen operation Instrument operations and notes Press the [Schedule] button. The CUSTOM ● screen is replaced by the Schedule screen. The selected cell Press the schedule cell you ● turns blue. want to delete.

  • Page 101: Date/Time Setting

    3-4-5 Customizing Step Touchscreen operation Instrument operations and notes Enter the PIN of the user who The Schedule list ● screen appears. registered the schedule and press the [Enter] button. The selected schedule is deleted from the schedule list.  Press the CUSTOM tab, the [X] button, the Run screen tab ...

  • Page 102: Status Display (Led Indicator) Lamp Setting

    3-4-5 Customizing (8) Status display (LED indicator) lamp setting Sets the display color, brightness and lighting pattern of the status display lamp (LED indicator) beside the rotor chamber door to indicate instrument operating conditions. The display color, brightness and display pattern of the status display lamp can be set for the operating conditions described below.
  • Page 103 3-4-5 Customizing Step Touchscreen operation Instrument operations and notes Press the [Set display The Color palette ● screen appears. color] button for the operating condition you want [X] button to change. [Select display color] button Example: To change the display color for [When stopped] from blue to [SET] button white...
  • Page 104 3-4-5 Customizing 3) 2 Method for setting display patterns for the status display lamp Step Touchscreen operation Instrument operations and notes The LED indicator Press the [LED] button. ● setting screen appears. [Set pattern] button Press the [Set pattern] The Pattern palette ●...

  • Page 105: Economy Mode Setting

    3-4-5 Customizing (9) Economy mode setting When economy mode is enabled, the touchscreen backlight will automatically dim and the cooling fan will stop turning to reduce power consumption when the centrifuge has been left idle for a preset period of time. 1) Method for engaging economy mode Step Touchscreen operation...
  • Page 106 3-4-5 Customizing Step Touchscreen operation Instrument operations and notes Press the CUSTOM tab or the [X] button to return to the Press the "CUSTOM" tab, the  CUSTOM screen. [X] button, the Run screen Press the Run screen tab or MENU tab to return to the ...

  • Page 107: Admin Function

    3-4-6 Admin Function 3-4-6 Admin Function This function allows you to access administrator functions such as changing the display language. Press the [Admin] button in the MENU screen to open the ADMIN screen shown in Fig. 3-4-6. This screen provides icons for controlling functions like user management and user lockout. Screen tabs Fig.

  • Page 108: Vacuum Level

    3-4-6 Admin Function Name Icon Function Allows you to set the vacuum conditions when the centrifuge starts accelerating from vacuum standby Vacuum level state to the set speed. (For details, refer to Section 3-4-6 (6)) Zonal speed can be changed in increments of 100 rpm Zonal speed between 2,000 and 3,000 rpm.
  • Page 109 3-4-6 Admin Function Step Touchscreen operation Screen displays and notes The buttons in the Register user name screen have the  following functions. [Shift] button: Press to switch from lower case to upper case letters. [Clear] button: Deletes all characters in the User name field.
  • Page 110 3-4-6 Admin Function Step Touchscreen operation Screen displays and notes Again enter the PIN entered An Access level ● dialog box appears. in step 5 and press the [Enter] button. Access level dialog box An access level setting makes it possible to control access at ...
  • Page 111 3-4-6 Admin Function 2) Method for deleting users and changing registered information Step Touchscreen operation Screen displays and notes Press the [User The Admin screen ● replaces the user Management] button. management screen. Press the User field of the The selected user ●...
  • Page 112 3-4-6 Admin Function Step Touchscreen operation Screen displays and notes 4 -2 Press the [Change the The Select access ● level field appears. Access level] button to change the access level. Access level dialog Enter the user PIN you want to change and press the [Enter] Press the access level you ...
  • Page 113 3-4-6 Admin Function (2) User lockout User lockout when enabled, makes it possible to control user access at three different levels. 1) Method for setting user lockout Step Touchscreen operation Screen displays and notes The user that logs in must have [Administrator] privileges. Log in as a user as described ...
  • Page 114 3-4-6 Admin Function NOTE 1) Only a user logged in with [Administrator] privileges can enable the user lockout function. 2) Use of the user lockout function requires that users are registered. Register users as described in (1) "User Management." 3) When user lockout is enabled, a user must be logged in to use the touchscreen. 4) Once user lockout is enabled, only a user with an Administrator access level can call the Admin screen.
  • Page 115 3-4-6 Admin Function (3) Rotor management You can register the rotor and its serial number in the instrument to use it in setting operations and control total run time and number of runs. It is very important to manage the life of each rotor you use with the ultracentrifuge. The warranted life of the rotor is different depending on the type of rotor, the material the rotor is fabricated from and the policy of the manufacturer of the rotor.
  • Page 116 3-4-6 Admin Function 2) Automatic rotor deration You can register a rotor in the ultracentrifuge before use. After the rotor has been registered, you can utilize the rotor management feature of the ultracentrifuge each time a run is made using the rotor. When the registered rotor comes close to the end of its life, the ultracentrifuge indicates it by displaying a message on the screen.
  • Page 117 3-4-6 Admin Function 4) Registering a rotor A standard rotor can be registered when you use the rotor management feature of the ultracentrifuge. If the rotor has not been registered, the feature does not operate for the rotor. To register a rotor, use the procedure described below.
  • Page 118 3-4-6 Admin Function Step Touchscreen operation Screen displays and notes The Rotor Enter the serial number (S/N) ● Management screen and press the [Enter] button. appears. The added rotor and its serial number are displayed.  An "M" (for manual) appears at the right end of the field for ...
  • Page 119 3-4-6 Admin Function Step Touchscreen operation Screen displays and notes The Number of runs Press the [Edit Runs] button ● field appears. in the menu box. From the "Rotor log" The Rotor ● check the total number of management screen runs the rotor was used in the reappears and the other centrifuge, enter it and...
  • Page 120 3-4-6 Admin Function (3) Procedure for deleting a registered rotor Step Touchscreen operation Screen displays and notes The MENU box Press the Rotor management ● appears in the Rotor button, select the rotor to management screen. delete and press the [MENU] button.
  • Page 121 3-4-6 Admin Function (4) Run time indication Select either the elapsed time or remaining time as run time. Note that when the run time setting is set to [HOLD], elapsed time is automatically selected. 1) [Elapsed] button: Elapsed time is displayed in the centrifuge run time indication field in the Run screen and "Lapse"...
  • Page 122 3-4-6 Admin Function (6) Vacuum level You can select a vacuum level when acceleration starts after exceeding the vacuum waiting time at 4,000 rpm. Select Start high vacuum to prevent a temperature rise caused by wind. 1) [Medium] button: When the vacuum level in the rotor chamber reaches about 133 Pa, the vacuum waiting time ends and the rotor starts to accelerate to reach the set speed.

  • Page 123: Lan Communications

    3-4-6 Admin Function (8) LAN communications Connects and disconnects LAN communications. When the centrifuge is connected to a LAN, the "LogManager" software can be used for communications and storing operation results. To connect to a LAN, insert a LAN cable into the LAN port in the external connections on the right side of the instrument.

  • Page 124: Instrument Id, Service Contact Information

    3-4-7 Instrument ID, Service Contact Information 3-4-7 Instrument ID, Service Contact Information When multiple instruments are used, set separate IDs in each. Service contact information and other data can be stored as memos. Step Touchscreen operation Screen displays and notes Press the [ID/Contact] The instrument ID, ●...
  • Page 125 3-4-7 Instrument ID, Service Contact Information Step Touchscreen operation Screen displays and notes Press the MENU tab or the [X] button to return to the MENU Press the MENU tab, [X]  screen. button or the Run screen tab. Press the Run screen tab to display the Run screen. ...

  • Page 126: Happenings When Power Failure Occurs

    3-5 Happenings when Power Failure Occurs 3-5 Happenings when Power Failure Occurs DANGER: To avoid electrical shock hazards, follow below when servicing the centrifuge. 1) Be sure to turn off the POWER switch and turn off the distribution board of your centrifuge room if your centrifuge is equipped with a three wire power cord.
  • Page 127 3-5 Happenings when Power Failure Occurs (2) Turn off the POWER switch and power circuit breaker for the ultracentrifuge. (3) Remove the four M5 hexagon head bolts fixing the front cover from both sides and pull down the front cover by pulling its lower side forward. Then, remove the front cover. The upper side of the front cover is an insertion type.
  • Page 128 3-5 Happenings when Power Failure Occurs Handle Vacuum release screw Vacuum chamber Power breaker Door unlock wire Fig. 3-5-2. Ultracentrifuge with the front cover removed M5 hexagon head bolt for fixing the front cover (4 pcs.) Fig. 3-5-3. Installing the front cover (7) Turn on the power breaker.

  • Page 129: Maintenance

    4. Maintenance 4. Maintenance Be sure to read and keep in mind the following cautionary information before maintenance. DANGER: To avoid electrical shock hazards, follow below when servicing the centrifuge. 1) Be sure to turn off the POWER switch and turn off the distribution board of your centrifuge room if your centrifuge is equipped with a three wire power cord.

  • Page 130: Rotor Chamber

    4-1 Rotor Chamber 4-2 Drive Shaft (Crown) This centrifuge does not require complicated maintenance and inspection. For longer and safe use of this centrifuge without trouble, observe the following instructions. CAUTION: Using cleaning or sterilization method other than recommended in this instruction manual may cause corrosion or deterioration of this centrifuge.

  • Page 131: Cabinet

    4-3 Cabinet 4-4 Others 4-3 Cabinet Always keep the top deck and the cabinet of the centrifuge clean to prevent dust and other materials from falling into the rotor chamber. Wipe the top deck and the cabinet with a cloth or sponge dampened with a diluted solution of neutral detergent.

  • Page 132: Troubleshooting

    5. Troubleshooting 5. Troubleshooting Be sure to read and keep in mind the following cautionary information before troubleshooting. DANGER：To avoid electrical shock hazards, follow below when servicing the centrifuge. 1) Be sure to turn off the POWER switch and turn off the distribution board of your centrifuge room if your centrifuge is equipped with a three wire power cord.

  • Page 133: Alert Indicators

    5-1 Alert Indicators This ultracentrifuge incorporates a “self-diagnosis feature” that diagnoses the cause of any problem which may occur when you start the centrifuge or while in operation. 5-1 Alert Indicators If any trouble occurs, this machine gives a buzzer sound and displays an alert message in the FUNCTION area of the Run screen to warn of the trouble.
  • Page 134 5-1 Alert Indicators Alert Cause Action 1. Incorrect rotor is selected. • Select the correct rotor. SET ROTOR or 2. No rotor is installed. NO ROTOR • Install the rotor. 1. High room temperature • Lower the room temperature. ROOM TEMP 2.

  • Page 135: Diagnosed Problems-Requiring Maintenance

    5-2 Diagnosed Problems-Requiring Maintenance 5-3 User-Corrected Problem 5-2 Diagnosed Problems-Requiring Maintenance If any alert message between E11 and E86 appears, this ultracentrifuge requires maintenance by a service representative. When ordering a repair, inform us of the alert code you have received. NOTE When the "E13: Unexpected MPG pulse"...

  • Page 136: Installation

    6. Installation 6. Installation Installation or relocation of your centrifuge must be done by the authorized Thermo Fisher Scientific service representative. Contact your local dealer or a Thermo Fisher Scientific service representative. In order to use the ultracentrifuge fully and safely, follow the installation instructions given below. DANGER: To avoid electrical shock hazards, follow below when servicing the centrifuge.
  • Page 137 6. Installation (3) The ultracentrifuge requires a clearance of more than 20 cm at its rear and a floor space of at least 90 x 90 cm (See Fig.6-1). Ensure the surrounding of the instrument has a free circulation of air. Avoid installing the ultracentrifuge near any other heat-generating machine/equipment, which might reduce the ultracentrifuge cooling capacity.
  • Page 138 6. Installation 3. Fixing the safety cover The ultracentrifuge is shipped with the safety cover removed from its rear. When installing the ultracentrifuge, remove the packing and insert the lower part of the safety cover into the two hooks protruded from the frame and then secure it with the six M4 binding screws (See Fig.6-2). Safety cover (shipping status) Top deck Hook...
  • Page 139 6. Installation 5. Leveling (1) Turn the four leveling screws with a wrench to lift the caster about 10 to 20 mm off the floor as shown in Fig. 6-3. (2) Turn on the POWER switch and open the chamber door. Then turn off the switch again. If the power cord is not yet connected, remove the front cover and then open the door according to the instructions given in Section 3-5, "Happenings when power failure occurs."...

  • Page 140: Warranty For The Wx Plus Ultra Series Centrifuge

    7. Warranty for the WX Plus Ultra Series Centrifuge 7. Warranty for the Thermo Scientific Sorvall WX Plus Ultra Series Centrifuge THERMO FISHER SCIENTIFIC MAKES NO WARRANTY OF ANY KIND, EXPRESSED OR IMPLIED, INCLUDING THAT OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE EXCEPT AS STATED IN THIS WARRANTY POLICY STATEMENT.
  • Page 141 7. Warranty for the WX Plus Ultra Series Centrifuge THERMO FISHER SCIENTIFIC EXPRESSLY DISCLAIMS ANY LIABILITY TO ITS CUSTOMERS, DEALERS, AND REPRESENTATIVES, AND TO USERS OF ITS PRODUCTS, AND TO ANY OTHER PERSON OR PERSONS FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND AND FROM ANY CAUSE WHATSOEVER ARISING OUT OF OR IN ANY WAY CONNECTED WITH THE SALE, HANDLING, REPAIR, MAINTENANCE OR REPLACEMENT ARISING OUT OF OR IN ANY WAY CONNECTED WITH THE USE OF SAID PRODUCTS...

  • Page 142: Supply List

    8. Supply List 8. Supply List The items below are those supplied with the ultracentrifuge. Q'ty Item Name Part No. Item drawing Remarks Instruction manual Simulation CD ass’y “Compass” Software Supplied in 1-liter Vacuum pump oil NEO VAC 45128 Containers (MR100) M4 binding screw Hex.bar wrench Vacuum grease...
  • Page 143 Service Decontamination Policy If a centrifuge or rotor that has been used with radioactive or W A R N I N G pathogenic material requires servicing by Thermo personnel, either at the customer’s laboratory or at a Thermo facility, Because of the char- acteristics of the samples likely comply with the following procedure to ensure the safety of all to be processed, biolog- ical or...
  • Page 144 If the centrifuge to be serviced does not have a Decontamination Information Certificate attached and, in Thermo's opinion presents a potential radioactive or biological hazard, the Thermo representative will not service the equipment until proper decontamination and certification is complete. If Thermo receives a centrifuge at its Service facilities which, in its opinion, is a radioactive or biological hazard, the sender will be contacted for instructions as to disposition of the equipment.
  • Page 145 Thermo Electron LED GmbH Robert-Bosch-Straße 1 63505 Langenselbold Germany thermoscientific.com/centrifuge © 2014 Thermo Fisher Scientific Inc. All rights reserved. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.

Table of Contents